Co-enzyme specificity of 3-isopropylmalate dehydrogenase from Thermits thermophilus HB8

The co–enzyme specificity of 3–isopropylmalate dehydrogenasefrom an extreme thermophile, Thermus thermophilus HB8, was changedfrom NAD to NADP by site–directed mutagenesis Based onsequence comparison of 3–isopropylmalate dehydrogenasesfrom various organisms with NAD– and NADP–dependentisocitrate dehydrogenases, Ser226, Ser253 and De279 of 3–isopropylmalatedehydrogenase were suggested as determining the co–enzymespecificity. These residues were replaced with the correspondingresidues of NADP–dependent isocitrate dehydrogenases;Arg, Gly and Tyr respectively. The single–mutated enzymes,S226R and I279Y, enhanced the activities towards NADP 10–and 3–fold respectively, whereas S253G reduced the activity.Among the multiple–mutated enzymes, the double–mutatedS226R/I279Y increased the catalytic efficiency against NADP( fold) and shifted the specificity for NAD towards NADP mostsignificantly ( 173–fold).     

Clinical application of single-shot echo-planar diffusion-weighted imaging with compressed SENSE in prostate MRI at 3T: preliminary experience

Magnetic Resonance Materials in Physics, Biology and Medicine - Image quality (IQ) of diffusion-weighted imaging (DWI) with single-shot echo-planar imaging (ssEPI) suffers from low signal-to-noise...     

Development of a pulse control-type MEMS microrobot with a hardware neural network

This article presents the micro-electro-mechanical systems (MEMS) microrobot which demonstrates locomotion controlled by hardware neural networks (HNN). The size of the microrobot fabricated by the MEMS technology is 4 × 4 × 3.5 mm. The frame of the robot is made of silicon wafer, and it is equipped with a rotary-type actuator, a link mechanism, and six legs. The rotary-type actuator generates rotational movement by applying an electrical current to artificial muscle wires. The locomotion of the microrobot is obtained by the rotation of the rotary-type actuator. As in a living organism, the HNN realized robot control without using any software programs, A/D converters, or additional driving circuits. A central pattern generator (CPG) model was implemented as an HNN system to emulate the locomotion pattern. The MEMS microrobot emulated the locomotion method and the neural networks of an insect with the rotary-type actuator, the link mechanism, and the HNN. The microrobot performed forward and backward locomotion, and also changed direction by inputting an external trigger pulse. The locomotion speed was 0.325 mm/s and the step width was 1.3 mm.     

Spin Injection Processes in ZnSe-Based Double Quantum Dots of Diluted Magnetic Semiconductors

Spin injection processes in the double quantum dots of ZnSe-based diluted magnetic semiconductors are discussed. Double quantum dots are fabricated from ZnSe-based double quantum wells by electron beam lithography and wet etching. In these samples, the photo-excited carriers in the magnetic dots are injected into the non-magnetic dots. The circular polarization degrees of photoluminescence from the non-magnetic dots are measured by micro-photoluminescence measurement system under the magnetic field up to 5 T. The maximum spin polarization degrees of injected carriers determined from our experiment are 10% for double quantum wells and 15% for double quantum dots. The spin injection efficiency was estimated both from the observed circular polarization degree and the diffusion length of carriers. We concluded that the spin injection efficiency is increased in the double quantum dots.     

Stress distribution of coated film with a range of coated film thickness and elastic properties under a single EHL operating condition

In recent years, the techniques improving sliding performances have progressed by using coated films possessing superior tribological properties, to reduce the failures of the mechanical elements. Those techniques are often used under severe conditions such as elastohydrodynamic lubrication (EHL). In this paper, numerical three-dimensional analysis of the maximum shear stress applied into the coated film and substrate under a single EHL operating condition was performed with a range of coated film thickness and elastic properties. The strength of coated film as one of those techniques was evaluated numerically, resulting in an optimum design of coated film. As a result, coated films with a larger value of thickness and a smaller modulus of elasticity than that of substrate are preferable.     

Characteristics of the Arc Voltage of a High‐Current Air Arc in a Sealed Chamber

The effect of the arc voltage on various factors of design and control was investigated for high currents in order to develop design guidelines for circuit breakers. In this study, the dependence on such factors, namely, the current, arc length, electrode surface area, and internal pressure of the arc voltage, was evaluated quantitatively. As a result of the evaluations, it was estimated that the arc voltage near the electrode surface rises linearly with the arc current and the power ?0.8 of the surface area, and that the voltage in the arc column rises as the 0.3 power of the pressure increase. We confirmed the validity of the estimated voltage characteristics by comparison with the generated voltage in an actual arc‐extinction chamber. The characteristics of the estimated voltage can provide effective guidelines for the design of arc extinguishing chambers. © 2013 Wiley Periodicals, Inc. Electr Eng Jpn, 186(1): 34–42, 2014; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.22487