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排序方式: 共有879条查询结果,搜索用时 31 毫秒
41.
42.
Shoji Seki Mami Iwasaki Hiroto Makino Yasuhito Yahara Yoshitaka Miyazaki Katsuhiko Kamei Hayato Futakawa Makiko Nogami Nguyen Tran Canh Tung Tatsuro Hirokawa Mamiko Tsuji Yoshiharu Kawaguchi 《International journal of molecular sciences》2022,23(7)
Intervertebral disc (IVD) diseases are common spinal disorders that cause neck or back pain in the presence or absence of an underlying neurological disorder. IVD diseases develop on the basis of degeneration, and there are no established treatments for degeneration. IVD diseases may therefore represent a candidate for the application of regenerative medicine, potentially employing normal human dermal fibroblasts (NHDFs) induced to differentiate into nucleus pulposus (NP) cells. Here, we used a three-dimensional culture system to demonstrate that ectopic expression of MYC, KLF4, NOTO, SOX5, SOX6, and SOX9 in NHDFs generated NP-like cells, detected using Safranin-O staining. Quantitative PCR, microarray analysis, and fluorescence-activated cell sorting revealed that the induced NP cells exhibited a fully differentiated phenotype. These findings may significantly contribute to the development of effective strategies for treating IVD diseases. 相似文献
43.
Motoko Wakabayashi Hidehiko Wakabayashi Wolfgang Eisenreich Yasujiro Morimitsu Kikue Kubota Karl-Heinz Engel 《European Food Research and Technology》2011,232(5):753-760
To determine the capillary gas chromatographic (GC) elution order of the enantiomers of 4-mercapto-2-alkanones, racemic 4-acetylthio-2-alkanones
were synthesized and hydrolyzed by lipases to get enantio-enriched 4-mercapto-2-alkanones that were resolved by capillary
gas chromatography using a chiral stationary phase. The obtained enantio-enriched 4-mercapto-2-alkanones were esterified with
(S)-2-methoxy-2-(1-naphthyl)propionic acid ((S)-MαNP acid), and the HPLC analysis of MαNP thioesters revealed the same isomeric ratios as the chiral GC analysis of the
corresponding thiol enantiomers. The diastereoisomeric thioesters obtained were resolved by HPLC, and the absolute configurations
were determined on the basis of 1H NMR anisotropy effects. On the basis of these results, the GC elution order of the enantiomers of 4-mercapto-2-alkanones
and of the corresponding 4-acetylthio-2-alkanones could be determined. The combination of enzymatic resolution and MαNP thioesterification
proved to be a useful tool to determine the absolute configuration of secondary thiols. 相似文献
44.
Takuji Kobayashi Katsuhiko Sasaki 《Journal of Materials Science: Materials in Electronics》2009,20(4):343-353
This paper discusses uniaxial ratchetting deformation of lead-free solder alloy Sn–3Ag–0.5Cu and lead-containing solder alloy
Sn–37Pb, which were subjected to tension–compression loading with several stress amplitudes and stress ratios, minimum stress
over maximum stress. First the uniaxial ratchetting tests were conducted with three maximum stresses and four stress ratios.
All tests were conducted using cylindrical bulk specimens of the solder alloys at 313 K. The test results show that there
are differences in the ratchetting deformation behavior of the two solder alloys; the larger ratchetting strain occurs in
the lead-containing solder alloy than in the lead-free solder alloy. The ratchetting deformation was simulated by the dislocation
based constitutive model proposed by Estrin et al. (J Eng Mater Technol 118:441, 1996). The evolution equation of the back
stress employed in the constitutive model was modified considering a dynamic recovery term. The effect of the modification
of the back stress evolution is discussed by comparing the simulations with the corresponding experimental results. The simulations
suggest that the recovery term in the kinematic hardening rule plays an important role in fitting the simulation to the experimental
results of the ratchetting deformation of the solder alloys. 相似文献
45.
46.
Teruhisa Horita Haruo Kishimoto Katsuhiko Yamaji Manuel E. Brito Yueping Xiong Harumi Yokokawa 《Journal of power sources》2009,193(1):180-184
Reactivity of oxide scale on Fe-Cr alloy with Na-containing gases was examined to estimate the stability against sodium (Na): vapors of NaCl and Na2SO4 exposures with air flow at 1073 K. The identified reaction phases were Cr-Mn spinel, Cr2O3, and alloy from the X-ray diffraction of surface with no Na-reaction products. However, the protective oxide scales (Mn-Cr spinel and Cr2O3 layers) on the Fe-Cr alloy were partially decomposed by reacting with Na to form Na-compounds inside the oxide scale/alloy interfaces. In some parts, anomalous oxide scales were found around the oxide scale/Fe-Cr alloy interfaces, with forming Na-rich compounds: the compounds were distributed inner parts of oxide scales around oxide scale/alloy interfaces. The stability of oxide scales and degradation were discussed based on the observed distribution of elements. 相似文献
47.
48.
Katsuhiko Sano 《Journal of Logic, Language and Information》2009,18(4):515-539
The purpose of this paper is to argue that the hybrid formalism fits naturally in the context of David Lewis’s counterfactual
logic and that its introduction into this framework is desirable. This hybridization enables us to regard the inference “The
pig is Mary; Mary is pregnant; therefore the pig is pregnant” as a process of updating local information (which depends on
the given situation) by using global information (independent of the situation). Our hybridization also has the following
technical advantages: (i) it preserves the completeness and decidability of Lewis’s logic; (ii) it allows us to characterize
the Limit Assumption as a proof-rule with some side-conditions; and (iii) it enables us to establish a general Kripke completeness
result by using the proof-rule corresponding to the Limit Assumption. 相似文献
49.
50.
Avidin-containing polyelectrolyte microcapsules were prepared by a layer-by-layer deposition technique and uptake and release
of biotin-labelled fluorescein (b-FITC) was studied. The polyelectrolyte microcapsules were prepared by coating the surface
of calcium carbonate (CaCO3) microparticles containing avidin-poly(styrene sulfonate) (PSS) conjugate, followed by dissolution of CaCO3 core in an ethylenediaminetetraacetic acid solution. Release of avidin from the microcapsules was markedly suppressed due
to formation of a high molecular weight of avidin-PSS conjugate in the microcapsules. The uptake of b-FITC into the microcapsules
was highly enhanced through a strong binding of b-FITC to avidin, as compared to the uptake into avidin-free microcapsules.
Release of b-FITC from the microcapsules was accelerated upon addition of biotin, 2-iminobiotin, or lipoic acid in the solution
due to the competitive binding of the additives to the binding site of avidin. 相似文献