One-pot synthesis of gelatin-based,slow-release polymer microparticles containing silver nanoparticles and their application in anti-fouling paint

Gelatinous polymer matrix microparticles containing silver nanoparticles (AgNPs) were prepared by a novel method to obtain quasi non-swelling anti-fouling paint additives with slow-release characteristics. A w/o type dispersion were elaborated with the aqueous phase of gelatin, urea, silver-nitrate and formaldehyde dispersed in linseed oil. Gelatin was cross-linked by formaldehyde, together with urea for limiting the swelling of the product. Silver-nitrate was reduced with the assistance of gelatin and formaldehyde into homogenously dispersed AgNPs. The microparticles and embedded AgNPs were visualized by scanning and transmission electronmicroscopy. Encapsulated AgNPs with ∼18 nm crystallite size were identified by X-ray powder diffraction. Characterization of gelatin–urea–formaldehyde polymer matrices was carried out by attenuated total reflectance FTIR spectroscopy. Silver dissolution from microparticles and paints with AgNP-containing microparticles was measured by inductively coupled plasma spectrometer and resulted in highly sustained release, compared to unmodified gelatin microparticles and paints containing uncapsulated silver salts. A 7-month-long fouling experiment run in natural sweetwater media showed that solvent-based acrylic paint with AgNPs-containing gelatinous microparticles as additives offered resistance against biofouling at low Ag-release ratio.     

Using Dynamic Information in the Interprocedural Static Slicing of Binary Executables

Although the slicing of programs written in a high-level language has been widely studied in the literature, relatively few papers have been published on the slicing of binary executable programs. The lack of existing solutions for the latter is really hard to understand since the application domain for slicing binaries is similar to that for slicing high-level languages. Furthermore, there are special applications of the slicing of programs without source code like source code recovery, code transformation and the detection of security critical code fragments. In this paper, in addition to describing the method of interprocedural static slicing of binaries, we discuss how the set of the possible targets of indirect call sites can be reduced by dynamically gathered information. Our evaluation of the slicing method shows that, if indirect function calls are extensively used, both the number of edges in the call graph and the size of the slices can be significantly reduced.Ákos Kiss obtained his M.Sc. in Computer Science from the University of Szeged in 2000. He is currently working on his Ph.D. thesis and his chosen field of research is the analysis and optimization of binary executables. He was the chief programmer of a code compaction project which sought to reduce ARM binaries. He is also interested in GCC and in open source developmentJudit Jász obtained her M.Sc. in Computer Science recently from the University of Szeged and is currently a Ph.D student. Her main research interest is adapting slicing methods—originally intended for high-level languages—to binary executables. She is also actively working on improving the GCC compiler.Tibor Gyimóthy is the head of the Software Engineering Department at the University of Szeged in Hungary. His research interests include program comprehension, slicing, reverse engineering and compiler optimization. He has published over 60 papers in these areas and was the leader of several software engineering R&D projects. He is the Program Co-Chair of the 21th International Conference on Software Maintenance, which will be held in Budapest, Hungary in 2005.     

A Matrix-Based Approach to the Image Moment Problem

An image can be seen as an element of a vector space and hence it can be expressed in as a linear combination of the elements of any non necessarily orthogonal basis of this space. After giving a matrix formulation of this well-known fact, this paper presents a reconstruction method of an image from its moments that sheds new light on this inverse problem. Two main contributions are presented: (a) the results using the standard approach based on the least squares approximation of the result using orthogonal polynomials can also be obtained using matrix pseudoinverses, which implies higher control on the numerical stability of the problem; and (b) it is possible to use basis functions in the reconstruction different from orthogonal polynomials, such as Fourier or Haar basis, allowing to introduce constraints relative to the bandwidth or the spatial resolution on the image to be reconstructed. Judit Martònez received the B.Sc. degree in 1993 and the PhD degree (with honors) in 1998, both in telecommunications engineering from the Technical University of Catalonia. She developed her research at the Institut de Robútica i Informütica Industrial of the Spanish High Council for Scientific Research. In 1999 she joined the Computer Vision Center, a R&D center founded by the Autonomous University of Barcelona and the Autonomous Government of Catalonia. She has been principal researcher of several industrial and research projects related to computer vision technologies. Her research interests include industrial applications of machine vision, efficient algorithms for low-level image processing, multiresolution mathematical models, statistical clustering, pattern classification and inverse problems. Josep M. Porta received the Engineer Degree in Computer Science in 1994 and the Ph.D. in Artificial Intelligence in 2001, both from the Technical University of Catalonia (UPC). After that, he joined the IAS group of the University of Amsterdam and currently, he holds a post-doc position at the Institut de Robútica i Informütica Industrial (CSIC-UPC) in Barcelona. He carried research in legged robots, machine learning, vision-based methods for autonomous robot localization, and computational kinematics. Federico Thomas is Research Professor at the Spanish Scientific Research Council (CSIC) and director of the Institut de Robútica i Informütica Industrial (CSIC-UPC), Barcelona, Spain. He received the telecommunications engineering degree in 1984, and the Ph.D. degree (with honors) in computer science in 1988, both from the Technical University of Catalonia (UPC). In 1991, he won a NATO postdoctoral scholarship at the University of Massachusetts with the late Prof. Robin Popplestone. In 1999, he was visiting professor, sponsored by the Autonomous Government of Catalonia, at the Oxford University Computing Laboratory with Prof. Stephen Cameron. He has been project leader of several national projects financed by the Spanish Committee for Science and Technology (CICYT), and by local companies such as ENHER, a power generation company now part of ENDESA. His current research interests are in Geometry and Kinematics with applications to Robotics, Computer Graphics and Computer Vision. Prof. Thomas is an Associate Editor of the IEEE Transactions on Robotics.     

Personal RF exposimetry in urban area

The purpose of the current study was to evaluate the usefulness of a radiofrequency (RF) personal exposimeter (dosimeter) for assessing individual RF exposure in an urban environment. Measurements taken by RF personal dosimeter were also compared to preliminary site measurements taken around mobile base stations. The results from personal exposure showed that one third of the participants spent 40–70% of 24 h recording time above the detection limits (0.05 V/m), and half of subjects spent less than 10%. The highest exposure was detected during the traveling period and the lowest in bed at home. Based on our results, we concluded that site measurements cannot be used to accurately determine personal exposure. We also concluded that duration of time exposed to RF levels above the detection limit of the personal dosimeter is a useful exposure metric to compare and contrast individual RF exposure of study subjects.     

Preparation and characterization of hydroxyapatite from eggshell

Hydroxyapatite (HAp) was successfully produced by using recycled eggshell. The observed phases of the synthesised materials were dependent on the mechanochemical activation method (ball milling and attrition milling). The structures of the HAp were characterized by X-ray diffraction, scanning electron microscopy and infrared spectroscopy. Attrition milling proved to be more efficient than ball milling, as resulted nanosize, homogenous HAp even after milling.     

PET Probes for Preclinical Imaging of GRPR-Positive Prostate Cancer: Comparative Preclinical Study of [68Ga]Ga-NODAGA-AMBA and [44Sc]Sc-NODAGA-AMBA

Gastrin-releasing peptide receptors (GRPR) are overexpressed in prostate cancer (PCa). Since bombesin analogue aminobenzoic-acid (AMBA) binds to GRPR with high affinity, scandium-44 conjugated AMBA is a promising radiotracer in the PET diagnostics of GRPR positive tumors. Herein, the GRPR specificity of the newly synthetized [⁴⁴Sc]Sc-NODAGA-AMBA was investigated in vitro and in vivo applying PCa PC-3 xenograft. After the in-vitro assessment of receptor binding, PC-3 tumor-bearing mice were injected with [⁴⁴Sc]Sc/[⁶⁸Ga]Ga-NODAGA-AMBA (in blocking studies with bombesin) and in-vivo PET examinations were performed to determine the radiotracer uptake in standardized uptake values (SUV). ⁴⁴Sc/⁶⁸Ga-labelled NODAGA-AMBA was produced with high molar activity (approx. 20 GBq/µmoL) and excellent radiochemical purity. The in-vitro accumulation of [⁴⁴Sc]Sc-NODAGA-AMBA in PC-3 cells was approximately 25-fold higher than that of the control HaCaT cells. Relatively higher uptake was found in vitro, ex vivo, and in vivo in the same tumor with the ⁴⁴Sc-labelled probe compared to [⁶⁸Ga]Ga-NODAGA-AMBA. The GRPR specificity of [⁴⁴Sc]Sc-NODAGA-AMBA was confirmed by significantly (p ≤ 0.01) decreased %ID and SUV values in PC-3 tumors after bombesin pretreatment. The outstanding binding properties of the novel [⁴⁴Sc]Sc-NODAGA-AMBA to GRPR outlines its potential to be a valuable radiotracer in the imaging of GRPR-positive PCa.     

Metabolic Adaptation as Potential Target in Papillary Renal Cell Carcinomas Based on Their In Situ Metabolic Characteristics

Metabolic characteristics of kidney cancers have mainly been obtained from the most frequent clear cell renal cell carcinoma (CCRCC) studies. Moreover, the bioenergetic perturbances that affect metabolic adaptation possibilities of papillary renal cell carcinoma (PRCC) have not yet been detailed. Therefore, our study aimed to analyze the in situ metabolic features of PRCC vs. CCRCC tissues and compared the metabolic characteristics of PRCC, CCRCC, and normal tubular epithelial cell lines. The protein and mRNA expressions of the molecular elements in mammalian target of rapamycin (mTOR) and additional metabolic pathways were analyzed in human PRCC cases compared to CCRCC. The metabolic protein expression pattern, metabolite content, mTOR, and metabolic inhibitor sensitivity of renal carcinoma cell lines were also studied and compared with tubular epithelial cells, as “normal” control. We observed higher protein expressions of the “alternative bioenergetic pathway” elements, in correlation with the possible higher glutamine and acetate consumption in PRCC cells instead of higher glycolytic and mTOR activity in CCRCCs. Increased expression of certain metabolic pathway markers correlates with the detected differences in metabolite ratios, as well. The lower lactate/pyruvate, lactate/malate, and higher pyruvate/citrate intracellular metabolite ratios in PRCC compared to CCRCC cell lines suggest that ACHN (PRCC) have lower Warburg glycolytic capacity, less pronounced pyruvate to lactate producing activity and shifted OXPHOS phenotype. However, both studied renal carcinoma cell lines showed higher mTOR activity than tubular epithelial cells cultured in vitro, the metabolite ratio, the enzyme expression profiles, and the higher mitochondrial content also suggest increased importance of mitochondrial functions, including mitochondrial OXPHOS in PRCCs. Additionally, PRCC cells showed significant mTOR inhibitor sensitivity and the used metabolic inhibitors increased the effect of rapamycin in combined treatments. Our study revealed in situ metabolic differences in mTOR and metabolic protein expression patterns of human PRCC and CCRCC tissues as well as in cell lines. These underline the importance in the development of specific new treatment strategies, new mTOR inhibitors, and other anti-metabolic drug combinations in PRCC therapy.     

Einfluss der Lufttemperatur auf die Verstimmung von Orgelpfeifen

Influence of air temperature on detuning of organ pipes. During winter a lot of pipe organs sound out‐of‐tune. Before playing the organ, the church is heated quite fast. In the organ case different temperature zones arise, and apart from that organ pipes standing in already warm environment are supplied with cold air by the windsystem. In order to examine the influence of blowing cold air into a pipe on its pitch, a model organ was set up in a two room climate chamber at the Fraunhofer‐Institute for Building Physics. Blower and bellows were located in the cold part of the chamber, the windchest with testing pipe was arranged in the warm part. Temperature transients at five different positions in the pipe resonator were measured, the pipe sound was recorded at the same time and finally the fundamental frequency was determined. On several positions in the windsystem temperature sensors were set in order to detect local temperature transients. These experiments were part of a project for the development of climate systems applicable for pipe organs with the purpose of creating a constant temperature distribution in organ cases and inside of windsystems. The detuning of organs and mold growth should be decreased.     

Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina

The purple (Sulphur) phototrophic bacterium, Thiocapsa roseopersicina BBS contains several [NiFe] hydrogenases, of which two are membrane bound. Mutant T. roseopersicina cells, carrying deletions in both gene clusters showed hydrogenase activity. This activity was located in the cytoplasm. The structural gene cluster hoxEFUYH was identified and sequenced. In addition, genes homologous to hupUV/hoxBC, the hydrogen sensing hydrogenase have been identified and sequenced.Regulation of hydrogenase biosynthesis was studied in detail for HydSL (renamed HynSL). A random mutagenesis system was optimised for T. roseopersicina. One of the mutations was in a gene similar to that coding for the HypF proteins in other organisms. Inactivation of the hypF gene resulted in a 60-fold increase in hydrogen evolution under nitrogen fixing conditions. In addition to hypF, the following accessory genes were identified: hydD, hupK, hypC1, hypC2, hypDE. Characterisation of the corresponding gene products and search for additional accessory genes are in progress.