Dynamic stress relaxation of thermoplastic elastomeric biomaterials

In this paper we present the results of comparative dynamic stress relaxation studies performed with poly(styrene-b-isobutylene-b-styrene) (SIBS), polyurethane (PU) and polyester (PED) biomaterials in air and simulated body fluid (SBF) at 24 °C and 37 °C. SIBS showed the highest value of relieved stress under constant strain (24.1% after 100,000 cycles in air) with PED and PU having similar relative change (12.2% and 10.5%). In spite of its softness (Shore A 56 vs. 80), the dynamic modulus (E_dyn) and stiffness of SIBS were in between PED and PU. The behavior of the materials was correlated to their structure: SIBS is an amorphous block copolymer with a long elastomer midblock, while PU and PED are semicrystalline segmented copolymers with much shorter soft blocks, and hydrogen bonding. SIBS and PED were relatively insensitive to SBF and temperature changes, while PU experienced the largest changes in physical properties in vitro (simulated body fluid, 37 °C).     

Keeping uracil out of DNA: physiological role, structure and catalytic mechanism of dUTPases

The thymine-uracil exchange constitutes one of the major chemical differences between DNA and RNA. Although these two bases form the same Watson-Crick base pairs with adenine and are equivalent for both information storage and transmission, uracil incorporation in DNA is usually a mistake that needs to be excised. There are two ways for uracil to appear in DNA: thymine replacement and cytosine deamination. Most DNA polymerases readily incorporate dUMP as well as dTMP depending solely on the availability of the d(U/T)TP building block nucleotides. Cytosine deamination results in mutagenic U:G mismatches that must be excised. The repair system, however, also excises U from U:A "normal" pairs. It is therefore crucial to limit thymine-replacing uracils.dUTP is constantly produced in the pyrimidine biosynthesis network. To prevent uracil incorporation into DNA, representatives of the dUTP nucleotidohydrolase (dUTPase) enzyme family eliminate excess dUTP. This Account describes recent studies that have provided important detailed insights into the structure and function of these essential enzymes.dUTPases typically possess exquisite specificity and display an intriguing homotrimer active site architecture. Conserved residues from all three monomers contribute to each of the three active sites within the dUTPase. Although even dUTPases from evolutionarily distant species possess similar structural and functional traits, in a few cases, a monomer dUTPase mimics the trimer structure through an unusual folding pattern. Catalysis proceeds by way of an SN2 mechanism; a water molecule initiates in-line nucleophilic attack. The dUTPase binding pocket is highly specific for uracil. Phosphate chain coordination involves Mg2+ and is analogous to that of DNA polymerases. Because of conformational changes in the enzyme during catalysis, most crystal structures have not resolved the residues in the C-terminus. However, recent high-resolution structures are beginning to provide in-depth structural information about this region of the protein.The dUTPase family of enzymes also shows promise as novel targets for anticancer and antimicrobial therapies. dUTPase is upregulated in human tumor cells. In addition, dUTPase inhibitors could also fight infectious diseases such as malaria and tuberculosis. In these respective pathogens, Plasmodium falciparum and Mycobacterium tuberculosis, the biosynthesis of dTMP relies exclusively on dUTPase activity.     

The main features of suburbanization in the Great Hungarian Plain

Suburbanization, which took place through different mechanisms, and occured earlier in western European countries, is a relatively new phenomenon in Hungary. The Great Plain was only affected by industrial decentralization in the 1960s.The housing shortage is a serious problem in Hungary, thus the cheaper sites for sale in the suburds are especially attractive. Moreover, demands for space in the Great Plain compared with other regions of the country are greater here for people used to living in the large agrarian towns. The fact that they can do household and supplementary farming around their homes is an important incentive. This only means self-provision for many of them, but others carry out market gardening. Agriculture is the only survival strategy for numbers of people in the Great Plain, given the present dismal state of the Hungarian economy. Therefore, suburbs here are of unmistakably rural character and their population is not homogenous. Infrastructural investment has not kept up with the increase in population. Very often it is the commuting villages which have turned into suburbs, and with spatial relationships becoming more intense, they generally lose their administrative independence.     

Ventilation system design for a church pipe organ using numerical simulation and on-site measurement

Since old churches have had heating installed, more complaints have arisen of organs sounding out of tune. Sound frequency of organ pipes is dependent on air temperature. Old churches tend to have very large volumes, so are typically heated just before and during services in wintertime, in order to reduce energy usage. Warm air plumes rise at radiators and spread out into the room, finally reaching the cold organ casing where they cause a non-uniform temperature distribution within. If pipes standing in different temperature zones are played at the same time the organ sounds out of tune due to clearly audible beats (interference between two slightly different frequencies). The purpose of this study was to design a ventilation system inside the organ casing, able to create a uniform temperature distribution around the pipes. A computational fluid dynamic (CFD) model was developed for the St. Martin church in Oberesslingen, Germany. It was validated by on-site measurements that had been carried out in the organ casing of the church. Four organ divisions containing 300–500 pipes were represented by equivalent porous material zones. Their properties were determined using an auxiliary two-dimensional model of a staggered array consisting of 392 pipes. The effect of different ventilation system settings on the temperature field in the organ casing was examined. Best results were achieved by a system consisting of two ventilation lines together with a thin, synthetic net placed at the open interface between church and organ casing in order to reduce warm air inflow.