Hydrogen production and metabolic flux analysis of metabolically engineered Escherichia coli strains

Escherichia coli can produce H₂ from glucose via formate hydrogen lyase (FHL). In order to improve the H₂ production rate and yield, metabolically engineered E. coli strains, which included pathway alterations in their H₂ production and central carbon metabolism, were developed and characterized by batch experiments and metabolic flux analysis. Deletion of hycA, a negative regulator for FHL, resulted in twofold increase of FHL activity. Deletion of two uptake hydrogenases (1 (hya) and hydrogenase 2 (hyb)) increased H₂ production yield from 1.20 mol/mol glucose to 1.48 mol/mol glucose. Deletion of lactate dehydrogenase (ldhA) and fumarate reductase (frdAB) further improved the H₂ yield; 1.80 mol/mol glucose under high H₂ pressure or 2.11 mol/mol glucose under reduced H₂ pressure. Several batch experiments at varying concentrations of glucose (2.5–10 g/L) and yeast extract (0.3 or 3.0 g/L) were conducted for the strain containing all these genetic alternations, and their carbon and energy balances were analyzed. The metabolic flux analysis revealed that deletion of ldhA and frdABdirected most of the carbons from glucose to the glycolytic pathway leading to H₂ production by FHL, not to the pentose phosphate pathway.     

Comparison of steam and autothermal reforming of methanol using a packed-bed low-cost copper catalyst

Small-scale reformers for hydrogen production via steam and autothermal reforming of hydrocarbon feedstocks can be a solution to the lack of hydrogen distribution infrastructure. A packed-bed reactor is one possible design for such purpose. However, the two reforming processes of steam and autothermal methods have different characteristics, thus they have different and often opposite design requirements. In implementing control strategy for small-scale reformers, understanding the overall chemical reactions and the reactor physical properties becomes essential. This paper presents some inherent features of a packed-bed reactor that can both improve and/or degrade the performance of a packed-bed reactor with both reforming modes.The high thermal resistance of the packed bed is disadvantageous to steam reforming (SR), but it is beneficial to the autothermal reforming (ATR) mode with appropriate reactor geometry. The low catalyst utilization in steam reforming can help to prevent the unconverted fuel leaving the reactor during transient by allowing briefly for higher reactant fuel flow rates. In this study, experiments were performed using three reactor geometries to illustrate these properties and a discussion is presented on how to take advantages of these properties in reactor design.     

Two-Section Cascaded Coupled Line Wilkinson Power Divider for Dual-Band Applications

A coupled line based design method is presented for the dual-band operation of the Wilkinson power divider. It is shown that a simple two-section cascaded coupled line structure is capable of the dual-band operation in the Wilkinson power divider. The overall divider structure can be simplified with the small number of transmission line sections and the coupled line using the proposed approach.     

Capacity analysis for distributed antenna systems using cooperative transmission schemes in fading channels

Wireless systems equipped with distributed antennas can provide enhanced data throughput as well as improved signal quality when appropriate cooperative transmission schemes among antennas are employed. In this paper, the performance of distributed antenna systems (DAS) under the influence of Rayleigh fading and log-normal shadowing effects is investigated for different cooperation strategies, and corresponding statistical distributions for both the signal-to-interference ratio (SIR) and the capacity are presented. Specifically, the ergodic capacity per remote antenna (RA) is determined when n RAs cooperatively transmit the desired signal to the target receiver, which is compared with the non-cooperative single-antenna transmission (SAT) case. Using the results, a preferable mode of operation for given geographic locations of the receiver is determined. We further propose to perform an adaptive operation between the cooperative and non-cooperative modes under the capacity maximization criteria, and quantify exact amounts of capacity gain over individual transmission modes.     

Environmental and operational stability of solution-processed 6,13-bis(triisopropyl-silylethynyl) pentacene thin film transistors

We report operational and environmental stability of solution-processed organic thin film transistors (OTFTs) using the small molecule organic semiconductor 6,13-bis(triisopropyl-silylethynyl) pentacene (TIPS-pentacene). Typical drop-cast TIPS-pentacene OTFTs show strong molecular ordering and relatively stable characteristics with air and illumination compared to vapor-deposited pentacene OTFTs. For short channel length devices, TIPS-pentacene OTFTs undergo significant degradation with bias-stress, possibly due to operation at large drive currents and large local power dissipation as well as built-in charges in the channel area.     

Electro-conductive properties of poly(3,4-ethylenedioxythiophene)/poly(ionic liquid) films with respect to its structure and morphology

Poly(3,4-ethylenedioxythiophene) (PEDOT)/poly(1-vinyl-3-ethylimidazolium⁺ (trifluoromethane sulfonyl)imide^?) (PIL) complexes were prepared at various PEDOT/PIL molar ratios and dispersed in propylene carbonate at a concentration of 1 wt%. After casting, the maximum conductivity was measured to be 1.2 × 10^?1 S/cm, which could be explained by the 3D variable range hopping model. The optimum surface roughness of the PEDOT/PIL film was measured, showing S_a and S_q values of 5.92 and 11.0 nm, respectively. The conductivity of the polymerized PEDOT without a template process had low conductivity due to its poor surface roughness and large particle size. Therefore, the conductivity of PEDOT/PILs is determined by the particle size, crystallinity and surface morphology. These results were supported by surface mapping microscopy, X-ray photon spectroscopy, and X-ray diffraction.     

Thermal crown analysis of the roll in the strip casting process

In the twin roll strip casting process, coupled analyses of heat transfer and deformation for the casting roll are carried out by using the finite element program MARC to examine the thermal crown. The shrink fit effect and plastic deformation are considered. The result shows that the thermal crown is greatly influenced by the shrink fit and that the thermal crown for POSCO Pilot Caster 2 Copper Roll has an “M” shape. The effects of several factors on thermal crown are also investigated. The amount of thermal crown increases as the heat flux, casting speed, sleeve thickness and casting roll width increase. On the other hand, it decreases as the casting roll diameter increases.     

Effect of hydrostatic pressure on the decomposition behavior of Cu54Zr22Ti18Ni6 bulk metallic glass alloy in supercooled liquid region

The effect that hydrostatic pressure has on the decomposition behavior in Cu₅₄Ni₆Zr₂₂Ti₁₈ bulk metallic glass (BMG) alloy was assessed using samples, which were isothermally annealed in the supercooled liquid region (SLR) with and without application of hydrostatic pressure. During subsequent annealing for the thermal analysis, the samples annealed under hydrostatic pressure displayed a slower crystallization process in the SLR, which is attributed to a retarded compositional decomposition of the BMG alloy under pressure.     

High rate capability of carbonaceous composites as anode electrodes for lithium-ion secondary battery

Anode materials with high rate capability for Li-ion secondary batteries were investigated by using the mixture of graphite, cokes, and petroleum pitch. Since obvious potential plateaus were obtained at graphite contents above 40 wt.%, which would cause difficulties in perceiving the capacity variations as a function of electrical potential, the graphite content were determined at 20–30 wt.%. The composites with a given content of graphite and remaining content of petroleum pitch/cokes mixtures at 1:4, 1:1, and 4:1 mass ratios were heated at a temperature range of 800–1200 °C. For a given composition of carbonaceous composite, the discharge rate capability improved but the reversible capacity decreased with increasing the heat treatment temperature. Although the reversible capacity increased with increasing content of the petroleum pitch for given graphite content and heat treatment temperature, the discharge rate capability decreased. The carbonaceous composites prepared by the mixture of 30 wt.% graphite and 70 wt.% petroleum pitch/cokes mixture at 1:4 mass ratio with the heat treatment at 800 °C showed relatively high electrochemical properties, of which reversible capacity, initial efficiency, discharge rate capability (retention of discharge capacity in 5 C/0.2 C) and charge capacity at 5 C were 312 mAh/g, 79%, 89% and 78 mAh/g, respectively.     

Effect of water drying conditions on the surface property and morphology of waterborne UV-curable coatings for engineered flooring

UV-curable coatings are widely used on wooden materials such as flooring and furniture, because they have excellent properties, including high hardness, gloss, mar and chemical resistance, and are also environmental friendly, containing no solvents. Recently, waterborne UV-curable coatings have been studied as a viable alternative, since solvents are added to spray applications to lower viscosity. We investigated the effects of water drying conditions on the surface properties and morphology of waterborne UV-curable coatings at the flash-off step. Temperature conditions studied were 22 °C, 50 °C, and 80 °C, with various drying times. We evaluated surface properties such as pendulum hardness, pencil hardness, and adhesion strength. Also observed was surface morphology, comparing surface properties using optical microscopy after drying and UV-curing. Insufficient drying caused cracking, peeling and blistering at the surface of a cured coating. Sufficient drying was very important for the best application and ideal surface morphology of waterborne UV-curable coatings.