CBF beta-SMMHC, expressed in M4Eo AML, reduced CBF DNA-binding and inhibited the G1 to S cell cycle transition at the restriction point in myeloid and lymphoid cells

CBF beta-SMMHC is expressed from the inv(16) chromosome in M4Eo AML. Mice lacking CBF subunits or expressing the CBF beta-SMMHC or AML1-ETO oncoproteins failed to develop definitive hematopoiesis. To investigate these effects on hematopoiesis, we expressed CBF beta-SMMHC from the metallothionein promoter, in both 32D cl3 myeloid cells and Ba/F3 B-lymphoid cells. Addition of zinc increased CBF beta-SMMHC levels more than tenfold, with higher levels evident in Ba/F3 lines. Levels obtained in 32D cl3 cells were similar to those of endogenous CBF beta. Indirect immunofluorescence revealed zinc-inducible speckled, nuclear staining in Ba/F3 cells and diffuse nuclear staining in 32D cl3 cells. CBF beta-SMMHC reduced endogenous CBF DNA-binding fivefold in both cell types, increased cell generation time 1.9-fold, on average, in 32D cl3 cells and 1.5-fold in Ba/ F3 cells and decreased tritiated thymidine incorporation into DNA correspondingly. CBF beta-SMMHC increased the proportion of cells in G1 1.7-fold, on average, in 32D cl3 and Ba/F3 cells, and decreased the proportion of cells in S phase by a similar degree. CBF beta-SMMHC induced a marked increase in hypophosphorylated Rb, but did not alter IL-3 Receptor alpha or beta subunit levels. Neither apoptosis nor 32D differentiation was induced by zinc in IL-3 in these lines. Induction of CBF beta-SMMHC in 32D cl3 cells did not inhibit their differentiation to neutrophils or their expression of myeloperoxidase mRNA in G-CSF, and did not produce an eosinophilic phenotype. Additional, proliferative genetic changes in M4eo AMLs might potentiate inhibition of differentiation by CBF beta-SMMHC by allowing its increased expression.     

水布垭高砼面板堆石坝非线性和弹塑性分析

 主要介绍采用二维非线性和三维弹塑性有限元法对水布垭高砼面板堆石坝进行应力变形分析研究的成果。研究成果论证了设计方案的合理性和可行性。弹塑性模型由于能反映材料在高压应力条件下的剪缩特性，因而其成果更合理些。     

一种图文数据库系统的设计与实现

随着计算机技术的发展和水平的提高，图像，声音，图形等多媒体信息逐步应用于管理信息系统之中。文章中提出了图文数据库系统设计中存在的三个基本问题。讨论了介绍了图文数据库系统的设计方法和实现技术。     

炸药小药卷性能的初步研究

试验得出了几种炸药在不同药卷直径下的性能结果，并２锥形装药测定了炸药的熄爆直径。论文安泡乳化炸药的小药卷最适用于煤矿井下岩巷掘进的全断面爆破作业。     

基于Ansys的电化学加工辅助阴极设计

根据电化学加工极间间隙电势分布的拉普拉斯方程，提出了一种阴极设计算法－误差调整法。该算法的实现主要采用了Ansys有限元计算软件。计算结果表明：该法计算迭代次数较为适当，对于精度要求不高的电化学加工阴极设计，基于Ansys的辅助阴极设计有效可行。     

准连续Ti~(3+):Al_2O_3可调谐激光的研究

本文给出了Ti~(3+):Al_2O_3的准连续可调谐激光的实验结果。采用Nd:YAG内腔倍频532nm的准连续输出作为泵浦源,得到Ti~(3+):Al_2O_3的连续可调谐输出。波长覆盖范围为685nm到821nm。最大输出功率为293mW,斜率效率为20％,在805nm处的窄谱输出功率达240mW,总体转换效举为26％。     

通讯网的演进

在光纤高速传输技术、光交换与智能光网等与宽带接入、多媒体、3G等网络应用技术的推动下，建设基于IPv6协议的IP基础电信网成为可能。现在IP网正在朝着有序的、可管理的、有QoS保障的、可以支持各类业务的综合业务基础网演进。电信网也正在从网络接入层上、从长途网络层面上、从本地网络层面上、从固定网向含3G在内的移动网络等方面向支持IP技术及其综合业务的方面全面推进。     

三维结构可靠度对随机变量的敏感性研究

本文基于三维随机有限元提出了结构点可靠度、失效模式的可靠度及整个结构体系的可靠度分别对随机变量的＂分布参数＂和＂极限状态方程参数＂的敏感性计算方法，并以一典型重力坝为例进行了计算，得出了若干有益的结论。     

Fuzzy-Fusion Classifier for MMV Full-Polarization Radar Target Recognition Using One-Dimensional Scattering Centers

One-dimensional (1-D) scattering centers on a target can be used for radar target recognition purpose. In this paper, a new classifier based on fuzzy techniques is proposed, which is very much suitable for target recognition using 1-D scattering centers. Furthermore, information fusion techniques for full polarimetric receiver data are utilized at the decision level. The performance of the proposed fuzzy-fusion classifier is evaluated using a dataset of five aircraft models measured by millimeter-wave (MMW) full-polarization stepped-frequency radar in the compact range facility.     

烧结通用配料计算软件系统的开发

本文介绍了烧结通用配料计算软件系统的总体结构，软件的功能及其设计特点，该软件已在多个烧结经验中得到成功应用。