Oral administration of multispecies microbial supplements to sows influences the composition of gut microbiota and fecal organic acids in their post-weaned piglets

The timings of the administration of microbial supplements to control the populations of gut microbiota of piglets have been poorly understood. Here the effects of temporal administering multispecies microbial supplements to sows on the composition of gut microbiota and on the bacteria-mediated fecal metabolites in their offsprings were investigated. During gestation and lactation, pregnant sows were fed either a normal diet (group A) or a diet with multispecies supplements comprised of nine microbial species such as Lactobacillus delbrueckii subsp. bulgaricus, Bifidobacterium bifidum, Enterococcus faecium, Candida pintolopesii, and Aspergillus oryzae etc. (group B). All of the sows' piglets were temporarily fed with the same supplements around weaning in accordance with the guideline of the farm. This regimen was followed by a normal diet in both groups over one month thereafter. Under such conditions, the concentration of short-chain fatty acids (SCFAs) in fecal samples remarkably increased in group B compared to group A. When 16S rDNA sequences of the fecal bacteria were analyzed, the microbial structure of bacteria was different between both goups. Especially the Clostridium cluster IV and subcluster XIVa were particularly increased in group B, although the administered microbes were undetectable. Thus, temporal administration of multispecies-microbial supplements to pregnant sows changes the composition of SCFAs and gut microbiota in their offsprings.     

Biological treatment of wastewater discharged from biodiesel fuel production plant with alkali-catalyzed transesterification

The biological treatment of wastewater discharged from a biodiesel fuel (BDF) production plant conducting alkali catalysis transesterification was investigated. BDF wastewater has a high pH and high hexane-extracted oil and low nitrogen concentrations, and inhibits the growth of microorganisms. The biological treatment of BDF wastewater is difficult because the composition of such wastewater is not suitable for microbial growth. To apply the microbiological treatment of BDF wastewater using an oil degradable yeast, Rhodotorula mucilaginosa, the pH was adjusted to 6.8 and several nutrients such as a nitrogen source (ammonium sulfate, ammonium chloride or urea), yeast extract, KH2PO4 and MgSO4.7H2O were added to the wastewater. The optimal initial concentration of yeast extract was 1 g/l and the optimal C/N ratio was between 17 and 68 when using urea as a nitrogen source. A growth inhibitor was also present in the BDF wastewater, and this growth inhibitor could be detected by measuring the solid content in an aqueous phase after the hexane extraction of the wastewater. Microorganisms could not grow at solid contents higher than 2.14 g/l in the wastewater. To avoid the growth inhibition, the BDF wastewater was diluted with the same volume of water. Oil degradation in the diluted BDF wastewater was observed and the best result was obtained under the determined optimal conditions. This treatment system is simple because no controllers, except for a temperature, are necessary. These results suggest that the biological treatment system developed for BDF wastewater is useful for small-scale BDF production plants.     

Effect of oxidized and reduced forms of Escherichia coli DsbC on protein refolding

DsbC, which catalyzes disulfide isomerization, was overproduced in the periplasm of Escherichia coli and purified from the periplasmic fraction by osmotic shock and anion-exchange chromatography. The active site of the purified DsbC was found to be an oxidized form (ox-DsbC) which could be converted to the reduced form (red-DsbC) by the addition of dithiothreitol. The effect of ox- and red-DsbC on the refolding of chemically denatured and reduced proteins with different numbers of disulfide bonds and free cysteine-thiol groups was investigated. Ox-DsbC facilitated the refolding of proteins with multiple disulfide bonds in both oxidative and reductive environments, while red-DsbC facilitated refolding only in the former. On the other hand, only red-DsbC facilitated the refolding of proteins with multiple free cysteine-thiol groups but either form of DsbC did not facilitate the refolding of proteins with only one cysteine-thiol group. It is therefore important to choose the form which suits the properties of the protein. Holo-chaperonin from Thermus thermophilus and DsbC demonstrated a synergistic effect on protein refolding.     

Changes in the Composition of Triacylglycerols in the Fat Bodies of Bumblebee Males During Their Lifetime

The age-dependent changes in the composition of triacylglycerols (TAG) in the fat bodies of bumblebee males were studied using HPLC/MS. Two related species (Bombus terrestris and B. lucorum) were compared, with the age of the males being 0–30 days. The total amount of TAG in B. lucorum was about 2.7 times higher than that in B. terrestris for all of the ages studied. One to three-day-old males had the highest content of TAG in their fat bodies (1.6–2.3 mg/individual in B. terrestris and 3.8–4.2 mg/individual in B. lucorum). The analytical data show different patterns in both species. The qualitative composition of fatty acids in TAG was similar, but the mean relative abundance between B. terrestris and B. lucorum differed: 14:0, 7 and 14%; 16:0, 20 and 44%; 18:3, 62 and 23%; 18:1, 3 and 8%, respectively (the data is based on a GC/MS integration). A statistical evaluation of the dynamic changes in the TAG composition revealed that in B. terrestris different age classes were well separated according to their TAG composition while in B. lucorum the TAG did not change substantially during the male’s life. The TAG analyses provide more precise information on the differences between the classes studied than the FA composition alone.     

Effect of Curing Stress and Period on the Mechanical Properties of Cement-Mixed Sand

Cement mixing is one of the popular ground improvement technologies in geotechnical engineering practice. In order to effectively and confidently design cement-mixed soil structures for specific purposes, its stress-strain behavior needs to be well understood. Though there have been many studies on cement-mixed soils using different types of soils, their behaviors have not been generalized yet. As is the case with concrete materials, the hydration of cement in cement-mixed soil continues with time, thereby improving the strength and deformation characteristics of cement-mixed soil over time. In the field, the cementation bonds are formed under stress in case of in-situ soil. However, in the usual testing techniques, cementation bonds under stress has not been a point of consideration in most of the previous studies. This has led to an underestimation of the stress-strain behavior of cement-mixed soil. On the other hand, soils are subjected to confining stress during loading which has also some effect on the strength and deformation characteristics of soil which has not been considered yet in the case of cement-mixed sand. This study investigates the effect of curing stress and period on the strength and deformation characteristics of cement-mixed sand. The effect of confining stress in the triaxial test is also investigated in another series of specimens. A series of consolidated drained (CD) triaxial compression (TC) tests were done along with the small strain cyclic loading and bender element tests during monotonic loading to determine the small strain Young's modulus (E_v) and shear modulus (G_vh) respectively. The effect of the curing period is significant in the peak strength, stiffness, E_v, G_vh and also in the post peak regime. The curing stress also has a significant effect on the peak strength, E_v and G_vh. The confining stress has an effect on the peak strength, stiffness and in the post peak regime. However, the effect is small compared to clean sand.     

Reconstitution and function of Tetragenococcus halophila chaperonin 60 tetradecamer

Tetragenococcus halophila originally isolated from soy sauce is a halophilic lactic acid bacterium which can grow under 4 M sodium chloride. T. halophila chaperonin composed of a core moiety of chaperonin 60 (cpn60) and a lid moiety of chaperonin 10 (cpn10), is thought to contribute to host halotolerant capability. In this report, we reconstituted and characterized the core complex of T. halophila chaperonin by using a recombinant T. halophila cpn60 (Tcpn60) overexpressed in Escherichia coli. The reconstitution of Tcpn60 was performed in the presence of 10 mM MgCl2, 2 mM ATP and 0.8 M (NH4)2SO4 and the resultant oligomer was purified by gel filtration chromatography. Electron microscopy of the reconstituted Tcpn60 revealed a double toroidal tetradecameric structure that is characteristic of bacterial cpn60. The T. halophila tetradecamer cpn60 exhibited an ATPase activity and a refolding activity of both chemically and thermally denatured enolases under wide range of salt concentrations. Furthermore, we demonstrated that heterologous expression of Tcpn60 allowed the normal growth of host Escherichia coli cells under salt stress conditions and this effect was further enhanced by co-expression with Tcpn10. These results suggested that Tcpn60 contributes to the halotolerance property of T. halophila cell as a tetradecamer complex, probably associated with the Tcpn10 complex.     

THz Pulse Propagation on Microstrip Discontinuities

We investigated the propagation of THz pulses on such discontinuities as bends and branches in microstrip lines. Both the numerical and experimental results show that the transmission coefficient does not decrease severely up to 1 THz. As a result, the degradation in the pulse shape of one picosecond width is negligibly small. We also demonstrate a THz pulse splitter showing highly identical amplitude and phase spectra between the two arms.     

Variable bit-rate coding based on human visual system

One of the merits with the introduction of variable bit-rate coding to moving pictures is the constancy of picture quality. However, considering the fact that conventional SNR cannot reflect the result of subjective assessment sufficiently, other quality measures should be considered to design the variable bit-rate coders. In this paper, weighted SNR (WSNR), which is calculated by weighting coding errors with the spatial frequency response of human vision, is introduced as a quality measure in place of SNR, and the variable bit-rate coder based on this measure is considered. Then, subjective assessment is carried out and the effectiveness of our approach is confirmed.