Yield and transient effects during the plastic deformation of solid polymers

Tensile tests were performed on seven commercial polymers at 22° C and at constant true strain rates of 10^–4 to 10^–1 sec^–1. The constant strain rates were imposed on the minimum section of each sample with the aid of a diametral transducer, an exponential function generator and a closed-loop hydraulic testing machine. The polymers investigated were: high and low density polyethylene, polytetrafluoroethylene, polypropylene. polyvinylchloride and polyamide 6 and 66. True yield drops were observed in the rigid glassy polymers, whereas yielding was more gradual in the semi-crystalline or plasticized polymers. Strain rate change tests were also performed, during which one order of magnitude increases and decreases were imposed on the specimens. Normal transients were observed at small strains in the samples containing a rubbery phase, while the transients were of an inverse nature in the samples containing a glassy phase. With an increase in the strain at which the change was initiated, the normal transients changed in character to inverse. Transient tests were also performed in which straining was interrupted to permit a period of stress relaxation or of holding in the unloaded condition prior to the resumption of straining. A quantitative model is proposed, based on the dynamics of plastic waves which accounts for the transition from normal to inverse transient behaviour with increasing strain, and also explains the opposite effects of stress relaxation and of specimen unloading on the restraining transients.     

Navigation Aided Image Processing in UAV Surveillance: Preliminary Results and Design of an Airborne Experimental System

This paper describes an airborne reconfigurable measurement system being developed at Swedish Defence Research Agency (FOI), Sensor Technology, Sweden. An image processing oriented sensor management architecture for UAV (unmanned aerial vehicles) IR/EO-surveillance is presented. Some preliminary results of navigation aided image processing in UAV applications are demonstrated, such as SLAM (simultaneous localization and mapping), structure from motion and geolocation, target tracking, and detection of moving objects. The design goal of the measurement system is to emulate a UAV-mounted sensor gimbal using a stand-alone system. The minimal configuration of the system consists of a gyro-stabilized gimbal with IR and CCD sensors and an integrated high-performance navigation system. The navigation system combines dGPS real-time kinematics (RTK) data with data from an inertial measurement unit (IMU) mounted with reference to the optical sensors. The gimbal is to be used as an experimental georeferenced sensor platform, using a choice of carriers, to produce military relevant image sequences for studies of image processing and sensor control on moving surveillance and reconnaissance platforms. Furthermore, a high resolution synthetic environment, developed for sensor simulations in the visual and infrared wavelengths, is presented. © 2004 Wiley Periodicals, Inc.     

Towards 3D Bioprinted Spinal Cord Organoids

Three-dimensional (3D) cultures, so-called organoids, have emerged as an attractive tool for disease modeling and therapeutic innovations. Here, we aim to determine if boundary cap neural crest stem cells (BC) can survive and differentiate in gelatin-based 3D bioprinted bioink scaffolds in order to establish an enabling technology for the fabrication of spinal cord organoids on a chip. BC previously demonstrated the ability to support survival and differentiation of co-implanted or co-cultured cells and supported motor neuron survival in excitotoxically challenged spinal cord slice cultures. We tested different combinations of bioink and cross-linked material, analyzed the survival of BC on the surface and inside the scaffolds, and then tested if human iPSC-derived neural cells (motor neuron precursors and astrocytes) can be printed with the same protocol, which was developed for BC. We showed that this protocol is applicable for human cells. Neural differentiation was more prominent in the peripheral compared to central parts of the printed construct, presumably because of easier access to differentiation-promoting factors in the medium. These findings show that the gelatin-based and enzymatically cross-linked hydrogel is a suitable bioink for building a multicellular, bioprinted spinal cord organoid, but that further measures are still required to achieve uniform neural differentiation.     

Alpha-Synuclein Autoimmune Decline in Prodromal Multiple System Atrophy and Parkinson’s Disease

Multiple-system trophy (MSA) and Parkinson’s Disease (PD) are both progressive, neurodegenerative diseases characterized by neuropathological deposition of aggregated alpha-synuclein (αSyn). The causes behind this aggregation are still unknown. We have reported aberrancies in MSA and PD patients in naturally occurring autoantibodies (nAbs) against αSyn (anti-αSyn-nAbs), which are important partakers in anti-aggregatory processes, immune-mediated clearance, and anti-inflammatory functions. To elaborate further on the timeline of autoimmune aberrancies towards αSyn, we investigated here the Immunoglobulin (Ig) affinity profile and subclass composition (IgG-total, IgG1-4 and IgM) of anti-αSyn-nAbs in serum samples from prodromal (p) phases of MSA and PD. Using an electrochemiluminescence competition immunoassay, we confirmed that the repertoire of high-affinity anti-αSyn-nAbs is significantly reduced in pMSA and pPD. Further, we demonstrated that pPD had increased anti-αSyn IgG-total levels compared to pMSA and controls, concordant with increased anti-αSyn IgG1 levels in pPD. Anti-αSyn IgG2 and IgG4 levels were reduced in pMSA and pPD compared with controls, whereas anti-αSyn IgG3 levels were reduced in pMSA compared to pPD and controls. The results indicate that the impaired reactivity towards αSyn occurs prior to disease onset. The apparent lack of high-affinity anti-αSyn nAbs may result in reduced clearance of αSyn, leading to aggregation of the protein. Thus, this study provides novel insights into possible causes behind the pathogenesis in synucleinopathies such as MSA and PD.     

Effect of crude oil ageing on low salinity and low salinity surfactant flooding

Injection of brine with lower salinity than the connate brine has proven to give a moderate increase in oil recovery in sandstones. Recent research has shown that this process will significantly benefit from introducing surfactant optimised for low salinity environment.The mechanisms underlying increased recovery by low salinity brine injection are not yet fully understood. However, research to date suggests that they are related to complex crude oil/brine/rock interactions. With this in mind, the present paper investigates primarily how the extent of oil recovery from Berea sandstones subjected to long term exposure of crude oil is influenced by (1) low salinity water injection and (2) combined process low salinity water injection with surfactant flooding.Core displacement tests were conducted on four Berea cores (30 cm), two in a natural state and two that had been subject to extensive crude oil ageing at high temperature. Results obtained from different flooding steps are discussed in terms of oil recovery and effluent properties including turbidity, pH- and ion analysis (Na⁺, Mg²⁺, Ca²⁺). The results effectively illustrate that oil recoveries from the aged cores are higher during both low salinity water injection and low salinity water injection combined with surfactant flooding. An assessment of how tertiary oil recovery in aged and unaged cores varies with surfactant concentration is also presented.Effluent ion analysis from low salinity water floods showed that Mg²⁺ ions were strongly retained in the aged core while Ca²⁺ ions were being produced from both aged and unaged cores. The latter was attributed mainly to calcite dissolution. Results obtained from pressure profiles, effluent ion analysis and turbidity tests suggest higher production and elution of fine particles from the unaged core.     

Enabling Coarse X-ray Fluorescence Imaging Scans with Enlarged Synchrotron Beam by Means of Mosaic Crystal Defocusing Optics

Trace elements, functionalized nanoparticles and labeled entities can be localized with sub-mm spatial resolution by X-ray fluorescence imaging (XFI). Here, small animals are raster scanned with a pencil-like synchrotron beam of high energy and low divergence and the X-ray fluorescence is recorded with an energy-dispersive detector. The ability to first perform coarse scans to identify regions of interest, followed by a close-up with a sub-mm X-ray beam is desirable, because overall measurement time and X-ray dose absorbed by the (biological) specimen can thus be minimized. However, the size of X-ray beams at synchrotron beamlines is usually strongly dependent on the actual beamline setup and can only be adapted within specific pre-defined limits. Especially, large synchrotron beams are non-trivial to generate. Here, we present the concept of graphite-based, convex reflection optics for the one-dimensional enlargement of a 1 mm wide synchrotron beam by a factor of 5 to 10 within a 1 m distance. Four different optics are tested and characterized and their reflection properties compared to ray tracing simulations. The general shape and size of the measured reflection profiles agree with expectations. Enhancements with respect to homogeneity and efficiency can be expected with improved optics manufacturing. A mouse phantom is used for a proof-of-principle XFI experiment demonstrating the applicability of coarse and fine scans with the suggested optics design.     

Effects of Long-Term Temozolomide Treatment on Glioblastoma and Astrocytoma WHO Grade 4 Stem-like Cells

Glioblastoma leads to a fatal course within two years in more than two thirds of patients. An essential cornerstone of therapy is chemotherapy with temozolomide (TMZ). The effect of TMZ is counteracted by the cellular repair enzyme O⁶-methylguanine-DNA methyltransferase (MGMT). The MGMT promoter methylation, the main regulator of MGMT expression, can change from primary tumor to recurrence, and TMZ may play a significant role in this process. To identify the potential mechanisms involved, three primary stem-like cell lines (one astrocytoma with the mutation of the isocitrate dehydrogenase (IDH), CNS WHO grade 4 (HGA)), and two glioblastoma (IDH-wildtype, CNS WHO grade 4) were treated with TMZ. The MGMT promoter methylation, migration, proliferation, and TMZ-response of the tumor cells were examined at different time points. The strong effects of TMZ treatment on the MGMT methylated cells were observed. Furthermore, TMZ led to a loss of the MGMT promoter hypermethylation and induced migratory rather than proliferative behavior. Cells with the unmethylated MGMT promoter showed more aggressive behavior after treatment, while HGA cells reacted heterogenously. Our study provides further evidence to consider the potential adverse effects of TMZ chemotherapy and a rationale for investigating potential relationships between TMZ treatment and change in the MGMT promoter methylation during relapse.     

Formal Concept Analysis in knowledge processing: A survey on models and techniques

This is the first part of a large survey paper in which we analyze recent literature on Formal Concept Analysis (FCA) and some closely related disciplines using FCA. We collected 1072 papers published between 2003 and 2011 mentioning terms related to Formal Concept Analysis in the title, abstract and keywords. We developed a knowledge browsing environment to support our literature analysis process. We use the visualization capabilities of FCA to explore the literature, to discover and conceptually represent the main research topics in the FCA community. In this first part, we zoom in on and give an extensive overview of the papers published between 2003 and 2011 on developing FCA-based methods for knowledge processing. We also give an overview of the literature on FCA extensions such as pattern structures, logical concept analysis, relational concept analysis, power context families, fuzzy FCA, rough FCA, temporal and triadic concept analysis and discuss scalability issues.     

Wideband Low‐Reflection Transmission Lines for Bare Chip on Multilayer PCB

The pad pitch of modern radio frequency integrated circuits is in the order of few tens of micrometers. Connecting a large number of high‐speed I/Os to the outside world with good signal fidelity at low cost is an extremely challenging task. To cope with this requirement, we need reflection‐free transmission lines from an on‐chip pad to on‐board SMA connectors. Such a transmission line is very hard to design due to the difference in on‐chip and on‐board feature size and the requirement for extremely large bandwidth. In this paper, we propose the use of narrow tracks close to chip and wide tracks away from the chip. This narrow‐to‐wide transition in width results in impedance discontinuity. A step change in substrate thickness is utilized to cancel the effect of the width discontinuity, thus achieving a reflection‐free microstrip. To verify the concept, several microstrips were designed on multilayer FR4 PCB without any additional manufacturing steps. The TDR measurements reveal that the impedance variation is less than 3 Ω for a 50 Ω microstrip and S11 better than –9 dB for the frequency range 1 GHz to 6 GHz when the width changes from 165 µm to 940 µm, and substrate thickness changes from 100 µm to 500 µm.