Ergebnisbericht der Task Force EHEC zur Aufkl?rung des EHEC O104:H4 Krankheitsausbruchs in Deutschland

This is a report on the results of the work of the German EHEC Task Force on the food safety side of the EHEC O104:H4 disease outbreak investigation in Germany. During the first phase of the outbreak investigation the main goal was to identify the contaminated food. To achieve this, two different strategies were followed in parallel. One approach was a detailed trace back analysis for all salad ingredients and raw vegetables that have been served to customers at five outbreak clusters in order to identify common food sources and delivery chains. The second approach was a trace forward analysis of the supply chains of a sprout producer in order to find out if he had delivered any outbreak clusters. Both approaches revealed that contaminated sprouts from a producer in Lower Saxony highly likely had caused the outbreak. Aim of the second investigation phase was to find and stop the source of the EHEC O104:H4 bacteria. The Task Force gave recommendations on source elimination measures and collected and analysed epidemiological information in order to find out when the source was active. Next to that, a detailed trace back for batches of suspicious seeds that had been used by the sprout producer was initiated. The results from this activity formed the basis for the tracing of seeds coordinated by the European Food Safety Authority, which revealed that fenugreek seeds imported from Egypt were the most likely common link between the EHEC O104:H4 outbreaks in Germany and France. In conclusion, the newly developed outbreak investigation strategy of the task Force EHEC with close collaboration between German federal and federal state authorities and between food safety authorities, health authorities and scientists was a recipe of success and can be a model for future food-borne outbreak investigations.     

Photoswitchable Azo- and Diazocine-Functionalized Derivatives of the VEGFR-2 Inhibitor Axitinib

In this study, we aimed at the application of the concept of photopharmacology to the approved vascular endothelial growth factor receptor (VEGFR)-2 kinase inhibitor axitinib. In a previous study, we found out that the photoisomerization of axitinib’s stilbene-like double bond is unidirectional in aqueous solution due to a competing irreversible [2+2]-cycloaddition. Therefore, we next set out to azologize axitinib by means of incorporating azobenzenes as well as diazocine moieties as photoresponsive elements. Conceptually, diazocines (bridged azobenzenes) show favorable photoswitching properties compared to standard azobenzenes because the thermodynamically stable Z-isomer usually is bioinactive, and back isomerization from the bioactive E-isomer occurs thermally. Here, we report on the development of different sulfur–diazocines and carbon–diazocines attached to the axitinib pharmacophore that allow switching the VEGFR-2 activity reversibly. For the best sulfur–diazocine, we could verify in a VEGFR-2 kinase assay that the Z-isomer is biologically inactive (IC₅₀ >> 10,000 nM), while significant VEGFR-2 inhibition can be observed after irradiation with blue light (405 nm), resulting in an IC₅₀ value of 214 nM. In summary, we could successfully develop reversibly photoswitchable kinase inhibitors that exhibit more than 40-fold differences in biological activities upon irradiation. Moreover, we demonstrate the potential advantage of diazocine photoswitches over standard azobenzenes.     

Relational housing across the North–South divide: learning between Albania,Uganda, and the UK

Abstract

In this paper, we examine how to understand housing as a relational process. Drawing on research in three diverse cities, we stage an unlikely dialogue that brings together narratives of housing across the global North–South divide. In doing so, we are concerned with thinking housing relationally in two broad senses: first, housing as a relational composite of economy, space, politics, legality and materials, structured by particular relations of power and resource inequality. Second, housing as a space of learning through comparison, which connects geographically and culturally in distinct cities. What do we learn about relational thinking with regards to housing when we compare it across the global North–South divide? In response, we explore a dialogue between a set of cities often off-the-map in debates on housing and urban research: Gateshead (UK), Kampala (Uganda) and Tirana (Albania). In comparing how housing is produced, distributed and inhabited, we seek to contribute to a wider understanding of the relationalities of housing.     

Compressive failure of UD-CFRP containing void defects: In situ SEM microanalysis

Here we present an in situ scanning electron microscopy (SEM) investigation of the compressive failure of unidirectional (UD) carbon fibre reinforced polymer (CFRP) composites with varying pre-existing void content. The experiments were carried out within a dual beam microscope, which couples a SEM with a focused ion beam (FIB), allowing sub-surface investigations of damage. In these tests, the specimen is monitored during the entire loading history, allowing the correlation of microstructural changes and the evolving load-displacement behaviour. Therefore, loading characteristics can be linked directly to failure events. Observations of the sequence of events leading to failure showed direct fibre deflection into a kinked shape eventually followed by fibre fracture. Failure of void-containing CFRP was shown to depend on the void shape as well as the proximity of the void to the kink band. In some cases voids stopped the propagation of kink bands, while in other cases the void caused the kink to deflect in a new direction. The failure structure was observed to change with time, both during hold-load segments as well as after unloading. Through cross-sectional ion beam milling in the unloaded state, the sub-surface damage was observed and shown to be similar to that observed at the surface.     

Association between alcohol-impaired driving enforcement-related strategies and alcohol-impaired driving

All states in the U.S. prohibit alcohol-impaired driving but active law enforcement is necessary for effectively reducing this behavior. Sobriety checkpoints, saturation patrols, open container laws, and media campaigns related to enforcement efforts are all enforcement-related strategies for reducing alcohol-impaired driving. We conducted surveys of all state patrol agencies and a representative sample of local law enforcement agencies to assess their use of alcohol-impaired driving enforcement-related strategies and to determine the relationship between these enforcement-related strategies and self-reported alcohol-impaired driving behavior obtained from the Behavioral Risk Factor Surveillance System. We found that sobriety checkpoints, saturation patrols, and enforcement of open container laws were associated with a lower prevalence of alcohol-impaired driving but, more importantly, a combination of enforcement-related strategies was associated with a greater decrease in alcohol-impaired driving than any individual enforcement-related activity. In addition, alcohol-impaired driving enforcement-related strategies were associated with decreased alcohol-impaired driving above and beyond their association with decreased binge drinking. Results suggest law enforcement agencies should give greater priority to using a combination of strategies rather than relying on any one individual enforcement activity.     

Time-dependent growth of biomimetic apatite on anodic TiO2 nanotubes

We report on the initial and later stages of apatite formation from simulated body fluid on titania with different surface morphologies (compact or nanotubular) and different crystal structures (anatase or amorphous). The nanotubular layers were fabricated by electrochemical anodization in fluoride-containing electrolytes. The study investigates the enhanced apatite deposition on titania nanotubes. In the initial stages of apatite growth, more nuclei are formed on the nanotubular surface than on flat compact TiO₂. While the crystallographic structure of the substrate plays a less important role than the morphology in the initial nucleation stages, it is of great importance in the later stages of apatite crystal growth. The nanotubular morphology combined with an anatase structure leads to the formation of apatite layers with a thickness of >6 nm in less than 2 days. No stable apatite layers can be observed on amorphous TiO₂ films, neither on compact nor on nanotubular substrates.XPS, FT-IR and XRD measurements reveal that carbonated hydroxyapatite (CHA) of low crystallinity is formed on annealed nanotubular and compact titania.Electrochemically grown and annealed TiO₂ nanotube arrays having anatase structure are expected to be a good precursor system for the formation of CHA and thus for the preparation of osseointegrative implants.     

Profiting from negawatts: Reducing absolute consumption and emissions through a performance-based energy economy

Current energy and GHG emissions policies either focus directly on emissions or promote renewable production and the implementation of specific efficiency measures. Meanwhile, the fundamental structure of the energy market based on profits through energy throughput remains largely unchallenged. This policy oversight prevents the transition to an energy economy in which profits are based on energy services delivered at the lowest energy cost: a performance-based energy economy (PBEE). The PBEE applies the combined concepts of the performance economy and energy services to the energy sector. Energy Service Companies (ESCOs) are discussed as an example of PBEE practices. The implications for energy suppliers and consumers as well as the conditions for PBEE diffusion and consequences for technological change are also explored. The expected environmental, social and economic benefits are described. However, absolute consumption and emissions reductions may prove elusive due to the rebound effect. In order to forestall rebound-led increases, complementary policy measures likely to lead to absolute reductions are required.