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Dumitrita Iftime Martin Jasyk Andreas Kulik Prof. Dr. Johannes F. Imhoff Dr. Evi Stegmann Prof. Dr. Wolfgang Wohlleben Prof. Dr. Roderich D. Süssmuth Dr. Tilmann Weber 《Chembiochem : a European journal of chemical biology》2015,16(18):2615-2623
Lanthipeptides are ribosomally synthesized and post‐translationally modified microbial secondary metabolites. Here, we report the identification and isolation of streptocollin from Streptomyces collinus Tü 365, a new member of class IV lanthipeptides. Insertion of the constitutive ermE* promoter upstream of the lanthipeptide synthetase gene stcL resulted in peptide production. The streptocollin gene cluster was heterologously expressed in S. coelicolor M1146 and M1152 with 3.5‐ and 5.5‐fold increased yields, respectively. The structure and ring topology of streptocollin were determined by high resolution MS/MS analysis. Streptocollin contains four macrocyclic rings, with one lanthionine and three methyllanthionine residues. To the best of our knowledge, this is the first report on the isolation of a class IV lanthipeptide in preparative amounts, and on the successful heterologous expression of a class IV lanthipeptide gene cluster. 相似文献
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Recent literature suggests that multinational companies pursue regional rather than global strategies. Therefore, this study investigates regional management structures, using French multinational companies (MNCs) in the Asia–Pacific region as an empirical context, to address two research questions: first, do MNCs split Asia into subregions and, if so, what are the resulting clusters of countries and clustering criteria? Second, what kind of regional management structures do MNCs establish in Asia, and what are their roles and functions? Factors, such as MNC size, the size of host markets, or the nature of their activities, might explain some differences. The authors conducted 77 face-to-face interviews with expatriated managers in charge of the subsidiaries or regional management structures of 47 French MNCs located in 11 countries in Asia, then crossed these data with secondary sources of information. Nearly half the MNCs subdivide the Asia–Pacific region into clusters of countries, where they locate regional management centres (regional headquarters, regional offices, distribution centres, local offices) with substantial functions and roles. The main drivers of a regional Asian strategy and organisation are the overall size of the MNC and its sales in Asia; the presence of manufacturing activities does not exert any influence. This research identifies ten clusters of countries in Asia, determined by the French MNCs in our sample, on the basis of four main criteria: market orientation/economic logic, geographical and institutional proximity, cultural differences, and the MNC’s own characteristics. Smaller MNCs do not slice Asia into clusters but rather centralise regional decisions and control procedures, implementing few regional management centres in Asia and giving them limited roles and functions. 相似文献
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Native arteriovenous fistula placement in three patients after implantation of a left ventricular assist device with non‐pulsatile blood flow
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Johannes F. Schaefers Christian Ertmer 《Hemodialysis international. International Symposium on Home Hemodialysis》2017,21(3):E54-E57
Patients with left ventricular assist device have an increased risk for postoperative acute kidney injury and long‐term requirement of dialysis. As a chronic access for dialysis, native arteriovenous fistulae are uncommon options due to suspected maturation failure. We present three patients who received successful arteriovenous fistulae placement after left ventricular assist device implantation with assisted long term patency. 相似文献
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An evaluation approach for correspondence-driven domains is suggested and implemented. Touch screen and trackball controls were evaluated as interaction devices for large-area displays in the cockpits of highly agile jet aircraft. To account for the context conditions of selected use cases, informatory quality and the difficulty of situational demands were analysed and manipulated experimentally in dual-task scenarios, which were completed by experienced pilots. Results indicate a clear performance advantage of touch compared to trackball interaction, accompanied by less workload. Informatory dimensions induce different performance and workload ratings. Cognitive demands interfere the least with aiming performance, followed by visual and motor. Task and device influences are interdependent. Motor components of an additional task interfere especially with trackball control actions. Workload operates as a buffer. When difficulty increases, performance decrements are lower than workload increments. It is argued that this cognitive manipulation of informatory context is advisable for correspondence-driven domains, where context is expected to influence human–machine interaction. Transfer to automotive display evaluation appears to be straightforward. 相似文献
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Johannes Kahl Farnaz Alborzi Alexander Beck Susanne Bügel Nicolaas Busscher Uwe Geier Darja Matt Tabea Meischner Flavio Paoletti Sirli Pehme Angelika Ploeger Ewa Rembiałkowska Otto Schmid Carola Strassner Bruno Taupier‐Letage Aneta Załęcka 《Journal of the science of food and agriculture》2014,94(13):2582-2594
In 2007 EU Regulation (EC) 834/2007 introduced principles and criteria for organic food processing. These regulations have been analysed and discussed in several scientific publications and research project reports. Recently, organic food quality was described by principles, aspects and criteria. These principles from organic agriculture were verified and adapted for organic food processing. Different levels for evaluation were suggested. In another document, underlying paradigms and consumer perception of organic food were reviewed against functional food, resulting in identifying integral product identity as the underlying paradigm and a holistic quality view connected to naturalness as consumers' perception of organic food quality. In a European study, the quality concept was applied to the organic food chain, resulting in a problem, namely that clear principles and related criteria were missing to evaluate processing methods. Therefore the goal of this paper is to describe and discuss the topic of organic food processing to make it operational. A conceptual background for organic food processing is given by verifying the underlying paradigms and principles of organic farming and organic food as well as on organic processing. The proposed definition connects organic processing to related systems such as minimal, sustainable and careful, gentle processing, and describes clear principles and related criteria. Based on food examples, such as milk with different heat treatments, the concept and definitions were verified. Organic processing can be defined by clear paradigms and principles and evaluated according criteria from a multidimensional approach. Further work has to be done on developing indicators and parameters for assessment of organic food quality. © 2013 Society of Chemical Industry 相似文献