全文获取类型
收费全文 | 339篇 |
免费 | 37篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 154篇 |
金属工艺 | 2篇 |
机械仪表 | 2篇 |
建筑科学 | 29篇 |
矿业工程 | 1篇 |
能源动力 | 7篇 |
轻工业 | 116篇 |
水利工程 | 2篇 |
石油天然气 | 3篇 |
无线电 | 3篇 |
一般工业技术 | 25篇 |
冶金工业 | 8篇 |
原子能技术 | 1篇 |
自动化技术 | 22篇 |
出版年
2024年 | 1篇 |
2023年 | 8篇 |
2022年 | 38篇 |
2021年 | 46篇 |
2020年 | 20篇 |
2019年 | 24篇 |
2018年 | 17篇 |
2017年 | 16篇 |
2016年 | 23篇 |
2015年 | 11篇 |
2014年 | 10篇 |
2013年 | 25篇 |
2012年 | 22篇 |
2011年 | 26篇 |
2010年 | 26篇 |
2009年 | 14篇 |
2008年 | 16篇 |
2007年 | 14篇 |
2006年 | 9篇 |
2005年 | 3篇 |
2004年 | 2篇 |
2003年 | 2篇 |
2002年 | 2篇 |
1997年 | 1篇 |
排序方式: 共有376条查询结果,搜索用时 0 毫秒
41.
Helena Albano Catarina Pinho Daniela Leite Joana Barbosa Joana Silva Luísa Carneiro Rui Magalhães Tim Hogg Paula Teixeira 《Food Control》2009,20(8):764-770
This study was conducted to evaluate the ability of Pediococcus acidilactici HA-6111-2, a PA-1 bacteriocin-producing lactic acid bacterium (LAB), isolated from “Alheira” to inhibit a cocktail of Listeria innocua strains during production and shelf-life of these products. The bacteriocinogenic culture reduced the Listeria population below the detection limit (1.5log CFU/g) and had no effect on the growth of the natural LAB flora or on the pH. Pathogenic organisms were not detected in any sample. The presence of some virulence factors and antibiotic resistances of the strain to be used as a bioprotective culture were investigated. P. acidilactici HA-6111-2 did not produce any of the biogenic amines tested; no formation of biofilms was observed; more l(+)lactic acid was produced than its isomer d(?); no gelatinase, DNase or lipase activity was recorded; no structural genes for the haemolysin, enterococcal surface protein, hydrolytic compounds, aggregation protein and cell-wall adhesins were detected, no significant antibiotic resistances were found. P. acidilactici HA-6111-2 appears to have potential as a bioprotective culture during “Alheira” fermentation. Moreover, a trained panel considered the protected product to be sensorially acceptable. 相似文献
42.
Juliana A. Barreto Peixoto Joana F. Silva M. Beatriz P. P. Oliveira Rita C. Alves 《Comprehensive Reviews in Food Science and Food Safety》2023,22(1):287-332
The coffee industry is one of the most important commercial value chains worldwide. Nonetheless, it is also associated to several social, economic, and environmental concerns that impair its sustainability. The present review is focused on these main sustainability concerns from the field to the coffee cup, as well as on the strategies that are being developed and/or implemented to attain sustainability and circular economy principles in the different chain segments. In this context, distinct approaches have been applied, such as sustainable certifications (e.g., voluntary sustainability standards), corporate sustainability initiatives, direct trade, relationship coffee concepts, geographical indication, legislations, waste management, and byproducts valorization, among others. These strategies are addressed and discussed throughout this review, as well as their recognized advantages and limitations. Overall, there is still a long way to go to attain the much-desired sustainability in the coffee chain, being essential to join the efforts of all actors and entities directly or indirectly involved, namely, producers, retailers, roasters, governments, educational institutions (such as universities and scientific research institutes), and organizations. 相似文献
43.
Joana Silva Celso Alves Alice Martins Patrícia Susano Marco Simes Miguel Guedes Stephanie Rehfeldt Susete Pinteus Helena Gaspar Amrico Rodrigues Mrcia Ines Goettert Amparo Alfonso Rui Pedrosa 《International journal of molecular sciences》2021,22(4)
Parkinsons Disease (PD) is the second most common neurodegenerative disease worldwide, and is characterized by a progressive degeneration of dopaminergic neurons. Without an effective treatment, it is crucial to find new therapeutic options to fight the neurodegenerative process, which may arise from marine resources. Accordingly, the goal of the present work was to evaluate the ability of the monoterpenoid lactone Loliolide, isolated from the green seaweed Codium tomentosum, to prevent neurological cell death mediated by the neurotoxin 6-hydroxydopamine (6-OHDA) on SH-SY5Y cells and their anti-inflammatory effects in RAW 264.7 macrophages. Loliolide was obtained from the diethyl ether extract, purified through column chromatography and identified by NMR spectroscopy. The neuroprotective effects were evaluated by the MTT method. Cells’ exposure to 6-OHDA in the presence of Loliolide led to an increase of cells’ viability in 40%, and this effect was mediated by mitochondrial protection, reduction of oxidative stress condition and apoptosis, and inhibition of the NF-kB pathway. Additionally, Loliolide also suppressed nitric oxide production and inhibited the production of TNF-α and IL-6 pro-inflammatory cytokines. The results suggest that Loliolide can inspire the development of new neuroprotective therapeutic agents and thus, more detailed studies should be considered to validate its pharmacological potential. 相似文献
44.
Melon seeds are an important source of bioactive compounds, which are considered to be health beneficial. Therefore, the objective of this work was to assess the impact of gaseous ozone treatments (30 and 60 min) on melon seeds paste (nonedible part) and compare with the effect on pulp (edible part). Ozone treatments were evaluated in terms of physicochemical (color, pH, and soluble solids content) and nutritional profiles (total phenolics, total carotenoids, and total antioxidant capacity) of processed material. Results indicate that ozone has a different impact on the two fruit matrices, being seeds less affected by this preservation treatment. 相似文献
45.
Ana S. Serras Srgio P. Cames Bernardo Antunes Vera M. Costa Flvio Dionísio Volkan Yazar Rui Vitorino Fernando Remio Matilde Castro Nuno G. Oliveira Joana P. Miranda 《International journal of molecular sciences》2021,22(23)
Doxorubicin (Dox) is one of the most widely used treatments for breast cancer, although limited by the well-documented cardiotoxicity and other off-target effects. Mesenchymal stem cell (MSC) secretome has shown immunomodulatory and regenerative properties, further potentiated under 3D conditions. This work aimed to uncover the effect of the MSC-derived secretome from 3D (CM3D) or 2D (CM2D) cultures, in human malignant breast cells (MDA-MB-231), non-tumor breast epithelial cells (MCF10A) and differentiated AC16 cardiomyocytes, co-treated with Dox. A comprehensive proteomic analysis of CM3D/CM2D was also performed to unravel the underlying mechanism. CM3D/CM2D co-incubation with Dox revealed no significant differences in MDA-MB-231 viability when compared to Dox alone, whereas MCF10A and AC16 viability was consistently improved in Dox+CM3D-treated cells. Moreover, neither CM2D nor CM3D affected Dox anti-migratory and anti-invasive effects in MDA-MB-231. Notably, Ge-LC-MS/MS proteomic analysis revealed that CM3D displayed protective features that might be linked to the regulation of cell proliferation (CAPN1, CST1, LAMC2, RANBP3), migration (CCN3, MMP8, PDCD5), invasion (TIMP1/2), oxidative stress (COX6B1, AIFM1, CD9, GSR) and inflammation (CCN3, ANXA5, CDH13, GDF15). Overall, CM3D decreased Dox-induced cytotoxicity in non-tumor cells, without compromising Dox chemotherapeutic profile in malignant cells, suggesting its potential use as a chemotherapy adjuvant to reduce off-target side effects. 相似文献
46.
47.
Miquel Antich-Rossell Maria Antnia Forteza-Genestra Marta Monjo Joana M. Ramis 《International journal of molecular sciences》2021,22(16)
Extracellular vesicles (EVs) present a great potential for the development of new treatments in the biomedical field. To be used as therapeutics, many different sources have been used for EVs obtention, while only a few studies have addressed the use of platelet-derived EVs (pEVs). In fact, pEVs have been shown to intervene in different healing responses, thus some studies have evaluated their regenerative capability in wound healing or hemorrhagic shock. Even more, pEVs have proven to induce cellular differentiation, enhancing musculoskeletal or neural regeneration. However, the obtention and characterization of pEVs is widely heterogeneous and differs from the recommendations of the International Society for Extracellular Vesicles. Therefore, in this review, we aim to present the main advances in the therapeutical use of pEVs in the regenerative medicine field while highlighting the isolation and characterization steps followed. The main goal of this review is to portray the studies performed in order to enhance the translation of the pEVs research into feasible therapeutical applications. 相似文献
48.
Ana Faria Diogo Pestana Joana Azevedo Fátima Martel Victor de Freitas Isabel Azevedo Nuno Mateus Conceição Calhau 《Molecular nutrition & food research》2009,53(11):1430-1437
Anthocyanins bioavailability is a major issue regarding their biological effects and remains unclear due to few data available on this matter. This work aimed to evaluate the absorption of anthocyanins at the intestine using Caco‐2 cells. Anthocyanin extract, rich in malvidin‐3‐glucoside, was obtained from red grape skins and tested on Caco‐2 cells. The absorption of anthocyanins, in absence or presence of 1% ethanol, was detected by HPLC/DAD/LC‐MS. Our results showed that this transport was significantly increased in the presence of ethanol especially after 60 min of incubation. In addition, cells that were pretreated for 96 h with anthocyanins (200 μg/mL) showed an increase of their own transport (about 50% increase). Expression of glucose transporters sodium‐dependent glucose transporter 1, facilitative glucose transporters 5, and facilitative glucose transporters 2 was assessed by RT‐PCR. It was found that facilitative glucose transporters 2 expression was increased (60%) in Caco‐2 cells pretreated with anthocyanins, by comparison with controls. When the effect of anthocyanin extract on 3H‐2‐deoxy‐D ‐glucose uptake was tested, an inhibitory effect was observed (about 60% decrease). However, the malvidin aglycone was tested and had no effect. In conclusion, anthocyanins could be absorbed through Caco‐2 cells, and can interfere with their own transport and also with glucose intestinal uptake. 相似文献
49.
António S. Barros Alexandra Nunes Joana Martins Ivonne Delgadillo 《Sensing and Instrumentation for Food Quality and Safety》2009,3(3):180-186
Fourier transform (FT) Near Infrared Spectroscopy (NIR) spectrometry in combination with partial least squares (PLS) regression
was used for direct, reagent-free determination fat and moisture content in milled olive and olive pomace. The two calibration
models obtained were built with samples from two years harvest (2006/2007) and have a good predictive power considering the
nature of the samples and are both being used in an industrial plant. 相似文献
50.
Alexandra Nunes Joana Martins António S. Barros Andrea C. Galvis-Sánchez Ivonne Delgadillo 《Sensing and Instrumentation for Food Quality and Safety》2009,3(3):187-191
Olive oil characteristics are directly related to olive quality. Information about olive quality is of paramount importance
to olive and olive oil producers, in order to establish its price. Real-time characterization of the olives avoids mixtures
of high quality with low quality fruits, and allows improvement of olive oil quality. This work describes an indirect determination
of olive acidity and that allows a rapid evaluation of olive oil quality. The applied method combines chemical analysis (30 min
Soxhlet olive pomace extraction) in tandem with a spectroscopic technique (FT-IR) and multivariate regression (PLS1). The
most suitable calibration model found used SNV pre-processing and was built with 4 Latent Variables giving a RMSECV of 8.7%
and a Q2 of 0.97. This accurate calibration model allows the estimation of olive acidity using a FT-IR spectrum of the corresponding
Soxhlet oil dry extract and therefore is a suitable method for indirect determination of FFA in olives. 相似文献