Influence of type of food on the kinetics and overall production of Bacillus cereus emetic toxin

Potato puree and penne pasta were inoculated with cereulide producing B. cereus 5964a and B. cereus NS117. Static incubation at 28 degrees C proved these two foods to be a better substrate for higher cereulide production (4,080 ng/g in puree and 3,200 ng/g in penne were produced by B. cereus 5964a during 48 h of incubation) compared with boiled rice (2,000 ng/g). This difference occurred despite B. cereus counts of more than 10(8) CFU/g in all three products. Aeration of cultures had a negative effect on cereulide production, causing concentrations more than 10-fold lower than in some statically incubated samples. Cereulide production remained undetectable in shaken milk, whereas it reached 1,140 ng/ml in statically incubated milk. At 12 and 22 degrees C, presence of background flora was also a determinative factor. A total B. cereus count of more than 106 CFU/ml did not necessarily lead to uniform cereulide production and was also dependent on the B. cereus strain involved. In this study, we confirm that a number of factors play a crucial role in the determination of the extent to which, if at all, cereulide will be produced. Among those, type of the food, temperature, pH, and whether additional aeration (via incubation on an orbital shaker) is induced had an important role. An important effect was also induced by the cereulide-producing strain involved.     

Enantioselective formation of methyl sulfone metabolites of 2,2',3,3',4,6'-hexachlorobiphenyl in rat

Several nonsymmetric polychlorinated biphenyl (PCB) congeners form atropisomers due to steric hindrance of free rotation around the phenyl-phenyl bond. It is evident from the literature that both chiral PCB congeners and their atropisomeric methylsulfonyl-PCB metabolites, formed in higher animals and in humans, are present in biota as nonracemic mixtures. Chiral methylsulfonyl-PCBs are strongly dominated by one of the atropisomers in mammalian tissues. The aim of the present study is to examine enantioselective metabolism, retention, and excretion of 2,2',3,3',4,6'-hexachlorobiphenyl (CB-132) in rat by administration of a CB-132 racemate and pure atropisomers. Chemical analysis of liver, lung, and adipose tissue from the rats showed a strong retention of one of the CB-132 atropisomers and a similar, but even more pronounced, accumulation of one of the atropisomers of the meta- and para-methylsulfonyl-substituted CB-132 metabolites in these tissues. Metabolites with R structures were predominately formed from one of the atropisomers of CB-132. The slower metabolism of the other atropisomer of CB-132 and its pronounced excretion in feces suggest an enantioselective metabolism. The results indicate enantio-selective formation of the methylsulfonyl-CB132 metabolites and confirm the critical role of stereochemistry of chemicals for their metabolism.     

Potential gain in photocurrent generation for Cu(In,Ga)Se2 solar cells by using In2O3 as a transparent conductive oxide layer

This study highlights the potential of atomic layer deposited In₂O₃ as a highly transparent and conductive oxide (TCO) layer in Cu(In,Ga)Se₂ (CIGSe) solar cells. It is shown that the efficiency of solar cells which use Zn‐Sn‐O (ZTO) as an alternative buffer layer can be increased by employing In₂O₃ as a TCO because of a reduction of the parasitic absorption in the window layer structure, resulting in 1.7 mA/cm² gain in short circuit current density (J_sc). In contrast, a degradation of device properties is observed if the In₂O₃ TCO is combined with the conventional CdS buffer layer. The estimated improvement for large‐scale modules is discussed. Copyright © 2015 John Wiley & Sons, Ltd.     

Partially hydrolyzed whey proteins prevent clinical symptoms in a cow's milk allergy mouse model and enhance regulatory T and B cell frequencies

1 Scope

Partially hydrolyzed cow's milk proteins are used to prevent cow's milk allergy in children. Here we studied the immunomodulatory mechanisms of partial cow's milk hydrolysates in vivo.

2 Methods and results

Mice were sensitized with whey or partially hydrolyzed whey using cholera toxin. Whey‐specific IgE levels were measured to determine sensitization and immune cell populations from spleen, mesenteric lymph nodes and Peyer's patches after oral whey administration were measured by flowcytometry. Whey‐specific IgE and IgG1 levels in partial whey hydrolysate sensitized animals were enhanced, but challenge did not induce clinical symptoms. This immunomodulatory effect of partial whey hydrolysate was associated with increased regulatory B and T cells in the spleen, together with a prevention of IgM‐IgA class switching in the mesenteric lymph nodes and an increased Th1 and activated Th17 in the Peyer's patches.

3 Conclusion

Partial hydrolysate sensitization did not induce whey‐induced clinical symptoms, even though sensitization was established. Increased regulatory cell populations in the systemic immune system and a prevention of increased total Th1 and activated Th17 in the intestinal immune organs could contribute to the suppression of allergic symptoms. This knowledge is important for a better understanding of the beneficial effects of hydrolysates.     

Effect of water activity and temperature on growth and the relationship between fumonisin production and the radial growth of Fusarium verticillioides and Fusarium proliferatum on corn

The two major fumonisin-producing Fusarium species are Fusarium verticillioides and Fusarium proliferatum. The growth and fumonisin production of these two isolates on corn was studied at water activities (a(w)) between 0.860 and 0.975 and at temperatures between 15 and 30 degrees C. Growth rates (g, mm/day) were obtained by linear regression during the linear phase of growth. In general, growth rates for both isolates increased significantly (P < 0.05) with increases in a(w) and temperature. Both fumonisin production and radial growth (mycelial development) for both isolates increased with a(w) at all temperatures investigated, but the effect of temperature on this relationship was not obvious. The effect of temperature on fumonisin production at high a(w) values optimal for growth was only marginal, whereas at lower a(w) values the effect of temperature was more pronounced, with more fumonisin production occurring at temperatures not optimal for growth. The optimum temperature for fumonisin production was between 15 and 25 degrees C. For F. proliferatum, the optimum temperature for growth at all a(w) values, 30 degrees C, resulted in the poorest fumonisin production. For both isolates, the slowest initial rate of fumonisin production was at 15 degrees C, the temperature at which the slowest growth rates were obtained.     

Influence of storage conditions of apples on growth and patulin production by Penicillium expansum

Penicillium expansum causes blue mould rot, a serious post-harvest disease of apples, and is the main producer of the mycotoxin patulin. The present study aimed to determine the influence of storage conditions (i.e. temperature and O(2) level) on growth and patulin production by different P. expansum strains on a simulation medium and on apples. Growth was strongly influenced by the temperature, while the used atmosphere (20, 3, and 1% O(2); <1% CO(2)) had no effect. Optimal growth was observed at 25 degrees C for every strain tested. Patulin production was stimulated when the temperature decreased (from 20 to 10 or 4 degrees C), while a further decrease of the temperature to 1 degrees C caused a reduction in patulin production. The temperature at which the stimulation was changed into suppression was strain dependent. Similar results were observed for the O(2) level. A reduction of the O(2) level from 20 to 3% O(2) could stimulate or suppress patulin production depending on the strain, while a clear decrease of the patulin production was observed when the O(2) level was reduced from 3 to 1%. These results show that the induction of limited stress to the fungus, such as lowering the temperature or lowering the O(2) levels stimulates patulin production. However, the combination of different stress conditions (e.g. low temperature and low O(2)) will result in a reduced formation of the toxin. The combination of stress conditions, at which the transition from stimulation to suppression is observed, is strain dependent. Moreover, patulin production is characterized by a high natural variability. The presented results show that the temperature and O(2) level has to be as low as possible during the storage of apples in order to suppress patulin production and to guarantee food safety.     

Dynamic Modeling of a Parabolic Trough Solar Thermal Power Plant with Thermal Storage Using Modelica

Concentrating solar power (CSP) technology with thermal energy storage is a renewable and emerging technology. In this work, dynamic models for analyzing and evaluating energy storage concepts and its interaction with the solar field and the power block have been developed. A physical model of a 50 MW CSP plant has been implemented in the modeling language Modelica. The models are developed in a modular, flexible structure with a well-defined interface to easily replace and test modules of various detail and complexity. Models include turbine island, steam generator, solar field, and thermal energy storage system. In addition, a decentralized control configuration has been developed. Results have been successfully validated against the reference plant key steady-state data. Dynamic response of the power block has shown expected behavior, and transient durations were comparable with settling times predicted in literature. Furthermore, the performance of the plant has been evaluated during a typical summer day including effects such as variation of solar irradiance, charging and discharging the heat storage system, and dumping excess heat in the solar field. The summer day scenario results agreed with published performance of the plant.     

Review on fermentative biohydrogen production from water hyacinth,wheat straw and rice straw with focus on recent perspectives

Hydrogen (H₂) is often considered as the best option to store energy coming from renewable sources. Hydrogen production from lignocellulosic biomass via fermentation offers low cost and environmental friendly method in terms of energy balance and provides a sustainable pathway for utilization of huge amount of unused biomass. In this regard, special attention on potential of different lignocellulosic biomass is required. In this paper, the fermentative hydrogen production from three carbohydrates-rich biomass: water hyacinth, wheat straw and rice straw is comprehensively reviewed. In other point of view, usage of H₂ has a 10% growth annually that will reach to 8–10% of total energy in 2025. Furthermore, research on recent trends of fermentative hydrogen production is crucial and vital. However, the majority of the published researches in the last decade confirmed that some challenges exists which are the process optimization, effecting parameters and commercialization aspects.