Human platelets contain a novel 47 kDa thiol-oxidase having affinity for genomic sterol-regulatory sequence

The present study provides evidence to support that human platelets possess a 47 kDa dual functional molecule having thiol-oxidase activity as well as high affinity for the SRE sequence in the human genome. On the basis of these as well as earlier results, we propose that Receptor 'Ck' dependent regulation of this dual functional 47 kDa molecule may provide a mechanism for the maintenance of cellular cholesterol homeostasis. Further, this mechanism may also explain the molecular basis of cholesterol-feedback lesion observed under premalignant conditions.     

Malignant transformation of NIH3T3 cells by overexpression of mot-2 protein

The murine mortalin genes, mot-1 and mot-2, are members of the hsp70 family of proteins and differ from each other by only two amino acid residues. Mot-1 is expressed in normal cells and has pancytosolic cellular distribution whereas mot-2 is found in the perinuclear region of immortal cells. We report here that a high level of expression of mot-2 protein resulted in malignant transformation of cells as analysed by anchorage independent growth and nude mice assays. A high level of protein expression is attributed to the 900 bp 3' untranslated region of the cDNA which does not have any transforming activity per se. Mortalin cDNA clones isolated from human transformed cells were also found to have transforming activity in similar assays and a high level of expression was apparent in some of the human immortalized cells that showed non-pancytosolic mortalin immunofluorescence. Taken together, the data suggest that nonpancytosolic mortalin may have a role in tumorigenesis.     

Fenton and Electro-Fenton Methods for Oxidation of H-Acid and Reactive Black 5

Oxidative treatment of H-acid (HA) and Reactive Black 5 (RB5) using Fenton reagent (Fe2+/H2O2) and the electro-Fenton (EF) method is reported. Optimization of doses of ferrous iron and hydrogen peroxide was carried out in each case using HA; and the oxidation of RB5 was also carried out under the optimized conditions. Approximately 71% chemical oxygen demand (COD) was removed in 2 h using the conventional Fenton method at optimized doses: Fe2+ = 0.3?g/L (5.37 mM), H2O2 = 6?mL/L (53.0 mM), H2O2/Fe2+ = 10. In contrast, more than 92% COD was removed in 15 min using the EF method with an optimized Fe2+ dose of 0.130?g/L (2.34 mM) and 8?ml/L (70.6 mM) of H2O2. The pseudo-first-order rate constants (k) for the Fenton reagent and EF method were 0.054 and 0.38?min?1. The COD removal through the EF method was seven times faster. The calculated energy requirement of the EF method was 0.82?kg?COD/kW?h at the minimum applied current (0.25 A) when approximately 92.5% COD was removed. In the case of RB5, about 67 and 87% COD was removed under optimized Fenton and electro-Fenton conditions, respectively. The higher efficiency of the EF method was attributed to incremental addition of Fe2+ and accompanying higher H2O2/Fe2+ molar ratio. The results are discussed in the light of the mechanism for Fenton’s oxidation.     

国际放射防护委员第2分委员会当前和今后工作的概述

本文对国际放射防护委员会第２分委员会当前和今后的工作作了概述，内容限于内照射剂量学领域，涉及：（１）公众成员的剂量；（２）工作人员的剂量；（３）对病人使用放射性同位素标记的药物所致的剂量。     

Design-Space Exploration for Block-Processing Based Temporal Partitioning of Run-Time Reconfigurable Systems

The reconfiguration capability of modern FPGA devices can be utilized to execute an application by partitioning it into multiple segments such that each segment is executed one after the other on the device. This division of an application into multiple reconfigurable segments is called temporal partitioning. We present an automated temporal partitioning technique for acyclic behavior level task graphs. To be effective, any behavior-level partitioning method should ensure that each temporal partition meets the underlying resource constraints. For this, a knowledge of the implementation cost of each task on the hardware should be known. Since multiple implementations of a task that differ in area and delay are possible, we perform design-space exploration to choose the best implementation of a task from among the available implementations.To overcome the high reconfiguration overhead of the current day FPGA devices, we propose integration of the temporal partitioning and design space exploration methodology with block-processing. Block-processing is used to process multiple blocks of data on each temporal partition so as to amortize the reconfiguration time. We focus on applications that can be represented as task graphs that have to be executed many times over a large set of input data. We have integrated block-processing in the temporal partitioning framework so that it also influences the design point selection for each task. However, this does not exclude usage of our system for designs for which block-processing is not possible. For both block-processing and non block-processing designs our algorithm selects the best possible design point to minimize the execution time of the design.We present an ILP-based methodology for the integrated temporal partitioning, design space exploration and block-processing technique that is solved to optimality for small sized design problems and in an iterative constraint satisfaction approach for large sized design problems. We demonstrate with extensive experimental results for the Discrete Cosine Transform (DCT) and random graphs the validity of our approach.     

The differences in human cumulative irritation responses to positive and negative irritant controls from three geographical locations

A retrospective analysis was conducted to evaluate whether studies from three geographically diverse locations have similar response profiles to the positive and negative controls in a standard 14‐day cumulative irritation study. The positive irritant control (0.1% sodium lauryl sulphate) and the negative control (0.9% sodium chloride, saline) data from seventeen 14‐day cumulative irritation studies were reviewed. The studies were compiled from three locations representing dry/hot, humid/hot, and dry/cold environments (Scottsdale, Arizona; St Petersburg, Florida; and Winnipeg, Manitoba, respectively). Irritation scores were generated by trained skin graders from a total of 442 subjects studied between 1999 and 2005. Cumulative irritation scores were reviewed and compared between study locations. The irritation scores for the positive and negative controls were not significantly different between locations. Temperature and relative humidity variation did not correlate significantly with overall irritation. However, the dryer climate (i.e. negative or low dew point) had a tendency to induce a higher overall irritation level for both positive and negative controls.     

FMR study of surface and isothermal annealing effects in amorphous Fe80B20−x C x and Fe82 B18−y Ge y alloys

Alterations in the bulk (surface) magnetic properties of amorphous Fe₈₀ B_20?x C _x (0 5x ? 10) and Fe₈₂ B_18?y Ge _y (0 ?y ? 6) alloys, caused by isothermal annealing (mechanical polishing) of the alloy ribbons at a temperature T _A = 0.5T _cr (whereT _cr is the crystallization temperature) for various lengths of time,t _a, ranging from 0 to 240 min, have been studied by measuring the corresponding changes in the ferromagnetic resonance (FMR) linewidth, AHPP, and resonance field,H _res. Polishing induces significant changes in bothH _res and ΔH _pp but leaves their composition dependence practically unaltered. By comparison, isothermal annealing has no influence onH _res for ali the investigated alloy compositions whereas it affects ΔH _pp to different extents depending on the alloy composition. Physical implications of these results are discussed in terms of the effect of surface and annealing treatments on various contributions toH _res and AHPP in the glassy alloys in question.     

Size and surface effects on the MRI relaxivity of manganese ferrite nanoparticle contrast agents

Superparamagnetic MnFe2O4 nanocrystals of different sizes were synthesized in high-boiling ether solvent and transferred into water using three different approaches. First, we applied a ligand exchange in order to form a water soluble polymer shell. Second, the particles were embedded into an amphiphilic polymer shell. Third, the nanoparticles were embedded into large micelles formed by lipids. Although all approaches lead to effective negative contrast enhancement, we observed significant differences concerning the magnitude of this effect. The transverse relaxivity, in particular r2*, is greatly higher for the micellar system compared to the polymer-coated particles using same-sized nanoparticles. We also observed an increase in transverse relaxivities with increasing particle size for the polymer-coated nanocrystals. The results are qualitatively compared with theoretical models describing the dependence of relaxivity on the size of magnetic spheres.