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Cover Feature: A Designed Enzyme Promotes Selective Post‐translational Acylation (ChemBioChem 15/2018) 下载免费PDF全文
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This Account describes a new paradigm for large-area nanoscale patterning that combines bottom-up and top-down approaches, merging chemistry with fabrication. This hybrid strategy uses simple nanofabrication techniques to control the alignment, size, shape, and periodicity of nanopatterns and chemical methods to control their materials properties and crystallinity. These tools are highly flexible and can create surface-patterned nanostructures with unusual properties and free-standing nanostructures that are multifunctional and monodisperse. The unprecedented scientific and technological opportunities enabled by nanoscale patterning over wafer-sized areas are discussed. 相似文献
74.
A Green Revolution for Africa is emerging after decades of neglect of Africa’s agricultural systems. To counter these years of neglect, the then United Nations Secretary-General Kofi Annan called for “a uniquely African Green Revolution”. Since then, a number of initiatives have emerged or are emerging to realize this important vision. As more money and attention galvanizes much-needed action on the African Green Revolution, a vigorous debate is required to ensure that the mission of improving food security on the world’s poorest continent is achieved in the most effective, comprehensive and inclusive manner possible. The African Green Revolution cannot be limited to increasing yields of staple crops but must be designed as a driver of sustainable development, which includes gender empowerment and nutrition elements. This paper first reviews the Asian Green Revolution’s successes and shortcomings from a nutrition and gender perspective and then outlines what the global community can do to ensure that some of the limitations of the Asian Green Revolution, specifically with regard to nutrition and gender, are not repeated. 相似文献
75.
Heather A. Doty Megan R. Leedy Harry S. Courtney Warren O. Haggard Joel D. Bumgardner 《Journal of materials science. Materials in medicine》2014,25(6):1449-1459
A biodegradable, composite bone graft, composed of chitosan microspheres embedded in calcium sulfate, was evaluated in vitro for point-of-care loading and delivery of antibiotics and growth factors to prevent infection and stimulate healing in large bone injuries. Microspheres were loaded with rhBMP-2 or vancomycin prior to mixing into calcium sulfate loaded with vancomycin. Composites were evaluated for set time, drug release kinetics, and bacteriostatic/bactericidal activity of released vancomycin, induction of ALP expression by released rhBMP-2, and interaction of drugs on cells. Results showed the composite set in under 36 min and released vancomycin levels that were bactericidal to S. aureus (>MIC 8–16 μg/mL) for 18 days. Composites exhibited a 1 day-delayed release, followed by a continuous release of rhBMP-2 over 6 weeks; ranging from 0.06 to 1.49 ng/mL, and showed a dose dependent release based on initial loading. Released rhBMP-2 levels were, however, too low to induce detectable levels of ALP in W20-17 cells, due to the affinity of rhBMP-2 for calcium-based materials. With stimulating amounts of rhBMP-2 (>50 ng/mL), the ALP response from W-20-17 cells was inhibited when exposed to high vancomycin levels (1,800–3,600 μg/mL). This dual-delivery system is an attractive alternative to single delivery or preloaded systems for bone regeneration since it can simultaneously fight infection and deliver a potent growth factor. Additionally, this composite can accommodate a wide range of therapeutics and thus be customizable for specific patient needs, however, the potential interactive effects of multiple agents must be investigated to ensure that functional activity is not altered. 相似文献
76.
Schwartz Sonia; Hoyte Joel; James Thea; Conoscenti Lauren; Johnson Renee; Liebschutz Jane 《Canadian Metallurgical Quarterly》2010,2(1):54
A dearth of literature exists on barriers to conducting research with Black male victims of community violence, despite the need for evidence-based postinjury interventions. This study used qualitative data from a cross-sectional interview study (n = 16) and a pilot intervention study (n = 11) conducted in Boston, MA to identify challenges and facilitators to conducting research with Black male victims of community violence, particularly with regard to recruitment and maintenance of a study sample. Qualitative methods, including Grounded Theory and ethnography, were used to analyze the data. Challenges included a fear of police involvement, an impression of “snitching” when disclosing personal information, mistrust of research motives, suspicion of the informed consent process, the emotional impact of the trauma itself, and logistical issues. Facilitators to research included monetary incentives and motivation to help oneself and others. Participant recommendations on recruitment methods relating to approach and timing are provided. Findings from this study may assist in the planning of research studies for Black male victims of community violence. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
77.
This study examines the impacts of energy price changes resulting from different carbon-pricing policies on the competitiveness of selected US energy-intensive industries. It further examines possible industry responses, and identifies and provides a preliminary evaluation of potential opportunities to mitigate these impacts. The industry sectors investigated – steel, aluminum, chemicals and paper – are among the largest industrial users of fossil fuels in the US economy. The results of this examination show that climate policies that put a price on carbon could have substantial impacts on the competitiveness of US energy-intensive manufacturing sectors over the next two decades, if climate regulations are applied only in the United States, and no action is taken to invest in advanced low- and no-carbon technologies. The extent of these impacts will vary across industries, depending on their energy intensities, the mix of energy sources they rely on and how energy is used in production activities (heat and power, feedstock). Of relevance is also the speed and rigor with which industries adopt new technologies and retire (or replace) old ones. Other factors affecting these impacts include an industry's vulnerability to foreign imports and its ability to pass through cost increases to its customers in the face of international market competition. 相似文献
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Electronic excitation of a probe chromophore can lead to a change in dipole moment that influences its activity or solubility in solution and changes its relative affinity for partitioning between two phases. Photoexcitation of a probe molecule can, therefore, perturb a sorption equilibrium, and the relaxation kinetics of the probe to the new equilibrium conditions can be monitored in a time-resolved luminescence experiment. The adsorption/desorption kinetics of rose bengal, distributed between a C-18 derivatized porous-silica surface and a liquid mobile-phase solution, were investigated. These kinetics were determined by observing their effect on the phosphorescence decay of the triplet state of rose bengal and its quenching by ferricyanide. The methanol/water solvent compositions were varied to alter the fraction of adsorbed rose bengal. The adsorption rate constant for the triplet state was determined from the dependence of the phosphorescence relaxation rate on dye concentration in solution. The results indicate that the adsorption kinetics are diffusion controlled and that the relaxation is influenced by efficient triplet-energy transfer between excited- and ground-state rose bengal at the C-18 silica/solution interface. 相似文献
80.
In this paper, the optimal filtering problem for polynomial system states with polynomial multiplicative noise over linear observations is treated proceeding from the general expression for the stochastic Ito differential of the optimal estimate and the error variance. As a result, the Ito differentials for the optimal estimate and error variance corresponding to the stated filtering problem are first derived. The procedure for obtaining a closed system of the filtering equations for any polynomial state with polynomial multiplicative noise over linear observations is then established, which yields the explicit closed form of the filtering equations in the particular cases of a linear state equation with linear multiplicative noise and a bilinear state equation with bilinear multiplicative noise. In the example, performance of the designed optimal filter is verified for a quadratic state with a quadratic multiplicative noise over linear observations against the optimal filter for a quadratic state with a state‐independent noise and a conventional extended Kalman–Bucy filter. Copyright © 2006 John Wiley & Sons, Ltd. 相似文献