A case of venous aneurysm

A 70-year-old woman was referred to our hospital with a complaint of a soft mass at the right elbow. Venography demonstrated that the mass was connected with vein. This mass was surgically removed and histologically a marked reduction of both elastic fibers and the adventitia were observed in the aneurysmal wall. Venous aneurysm (VA) is different form varicose veins by many features. It was considered that congenital fragility of the venous walls was associated with the onset of VA. We classified VA into type-I VA (cystic ectasia) and type II (fusiform ectasia).     

Neuroprotection,Growth Factors and BDNF-TrkB Signalling in Retinal Degeneration

Neurotrophic factors play key roles in the development and survival of neurons. The potent neuroprotective effects of neurotrophic factors, including brain-derived neurotrophic factor (BDNF), ciliary neurotrophic factor (CNTF), glial cell-line derived neurotrophic factor (GDNF) and nerve growth factor (NGF), suggest that they are good therapeutic candidates for neurodegenerative diseases. Glaucoma is a neurodegenerative disease of the eye that causes irreversible blindness. It is characterized by damage to the optic nerve, usually due to high intraocular pressure (IOP), and progressive degeneration of retinal neurons called retinal ganglion cells (RGCs). Current therapy for glaucoma focuses on reduction of IOP, but neuroprotection may also be beneficial. BDNF is a powerful neuroprotective agent especially for RGCs. Exogenous application of BDNF to the retina and increased BDNF expression in retinal neurons using viral vector systems are both effective in protecting RGCs from damage. Furthermore, induction of BDNF expression by agents such as valproic acid has also been beneficial in promoting RGC survival. In this review, we discuss the therapeutic potential of neurotrophic factors in retinal diseases and focus on the differential roles of glial and neuronal TrkB in neuroprotection. We also discuss the role of neurotrophic factors in neuroregeneration.     

Analysis of proton exchange membrane fuel cell catalyst layers for reduction of platinum loading at Nissan

The biggest issue that must be addressed in promoting widespread use of fuel cell vehicles (FCVs) is to reduce the cost of the fuel cell system. Especially, it is of vital importance to reduce platinum (Pt) loading of catalyst layers (CLs) in the membrane electrode assembly (MEA) of a proton exchange membrane fuel cell (PEMFC). In order to lower the Pt loading of the MEA, mass transport of reactants related to the performance in high current density should be enhanced significantly as well as kinetics of the catalyst, which can result in the better Pt utilization and effectiveness. In this study, we summarized our analytical approach and methods for reduction of Pt loading in CLs. Microstructure, mass transport properties of the reactants, and their relation in CLs were elucidated by applying experimental analyses and computational methods. A simple CL model for I–V performance prediction was then established, where experimentally elucidated parameters of the microstructure and the properties in CLs were taken into account. Finally, we revealed the impact of lowering the Pt loading on the transport properties, polarization, and the I–V performance.     

The Chemistry of PCR Primers: Concept and Application

DNA is a typical organic compound with marked differences from other chemicals and biopolymers because DNA can be amplified by the enzyme polymerase. DNA can be, in principle, amplified from a single copy by the polymerase chain reaction (PCR). In this review, we focus our attention on the chemistry of PCR primers. Because PCR is basic technology in biology research fields, we sometimes use chemically labeled primers without any awareness of the chemistry they leave behind. We would like to emphasize that chemically labeled primers contain a lot of potential for different chemistry ideas and much study is still necessary to advance PCR for single-nucleotide polymorphism (SNP) typing, genetic diagnosis, and other fields. Two categories of primers, affinity-capture primers and signaling primers, are discussed from the viewpoints of their chemical concepts and applications. Affinity-capture primers are used for purification, isolation, and manipulation of PCR products by high specificity and affinity to the cognate molecules by molecule molecule interactions, whereas signaling primers report the hybridization and/or progress of PCR amplification by a signal change, in most cases by a fluorescence change. The content of this review may be useful for a better understanding of the chemistry of PCR primers and, more importantly, for the invention of novel PCR chemistry.     

Heterogeneity in Formation of Lignin. XIII. Formation of p-Hydroxyphenyl Lignin in Various Hardwoods Visualized by Microautoradiography

The heterogeneous nature of hardwood lignin was shown by visualizing the deposition process of p-hydroxyphenyl (H) lignin in the differentiating xylem of magnolia, beech, lilac and poplar. When p-glucocoumaryl alcohol-[arom. ring-2-³H], an efficient precursor of H units in lignin, was administered to the differentiating xylem of these trees, radioactivity was incorporated in the compound middle lamella region of vessel and fiber cell walls. The deposition of H units occurs only in the early stage of cell wall formation when the outer layer of the secondary wall is formed. H lignin deposits mainly within pectic substances and hemicellulose gel to form a highly condensed structural moiety.     

TWO-CRACK PROPAGATION PATHS IN A FUNCTIONALLY GRADED MATERIAL PLATE SUBJECTED TO THERMAL LOADINGS

Two-crack propagation paths in a ceramic/metal functionally graded material plate (FGP) under one-cycle temperature change of heating and cooling are considered. When the FGP is subjected to thermal shock, a single crack or multiple cracks often initiate on the ceramic surface during the cooling process and propagate in the FGP. Crack paths are influenced by the heating temperature conditions, a compositional profile of the FGP, the fracture toughness, interaction among multiple cracks, and so on. Transient thermal stresses are treated as a linear quasi-static thermoelastic problem for a plane-strain state. The crack paths are treated under fracture mechanics using the finite-element method. The effects of heating temperature conditions, a compositional profile of the FGP, the fracture toughness, and a crack space on the crack propagation pattern are discussed and are shown in figures.     

Human peripheral eosinophils express functional interferon-gamma receptors (IFN-gammaR)

In order to determine whether or not IFN-gammaR is associated with regulatory mechanisms on human eosinophil function, we examined the expression of functional IFN-gammaR on human peripheral eosinophils. In this study, peripheral blood eosinophils were obtained from seven normal controls and 12 patients (bronchial asthma, n = 9, and hypereosinophilic syndrome (HES), n = 3), and the purity of eosinophils was 97.11 +/- 2.31%, n = 19. We first showed that anti-IFN-gammaR alpha-chain MoAb reacted with all tested eosinophils of both normal controls and patients by flow cytometry analysis. We also showed expression of mRNA for the alpha-chain of IFN-gammaR in all purified eosinophils of six individuals. Further, to characterize IFN-gammaR on eosinophils, we did binding experiments with 125I-IFN-gamma on purified peripheral eosinophils. The linear Scatchard plot indicated a single type of high-affinity binding sites (dissociation constant (Kd) = 3.89-4.95 x 10(-10) M, numbers of binding sites = 183-233/cell, n = 3). To determine whether IFN-gammaR on eosinophils is functional, we examined surface eosinophilic cationic protein (ECP) and CD69 induction after IFN-gammaR ligation with recombinant human IFN-gamma (rhIFN-gamma) on eosinophils by flow cytometry. rhIFN-gamma stimulation significantly induced both ECP and CD69 expression on the 2-18 h-cultured eosinophils in a dose-dependent manner. Further, the effects of rhIFN-gamma stimulation were significantly blocked by both a neutralizing anti-IFN-gamma MoAb and a blocking anti-IFN-gammaR MoAb. These results suggest that human peripheral eosinophils express functional IFN-gammaR.     

Suitable parameters of single-mode optical fibre

A new method for determining suitable single-mode fibre parameters is examined. Suitable fibre parameters are determinable by minimising cabling- and splicing-loss increases. An eight-fibre cable was manufactured and the loss increases were measured. ? = 0.2% and V = 2.2 are the values selected using this method and for the experimental results.     

Estimation of compound classes in coal hydrogenation liquids by thin-layer chromatography

Compound classes in coal liquids were investigated by thin-layer chromatography coupled with a flame ionization detector (t.l.c.-f.i.d.). Individual t.l.c. peaks for coal liquids were identified as paraffins, aromatics, polar compounds and more strongly polar compounds or asphaltenes in order of increasing R_f values by comparison with specific compounds separated previously by conventional liquid chromatography. Concentrations of compound classes for a series of samples obtained by varying the hydrogenation time were estimated and good agreement was found with the results obtained by the USBM-API 60 liquid chromatography procedure. By using the t.l.c.-f.i.d. method, the relation of distribution of compound classes to the reaction conditions of coal hydrogenation could be derived quantitatively with relative ease.     

Er3+-doped cladding fibres and their taper componentapplications

A biconical taper amplifier utilizing an Er³⁺-doped cladding fiber is discussed. A maximum gain of 0.6 dB in a 2.4-Gb/s optical transmission experiment was obtained with a taper about 40 mm long. This result shows the potential of a new structure for Er³⁺ -doped coupling and amplifying components