Thraustochytrid as a potential source of carotenoids

Thraustochytrids, marine protists whose dominant genera are Thraustochytrium and Schizochytrium, belong to the kingdom Chromista and are known as an industrial source of DHA. We describe here that thraustochytrid strain KH105, isolated as a DHA producer, also accumulates significant levels of β-carotene and xanthophylls including canthaxanthin and astaxanthin. A4-d cultivation using a medium composed of 10% glucose and less than 0.3% of nitrogen sources in a half-concentration of seawater gave an astaxanthin production up to 6.1 mg/L, and canthaxanthin content reached more than 10 mg/L under conditions where a higher concentration of nitrogen sources (6%) was employed. It might be advantageous in mass production systems for these carotenoids to be extracted readily by simply suspending the cells with organic solvents such as acetone and chloroform. Analyses on the morphological and life history features of the KH105 strain revealed that it belongs to the genus Schizochytrium. This particular species of thraustochytrids is thus considered to be a promising source of xanthophylls as well as DHA for use in the food industry.     

Data Center as a Key Player of a District Electric Power and Heat Network System： A Comparison in Urban and Suburb Regions

A DC （data center） demands air-conditioning power as large as the 1/3-1/2 of total electricity consumption. Thus, energy saving of cooling power of DC yields considerable effect on both economic and environmental views. PV （Photovoltaic） and absorption refrigerator with CGS （cogeneration systems） or gas boiler are possible power saving options. The waste warm air from DC would be utilized for greenhouse heating when DC and greenhouse locate near in the suburbs. In this study, the authors develop an energy network model to assess the potential contribution of DC as a major electric power and chilled air consumer as well as the warm air supplier in a district to the energy efficiency improvement. The evaporation heat of LNG （liquefied natural gas） utilization is also considered as well as PV, CGS. This model is applied to the cases of the urban area in Tokyo which involves athletic center, shops and hospital and the suburbs including greenhouse and then compared.     

The Effectiveness of Latent Semantic Analysis for Building Up a Bottom-up Taxonomy from Folksonomy Tags

In this paper, we evaluate the effectiveness of a semantic smoothing technique to organize folksonomy tags. Folksonomy tags have no explicit relations and vary because they form uncontrolled vocabulary. We discriminates so-called subjective tags like “cool” and “fun” from folksonomy tags without any extra knowledge other than folksonomy triples and use the level of tag generalization to form the objective tags into a hierarchy. We verify that entropy of folksonomy tags is an effective measure for discriminating subjective folksonomy tags. Our hierarchical tag allocation method guarantees the number of children nodes and increases the number of available paths to a target node compared to an existing tree allocation method for folksonomy tags.     

Closed-form forward kinematics solutions of a 4-DOF parallel robot

It is well known that the forward kinematics of parallel robots is a very difficult problem. Closed-form forward kinematics solutions have been reported only to a few special classes of parallel robots. This paper presents closed-form forward kinematics solutions of a 4-DOF parallel robot H4. A 16th order polynomial in a single variable is derived to solve the forward kinematics of the H4. The 16 roots of the polynomial lead to at most 16 different forward kinematics solutions. A numerical verification is also presented.     

Towards the appropriate human-centred information systems: A case study of the Japanese retail industry

The industries of Japan have developed by learning from Western industries, especially the USA, and by implementing many of their concepts and technologies. However, Japanese industries have often implemented these concepts and technologies in a very different way from the USA. For example, while the USA uses information systems in retail industries as a tool by which data are collected and analysed to control the market, in Japan this same technology is considered rather as a learning device to interpret the market. While in the USA the market is seen as a natural phenomenon capable of being controlled, the Japanese see it as an ambiguous phenomenon that is ever changing and is not capable of being controlled. Rather it is important to feel the change in the market itself.This paper introduces human centredness to the information system, and argues against modern rationalism, i.e. human versus technology, taking the case of use of POS data from the POS system (point of sale: a system that collects data on both the customer and goods sold by scanning bar codes that are attached to the surface of the goods) by the eminent Japanese retailer, Ito-Yokado. It emphasises an interactive concept of interaction between human and technology of the postmodern paradigm.     

Slow eye movement as a possible predictor of reaction delays to auditory warning alarms in a drowsy state

In recently developed intelligent vehicles, warning alarms are often used to prompt avoidance behaviours from drivers facing imminent hazardous situations. However, when critical reaction delays to auditory stimulation are anticipated, the alarm should be activated earlier to compensate for such delays. It was found that reaction times to an auditory stimulus significantly increased in the presence of slow eye movement (SEM), which is known to occur frequently during the wake-sleep transition. The reaction delay could not be attributed to temporal effects such as fatigue and was invariant regardless of response effectors (finger or foot). Moreover, it was found that applied pedal force decreased immediately after an auditory stimulus interrupted SEM. Consequently, it was concluded that SEM can be a good predictor of reaction delays to auditory warning alarms when drivers are in a drowsy state. STATEMENT OF RELEVANCE: The present study demonstrated that simple auditory reaction time significantly increased when SEM emerged. In the design of vehicle safety systems using warning alarms to prompt avoidance behaviours from drivers, such reaction delays during SEM must be taken into account.     

Localization of glycosaminoglycans and CD44 in the human lacrimal gland

Recent analyses of tears indicate the presence of glycosaminoglycans as their components, but their origin remains unknown. To further understand the origin of these tear components, we investigated by immunohistochemical techniques the localization of glycosaminoglycans and CD44 human lacrimal glands obtained from 20 cadavers at autopsy. Monoclonal antibodies to CD44, a receptor for hyaluronic acid, dermatan sulfate, chondroitin sulfate, and keratan sulfate were applied to the tissue. Hyaluronic acid binding region was also used for the staining of hyaluronic acid. By light microscopy, immunoreactivity for CD44 was mostly detected on the baso-lateral membrane of acinar and ductal cells, and the vascular endothelium in the interstitium. Positive staining of hyaluronic acid was associated intensely with the basal membrane of acinar and ductal cells and weakly, faintly or not at all with their lateral membrane. Positive staining of hyaluronic acid and immunoreactivity for dermatan sulfate were detected in interstitial fibrous structures; particularly, the former was intense in the perivascular fibrous structures, and the latter along the periparenchimal fibrous structures. Immunoreactivity for chondroitin sulfate and keratan sulfate was seen in some acinar cells and the acinar and ductal lumen. By electron microscopy, immunogold particles indication chondroitin sulfate sulfate or keratan sulfate labeled secretory granules of the acinar cells. Considering the fact that CD44 is a receptor molecule for hyaluronic acid, the association of hyaluronic acid with the basal membrane and weakly or faintly with the lateral membrane of acinar and ductal cells may be attributed to the expression of CD44 on the baso-lateral membrane of the cells. Moreover, the presence of immunoreactivity for chondroitin sulfate and keratan sulfate in secretory granules of acinar cells and their lumens suggests that tears from the lacrimal gland contain these glycosaminoglycans.     

Intermittent Hypoxia Increased the Expression of DBH and PNMT in Neuroblastoma Cells via MicroRNA-375-Mediated Mechanism

Sleep apnea syndrome (SAS), characterized by recurrent episodes of oxygen desaturation and reoxygenation (intermittent hypoxia (IH)), is a risk factor for hypertension and insulin resistance. We report a correlation between IH and insulin resistance/diabetes. However, the reason why hypertension is induced by IH is elusive. Here, we investigated the effect of IH on the expression of catecholamine-metabolizing enzymes using an in vitro IH system. Human and mouse neuroblastoma cells (NB-1 and Neuro-2a) were exposed to IH or normoxia for 24 h. Real-time RT-PCR revealed that IH significantly increased the mRNA levels of dopamine β-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) in both NB-1 and Neuro-2a. Western blot showed that the expression of DBH and PNMT in the NB-1 cells was significantly increased by IH. Reporter assays revealed that promoter activities of DBH and PNMT were not increased by IH. The miR-375 level of IH-treated cells was significantly decreased relative to that of normoxia-treated cells. The IH-induced up-regulation of DBH and PNMT was abolished by the introduction of the miR-375 mimic, but not by the control RNA. These results indicate that IH stress increases levels of DBH and PNMT via the inhibition of miR-375-mediated mRNA degradation, potentially playing a role in the emergence of hypertension in SAS patients.