Stable repeated-batch production of recombinant dye-decolorizing peroxidase (rDyP) from Aspergillus oryzae

Recombinant Aspergillus oryzae expressing a dye-decolorizing peroxidase gene (dyp) was cultivated for repeated-batch production of recombinant dye-decolorizing peroxidase (rDyP) using maltose as a carbon source. High-level rDyP activity in limitation of carbon and nitrogen sources was maintained stably for 26 cycles of repeated 1-d batches of A. oryzae pellets without any additional pH control. Cultures maintained at 4 degrees C for 20 d resumed rDyP production following a single day of incubation. One liter filtrated crude rDyP containing 4600 U rDyP decolorized 5.07 g RBBR at the apparent decolorization rate of 17.7 mg l(-1) min(-1).     

Pseudomonas aeruginosa as a model microorganism for investigation of chemotactic behaviors in ecosystem

Motile bacteria sense changes in the concentration of chemicals in environments and respond in a behavioral manner. This behavioral response is called chemotaxis. Bacterial chemotaxis can be viewed as an important prelude to metabolism, prey-predator relationships, symbiosis, infections, and other ecological interactions in biological communities. Genome analysis reveals that a large number of environmental motile bacteria possess a number of genes involved in chemosensing and chemotatic signal transduction. Pseudomonas aeruginosa has a very complex chemosensory system with more than 20 chemotaxis (che) genes in five distinct clusters and 26 chemoreceptor (methyl-accepting chemotaxis protein [mcp]) genes. Among the 26 MCPs of P. aeruginosa, nine have been identified as MCPs for amino acids, inorganic phosphate, oxygen, ethylene, and volatile chlorinated aliphatic hydrocarbons, whereas 3 MCPs were demonstrated to be involved in biofilm formation and biosynthesis of type IV pilus. Six che genes are essential for chemotactic responses, while genes in Pil-Chp cluster and Wsp cluster are involved in type IV pilus synthesis and twitching motility and biofilm formation, respectively. P. aeruginosa, with its complex chemotaxis system, is a better model microorganism for investigating ecological aspects of chemotaxis in environmental bacteria than Escherichia coli and Salmonella enterica serovar Typhimurium, which possess a relatively simpler chemotaxis system.     

Pressureless all-solid-state sodium-ion battery consisting of sodium iron pyrophosphate glass-ceramic cathode and β″-alumina solid electrolyte composite

In recent years, the expansion of demand for lithium ion batteries has resulted in soaring prices of the constituent resources. From the viewpoint of safety, studies on all-solid-state batteries are actively being carried out. In this study, we succeeded in driving all-solid-state batteries derived from nontoxic oxide glasses at room temperature without requiring scarce resources such as lithium and cobalt. The main structure of the ceramic batteries with a simple structure in which Na₂FeP₂O₇ crystallized glass and β″-alumina solid solution are joined by pressureless cofiring at 550°C. During the crystallization of Na₂O-Fe₂O₃-P₂O₅ glass, fusion with the β″-alumina solid solution is achieved. Reversible charge and discharge of 80 mAh/g were achieved at room temperature. It is not necessary to apply pressure during cell preparation or the use of the batteries. Furthermore, the strong junction at the cathode and electrolyte interface does not peel off during charge and discharge over a long period of 623 cycles. Ex situ X-ray photoelectron spectroscopy revealed partial Fe⁴⁺ induction and a reversible charge and discharge reaction even after overcharging to 9 V. It was demonstrated that Na₂FeP₂O₇ is very stable against overcharging to 9 V.     

Impact of coffee preparation on total phenolic content in brewed coffee extracts and their contribution to the body’s antioxidant status

Food Science and Biotechnology - Although coffee has been reported as a major contributor to antioxidants in the diet, there are limited studies assessing how brewing methods and types of coffee...     

Low friction coefficient (approximately tan1°) of subaqueous debris flow in rotating flume tests and its mechanism

To reproduce a subaqueous debris flow with a friction coefficient of approximately tan1° in field, material with a huge volume may be involved. In this research, a steady subaqueous debris flow with a friction coefficient of approximately tan1° was produced by using the rotating flume experiment apparatus (Debris Flow Maker, DFM) with only 40 g of silicon powder. Subsequently, the mechanism of the low friction coefficient was interpreted with the hypothesis of dense agglomeration hitting (DAH). DAH means hitting of the dense agglomeration of dense flow on the flume's bottom plane. The following issues on DAH are considered: (I) the high velocity of the flume's bottom plane induces a strong hitting of particles; (II) the hitting elevates the liquid pressure near the boundary between the agglomeration and the flume's bottom plane; (III) the energy loss due to collision inside the particle agglomeration is limited. The experimental results provide an alternative method of producing subaqueous debris flow and an alternative measurement method of its friction coefficient; the hypothesis provides an alternative interpretation on the mechanism of low friction coefficient of subaqueous debris flow.     

Prolonged Aftereffect of Visuomotor Adaptation to Gradually Distorted Reality Displayed on a See-Through Head-Mounted Device

Growing evidence suggests that the gradual transformation of visuomotor association drives a distinct learning process from abrupt transformation in humans. In the current study, we developed a novel omnidirectional visuomotor transformation paradigm to study details of such difference in more realistic environment than conventional experimental systems. Participants were asked to perform a repetitive three-dimensional (3D) arm-reaching task to a target on a front touch panel, wearing a video see-through head-mounted device that displayed a rotating view of surrounding images. In the abrupt condition, the images were rotated by 20°; in the gradual condition, the rotation was increased in a stepwise-manner from 0° to 20°. In both conditions, pointing errors were decreased after adaptation. Further, although the aftereffect of adaptation was not different between conditions, the speed of decay of the aftereffect, which was quantified by an exponential fit, was slower in the gradual condition, suggesting longer-lasting aftereffects for the gradual shift.     

One Pot Cooking of Rice Grains for Preparation of Rice-Gel Samples Using a Small-Scale Viscosity Analyzer: Small-scale Rice-gel Preparation from Grains

Rice-gel prepared by the following three steps: rice grain cooking, shearing of the cooked rice, and cooling for gel formation, is expected as a novel food ingredient for modification of various food products such as bread and noodles. To meet the demand for high-throughput systems for research and developments on the new rice gels, herein we established a mini-cooking system for preparation of rice gel samples from grains using a small-scale viscosity analyzer (Rapid Visco Analyzer; RVA). Polished rice grains (4 g) were cooked with 22 mL of water in a canister, and the paddle equipped in the canister was rotated at 2,000 rpm for 30 min (80 °C was used as a representative) to shear the cooked rice. The sheared paste was cooled to 10 °C at 160 rpm, and the initial gelation property was evaluated by viscosity analysis within the RVA. Alternatively, the sheared paste was transferred to an acrylic mold and kept at 4 °C for 0, 1, 3, and 5 days for determination of the hardness with a compression test. Compressive forces required to penetrate 20 % thickness for three tested rice cultivars were measured, and the trend of the value shifts during preservation is similar to the corresponding trend obtained in 300-g grain scale laboratory tests, whereas the individual values were halved in the former. This small cooking method could offer a useful assay system for a rapid evaluation in the breeding programs and in the high-throughput screening of additives for the modification of properties.     

Physical meaning of ΔKRP and fatigue crack propagation in the residual stress distribution field

Previous papers have shown ΔK_RP to be a useful parameter describing fatigue crack propagation behavior, where ΔK_RP is an effective stress intensity factor range corresponding to the excess RPG load (re-tensile plastic zone's generated load) in which the retensile plastic zone appears under the loading process. In this paper, the relationship between ΔK_RP and the zone size ( ) (which is smaller between the tensile plastic zone at maximum load and the compressive plastic zone at minimum load) was investigated using a crack opening/closing simulation model so as to consider a physical meaning of ΔK_RP. As a result, it becomes clear that ΔK_RP dominates the zone size where fatigue damage mostly occurs. This result supports the following crack propagation equation

where C and m are material constants.Simulation and fatigue crack propagation tests were then carried out for compact tension (CT), center cracked tension (CCT) and four points bend (4PB) specimens under constant amplitude loading to obtain C and m values for HT-50 steel. Fatigue crack propagation tests were also carried out under constant amplitude loading using CCT specimens with residual stress distribution due to flame gas heating at the center line or edge lines. The T specimen introduced tensile residual stress at the tip of a notch, and the C specimen introduced compressive residual stress. It therefore becomes clear that tensile residual stress leads to a decrease in RPG load, while compressive residual stress leads to increase in RPG load, and that the simulation results are in good agreement with the experimental RPG load. It also becomes clear that simulated crack growth curve using the simulated and the above equation is in good agreement with the experimental curve. It is understood that tensile residual stress creates only a slight increase in crack propagation rate and compressive residual stress create a big decrease a crack propagation rate.