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排序方式: 共有898条查询结果,搜索用时 78 毫秒
81.
Y Kitagawa Y Shirai H Ito T Narita T Aoki K Takahira 《Canadian Metallurgical Quarterly》1997,64(5):483-486
Cell cycle analysis using flow cytometer (FCMX) was performed to evaluate the influence of cyclosporin A (CsA) on cellular proliferation during compensatory renal growth (CRG) in uninephrectomized (Ux) rats. CsA (50 mg/kg/day) was administered subcutaneously for 7 days and animals were scarificed 0, 12, 24, 48, 72, 120 and 168 h after Ux. The percent changes in the S (DNA synthesis) and G2M (DNA replication) phases in the contralateral kidney were analyzed. The cortical cells entering the S and G2M phases showed significant differences between CsA and vehicle groups (p = 0.0001, p = 0.001, respectively), but the medullary cells entering the S and G2M phases showed no significant differences between the 2 groups (p = 1.000, respectively). In the cortex, the CsA group showed a significant decrease in the S (at 12, 72 and 120 h) and G2M phases (at 24, 72 and 120 h) compared to the vehicle group (p < 0.05, respectively), but there was no significant difference in the medulla. In conclusion, cellular proliferation is suppressed in cortical cells by CsA. 相似文献
82.
Okayasu T. Watanabe D. Ono A. Shibata M. Hayase Y. Shirai T. Inoue T. Ueno K. Hosoi F. Akiyama T. 《Lightwave Technology, Journal of》2004,22(9):2064-2082
A protocol-free parallel optical interconnecting module is introduced as a solution to solve memory test system transmission bottlenecks. The optical transmission system flexibly suited for a memory test system is reviewed and discussed. A parallel optical module capable of transmitting from dc to 34.1Gb/s (4.267 Gb/s /spl times/8 ch) has been developed. A data transmission throughput density per unit volume of 19 Gb/s/cm/sup 3/ is achieved. A random jitter of less than 3-ps root-mean-square is also achieved. Furthermore, high-density optical connector, high-density optical fiber cable, fiber guides, and cable management/reinforcement members suited for mechanical requirements of the memory test system have been developed. 相似文献
83.
Edson L. da Silva Terezinha J.C. Neiva Mutsuko Shirai Junji Terao Dulcinia S.P. Abdalla 《Food research international (Ottawa, Ont.)》2008,41(10):973-979
Yerba mate extract (Ilex paraguariensis) is a source of phenolic compounds that possesses in vitro antioxidant activities and may contribute to a reduction in the risk of cardiovascular disease. In this study we examined the acute effects of the consumption of mate infusion on ex vivo plasma and low-density lipoprotein (LDL) oxidation, plasma antioxidant capacity, and platelet aggregation. Twelve healthy fasted subjects ingested 500 mL of mate infusion and blood samples were collected before and 1 h after mate intake. Lipid peroxidation of plasma and LDL was monitored by the measurement of cholesteryl-ester hydroperoxides (CE-OOH) and cholesterol oxides. The plasma antioxidant capacity was measured as ferric-reducing antioxidant potential (FRAP). Platelet aggregation was evaluated in platelet-rich plasma stimulated with adenosine diphosphate and coagulation was tested in platelet-poor plasma. Ingestion of mate infusion diminished the ex vivo oxidizability of both plasma and LDL particles. After mate intake, the CE-OOH levels were around 50% lower in plasma oxidized with copper or 2,2′-azobis[2-amidine-propane-hydrochloride] (AAPH) and the lag time to plasma oxidation increased 2-fold (P < 0.05). Copper- and AAPH-induced LDL peroxidation were also inhibited by around 50% and 20%, respectively, after mate consumption (P < 0.05). The levels of various oxysterols were significantly reduced in oxidized-plasma and LDL (P < 0.05) and FRAP increased by 7.7% after mate intake (P < 0.01). However, mate consumption did not inhibit platelet aggregation or blood coagulation. In summary, intake of yerba mate infusion improved the antioxidant capacity and the resistance of plasma and LDL particles to ex vivo lipid peroxidation. 相似文献
84.
Seiji Yamashita Hideo Watanabe Takashi Shirai Masayoshi Fuji Minoru Takahashi 《Advanced Powder Technology》2011,22(2):271-276
We have successfully synthesized zinc oxide microrods perpendicularly oriented on hexagonal ZnO sheets by a simple heat treatment approach using LDH (layered double hydroxide) precursor in an aqueous solution. The synthesized ZnO microrods have an average diameter of 500 nm and length of 2–3 μm, and form highly-oriented array. In this work, the effect of heating temperature and time on morphology and orientation of ZnO microrods was studied experimentally and the formation mechanism was discussed in detail. Transformation from hexagonal precursor to ZnO microrods can be attributed to dissolution and re-precipitation of the precursor, which should be caused by its thermal unstability under heating temperature. 相似文献
85.
Kumaki Y Ogawa M Hirano T Yoshikawa K Iwasawa N Yagi T Hakamata W Oku T Nishio T 《Journal of Bioscience and Bioengineering》2011,111(2):134-139
The chromosomal DNA of the syntrophic thermophile Symbiobacterium thermophilum contains open reading frames of the genes encoding family M42 aminopeptidases, Pep1079, Pep1080, and Pep1081. To characterize these peptidases, the genes were cloned into Escherichia coli and overexpressed. Our experiments using the recombinant proteins confirmed that Pep1079, Pep1080, and Pep1081 are components of arginyl or lysinyl aminopeptidases that require Co2+ for enzymatic activity. Coexistence of Pep1079 and Pep1080 is necessary for expressing high peptidase activity. Pep1081 enhances the activity of Pep1079 and Pep1080. 相似文献
86.
Fumitaka Goto Katsunori Shirai Masaya Ichimura 《Solar Energy Materials & Solar Cells》1998,50(1-4):147-153
Using the electrochemical deposition method, CdS thin films were deposited from acid solutions (pH = 2.5) containing CdS04 and Na2S203 on indium-oxide coated glass substrates. These films were annealed in N2, air, or O2 atmosphere at 200–500°C for 30 min. Photoluminescence spectra were measured at 77 K. For the films annealed in N2, the band edge emission became weaker and the luminescence due to defects shifted to longer wavelengths as the annealing temperature was raised above 300°C. However, for the films annealed in air or O2, the band edge emission was observed strongly irrespective of the annealing temperature and the luminescence due to defects was weak. Thus the O2 annealing is useful for the defects reduction. 相似文献
87.
A plasmonic biosensor chip of silver-coated PMMA grating with a zinc oxide (ZnO) overlayer is fabricated for surface plasmon field-enhanced fluorescence (SPF) detection of Cy5-labeled green fluorescent protein (GFP). A bispecific antibody (anti-GFP x anti-ZnO antibody) prepared in our lab is densely immobilized on the sensor chip for GFP detection. The sensitivity of the plasmonic biosensors is improved due to densely packed antibodies and ZnO-coating that suppresses nonspecific protein adsorption and fluorescent quenching. With the ZnO-coated plasmonic chip, Cy5-labeled GFP of 10 pM can be detected through SPF. This sensitivity is 100 higher compared with the normal fluorescent detection on a ZnO-coated glass slide. 相似文献
88.
Yoshikawa M Seki H Inoue K Matsuda K Tanahashi Y Sako H Nanen Y Kato M Kimoto T 《Applied spectroscopy》2011,65(5):543-548
We used Fourier transform infrared (FT-IR) spectroscopy to characterize silicon dioxide (SiO(2)) films on a 4H-SiC(0001) Si face. We found that the peak frequency of the transverse optical (TO) phonon in SiO(2) films grown on a 4H-SiC substrate agrees well with that in SiO(2) films grown on a Si substrate, whereas the peak frequency of the longitudinal optical (LO) phonon in SiO(2) films on a 4H-SiC substrate is red-shifted by approximately 50 cm(-1) relative to that in SiO(2) films on a Si substrate. We concluded that this red-shift of the LO phonon is mainly caused by a change in inhomogeneity due to a decrease in density in the SiO(2) films. Furthermore, cathodoluminescence (CL) spectroscopy results indicated that the channel mobility of the SiC metal-oxide-semiconductor field-effect transistor (MOSFET) decreases roughly in proportion to the increase in the intensity of the CL peak at 460 and 490 nm, which is attributed to the increase in the number of oxygen vacancy centers (OVCs). FT-IR and CL spectroscopies provide us with a large amount of data on OVCs in the SiO(2) films on a 4H-SiC substrate. 相似文献
89.
Significance of the detection of staphylococcal enterotoxin A gene in low fat milk which caused a serious outbreak of food poisoning 总被引:1,自引:0,他引:1
Yamashita K Kanazawa Y Ueno M Ohta H Kitaguchi M Kawakami T Iwasaki K Tsujisawa E Morino Y Tabita K 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2003,44(4):186-190
In June 2000, there was a large-scale outbreak of food poisoning after consumption of Snow Brand low fat milk. In the evening of a day the incident made public, some cartons of low fat milk were brought to our laboratory for examination. Next day, we detected only staphylococcal enterotoxin (SE) A gene among SE (A-E) genes by PCR in left-over milk samples or samples from the same lots that patients had consumed. We presumed that the outbreak was caused by the intake of SEA. We subsequently confirmed the presence of SEA in these samples. To investigate the existence of SE (A-E) genes in milk, we examined 100 samples of commercial low fat milk and milk by PCR, but none of the genes was detected. We estimated the detection limit of SEA gene in low fat milk by PCR. Four strains of SEA-producing Staphylococcus aureus cultures were serially diluted in low fat milk. The SEA gene was detected at levels of 5.5 x 10(2) to 1.6 x 10(4) cfu/mL of S. aureus. These amounts of S. aureus are higher than the values in raw milk reported previously. Therefore we consider that SE genes in low fat milk should usually be undetectable by our PCR. This study shows that quick detection of SE genes by PCR is very helpful to analyze outbreaks, especially if no significant bacterium can be cultured. 相似文献
90.
Kobayashi M Ohtsuka K Tamura Y Tomizawa S Kamijo K Iwakoshi K Kageyama Y Nagayama T Takano I 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2011,52(2):121-129
A survey of pesticide residues in 595 imported frozen products on the Tokyo market from April 1989 to March 2008 was carried out. Forty three kinds of pesticides, including organophosphorus, organochlorine, carbamate, pyrethroid and others, were detected between levels of trace (below 0.01 ppm) and 4.6 ppm from 162 samples. Chlorpyrifos, cypermethrin and omethoate were frequently detected in green vegetables (komatsuna leaf and spinach), cypermethrin and methamidophos were detected in pods and seeds (green soybean and string pea), chlorpropham (CIPC) was detected in potato, and captan and carbaryl were detected in berries (blueberry, raspberry and strawberry). The hydrophilic pesticide methamidophos was detected in flesh of lychee. Residue levels of these pesticides were calculated as between less than 0.5% and 30% of their ADI values according to the daily intake of frozen products. Therefore, these frozen products should be safe when they were eaten in customary amounts. 相似文献