Determination of the Henry's Coefficient and Mass Transfer for Ozone in a Bubble Column at Different pH Values of Water

In this study, the ozone gas-liquid mass transfer into water in a bubble column was investigated for different pH values. The ozone volumetric mass transfer coefficient and the Henry's coefficient were determined simultaneously by parameter estimation using a nonlinear optimization method. A sensitivity analysis was conducted to obtain information on the reliability and identifiability of the estimated parameters. A minor dependence of the Henry's law constant on pH was detected at the pH range 4 to 9.     

A workbench for distributed production management systems

An end-user oriented workbench approach in the design of distributed production management systems (DPMS) is presented. We shall also deal with models and design of DPMS. Changes anticipated in design processes, roles and professionality required are discussed. In fact, complete structural rearrangements in the design of production management systems (PMS) are expected towards the end of this decade. The ideas presented are based on an on-going research project at Helsinki University of Technology (the so-called HUTDPMS project).     

Shape recognition by a scale-invariant model

In this article, we considered the recognition of unknown shapes by maximum likelihood methods. The contour of a shape is represented by its centroidal profile, and it is fitted by a circular autoregressive model. Two different shape recognition problems are considered: the decision on the similarity of two unknown shapes, and the classification of an unknown shape as one of many known shapes. Maximum likelihood decision rules for these two cases are derived. The decision rules are invariant to translation, rotation, and size change after normalizing the estimates. The developed algorithms are applied to classify eight classes of machine parts and eight classes of aircraft shapes. For each class, 60 to 80 samples are generated by rotating and dilating the original shape. In the experiment, more than 98% of machine parts are classified correctly, and more than 97% of aircraft shapes are correctly classified. This result is better than previous model-based approaches. Partially supported by the National Science Foundation under the grant IRI-8809391.     

Structural features of a polypeptide carrier promoting secretion of a β-lactamase fusion protein in yeast

Escherichia coli β-lactamase was secreted into the culture medium of Saccharomyces cerevisiae in biologically active form, when fused to the C-terminus of the hsp150δ-carrier. The hsp150δ-carrier is an N-terminal fragment of the yeast hsp150 protein, having a signal peptide and consisting mostly of a 19 amino acid peptide repeated 11 times in tandem. Here we expressed the hsp150δ-carrier fragment alone in S. cerevisiae. Apparently due to a positional effect of the gene insertion, large amounts of the hsp150δ-carrier were synthesized. About half of the de novo synthesized carrier molecules were secreted into the culture medium, the rest remaining mostly in the pre-Golgi compartment. The extensively O-glycosylated carrier fragment was purified from the culture medium under non-denaturing conditions. Circular dichroism spectroscopy showed that it had no regular secondary structure. Nuclear magnetic resonance spectroscopy showed that a non-glycosylated synthetic peptide, the consensus sequence of the repetitive 19 amino acid peptide, also lacked secondary structure. The unstructured carrier polypeptide may facilitate proper folding and secretion of heterologous proteins attached to it.     

Application of a novel method for the comparison of DNA binding parameters of the two human thyroid hormone receptor subtypes hTR alpha 1 and hTR beta 1

DNA-binding characteristics of the two human thyroid hormone receptors alpha 1 and beta 1 (hTR alpha 1 and hTR beta 1) were studied by applying the recently developed solid-phase scintillation technique. Biotinylated double stranded oligonucleotides containing thyroid hormone response elements (TRE) were immobilized to streptavidin coated scintillating microtiter plates. The TRE:s consisted of variants of the consensus core sequence AGGTCA as monomers or as dimers in direct repeats. Equilibrium binding of radioactive labelled hTR alpha 1 and hTR beta 1 were studied. Metabolically 35S-labelled hTR (in vitro translated cDNA) as well as hTR expressed in the baculovirus-system and labelled with 125I-triiodothyronine (125I-T3) were used. In binding saturation experiments, the affinity for the TRE:s investigated did not differ greatly between hTR alpha 1 and hTR beta 1. No significant effects of T3 on the amplitude of DNA binding of either hTR alpha 1 or hTR beta 1 to the single site response elements could be demonstrated. Receptor binding to direct repeats was stimulated by the hormone in the case of the hTR beta 1. The hTR alpha 1 binding to direct repeats was not significantly altered by T3. The single site octameric variant of a TRE, TAAGGTCA, was observed to bind tighter to the hTR:s as compared to the hexameric variant AGGTCA. In the binding competition format, with one response element immobilized and other (un-biotinylated) added to the reaction mixture, there was a larger dynamic range for the affinity constants (IC50) as compared to the affinity constants (Kd) obtained in the binding saturation experiments. The present quantitative results confirm previous reports obtained with qualitative methods like gel shift assays. The method described here is applicable in basic research concerning characterisation of DNA binding of nuclear receptors. It also lends itself to automatization in high capacity formats.     

On Design of Parallel Memory Access Schemes for Video Coding

Some of the modern powerful digital signal processors (DSPs) have byte-addressable internal data memory. This property is valuable especially in computationally demanding inter frame video encoding, where data accesses are typically unaligned according to word boundaries. The byte-addressable memory allows load or store command to start accessing from any byte-address, providing at most as many successive bytes from subsequent addresses as data bus can handle in parallel. Maybe the simplest way to construct such a byte-addressable memory is to use N 8-bit memory modules or banks to be accessed in parallel, when N is data bus width in bytes. However, in addition to byte-addressable subsequent bytes, memory consisting of parallel memory modules can provide much more versatile addressing capabilities with reasonable implementation cost. Versatile access formats can significantly reduce the need for data reordering in the register file. At first, we provide motivation for using parallel memory architecture with versatile access formats as an internal on-chip data memory of modern DSP. After this, notations are described and general view of parallel memory design is given. We propose some example parallel data memory architecture designs with data access formats especially helpful in H.263 encoding and MPEG-4 core profile motion and texture encoding. The examples are given for different data bus widths (16, 32, 64, and 128 bits). Finally, performance is shortly compared to other memory architectures and area, delay, and power figures are estimated.Jarno K. Tanskanen was born in Joensuu, Finland in 1975. He studied analog and digital electronics in the Department of Electrical Engineering, and computer architecture in the Department of Information Technology at Tampere University of Technology, where he received his M.Sc. degree in 1999. He is currently working as a research scientist in the Institute of Digital and Computer Systems at TUT. His Dr.Tech. research concerns parallel processing of video compression. jarno.tanskanen@tut.fiReiner Creutzburg received his Diploma in Mathematics in 1976 and attained his Ph.D. in Mathematics in 1984 from the Rostock University, Germany. Prof. Creutzburg has published 3 books, filed 2 patents, and produced approximately 100 articles, preprint, and conference papers. Professional Experience: Since 2000—Part-time Professor for Multimedia technology, Tampere University of Technology, Finland. Since 1992—Full-time Professor of Computer Science, Fachhochschule Brandenburg-University of Applied Sciences, Brandenburg, Germany. 1990 to 1992—Assistant Professor, University of Karlsruhe, Institute of Algorithms and Cognitive Systems, Germany. 1987 to 1989—Head of the Research Section Image Processing. 1986 to 1989—Founder and Head of the International Base Laboratory of Image Processing and Computer Graphics for East European countries at the Central Institute of Cybernetics and Information Processes of the Academy of Sciences (Berlin), Germany. 1976 to 1989—Researcher and Assistant Professor in various Universities and the Academy of Sciences, Central Institute of Cybernetics and Information, Berlin. creutzburg@fh-brandenburg.deJarkko T. Niittylahti was born in Orivesi, Finland, in 1962. He received the M.Sc, Lic.Tech, and Dr.Tech degrees at Tampere University of Technology (TUT) in 1988, 1992, and 1995, respectively. From 1987 to 1992, he was a researcher at TUT. In 1992–93, he was a researcher at CERN in Geneva, Switzerland. In 1993–95, he was with Nokia Consumer Electronics, Bochum, Germany, and in 1995–97 with Nokia Research Center, Tampere, Finland. In 1997–2000, he was a Professor at Signal Processing Laboratory, TUT, and in 2000–2002 at Institute of Digital and Computer Systems, TUT. Currently, he is a Docent of Digital Techniques at TUT and the managing director of Staselog Ltd. He is also a co-founder and President of Atostek Ltd. He is interested in designing digital systems and architectures. jarkko.niittylahti@tut.fi     

Heat treatment studies of a TLCP fiber

This work deals with the heat treatment of fibers and nonoriented specimens made of the thermotropic liquid crystalline polymer Vectra A900 in the light of tensile properties and DSC measurements. In calorimetric measurements, there seem to be some differences in the effects of the treatment between macroscopically isotropic (nonoriented) and highly oriented fiber specimens. Nonisothermal treatments also produce some effects on the tensile properties, which may be attributable to the thermal history of samples. Under the conditions of this study, a multistep treatment, ending at a final stage where the temperature was higher than the original melting point of the polymer, did not cause any acceleration in the rate at which tensile properties increase. © 1993 John Wiley & Sons, Inc.     

The core domain of retrotransposon integrase in Hordeum: predicted structure and evolution

Propagation of long terminal repeat (LTR)-bearing retrotransposons and retroviruses requires integrase (IN, EC 2.7.7.-), encoded by the retroelements themselves, which mediates the insertion of cDNA copies back into the genome. An active retrotransposon family, BARE-1, comprises approximately 7% of the barley (Hordeum vulgare subsp. vulgare) genome. We have generated models for the secondary and tertiary structure of BARE-1 IN and demonstrate their similarity to structures for human immunodeficiency virus 1 and avian sarcoma virus INs. The IN core domains were compared for 80 clones from 28 Hordeum accessions representative of the diversity of the genus. Based on the structural model, variations in the predicted, aligned translations from these clones would have minimal structural and functional effects on the encoded enzymes. This indicates that Hordeum retrotransposon IN has been under purifying selection to maintain a structure typical of retroviral INs. These represent the first such analyses for plant INs.     

Improving integrity of on-line grammage measurement with traceable basic calibration

The automatic control of grammage (basis weight) in paper and board production is based upon on-line grammage measurement. Furthermore, the automatic control of other quality variables such as moisture, ash content and coat weight, may rely on the grammage measurement. The integrity of Kr-85 based on-line grammage measurement systems was studied, by performing basic calibrations with traceably calibrated plastic reference standards. The calibrations were performed according to the EN ISO/IEC 17025 standard, which is a requirement for calibration laboratories. The observed relative measurement errors were 3.3% in the first time calibrations at the 95% confidence level. With the traceable basic calibration method, however, these errors can be reduced to under 0.5%, thus improving the integrity of on-line grammage measurements. Also a standardised algorithm, based on the experience from the performed calibrations, is proposed to ease the adjustment of the different grammage measurement systems. The calibration technique can basically be applied to all β-radiation based grammage measurements.