A cytoplasmic xylanase (XynX) of Aeromonas caviae ME-1 is released from the cytoplasm to the periplasm by osmotic downshock

Aeromonas caviae ME-1 is a multiple xylanase-producing gram-negative bacterium which was isolated from the gut contents of a wild silkworm, Samia cynthia pryeri. One of the xylanases produced by A. caviae ME-1, XynX (38 kDa, family 10 xylanase), hydrolyzes xylan to xylobiose and xylotetraose as final degradation products. Generally, xylanases are extracellular or cell surface enzymes. However, XynX is not exported to the extracellular fluid by A. caviae ME-1 and an Escherichia coli transformant harboring the xynX gene. In this study, we investigated the intracellular localization of XynX in A. caviae ME-1 and an E. coli transformant. XynX was found in the cytoplasm when the cells were grown under normal culture conditions. However, XynX was released from the cytoplasm to the periplasm during osmotic downshock. This release of XynX in the E. coli transformant was blocked in the presence of gadolinium chloride, which has been reported to be an inhibitor of bacterial mechanosensitive channels.     

Multilayer Construction with Various Ceramic Films for Electronic Devices Fabricated by Aerosol Deposition

Aerosol deposition (AD) is applicable as a fabrication technology for microstructures comprising different materials. We used this method for electronic devices that consist of ceramic films and metal electrodes. Various ceramic thick films (5–50 μm thickness), for example, Al₂O₃, 2MgO·SiO₂, and BaTiO₃, were deposited on substrates using room-temperature aerosol deposition. The dielectric constant of BaTiO₃ was 78 at 1 MHz. Multilayer constructions with ceramic films and copper electrodes were obtained using aerosol deposition and sputtering. During deposition, photoresist film masks were applied to produce patterns of ceramic films and connections between upper and lower electrodes through the ceramic films.     

Shear behaviour of methane hydrate bearing sand with various particle characteristics and fines

It is recognized that methane hydrate (hereafter referred to as MH) is trapped in the sand sediments of alternating sand and mud layers in the turbidite of the Nankai Trough, Japan. The existence of fines within the marine sediment significantly affects its mechanical and physical properties. A series of plane strain compression tests at high pressures were performed in order to investigate the effect of the particle characteristics and fines content of the host sands on the shear behaviour of MH bearing sands. MH bearing sands were artificially produced using rounded glass beads and three other silica sands with different fines contents. A high-pressure low-temperature testing apparatus was equipped with a camera to observe deformation of the specimens during shearing and particle image velocimetry analysis was conducted on pictures taken during the experiments. The experimental results show that strength enhancement due to the bonding effect in MH bearing sand increases with the level of fines content. Values for both the cohesion and friction angle of MH bearing sand composed of Toyoura sand increased along with increasing MH saturation. However, in the case of MH bearing glass beads, only the value for cohesion increased when MH was formed. The maximum shear strain of MH bearing glass beads was mostly concentrated near the shear band. While the maximum shear strains of the three other MH bearing sands were concentrated within the shear band, some was widely distributed in the region outside of the shear band. A rise in the degree of MH saturation increased the angle and narrowed the width of the shear band, regardless of the fines content.     

Study on Specific Modification of Glucosyl Cyclodextrins

The time courses of the periodate oxidation of glucosyl cyclodextrins complexed with organic solvents indicated the preferential oxidation of glucosyl residues on side chains, and the modified cyclodextrins could be purified by using a reversed phase column. HPLC and ¹³C-NMR analyses ensured that the modified cyclodextrins were cyclodextrins having aldehyde residues on their side chains (aldehyde cyclodextrins).