全文获取类型
收费全文 | 1533篇 |
免费 | 18篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 65篇 |
综合类 | 3篇 |
化学工业 | 247篇 |
金属工艺 | 35篇 |
机械仪表 | 20篇 |
建筑科学 | 18篇 |
能源动力 | 25篇 |
轻工业 | 97篇 |
水利工程 | 1篇 |
无线电 | 255篇 |
一般工业技术 | 273篇 |
冶金工业 | 386篇 |
原子能技术 | 52篇 |
自动化技术 | 75篇 |
出版年
2023年 | 8篇 |
2022年 | 9篇 |
2021年 | 20篇 |
2020年 | 6篇 |
2018年 | 11篇 |
2017年 | 14篇 |
2016年 | 20篇 |
2015年 | 8篇 |
2014年 | 24篇 |
2013年 | 35篇 |
2012年 | 37篇 |
2011年 | 42篇 |
2010年 | 38篇 |
2009年 | 35篇 |
2008年 | 65篇 |
2007年 | 41篇 |
2006年 | 49篇 |
2005年 | 47篇 |
2004年 | 43篇 |
2003年 | 39篇 |
2002年 | 47篇 |
2001年 | 37篇 |
2000年 | 28篇 |
1999年 | 53篇 |
1998年 | 134篇 |
1997年 | 101篇 |
1996年 | 70篇 |
1995年 | 54篇 |
1994年 | 25篇 |
1993年 | 38篇 |
1992年 | 30篇 |
1991年 | 21篇 |
1990年 | 26篇 |
1989年 | 25篇 |
1988年 | 17篇 |
1987年 | 20篇 |
1986年 | 24篇 |
1985年 | 31篇 |
1984年 | 15篇 |
1983年 | 16篇 |
1982年 | 17篇 |
1981年 | 14篇 |
1980年 | 19篇 |
1979年 | 18篇 |
1978年 | 9篇 |
1977年 | 20篇 |
1976年 | 24篇 |
1975年 | 6篇 |
1974年 | 6篇 |
1973年 | 5篇 |
排序方式: 共有1552条查询结果,搜索用时 0 毫秒
61.
Masatoshi Sakairi Fumihito Sato Yoshihito Gotou Koji Fushimi Tatsuya Kikuchi Hideaki Takahashi 《Electrochimica acta》2008,54(2):616-622
A new method for maskless fabrication of metallic patterns or structures on metals is described. A solution flow type droplet cell, with co-axial dual capillaries was applied to form fine metal structures such as strips and rods. This type of droplet cell enables movement of the cell during formation. Nickel fine patterns with a width of about 200 μm were formed on a Cu substrate. The width of the formed pattern does not change with the scanning speed of the cell, but the thickness of the formed pattern changes with the speed. Two different deposition modes were examined to form metal rods, one is a mold free deposition mode and the other is a mold assisted deposition mode. Both modes enable the formation of Ni rods, however, reproducibility of mold free deposition mode was not good. The mold assisted deposition mode has far better the reproducibility, because of the use of the inside wall of the 100 μm diameter inner tube as the mold. It is possible to form nickel micro-rods, about 100 μm in diameter and 12 mm long with relatively smooth surfaces by the mold assisted deposition mode. 相似文献
62.
Saito Kouhei; Kikuchi Takeshi; Yoshida Michiteru 《Protein engineering, design & selection : PEDS》1999,12(3):235-242
The DNA binding mechanism of box B in HMG1, a member of thesequence non-specific DNA binding HMG1/2-box family of proteins,has been examined by both mutation analyses and molecular modelingtechniques. Substitution of the residue 102F, which is characteristicallyexposed to solvent, with a small hydrophobic amino acid affectedits DNA binding activity. However, no additional effect wasobserved by the further mutation of flanking 101F. Moleculardynamics simulation and modeling studies revealed that 102Fintercalates into DNA base-pairs, being supported by the flanking101F. The mutants with a small hydrophobic residue at position102 tolerated the substitution for 101F because the side chainat position 102 is too short to intercalate. Thus the intercalationof 102F and the positive effect of the flanking 101F residueare important for the sequence non-specific DNA binding of theHMG1/2-box. The conserved basic residues of 95K, 96R and 109Rwere also examined for their roles in DNA binding. These residuesinteracted with DNA mainly by electrostatic interaction andmaintained the location of the box on the DNA, which prescribedthe intercalation of 102F. The DNA intercalation by HMG1 consistsof an ingenious mechanism which brings DNA conformational changesnecessary for biological functions. 相似文献
63.
64.
Que Dan Nguyen Kosuke Kikuchi Basudev Maity Takafumi Ueno 《International journal of molecular sciences》2021,22(4)
Protein assemblies provide unique structural features which make them useful as carrier molecules in biomedical and chemical science. Protein assemblies can accommodate a variety of organic, inorganic and biological molecules such as small proteins and peptides and have been used in development of subunit vaccines via display parts of viral pathogens or antigens. Such subunit vaccines are much safer than traditional vaccines based on inactivated pathogens which are more likely to produce side-effects. Therefore, to tackle a pandemic and rapidly produce safer and more effective subunit vaccines based on protein assemblies, it is necessary to understand the basic structural features which drive protein self-assembly and functionalization of portions of pathogens. This review highlights recent developments and future perspectives in production of non-viral protein assemblies with essential structural features of subunit vaccines. 相似文献
65.
Fuminori Tanihara Maki Hirata Nhien Thi Nguyen Osamu Sawamoto Takeshi Kikuchi Takeshige Otoi 《International journal of molecular sciences》2021,22(5)
Xenoantigens cause hyperacute rejection and limit the success of interspecific xenografts. Therefore, genes involved in xenoantigen biosynthesis, such as GGTA1, CMAH, and B4GALNT2, are key targets to improve the outcomes of xenotransplantation. In this study, we introduced a CRISPR/Cas9 system simultaneously targeting GGTA1, CMAH, and B4GALNT2 into in vitro-fertilized zygotes using electroporation for the one-step generation of multiple gene-edited pigs without xenoantigens. First, we optimized the combination of guide RNAs (gRNAs) targeting GGTA1 and CMAH with respect to gene editing efficiency in zygotes, and transferred electroporated embryos with the optimized gRNAs and Cas9 into recipient gilts. Next, we optimized the Cas9 protein concentration with respect to the gene editing efficiency when GGTA1, CMAH, and B4GALNT2 were targeted simultaneously, and generated gene-edited pigs using the optimized conditions. We achieved the one-step generation of GGTA1/CMAH double-edited pigs and GGTA1/CMAH/B4GALNT2 triple-edited pigs. Immunohistological analyses demonstrated the downregulation of xenoantigens; however, these multiple gene-edited pigs were genetic mosaics that failed to knock out some xenoantigens. Although mosaicism should be resolved, the electroporation technique could become a primary method for the one-step generation of multiple gene modifications in pigs aimed at improving pig-to-human xenotransplantation. 相似文献
66.
H. Sabet K. Ishii S. Matsuyama Y. Kikuchi K. Nakazawa A. Esmaili Torshabi H. Yamazaki 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》2009,600(3):678-682
A new method to modify coordinates of detectors in any positron emission tomography (PET) system using a radioactive point source is developed. This method is based on selecting a centered detector in each detector block of PET and measuring coincidence counts between the two centered detectors in opposite detector blocks to find the coordinates of their LOR (Line of Response). Due to slight misalignment of detector positions, measured LORs may not intersect at a single point. Based on the proposed method, the coordinates of detectors can be measured with very high accuracy and the coordinate of the center of the gantry (which is normally the same as the center of field of view) can be defined correctly. The results of the application of our method to a small animal PET system (FinePET), which was recently developed at Tohoku University, Japan, are shown here. The method is expected to contribute to the design and development of PET systems which can realize a very high spatial resolution of less than 1 mm FWHM. 相似文献
67.
Yasushi Sekine Haruka Takamatsu Sho Aramaki Kazuki Ichishima Mitsuko Takada Masahiko Matsukata Eiichi Kikuchi 《Applied Catalysis A: General》2009,352(1-2):214-222
The water gas shift (WGS) reaction over Pt and Pd catalysts supported on various perovskite oxides has been investigated at 573 K without catalyst pretreatment. The Pt and Pd catalysts on LaCoO3 support showed high catalytic activity. Interaction between Pt or Pd and the support is considered to promote the WGS reaction: Pt/LaCoO3 had high initial activity but deactivated immediately; Pd/LaCoO3 was less active than Pt/LaCoO3, but had superior stability. Catalysts were characterized using XRD, STEM, XPS, and H2-temperature programmed reduction (TPR). Results of this study showed that reduction of the support decreased the CO conversion on Pt/LaCoO3. On the other hand, Pd/LaCoO3 showed stable activity for the WGS reaction. Therefore, Pd was added to Pt/LaCoO3 for stabilizing the catalyst activity, and 0.5 wt.% Pd/1 wt.% Pt/LaCoO3 catalyst showed higher activity and stability. 相似文献
68.
Matsui T Matsumura K Hagisawa K Ishihara M Ishizuka T Suzuki M Kurita A Kikuchi M 《IEEE transactions on bio-medical engineering》2002,49(6):621-623
The purpose of this study is to investigate the vascular wall with a thermally self-regulating, cylindrical stent made of a low Curie temperature ferromagnetic alloy. Physiologic saline was circulated in the silicone model vessel implanted with the stent. The stent-temperature remained nearly constant for variable saline flows, saline temperatures, and magnetic flux densities. Stent implants of this type in human blood vessels could potentially enable thermotherapy and temperature determination without catheterization. 相似文献
69.
Steroid cyclophanes, each having a macrocyclic ring attached to four bile acid moieties via chiral lysine connectors, were synthesized, and the binding of the 2-naphthylphenylketone (guest) to the steroid cyclophanes in water was investigated. The circular dichroism spectra of the steroid cyclophane with cholic acid and L-lysine were significantly affected by the binding of the guest, and the induced circular dichroism based on the absorption of the achiral guest was also observed. The binding of the guest to the steroid cyclophane with cholic acids and D-lysines induced changes in the circular dichroism spectra with the opposite sign of the molecular ellipticities. An induced circular dichroism spectral change was not observed upon binding of the guest to the analogous host without OH sites. These results strongly suggest that the guest is conformationally fixed through hydrogen bonding between the carbonyl group of the guest and the steroidal hydroxyl group of the host. The assembly of only four steroid residues on the macrocyclic ring probably provided a hydrophobic nanocavity for hydrogen bonding. 相似文献
70.
The effect of the dispersing procedure on the aggregate size, membrane fluidity and the pharmacokinetics were evaluated for the lipid A analog E5531. The size of the aggregates prepared by the pH-jump method (pH 11.0 → 7.3) was decreased, reaching 20 nm with increasing dispersing time in 0.003 N NaOH (pH 11.0). The membrane fluidity of the aggregates increased with increasing dispersing time. When prepared by the normal dilution method (pH 7.3 → 7.3), the size of the aggregates remained constant at 150 nm and the membrane fluidity was smaller compared to samples prepared by the pH-jump method. Using samples with different degrees of hydration and different membrane fluidities prepared by the pH-jump method, the pharmacokinetics after intravenous administration into rats were evaluated, and the data obtained confirmed that the membrane fluidity was correlated with the pharmacokinetics in rat. In addition, E5531 vials were stable for 24 months at room temperature when used within 24 hr after reconstitution. 相似文献