A note on histamine levels in Turkish style fermented sausages

The histamine contents of Turkish style fermented sausages were determined on 46 samples of five different brands obtained from retail stores in Van in Turkey. Histamine was found in all samples in the range 19.64-87.47 mg/kg (mean 32.13 mg/kg). Histamine levels in the brands differed significantly (P<0.01). The results suggest that the histamine levels in the sausages were not hazardous in terms of public health although they may be a potential risk to sensitive individuals.     

Adsorption Studies of Chlorpropham and 3-Chloroaniline on Chemically Activated Halloysite

Chemically modified halloysite proved to be an effective adsorbent for the pesticide chlorpropham and 3-chloroaniline from an aqueous solution. Adsorption experiments were conducted using such procedures as the time-dependent （kinetic） procedure and the concentration dependent （isotherm） procedure. Results indicate that the adsorption process is related to the kind of the studied compound. The equilibrium data are well suited to a Freundlich isotherm in the case of both investigated compounds. Adsorption kinetics of chlorpropham and 3-chloroaniline on acid-treated halloysite was successfully described by pseudo-second order kinetic model and the model of Weber and Morris. From the present study, we suggest that the adsorption of chlorpropham and 3-chloroaniline on the modified halloysite is a rather complex process involving two steps： external mass transfer and intra-particle diffusion.     

Synthesis of mono-, di-, tri- and tetracarboxy azaphthalocyanines as potential dark quenchers

Mono-, di-, tri- and tetracarboxy-substituted metal-free azaphthalocyanines (AzaPc) were synthesized from 5,6-bis(diethylamino)pyrazine-2,3-dicarbonitrile and 6-(5,6-dicyano-3-(diethylamino)pyrazin-2-ylamino)hexanoic acid using a statistical condensation approach. AzaPc bearing eight diethylamino peripheral substituents was also isolated from the mixture. Analysis of the distribution of congeners in the statistical mixture using optimized HPLC method (Phenomenex Synergy RP Fusion column, acetonitrile/tetrahydrofuran/water (pH 5.5) 50:20:30) was performed. The analysis showed optimal ratios of starting materials to be 3:1 for AAAB, 1:3 for ABBB and 1:1 for AABB/ABAB types of the congeners. The distribution of the congeners corresponded well with calculated values indicating similar reactivity of both starting materials and no sterical constraint between adjacent isoindole units in the AzaPc ring. All investigated AzaPc showed no fluorescence, extremely low singlet oxygen quantum yields (Φ_? < 0.005) in monomeric form and strong absorption in a wide range from 300 nm to almost 700 nm. Such properties are highly promising for future investigation of these compounds as dark quenchers of fluorescence in DNA hybridization probes.     

Determination of optimum operating conditions and simulation of drying in a textile drying process

In this study, drying behavior of viscose yarn bobbins was investigated experimentally to specify the optimum drying conditions and a drying model was proposed for simulation of drying. The experiments were conducted in a pressurized hot-air bobbin dryer, which was designed and manufactured based on the dryers used in the textile industry. Drying process was performed for various drying parameters: bobbin diameter, drying temperature, drying pressure, and volumetric flow rate of drying air. The results show that total drying time is strongly dependent on drying pressure, drying temperature, and volumetric flow rate and increase at these parameters shortens the drying time considerably. The results also show that the minimum energy consumption is for lower values of drying temperature and drying pressure and modest and higher values of volumetric flow rate. Simulation results show that the most appropriate model in describing the drying curves of viscose yarn bobbins is the stretched exponential model.     

New voltage-mode universal filter and sinusoidal oscillator using only single DBTA

In this article, a new voltage-mode second-order universal frequency filter and sinusoidal oscillator using only single differential-input buffered and transconductance amplifier (DBTA) is presented. The proposed voltage-mode filter structure using single DBTA and four passive elements can provide all standard filter functions, i.e. low-, band-, high-pass, band-stop, and all-pass without changing the circuit topology and enables independent control of the quality factor Q using single passive element. The circuit requires the minimal number of active and passive elements with no conditions for component matching. By slight modification of the proposed filter structure, the new DBTA-based sinusoidal oscillator is easily obtained. The oscillation condition and the oscillation frequency are independently adjustable by different virtually grounded passive elements. The proposed sinusoidal oscillator employs only grounded capacitors. The passive and active sensitivities of all the proposed circuit configurations are low. PSPICE simulations using a BJT realisation of DBTA and experimental results based on commercially available amplifiers OPA860 and MAX436 are included, which prove the workability of the proposed circuits.     

An electronically tunable current-mode quadrature oscillator using PCAs

This article presents a new realisation of active RC sinusoidal oscillator with electronically tunable condition and frequency of oscillation (FO). As compared to the class of three resistors, two capacitors (3R-2C)-based canonic oscillators, the circuit proposed here uses only two resistors and two capacitors as the passive components and still provides non-interactive tuning laws for the condition of oscillation and the FO. The proposed circuit employs new bipolar programmable current amplifier as the active building block and is capable of simultaneously providing two explicit quadrature current outputs. SPICE simulation results have been included to verify the workability of the circuit as an oscillator and the tuning range of the FO.     

New resistorless and electronically tunable realization of dual-output VM all-pass filter using VDIBA

In this paper, a new active element called voltage differencing inverting buffered amplifier (VDIBA) is presented. Using single VDIBA and a capacitor, a new resistorless voltage-mode (VM) first-order all-pass filter (APF) is proposed, which provides both inverting and non-inverting outputs at the same configuration simultaneously. The pole frequency of the filter can be electronically controlled by means of bias current of the internal transconductance. No component-matching conditions are required and it has low sensitivity. In addition, the parasitic and loading effects are also investigated. By connecting two newly introduced APFs in open loop a novel second-order APF is proposed. As another application, the proposed VM APF is connected in cascade to a lossy integrator in a closed loop to design a four-phase quadrature oscillator. The theoretical results are verified by SPICE simulations using TSMC 0.18 μm level-7 CMOS process parameters with ±0.9 V supply voltages. Moreover, the behavior of the proposed VM APF was also experimentally measured using commercially available integrated circuit OPA860 by Texas Instruments.     

Understanding the Chena Hot Springs,Alaska, geothermal system using temperature and pressure data from exploration boreholes

Chena Hot Springs is a small, moderate temperature, deep circulating geothermal system, apparently typical of those associated to hot springs of interior Alaska. Multi-stage drilling was used in some exploration boreholes and was found to be useful for understanding subsurface flow characteristics and developing a conceptual model of the system. The results illustrate how temperature profiles illuminate varying pressure versus depth characteristics and can be used alone in cases where staged drilling is not practical. The extensive exploration activities helped define optimal fluid production and injection areas, and showed that the system could provide sufficient hot fluids (∼57 °C) to run a 400-kWe binary power plant, which came on line in 2006.     

Induced Zinc Loss Produces Heterogenous Biological Responses in Melanoma Cells

Zinc levels in serum and/or tissue are reported to be altered in melanoma with unknown effects on melanoma development and biology. The purpose of this study was to examine the effects of acute chelation of free intracellular zinc pools in melanoma cell lines Bowes and A375, as well as selected melanoma tissue explants with high or low intracellular free zinc. Zinc chelating agent TPEN at the concentration of 25 µM was employed during 48 h, which significantly reduced intracellular free zinc while decreasing melanoma cell proliferation, inducing G1/S arrest and cell damage leading to mitochondrial, caspase-dependent apoptosis. Chelation of free zinc was also associated with increased generation of superoxide in cell lines but not marked lysosomal membrane damage. Conversely, melanoma explant cultures mostly displayed time-dependent loss of lysosomal membrane integrity in the presence of slowly growing superoxide levels. Loss of free zinc-dependent p53 activity was similarly disparate in individual melanoma models. Surviving melanoma cells were arrested in the cell cycle, and varying proportions of them exhibited features characteristic of premature senescence, which increased in time despite zinc reloading. The present results show that melanoma cells with varying free zinc levels respond to its acute loss in a number of individual ways, reflecting activated mechanisms including oxidative stress, lysosomal damage, and p53 activity leading to heterogenous outcomes including cell death, transient, and/or permanent cell cycle arrest and premature senescence.     

Comparison of catalytic and noncatalytic pyrolysis and product yields of some waste biomass species

The products obtained by fast pyrolysis of biomass can be used as an energy source or chemical raw material. In this study, samples of hazelnut shells, tea bush, and hazelnut knot selected as waste biomass were from the cities of Trabzon and Rize in the Eastern Black Sea Region. Firstly, the waste biomass samples were granulated into four different particle sizes by milling and sieving operations. Fast pyrolysis of the samples with specific mixing rates was carried out in a fixed bed reactor. Additionally, 2 wt% vanadium (V) oxide (V₂O₅) was used as catalyst to maximize the yield of pyrolysis liquid products. The influence of temperature, heating rate, and particle size on fast pyrolysis yields under both catalytic and noncatalytic conditions were investigated and compared. While the amount of liquid product increased with the addition of catalyst, the amount of solid products decreased. It has been found that the temperature and heating rate parameters are very effective in liquid product yield. In all experiments, the maximum liquid yield was acquired at the same heating rate of 450°C min^?1 and the temperature of 450°C with particle size of 0.5 to 1.0 mm. The maximum pyrolysis liquid (bio‐oil) was obtained with catalytic pyrolysis, and this value was 60.58 wt%.