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21.
Yue WangAuthor Vitae Yasushi HasebeAuthor Vitae 《Sensors and actuators. B, Chemical》2011,155(2):722-729
Horseradish peroxidase (HRP) was chemically modified using cyanuric chloride (CC) as a linking agent onto a carbon felt (CF), which is a microelectrode ensemble of micro carbon fiber (>7 μm, diameter) with a random three-dimensional structure. The resulting HRP-modified CF (HRP-ccCF) exhibited well-defined redox waves based on the HRP heme FeIII/FeII redox couple at −0.23 V vs. Ag/AgCl (at pH 7.0), while the HRP-adsorbed CF (HRP-CF) showed no apparent redox couple in the same potential range, indicating that the chemical modification of HRP via CC facilitated the direct electron transfer (DET) between HRP and CF. The apparent heterogeneous electron transfer rate constant ks was estimated to be 35 s−1. Cyclic voltammetry and electrochemical impedance spectroscopy revealed that the interfacial properties (i.e., structure, morphology of enzyme-layer) of covalently modified HRP (HRP-ccCF) and physically adsorbed HRP (HRP-CF) are different, resulting in the difference in the electron transfer properties. The HRP-ccCF was successfully used as a working electrode unit in bioelectrocatalytic flow-through detector for highly sensitive amperometric determination of H2O2. Under the optimized conditions (i.e., applied potential, 0 V vs. Ag/AgCl; carrier flow rate, 3.25 ml/min; and carrier pH 7.0), the cathodic peak current of H2O2 linearly increased up to 3 μM (sensitivity, 1.94 μA/μM; the detection limit, 0.08 μM [S/N = 3]) with sample through-put of ca. 90 samples/h. 相似文献
22.
Simulation research on monomer agglomeration of nonmetallic inclusions in steel with a diffusion limited aggregation model 总被引:1,自引:0,他引:1
Hong Li Linxin Ning Juan Wen Jiongming Zhang Yasushi Sasaki Mitsutaka Hino 《北京科技大学学报(英文版)》2006,13(2):117-120
The monomer agglomeration of nonmetallic inclusions was simulated with a diffusion limited aggregation (DLA) model of the fractal theory. The simulation study with a random two-dimensional diffusion was carried out. The results indicate that the DLA model can be used for the simulation of agglomeration behavior of the cluster-type inclusions. The morphology of clusters was observed with SEM and compared with the simulated agglomerates. The modelling procedure of the DLA model is applicable for the agglomeration process. The uncertainty of agglomeration process and the persuasive average agglomerative ratio was analyzed. The factors about the agglomerative ratio with the collision path distance and the size of particles or seed were discussed. The adherence of the nonmetallic inclusions on the dam, the weir and the walls of a tundish, and the absorption of inclusions by stopper or nozzle were also discussed. 相似文献
23.
Yasushi Yagi Wataru Nishi Nels Benson Masahiko Yachida 《Machine Vision and Applications》2003,14(2):112-120
Described here is a method for estimating rolling and swaying motions of a mobile robot using optical flow. We have proposed
an image sensor with a hyperboloidal mirror for the vision-based navigation of a mobile robot. Its name is HyperOmni Vision.
The radial component of optical flow in HyperOmni Vision has a periodic characteristic. The circumferential component of optical
flow has a symmetric characteristic. The proposed method makes use of these characteristic to estimate robustly the rolling
and swaying motion of the mobile robot.
Correspondence to: Y. Yagi e-mail: y-yagi@sys.es.osaka-u.ac.jp 相似文献
24.
Yuika Suzuki Takuya Takeichi Kana Tanahashi Yoshinao Muro Yasushi Suga Tomoo Ogi Masashi Akiyama 《International journal of molecular sciences》2022,23(14)
Superficial epidermolytic ichthyosis (SEI) is an autosomal dominant inherited ichthyosis. SEI is caused by mutations in KRT2 and frequently shows erythroderma and widespread blistering at birth. We report the clinical manifestations of two patients from a Japanese family with SEI caused by a hotspot mutation, p.Glu487Lys, in KRT2. In addition, we summarize previous reports on SEI patients with the identical mutation. One of the two patients had disease onset at the age of 7 months. The other patient’s age of onset is unknown, but it was in childhood. Neither of the two patients showed erythroderma. To perform deep phenotyping, we studied the age of onset and the frequency of erythroderma in 34 reported SEI cases with the p.Glu487Lys mutation, including the present cases. Among the cases with sufficient clinical information, 44.4% of the cases that were due to p.Glu487Lys in KRT2 occurred at birth. Erythroderma was observed in 11.1% of the cases with p.Glu487Lys in KRT2. 相似文献
25.
26.
A platform of high accuracy and reliability is crucial for power amplifier (PA) designers to assess the characteristics of the PA prototype, and for the digital predistorter engineers to validate their models and algorithms. Digital processing techniques for building a test bed of high performance for PA characterization and predistortion (PD) are discussed in this article. We also proposed a novel technique to characterize the frequency responses of the vector signal generator and vector signal analyzer independently without using any other instrument. The techniques described in this article are quite useful, especially for narrowband vector transmitters and receivers, to improve their performance. These techniques were experimentally evaluated, both for characterization and PD of a class‐F PA. Test results show that using such techniques can successfully build a test bed of high accuracy and wide bandwidth. © 2012 Wiley Periodicals, Inc. Int J RF and Microwave CAE, 2013. 相似文献
27.
Li Xiang Makihara Yasushi Xu Chi Yagi Yasushi Ren Mingwu 《Multimedia Tools and Applications》2018,77(21):28333-28354
Multimedia Tools and Applications - Human age estimation from gait is expected to be an important technology for a variety of applications such as automatic customer counting for marketing research... 相似文献
28.
29.
Zhiling Li Yasushi Inoue Suyin Yang Naoko Yoshida Arata Katayama 《Journal of Bioscience and Bioengineering》2010,110(3):326-332
A mass balance and kinetic investigation of anaerobic dechlorination of pentachlorophenol (PCP) was undertaken using an enriched microbial consortium in a laboratory scale continuous flow column, as a model microbial permeable reactive barrier. The chlorine balance showed that 50 µM PCP was largely dechlorinated to phenol with the formation of a small quantity of 3-chlorophenol as an intermediate metabolite (hydraulic retention time 7.6 days), and the chlorine removal efficiency reached 36 µM d-1. When the initial PCP concentration was increased to 100 µM the chlorine removal efficiency increased 1.5 times. However, the dechlorination activity disappeared after 7.4 pore volumes (58 days), demonstrating the susceptibility of the dechlorination culture to high concentrations of PCP. Lactate released hydrogen as an electron donor during PCP dechlorination, with acetate, propionate, CO2 and CH4 as byproducts. The carbon balance showed that some of the organic carbon source (PCP, lactate) in the influent was converted to gas and utilized for biomass growth in addition to organic metabolites. The kinetic study was conducted in a batch culture and yielded 1.99 mg l-1 biomass growth per unit of chlorine consumption (µM). The Monod equation was well fitted to the specific growth rate of 1.38 d-1 and a half saturation constant of 0.29 µM. The organic chlorine removal rate in the batch culture was consistent with the results in the flow column, indicating the feasibility of and potential for in situ estimation and prediction through batch culture studies. 相似文献
30.
Wind force coefficients for designing porous canopy roofs have been investigated based on a series of wind tunnel experiments. Gable, troughed and mono-sloped roofs were tested. The roof models were made of 0.5 mm thick perforated duralumin plates, the porosity of which was changed from 0 to about 0.4. Overall aerodynamic forces and moments acting on the roof model were measured in a turbulent boundary layer with a six-component force balance for various wind directions. The results indicate that the wind loads on canopy roofs generally decrease with an increase in porosity of the roof. Assuming that the roof is rigid and supported by the four corner columns with no walls, the axial forces induced in the columns are regarded as the most important load effect for discussing the design wind loads. Two loading patterns causing the maximum tension and compression in the columns are considered. Based on a combination of the lift and moment coefficients, the design wind force coefficients on the windward and leeward halves of the roof are presented for the two loading patterns as a function of the roof pitch and porosity. The effect of porosity is taken into account as a reduction factor of the wind loads. 相似文献