用于腺苷检测的基于级联扩增核酸适体电化学传感界面的构建研究

该文报道了一种基于酶级联扩增的电化学核酸适体检测腺苷的新方法。当腺苷存在时,滚环扩增的引物通过E.coli DNA连接酶的作用与通过巯基组装在金电极上的固定探针连接。在pHi29 DNA聚合酶的作用下,滚环扩增反应进行并产生一条与环形探针完全互补的长的单链,然后将大量金纳米粒子标记的核酸探针与滚环扩增的产物杂交。该传感界面电化学行为的结果表明,通过酶级联扩增的方法提高了检测腺苷的阻抗响应灵敏度,且选择性和重现性良好。用于腺苷的检测时,其线性范围为2.0μmol/L~100μmol/L,最低检测浓度为2.0μmol/L,表明该传感界面的设计可作为一种通用方法而有望用于其它目标物的检测。     

Optimizing content placement in a peer-assisted VoD architecture

Recently, a new peer-assisted architecture to build content delivery systems has been presented. This architecture is based on the use of the storage capacity of end-users’ set-top boxes (STBs), connected in a peer-to-peer (P2P) manner in order to help the content servers in the delivery process. In these systems, the contents are usually split into a set of smaller pieces, called sub-streams, which are randomly injected at the STBs. The present paper is focused on Video on Demand (VoD) streaming and it is assumed that the STB-based content delivery system is deployed over the global Internet, where the clients are distributed over different ISP networks. In this scenario, three different strategies are studied for increasing the percentage of data uploaded by peers, in order to offload the content servers as much as possible. First of all, a new mechanism is presented which determines which sub-stream has to be placed at which STB by a Non-Linear Programming (NLP) formulation. A different strategy for reducing the content server load is to take advantage of the available bandwidth in the different ISP networks. In this sense, two new mechanisms for forwarding the VoD requests to different ISP networks are presented. Finally, the present paper also shows that in some situations the available uplink bandwidth is associated with STBs that do not have the required sub-streams. Regarding this concern, a new mechanism has been designed that dynamically re-allocates some streams, which are being transmitted from specific STBs, to different STBs, in order to find the necessary resources to start new streaming sessions.     

A kernel-based framework for image collection exploration

While search engines have been a successful tool to search text information, image search systems still face challenges. The keyword-based query paradigm used to search in image collection systems, which has been successful in text retrieval, may not be useful in scenarios where the user does not have the precise way to express a visual query. Image collection exploration is a new paradigm where users interact with the image collection to discover useful and relevant pictures. This paper proposes a framework for the construction of an image collection exploration system based on kernel methods, which offers a mathematically strong basis to address each stage of an image collection exploration system: image representation, summarization, visualization and interaction. In particular, our approach emphasizes a semantic representation of images using kernel functions, which can be seamlessly harnessed across all system components. Experiments were conducted with real users to verify the effectiveness and efficiency of the proposed strategy.     

独立学院VFP程序设计教学存在的问题及解决方法

根据独立学院的学生特征和VFP课程的教学现状,以提高学生的计算机应用能力为目的,提出摒弃传统教学"重理论,轻实践"的思想,采用案例教学法和任务驱动法,在真实情境中让学生自己动手由浅入深,逐步开发出一个完整的管理信息系统.     

浅析电子商务中的安全问题

随着现今社会互联网、个人终端技术的飞越发展,电子商务越来越多的出现在人们的生活与工作中,这种全新的商务模式具有便捷、高效率、低成本的特点.同时,这种全新的商务模式对管理水平、信息传递技术都提出了更高的要求,其中安全的重要性尤为突出.     

计算机网络安全及防范策略探究

随着计算机科学技术的发展,计算机网络已广泛用于经济、军事、教育等各个领域。因此,计算机的网络安全便成为涉及个人、单位、社会、国家信息安全的重大问题。如何保障网络信息的安全已成为当前人们迫在眉睫需要解决的问题。本文就计算机网络安全技术及相关策略提出了一点粗浅的看法。     

电力监控系统的跨平台解决方案

随着应用的发展,电力监控系统越来越大型化,跨平台的应用越来越多.本文阐述了采用分层归纳的设计思想,归纳电力系统数据处理的逻辑应用层,包装与具体操作系统密切相关的操作系统层,提炼与具体操作系统进程机制有关的程序框架,来实现电力监控软件系统的跨平台应用.并以C语言为主要编程语言进行了电力监控系统跨平台实现,结果表明该方法能够跨越Windows、Linux、Solaris等平台,达到源代码级的跨平台效果.     

卷草纹的艺术语言及其现代延展设计

卷草纹是中国传统装饰纹样之一,它的形成和发展记载了中国装饰艺术旺盛的生命力。本文分析了卷草纹的发展过程、形象构成以及艺术特征,并通过具体案例诠释了它在现代设计各个领域的延展和应用。旨在探索传统装饰纹样在现代设计中的传承与发展的方向。     

基于J2EE的地税网上申报系统的设计与实现

文章阐述了地税网上申报系统开发的背景和意义;介绍了基于J2EE的网上报税系统的结构、软件的主要功能及具体的实现方法和所用的关键技术;总结了所用技术的优势以及系统中难点的处理。     

Stabilizing Redox-Active Hexaazatriphenylene in a 2D Conductive Metal–Organic Framework for Improved Lithium Storage Performance

Organic redox-active materials are promising electrode candidates for lithium-ion batteries by virtue of their designable structure and cost-effectiveness. However, their poor electrical conductivity and high solubility in organic electrolytes limit the device's performance and practical applications. Herein, the π-conjugated nitrogen-containing heteroaromatic molecule hexaazatriphenylene (HATN) is strategically embedded with redox-active centers in the skeleton of a Cu-based 2D conductive metal–organic framework (2D c-MOF) to optimize the lithium (Li) storage performance of organic electrodes, which delivers improved specific capacity (763 mAh g⁻¹ at 300 mA g⁻¹), long-term cycling stability (≈90% capacity retention after 600 cycles at 300 mA g⁻¹), and excellent rate performance. The correlation of experimental and computational results confirms that this high Li storage performance derives from the maximum number of active sites (CN sites in the HATN unit and CO sites in the CuO₄ unit), favorable electrical conductivity, and efficient mass transfer channels. This strategy of integrating multiple redox-active moieties into the 2D c-MOF opens up a new avenue for the design of high-performance electrode materials.