Author Keywords: City; History; Virtual; Urban; Town planning 相似文献
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61.
Sabrina Vantadori Andrea Carpinteri Karolina Gowacka Fabrizio Greco Tomasz Osiecki Camilla Ronchei Andrea Zanichelli 《Fatigue & Fracture of Engineering Materials & Structures》2021,44(1):3-13
By examining the state of the art, it can be realised that few research works have been done on the fracture behaviour of plastic composites reinforced with continuous glass fibres. Therefore, the present paper deals with the fracture toughness of a unidirectional glass fibre‐reinforced plastic (GFRP), such a parameter being analytically determined by means of the modified two‐parameter model (MTPM). The input data of the MTPM are obtained from an experimental campaign related to three‐point bending tests on single edge‐notched specimens characterised by different sizes. The novelty of this research work is that the MTPM, originally proposed for isotropic materials, is here employed to estimate the fracture toughness of GFRPs characterised by orthotropic mechanical properties. 相似文献
62.
Pawel G. Parzuchowski Magdalena Jurczyk‐Kowalska Joanna Ryszkowska Gabriel Rokicki 《应用聚合物科学杂志》2006,102(3):2904-2914
Carbonated soybean oil (CSO) containing five‐membered cyclic carbonate groups has been obtained in the reaction of epoxidized soybean oil with carbon dioxide in the presence of KI activated by 18‐crown‐6 under 6 MPa CO2 pressure at 130°C. The CSO was used for modification of bisphenol‐A based epoxy resin. The composition epoxide‐cyclic carbonate was cured using polyamine hardeners by one‐step and two‐step procedures. All cured compositions were characterized for their thermal and mechanical properties and compared with the parent epoxy network. The optimal properties were obtained for compositions containing CSO and cured by one‐step method when phase separation takes place. The mechanical properties were discussed in terms of morphology observed by SEM. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 102: 2904‐2914, 2006 相似文献
63.
Mariusz Mital Kosma Szutkowski Karolina Bossak-Ahmad Piotr Skrobecki Simon C. Drew Jarosaw Poznaski Igor Zhukov Tomasz Frczyk Wojciech Bal 《International journal of molecular sciences》2020,21(23)
The A peptide is a major beta-amyloid species in the human brain, forming toxic aggregates related to Alzheimer’s Disease. It also strongly chelates Cu(II) at the N-terminal Phe-Arg-His ATCUN motif, as demonstrated in A and A model peptides. The resulting complex resists ROS generation and exchange processes and may help protect synapses from copper-related oxidative damage. Structural characterization of Cu(II)A complexes by NMR would help elucidate their biological function, but is precluded by Cu(II) paramagneticism. Instead we used an isostructural diamagnetic Pd(II)-A complex as a model. To avoid a kinetic trapping of Pd(II) in an inappropriate transient structure, we designed an appropriate pH-dependent synthetic procedure for ATCUN Pd(II)A, controlled by CD, fluorescence and ESI-MS. Its assignments and structure at pH 6.5 were obtained by TOCSY, NOESY, ROESY, H-C HSQC and H-N HSQC NMR experiments, for natural abundance C and N isotopes, aided by corresponding experiments for Pd(II)-Phe-Arg-His. The square-planar Pd(II)-ATCUN coordination was confirmed, with the rest of the peptide mostly unstructured. The diffusion rates of A, Pd(II)-A and their mixture determined using PGSE-NMR experiment suggested that the Pd(II) complex forms a supramolecular assembly with the apopeptide. These results confirm that Pd(II) substitution enables NMR studies of structural aspects of Cu(II)-A complexes. 相似文献
64.
This Paper discusses the use of modern techniques to gather, convert and present the historic facts concerning the shape of the city—for an example of Warsaw. The aim of the project is to analyse chances of cooperative use of historic data base and contemporary software in intention to create virtual model of the old urban structure.
The deepest source of understanding the contemporary nature of the city is genuine consciousness of its history.
65.
Aleksandra Korbut Marcin Wodarczyk Karolina Rudnicka Aleksandra Szwed Przemysaw Pociski Monika Biernat Paulina Tymowicz-Grzyb Martyna Michalska Natalia Karska Sylwia Rodziewicz-Motowido Konrad Szustakiewicz 《International journal of molecular sciences》2021,22(24)
In this research, we describe the properties of three-component composite foam scaffolds based on poly(ε-caprolactone) (PCL) as a matrix and hydroxyapatite whiskers (HAP) and L-Lysine as fillers (PCL/HAP/Lys with wt% ratio 50/48/2). The scaffolds were prepared using a thermally induced phase separation technique supported by salt leaching (TIPS-SL). All materials were precisely characterized: porosity, density, water uptake, wettability, DSC, and TGA measurements and compression tests were carried out. The microstructure of the obtained scaffolds was analyzed via SEM. It was found that the PCL/HAP/Lys scaffold has a 45% higher Young’s modulus and better wettability compared to the PCL/HAP system. At the same time, the porosity of the system was ~90%. The osteoblast hFOB 1.19 cell response was also investigated in osteogenic conditions (39 °C) and the cytokine release profile of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α was determined. Modification of PCL scaffolds with HAP and L-Lysine significantly improved the proliferation of pre-osteoblasts cultured on such materials. 相似文献
66.
Rafa Maka Pawel Janas Karolina Sapo Teresa Janas Tadeusz Janas 《International journal of molecular sciences》2021,22(17)
RNA motifs may promote interactions with exosomes (EXO-motifs) and lipid rafts (RAFT-motifs) that are enriched in exosomal membranes. These interactions can promote selective RNA loading into exosomes. We quantified the affinity between RNA aptamers containing various EXO- and RAFT-motifs and membrane lipid rafts in a liposome model of exosomes by determining the dissociation constants. Analysis of the secondary structure of RNA molecules provided data about the possible location of EXO- and RAFT-motifs within the RNA structure. The affinity of RNAs containing RAFT-motifs (UUGU, UCCC, CUCC, CCCU) and some EXO-motifs (CCCU, UCCU) to rafted liposomes is higher in comparison to aptamers without these motifs, suggesting direct RNA-exosome interaction. We have confirmed these results through the determination of the dissociation constant values of exosome-RNA aptamer complexes. RNAs containing EXO-motifs GGAG or UGAG have substantially lower affinity to lipid rafts, suggesting indirect RNA-exosome interaction via RNA binding proteins. Bioinformatics analysis revealed RNA aptamers containing both raft- and miRNA-binding motifs and involvement of raft-binding motifs UCCCU and CUCCC. A strategy is proposed for using functional RNA aptamers (fRNAa) containing both RAFT-motif and a therapeutic motif (e.g., miRNA inhibitor) to selectively introduce RNAs into exosomes for fRNAa delivery to target cells for personalized therapy. 相似文献
67.
Karolina Siewiera Magdalena Labieniec-Watala Nina Wolska Hassan Kassassir Cezary Watala 《International journal of molecular sciences》2021,22(17)
Blood platelets are considered as promising candidates as easily-accessible biomarkers of mitochondrial functioning. However, their high sensitivity to various stimulus types may potentially affect mitochondrial respiration and lead to artefactual outcomes. Therefore, it is crucial to identify the factors associated with platelet preparation that may lead to changes in mitochondrial respiration. A combination of flow cytometry and advanced respirometry was used to examine the effect of blood anticoagulants, the media used to suspend isolated platelets, respiration buffers, storage time and ADP stimulation on platelet activation and platelet mitochondria respiration. Our results clearly show that all the mentioned factors can affect platelet mitochondrial respiration. Briefly, (i) the use of EDTA as anticoagulant led to a significant increase in the dissipative component of respiration (LEAK), (ii) the use of plasma for the suspension of isolated platelets with MiR05 as a respiration buffer allows high electron transfer capacity and low platelet activation, and (iii) ADP stimulation increases physiological coupling respiration (ROUTINE). Significant associations were observed between platelet activation markers and mitochondrial respiration at different preparation steps; however, the fact that these relationships were not always apparent suggests that the method of platelet preparation may have a greater impact on mitochondrial respiration than the platelet activation itself. 相似文献
68.
Karolina Labus 《Materials science & engineering. C, Materials for biological applications》2012,32(2):228-235
Laccase and tyrosinase were immobilized by adsorption and covalent attachment onto microfiltration membranes made of cellulosic and polyamide material. Amine, hydroxyl and carboxylic functional groups for covalent attachment were generated by plasma polymerization of allylamine, allyl alcohol and acrylic acid using mild plasma parameters. Mass analysis of the modified membranes, surface tension and FTIR-ATR spectra were used to show the presence of stable plasma polymer on the membrane surface. It was shown that untreated and plasma treated cellulosic membranes were unsuitable for laccase and tyrosinase immobilization. Both, immobilization of laccase onto polyamide membrane modified with AlNH2 and adsorption on the untreated membrane at pH 5.2 gave satisfactory and comparable results with better operational stability in 10 consecutive batch processes for covalently bound enzyme. In the case of tyrosinase, adsorption of the enzyme on the untreated PA at pH 7.0 was as effective as covalent binding onto PA-AlNH2 (in pH 7.0). Operational stability was tested in the presence of diphenolic substrate, which exhibits strong suicide inactivation towards the enzyme. It was shown that immobilized tyrosinase seems to be exceptionally stable in the presence of diphenolic substrate. 相似文献
69.
L. Mosińska M. Kowalska P. Popielarski K. Fabisiak K. Paprocki M. Szybowicz A. Wrzyszczyński G. K. Zhusupkalieva 《Materials Science-Poland》2013,31(1):146-150
The undoped, polycrystalline diamond films were deposited on tungsten wire substrates by hot filament chemical vapor deposition (HF CVD), using a precursor gas mixture of methanol with excess of hydrogen. The morphology and quality of the as-deposited films were monitored by scanning electron microscopy (SEM) and Raman spectroscopy. The surface morphology analyzed by SEM resembles a continuous and well faceted diamond film. Raman results showed essential differences in qualities of diamond films grown at different hydrocarbon concentrations. The electrochemical properties of diamond electrodes were examined with cyclic voltammetry (CV) and the electrochemical impedance spectroscopy (EIS). The CV experiments revealed a large chemical window (>~4.3 V) of undoped diamond. Analysis of the ferrocyanide-ferricyanide couple at a diamond electrode suggests some extent of electrochemical quasi-reversibility, but the rates of charge transfer across the diamond substrate interface vary with diamond quality. 相似文献
70.
Salmanowicz BP Adamski T Surma M Kaczmarek Z Karolina K Kuczyńska A Banaszak Z Lugowska B Majcher M Obuchowski W 《International journal of molecular sciences》2012,13(4):4186-4201
The influence of grain hardness, determined by using molecular markers and physical methods (near-infrared (NIR) technique and particle size index-PSI) on dough characteristics, which in turn were determined with the use of a farinograph and reomixer, as well as bread-making properties were studied. The material covered 24 winter wheat genotypes differing in grain hardness. The field experiment was conducted at standard and increased levels of nitrogen fertilization. Results of molecular analyses were in agreement with those obtained by the use of physical methods for soft-grained lines. Some lines classified as hard (by physical methods) appeared to have the wild-type Pina and Pinb alleles, similar to soft lines. Differences in dough and bread-making properties between lines classified as hard and soft on the basis of molecular data appeared to be of less significance than the differences between lines classified as hard and soft on the basis of physical analyses of grain texture. Values of relative grain hardness at the increased nitrogen fertilization level were significantly higher. At both fertilization levels the NIR parameter determining grain hardness was significantly positively correlated with the wet gluten and sedimentation values, with most of the rheological parameters and bread yield. Values of this parameter correlated with quality characteristics in a higher degree than values of particle size index. 相似文献