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11.
In this study, a new marine oil that contains 45% docosahexaenoic acid (DHA, 22∶6n−3) and 13% docosapentaenoic acid (DPA,
22∶5n−6) was administered to rats. The metabolism and distribution of DPA in rats was investigated. In experiment 1, the effects
of DHA and n−6 fatty acids (linoleic acid, I A; arachidonic acid, AA; and DPA) on AA contents were investigated in vivo. LA group: LA 25%, DHA 30%; LA-DPA group: LA 15%, DPA 10%, DHA 35%; LA-AA-DPA group: LA 10%, AA 5%, DPA 10%, DHA 35% were
administered to rats for 4 wk. In the liver, the AA content in the LA-DPA and LA-AA-DPA groups was significantly higher than
in the LA group. The decreased AA contents in the LA group might be caused by DHA administration. Although DHA also was administered
in the LA-DPA and LA-AA-DPA groups, the AA contents in these two groups did not decrease. These results suggested that DPA
retroconverted to AA, blunting the decrease in AA content caused by DHA administration. To conduct a detailed investigation
on DPA metabolism and its relation with AA and DHA, rat hepatocytes were cultured with pruified DPA and DHA for 24 h. We discovered
the retroconversion of DPA to AA occurred only when AA content was decreased by a high DHA administration; it did not occur
when AA content was maintained at a normal level. 相似文献
12.
Shimizu Sakayu Akimoto Kengo Kawashima Hiroshi Shinmen Yoshifumi Yamada Hideaki 《Journal of the American Oil Chemists' Society》1989,66(2):237-241
The mycelial dihomo-γ-linolenic acid content of an arachidonic acid-producing fungus,Mortierella alpina 1S-4, was found to increase, with an accompanying marked decrease in its arachidonic acid content, on cultivation with sesame
oil. The resultant mycelia were found to be a rich source of dihomo-γ-linolenic acid. This unique phenomenon was suggested
to be due to specific repression of the conversion of dihomo-γ-linolenic acid to arachidonic acid by the oil. After fractionation
of the oil with acetone into oil and non-oil fractions, it was found that the effective factor(s) was present in the non-oil
fraction. In a study on optimization of the culture conditions for the production of dihomo-γ-linolenic acid byM. alpina 1S-4, a medium containing glucose, yeast extract and the non-oil fraction was found to be suitable for the production. Under
the optimal conditions in a 50-1 fermentor, the fungus produced 107 mg of dihomo-γ-linolenic acid/g dry mycelia (2.17 g/l
of culture broth). This value accounted for 23.1% of the total fatty acids in the lipids extracted from the mycelia. The mycelia
were also rich in arachidonic acid (53.5 mg/g dry mycelia, 11.2%). Other major fatty acids in the lipids were palmitic acid
(24.1%), stearic acid (7.0), oleic acid (20.1), linoleic acid (6.6) and γ-linolenic acid (4.1).
On leave from Suntory Ltd. 相似文献
13.
[This corrects the article on p. 1930 in vol. 11, PMID: 20480050.]. 相似文献
14.
Kinoshita Kengo; Sadanami Keishi; Kidera Akinori; Go Nobuhiro 《Protein engineering, design & selection : PEDS》1999,12(1):11-14
In order to search for a common structural motif in the phosphate-bindingsites of proteinmononucleotide complexes, we investigatedthe structural variety of phosphate-binding schemes by an all-against-allcomparison of 491 binding sites found in the Protein Data Bank.We found four frequently occurring structural motifs composedof protein atoms interacting with phosphate groups, each ofwhich appears in different protein superfamilies with differentfolds. The most frequently occurring motif, which we call thestructural P-loop, is shared by 13 superfamilies and is characterizedby a four-residue fragment, GXXX, interacting with a phosphategroup through the backbone atoms. Various sequence motifs, includingWalker's A motif or the P-loop, turn out to be a structuralP-loop found in a few specific superfamilies. The other threemotifs are found in pairs of superfamilies: protein kinase andglutathione synthetase ATPase domain like, actin-like ATPasedomain and nucleotidyltransferase, and FMN-linked oxidoreductaseand PRTase. 相似文献
15.
Summary Steel fibre reinforced metal matrix composites (FRM) consisting of high‐strength ductile steel wire and aluminium or titanium foil were fabricated by explosive bonding. The strength properties were evaluated by tensile tests. The optimum explosive loading (explosive mass/driver plate mass) increases with the volume fraction of fibre. For the aluminium matrix composites, the micrographs obtained show sound bonding between the adjoining foils and non‐bonding between the foil and steel wire. The tensile strength of the aluminium matrix composites is 80% of the value predicted by the rule of mixtures. For the titanium matrix composites, the micrographs obtained show both bonded and unbonded regions between the adjoining foils or between the foil and steel wire. The tensile strength of the titanium matrix composites agrees with the value predicted by the rule of mixtures. 相似文献
16.
The programmability of RNA–RNA interactions through intermolecular base-pairing has been successfully exploited to design a variety of RNA devices that artificially regulate gene expression. An in silico design for interacting structured RNA sequences that satisfies multiple design criteria becomes a complex multi-objective problem. Although multi-objective optimization is a powerful technique that explores a vast solution space without empirical weights between design objectives, to date, no web service for multi-objective design of RNA switches that utilizes RNA–RNA interaction has been proposed. We developed a web server, which is based on a multi-objective design algorithm called MODENA, to design two interacting RNAs that form a complex in silico. By predicting the secondary structures with RactIP during the design process, we can design RNAs that form a joint secondary structure with an external pseudoknot. The energy barrier upon the complex formation is modeled by an interaction seed that is optimized in the design algorithm. We benchmarked the RNA switch design approaches (MODENA+RactIP and MODENA+RNAcofold) for the target structures based on natural RNA-RNA interactions. As a result, MODENA+RactIP showed high design performance for the benchmark datasets. 相似文献
17.
Matsumura K Obata H Hata Y Kawato A Abe Y Akita O 《Journal of Bioscience and Bioengineering》2004,98(2):77-84
We cloned and characterized a novel gene (abfA) encoding alpha-L-arabinofuranosidase (alpha-L-AFase) from Aspergillus oryzae. One clone homologous to the alpha-L-AFase gene of Thermotoga maritima was found in an expressed sequence tag (EST) library of A. oryzae and a corresponding gene was isolated. Molecular analysis showed that the abfA gene carried six exons interrupted by five introns and had an open reading frame encoding 481 amino acid residues. The amino acid sequence similarity at active sites to the alpha-L-AFases from other organisms indicated that the alpha-L-AFase encoded by abfA was classified as a family 51 glycoside hydrolase. When the abfA was overexpressed in the homologous hyperexpression system of A. oryzae, a large amount of alpha-L-AFase was produced as intracellular protein. The apparent molecular mass of the purified enzyme was estimated to be 228,000 by gel filtration and that of its subunit as 55,000 by SDS-PAGE, suggesting that the enzyme is a tetramer. The enzyme hydrolyzed p-nitrophenyl-alpha-L-arabinofuranoside but not other p-nitrophenyl glycosides. These results demonstrated that the abfA gene encodes a functional alpha-L-AFase. 相似文献
18.
Yoshihiro Hishikawa Haitao Liu Hsin‐Hsin Hsieh Takamitsu Inoue Kyung‐Soo Kim Chamnan Limsakul Seong‐Won Kim Ruengrit Ninae Kengo Morita 《Progress in Photovoltaics: Research and Applications》2013,21(5):1181-1188
Because of recent advances in the production and installation of photovoltaic (PV) systems, the international conformity of PV module performance measurement has become increasingly important. The increase in PV production sites is particularly significant in the Asian region. The present paper summarizes and discusses the results of a round‐robin intercomparison of crystalline silicon modules among national laboratories and certified testing laboratories in the Asian region conducted from 2009 to 2011. Most of the values of Pmax measured at the different laboratories were within a ±2% range, although some Pmax results showed differences of up to about 3%. This result is comparable to that obtained in the recent intercomparison among international laboratories. Possible sources of difference in the measured values of Isc, Voc, FF, and Pmax are discussed, for further improvement of international conformity in PV measurement technologies. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
19.
Sakayu Shimizu Kengo Akimoto Yoshifumi Shinmen Hiroshi Kawashima Michihiro Sugano Hideaki Yamada 《Lipids》1991,26(7):512-516
Incubation with sesame oil increases the mycelial dihomo-γ-linolenic acid content of an arachidonic acid-producing fungus,Mortierella alpina, but decreases its arachidonic acid content [Shimizu, S., K. Akimoto, H. Kawashima, Y. Shinmen and H. Yamada (1989)J. Am. Oil Chem. Soc. 66, 237–241]. The factor causing these effects was isolated and identified to be (+)-sesamin. The results obtained in experiments
with both a cell-free extract of the fungus and with rat liver microsomes demonstrated that (+)-sesamin specifically inhibits
Δ5 desaturase at low concentrations, but does not inhibit Δ6, Δ9 and Δ12 desaturases. Kinetic analysis showed that (+)-sesamin
is a noncompetitive inhibitor (Ki for rat liver Δ5 desaturase, 155 μM). (+)-Sesamolin, (+)-sesaminol and (+)-episesamin, also inhibited only Δ5 desaturases
of the fungus and liver. These results demonstrate that (+)-sesamin and related lignan compounds present in sesame seeds or
its oil are specific inhibitors of Δ5 desaturase in polyunsaturated fatty acid biosynthesis in both microorganisms and animals.
On leave from Suntory Ltd. 相似文献
20.
Kengo Akaho Takashi Nakagawa Yoshihisa Yamaguchi Katsuya Kawai Hirokazu Kato Shogo Nishida 《Electrical Engineering in Japan》2012,180(2):43-54
Car navigation systems play a prominent role in road traffic safety and traffic regulation. However, it is necessary to improve the route guidance of car navigation systems so that they accurately and quickly recognize small differences in location. Thus, to increase the ease of understanding and safety of car navigation systems, navigation systems based on augmented reality have been proposed for providing guidance at road intersections. We are currently developing a car navigation system based on augmented reality, called AR‐Navi. We investigated designs for the display of road intersection guidance that can be easily understood even when limited information is available and proposed a “best shot” display method that does not use moving images. In addition, we implemented a prototype system that includes these methods and conducted driving experiments on public roads to evaluate the ease of understanding and safety of AR‐Navi. Using the evaluation results, we confirmed that the ease of understanding and safety is similar in the case of AR‐Navi and CG‐Navi. We also clarified the characteristics of AR‐Navi. © 2012 Wiley Periodicals, Inc. Electr Eng Jpn, 180(2): 43–54, 2012; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/eej.22278 相似文献