Power Leveling Using Ozone Storage Method

It is possible to detach ozone from silica gel under the same temperature and same pressure conditions in which the silica gel adsorbed ozone (Murai et al., 2000 Murai, A. and Tahara, N. Storage Method for Ozone oriented to Power Loads Leveling. 2000 International Ozone Association Pan American Group Conference. Proceedings of, Orlando, Florida, USA Session 9. [Google Scholar], 2001 Murai, A., Tahara, N. and Nakajima, T. 2001. Electric Power Loads Leveling by Ozone Storage. J.Inst.Elec.Install.Engi.Japan, 21: 516–522.  [Google Scholar], 2002 Murai, A., Tahara, N. and Nakajima, T. 2002. Ozone Storage Oriented to Power Load Leveling. Ozone Sci. Eng., 23: 171–180.  [Google Scholar]). The addition of extra energy is unnecessary. We changed the temperature of the silica gel and measured the differences in the amount of stored ozone. The amount of adsorbed ozone became linear, as determined by the van't Hoff relationship. A computer program was developed to simulate the characteristics of the storage and removal of ozone. The results of the calculation demonstrated these characteristics. This system confirms an inexpensive construction method of storing ozone in a sewage plant. This method is effective for power load leveling.     

LORES-2: A Logic Reorganization System

LORES-2 is a logic reorganization system which greatly contributes to the effective automation of logic design. LORES-2 uses a macro-expansion technique to help designers transform printed-circuit assembly logic composed of SSI and MSI circuits into master-slice LSI logic circuits. The number of gates of the most reorganized LSI circuit falls within ± 20 percent of the number of gates of the original circuit. When ROMS and/or PLAs are not-allowed on the target LSI circuit, those elements are converted into optimized, multilevel random logic using logic minimization, factoring and macro-expansion techniques.     

Flavour volatiles of Assam CTC black teas manufactured from different plucking standards and orthodox teas manufactured from different altitudes of Darjeeling

Biochemical correlations between volatile flavour constituents (VFC) of CTC bluck teas manufactured from fine and coarse plucking of Assam tea and orthodox teas fiom three different altitudes were carried out by GC-MS. In general, fine plucking produced more monoterpenoids and less nonterpenoids than coarse plucking. The volatile flavour compounds were similar in all types of tea except that geranoic acid was detected in Darjeeling teas. The different ecosystems of Assam and Darjeeling have been found to have a pronounced impact upon aroma concentration. However, major diferences between the volatiles of teas from the different altitudes at Darjeeliny appear to be not so significant.     

N-terminal vacuolar sorting signal at the mouse antibody alters the N-linked glycosylation pattern in suspension-cultured tobacco BY2 cells

Recombinant DNA technology enables the use of plants as the host for the production of pharmaceutical proteins, such as antibodies. The glycosylation of recombinant proteins plays physiological and biological roles. However, because glycosylation in plants is different from that in human cells, the development of glycoengineering is required. In plant cells, glycan structures are shown to correlate with the localization of the recombinant protein produced. In this study, the vacuolar sorting signal (VSS) of sporamin was fused to the heavy (H) and light (L) chains of a mouse monoclonal antibody (mAb), and the mAb was produced in suspension-cultured tobacco BY2 cells. The sugar chain structures were determined by high-performance liquid chromatography, exoglycosidase digestion, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Typical plant glycans with α1,3-fucosylation and/or β1,2-xylosylation derived from mAb with the VSS-fused H-chain (mIgG1000) and mAb with the VSS-fused H- and L-chain (mIgG1010) occupied the large amount of the total N-glycans, 72.1% and 85.0%, respectively, such as those derived from mAb without VSS (mIgG0000), 74.6% (Fujiyama et al., J. Biosci. Bioeng., 101, 212-218, 2006). In contrast, the typical plant glycan structure Man?FucXylGlcNAc? particularly in vacuoles accounted for 37.8% of the total sugar chains derived from mIgG1000 and 58.5% of those derived from mIgG1010 compared with 24.3% of those derived from mIgG0000. These results suggest that the sporamin signal peptide fused to mAb acts as a VSS and leads to the increase in the amount of Man?FucXylGlcNAc?, which is the main N-glycan structure in vacuoles.     

In Planta production of immunogenic poliovirus peptide using tobacco mosaic virus-based vector system

The tobacco mosaic virus (TMV) provides an attractive means of producing foreign peptides in plants. In this study, a TMV-based vector was designed such that a fragment encoding 15 amino acids of the poliovirus peptide (PVP) derived from the viral capsid proteins VP3 and VP1 of poliovirus type 1 Sabin was inserted downstream of the six-base 3' context nucleotide sequence of the TMV coat protein (CP) gene. This design allowed readthrough at the amber stop codon, thereby producing the chimeric TMV particle with both intact CP and CP-fusion protein (CP-PVP) in Nicotiana tabacum cv. Samsun infected with the TMV vector. The TMVCP-PVP virus particle induced antibodies against PVP as well as TMVCP in mice after intraperitoneal immunization. These data illustrate the potential of the readthrough translation system with TMVCP for antigen presentation and vaccine production.     

Preparation of active layers in polymer solar cells by aerosol jet printing

Active layers of polymer solar cells were prepared by aerosol jet printing of organic inks. Various solvents and additives with high boiling points were screened for the preparation of high-quality polymer films. The effects on device performance of treating the films by thermal and solvent vapor annealing were also investigated. The components of the solvent were important for controlling the drying rate of the liquid films, reducing the number of particle-like protrusions on the film surface, and realizing high molecular ordering in the polymer phases. The optimized solar cell device with poly(3-hexylthiophene) and a C(60) derivative showed a high fill factor of 67% and power conversion efficiency of 2.53% without thermal annealing. The combination of poly[N-9-heptadecanyl-2,7-carbazole-alt-3,6-bis(thiophen-5-yl)-2,5-diethylhexyl-2,5-dihydropyrrolo-[3,4-]pyrrole-1,4-dione] and a C(70) derivative led to power conversion efficiency of 3.92 and 3.14% for device areas of 0.03 and 1 cm(2), respectively.