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991.
Joachim Dirks 《Journal of the Society for Information Display》2001,9(3):233-235
Abstract— We will discuss the advantages and limitations of ac and dc lamp technology for microdisplay‐based projection systems. The performance of the lamp in terms of brilliance and thermal robustness together with the potential lamp lifetime for ac and dc lamps will be discussed. ac and dc lamp designs will be evaluated to judge their potential for future improvements in terms of usable projector lumens. 相似文献
992.
Opota DO Joachim G Kalantzis G Piccerelle P Reynier JP Joachim J 《Drug development and industrial pharmacy》1999,25(1):81-87
The development of a loading method of a water-soluble drug using aqueous binding solution to produce microgranules that were then coated with an aqueous ethylcellulose dispersion to sustain drug release is described. The results, in terms of drug used, showed that besides the fluidized bed parameters, the amount of drug dissolved in the binder solution plays an important role in obtaining a satisfying result during the spraying process. Thus, it seems necessary to determine the critical concentration above which the material started to adhere to the interior of the fluidization column, and the possibility of drug layering onto carrier material is aggravated. ANOVA of the time parameter for release of 63.2% of total drug (td) value showed significant influence of ethylcellulose (Aquacoat ECD-30) and dibutyl sebacate concentration on diphenhydramine hydrochloride (DPH) release. The dissolution rate decreased with an increase in polymer concentration. The diffusional exponent n of the Peppas equation indicated that the DPH release kinetic was non-Fickian but approached Fickian diffusion, particularly at higher coating levels. 相似文献
993.
The drawbacks of closed-loop current control for line- controlled converters are eliminated by generating close to optimal firing pulses in a feedforward channel. The steady-state and dynamic nonlinearities of the converter and the dc motor load are compensated. A standby closed-loopcurrent controller is activated only in the presence of disturbances. Experimental results show excellent dynamic performance at all operating points of a noncirculating current four quandrant drive. 相似文献
994.
Alexander T. Lill David X. Cao Max Schrock Joachim Vollbrecht Jianfei Huang Tung Nguyen-Dang Viktor V. Brus Brett Yurash Dirk Leifert Guillermo C. Bazan Thuc-Quyen Nguyen 《Advanced materials (Deerfield Beach, Fla.)》2020,32(33):1908120
PCPDTBT-SO3K (CPE-K), a conjugated polyelectrolyte, is presented as a mixed conductor material that can be used to fabricate high transconductance accumulation mode organic electrochemical transistors (OECTs). OECTs are utilized in a wide range of applications such as analyte detection, neural interfacing, impedance sensing, and neuromorphic computing. The use of interdigitated contacts to enable high transconductance in a relatively small device area in comparison to standard contacts is demonstrated. Such characteristics are highly desired in applications such as neural-activity sensing, where the device area must be minimized to reduce invasiveness. The physical and electrical properties of CPE-K are fully characterized to allow a direct comparison to other top performing OECT materials. CPE-K demonstrates an electrical performance that is among the best reported in the literature for OECT materials. In addition, CPE-K OECTs operate in the accumulation mode, which allows for much lower energy consumption in comparison to commonly used depletion mode devices. 相似文献
995.
996.
A general method is described that allows one to follow the surface display of recombinant proteins in Escherichia coli without having to use specific antibodies or enzymatic reactions. The method is based on cysteine-specific labeling through Michael addition to the double bond of maleimide and its derivatives, and takes advantage of the fact that naturally occurring surface proteins in E. coli contain no accessible cysteine residues. The method is easy to perform and could be simply applied to different analytic procedures including Western blot, spectral photometry, and flow cytometry. By using this new labeling method, single cells bearing a distinct protein at the surface could be selected by fluorescence-activated cell sorting. The data were obtained by using autodisplay, an efficient surface display system established for E. coli, but the method presented here represents rather a general solution for analyzing the surface display of recombinant proteins, independent of the cellular system used. 相似文献
997.
Enantiomer separation by countercurrent chromatography using cinchona alkaloid derivatives as chiral selectors 总被引:1,自引:0,他引:1
Franco P Blanc J Oberleitner WR Maier NM Lindner W Minguillón C 《Analytical chemistry》2002,74(16):4175-4183
Cinchona-derived anion-exchange-type chiral selectors have been adapted and employed in countercurrent chromatography (CCC) for the separation of enantiomers of N-derivatized amino acids and 2-aryloxypropionic acids. The accurate optimization of the enantioseparation in terms of solvent system composition, pH values, ionic strength, and CCC operating conditions was performed. A wide range of solvent mixtures was evaluated. Successful resolutions were achieved in systems such as ammonium acetate buffer/tert-amyl alcohol/methanol/heptane and especially ammonium acetate buffer/methyl isobutyl ketone or diisopropyl ether. Up to 300 mg (0.92 mmol) of N-(3,5-dinitrobenzoyl)-(+/-)-leucine was totally resolved in a single run using a 10 mM concentration of chiral selector in 122 mL of stationary phase. This amount could be increased up to 900 mg (2.77 mmol) when pH-zone-refining mode was applied. The results here presented account for the high potential of CCC as a preparative enantiomer separation technique. 相似文献
998.
The use of ring-disk ultramicroelectrodes as tips as a means of extending the methodology of the scanning electrochemical microscope is described. Electrodes consisting of one centered disk with six interconnected disks surrounding it served as the ring-disk electrodes. Basic experiments illustrate the behavior in the feedback mode and the generation collection mode. The dependence on the electrodes' size, both potentials, the tip-sample distance, and the local properties of the underying sample were studied. When approaching an electrode to the surface of a sample, steady-state collection efficiencies between zero and unity were elegantly altered by adjusting the distance between tip and sample. The shielding factor could be varied between 0.25 and 0.82 the same way. Concerning feedback methods, the results presented illustrate the new principle of applying a precisely located external stimulus as a separate electrochemical means of analyzing the sample's response. Obviously, this technique can be extended to irreversible redox mediators, which are not treated here explicitly, but give this concept even greater flexibility. The interpretation of such data can be deduced directly from this contribution. 相似文献
999.
Mechanistic and molecular investigations on stabilization of horseradish peroxidase C 总被引:2,自引:0,他引:2
Schmidt A Schumacher JT Reichelt J Hecht HJ Bilitewski U 《Analytical chemistry》2002,74(13):3037-3045
The enzyme horseradish peroxidase (HRP) shows a decreasing activity when the enzyme's substrate hydrogen peroxide is present with the degree of inactivation being dependent on the incubation time and the hydrogen peroxide concentration. Incubation times of some minutes do not inactivate the enzyme independent of the H2O2 concentration. After several hours, only 50% of the activity is found for a medium H2O2 excess, and a >100-fold excess of H2O2 completely inactivates the enzyme. Polymeric additives, in particular Gafquat, lead to higher residual activities, whereas stabilizers, such as aminopyrine, preserve the full activity. Circular dichroism (CD) measurements reveal that the enzyme structure remains more or less unchanged when hydrogen peroxide is added. Only when a 1000-fold excess of hydrogen peroxide is present are structural changes observed. UV spectra highlight that the heme group in the enzyme is affected by hydrogen peroxide in a first step. Without any prolonged incubation, a decrease of the Soret band to approximately 50% is found for low hydrogen peroxide concentrations (HRP/H2O2 from 1:1 to 1:100). Higher H2O2 concentrations lead to the formation of catalytically inactive HRP forms. Preincubation of Gafquat, which is a copolymer from vinylpyrrolidone and derivatized methyl methacrylate, with hydrogen peroxide shifts the influence of hydrogen peroxide to higher concentrations, the shift being dependent on the Gafquat concentration. This effect is not observed for other polymers, such as dextrans, but it is also found for the stabilizer aminopyrine. Extended incubation times (24 h) of HRP together with H2O2, however, lead to an at least partial recovery of the Soret band for lower H2O2 concentrations (H2O2/HRP from 1:1 to 1:100). When hydrogen peroxide is used in a >100 fold excess, the heme group is irreversibly destroyed, and even the characteristic band of cpd III is not found. Here, the presence of Gafquat only reduces the degree of destruction. Computer modeling of the interaction between the polymers and the enzyme shows no specific binding sites for the functional groups of the vinylpyrrolidone-methacrylate copolymer Gafquat or of DEAE-dextran on the enzyme, whereas for the only activating polymer, polyethylenimine clustering of binding sites is observed. 相似文献
1000.