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101.
102.
The rapid acquisition and subsequent retention of lithium-induced conditioned changes in taste reactivity responses to sucrose were examined in rats with the area postrema (AP) either ablated or intact. On 2 conditioning days, a series of brief intraoral sucrose infusions was paired with the effects of LiCl or NaCl injections. Repeated associations of the sucrose taste with the effects of lithium significantly reduced ingestive responses and increased aversive responses only in the AP-intact group. AP-ablated rats treated with LiCl and rats injected with NaCl displayed an ingestive pattern of responses. Only the AP-intact rats, previously injected with LiCl, subsequently displayed evidence of a conditioned taste aversion. We conclude that toxin activation of the AP is required to produce the conditioned shift in taste reactivity responses and subsequent expression of a taste aversion in rats treated with lithium. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
103.
VA Maltsev HN Sabbah M Tanimura M Lesch S Goldstein AI Undrovinas 《Canadian Metallurgical Quarterly》1998,54(6):597-605
Abnormalities of contractile function have been identified in cardiomyocytes isolated from failed human hearts and from hearts of animals with experimentally induced heart failure (HF). The mechanism(s) responsible for these functional abnormalities are not fully understood. In the present study, we examined the relationship between action potential duration, pattern of contraction and relaxation, and associated intracellular Ca2+ transients in single cardiomyocytes isolated from the left ventricle (LV) of dogs (n = 7) with HF produced by multiple sequential intracoronary microembolizations. Comparisons were made with LV cardiomyocytes isolated from normal dogs. Action potentials were measured in isolated LV cardiomyocytes by perforated patch clamp, Ca2+ transients by fluo 3 probe fluorescence, and cardiomyocyte contraction and relaxation by edge movement detector. HF cardiomyocytes exhibited an abnormal pattern of contraction and relaxation characterized by an attenuated initial twitch (spike) followed by a sustained contracture ('dome') of 1 to 8 s in duration and subsequent delayed relaxation. This pattern was more prominent at low stimulation rates (58% at 0.2 Hz, n = 211, 21% at 0.5 Hz, n = 185). Measurements of Ca2+ transients in HF cardiomyocytes at 0.2 Hz manifested a similar spike and dome configuration. The dome phase of both the contraction/relaxation pattern and Ca2+ transients seen in HF cardiomyocytes coincided with a sustained plateau of the action potential. Shortening of the action potential duration by administration of saxitoxin (100 nM) or lidocaine (30 microM) reduced the duration of the dome phase of both the contraction/relaxation profile as well as that of the Ca2+ transient profile. An increase of stimulation rate up to 1 Hz caused shortening of the action potential and disappearance of the spike-dome profile in the majority of HF cardiomyocytes. In HF cardiomyocytes, the action potential and Ca2+ transient duration were not significantly different from those measured in normal cells. However, the contraction-relaxation cycle was significantly longer in HF cells (314 +/- 67 ms, n = 21, vs. 221 +/- 38 ms, n = 46, mean +/- SD), indicating impaired excitation-contraction uncoupling in HF cardiomyocytes. The results show that, in cardiomyocytes isolated from dogs with HF, contractile abnormalities and abnormalities of intracellular Ca2+ transients at low stimulation rates are characterized by a spike-dome configuration. This abnormal pattern appears to result from prolongation of the action potential. 相似文献
104.
105.
N. Sutivisedsak H.N. Cheng J.L. Willett W.C. Lesch R.R. Tangsrud Atanu Biswas 《Food research international (Ottawa, Ont.)》2010,43(2):516-519
Phenolic phytochemicals are associated with many health benefits, and it would be useful to develop improved methods for their extraction from bean. In this work, we showed that microwave-assisted extraction was an effective method. Eight bean types important in the Northarvest region of North America, which includes North Dakota and Minnesota, were chosen for this study. Four temperatures (25, 50, 100 and 150 °C) and three solvents (water, 50% ethanol in water and 100% ethanol) were investigated. As expected, colored beans contained higher concentrations of extractable phenolics, and in most cases the concentration of phenolics was much higher in the hull (testa) than in the cotyledon. Extraction efficiency was superior at higher extraction temperatures. The most effective extraction was achieved at a temperature of 150 °C using 50% ethanol. Total phenolics contents determined by microwave-assisted extraction with water at 100 °C were two to three times those determined by conventional extraction with water at the same temperature. 相似文献
106.
Today, drivers are faced with many in-vehicle activities that are potentially distracting. In many cases, they are not passive recipients of these tasks; rather, drivers decide whether or not (or how) to perform them. In this study, we examined whether drivers, given knowledge of the upcoming road demands, would strategically delay performing in-vehicle activities until demands were reduced. Twenty drivers drove an instrumented van around a closed track that was divided into sections of varying demands and difficulty. Drivers were asked to perform one of four in-vehicle tasks (e.g., phone conversation; read a text message; find an address; pick up an object on the floor); however, they were free to decide when to initiate these tasks, provided they finish them before a given deadline. Although drivers were fully aware of the relative demands of the road, they did not tend to strategically postpone tasks—a finding that was consistent across the different tasks (p > .05). Rather, drivers tended to initiate tasks regardless of the current driving conditions. This strategy frequently led to driving errors. Given the control that drivers have over many in-vehicle distractions, interventions that focus on strategic decisions and planning may have merit. 相似文献
107.
Cortés-Salazar F Momotenko D Girault HH Lesch A Wittstock G 《Analytical chemistry》2011,83(5):1493-1499
Specialized microelectrode probes fabricated in a soft polymer film now make it possible to use scanning electrochemical microscopy to image the reactivity of large, corrugated, tilted, and dry surfaces. (To listen to a podcast about this Feature, please go to the Analytical Chemistry multimedia page at pubs.acs.org/page/ancham/audio/index.html .). 相似文献
108.
Claudia Lurtz Karsten Voss Veronika Hahn Frieder Schauer Juergen Wegmann Erich K. Odermatt Klaus-Peter Schmitz Katrin Sternberg 《Journal of materials science. Materials in medicine》2013,24(3):667-678
Biodegradability and adhesive-associated local drug release are important aspects of research in tissue adhesive development. Therefore, this study focuses on investigating the in vitro degradation and drug release of a tissue adhesive consisting of hexamethylene diisocyanate functionalized 1,2-ethylene glycol bis(dilactic acid) and chitosan chloride. To prevent infections, ciprofloxacin hydrochloride (CPX·HCl) was incorporated into the adhesive. The influence of CPX·HCl on the adhesive reaction and adhesive strength was analyzed by FTIR-ATR-spectroscopy and tensile tests. The CPX·HCl release was investigated by HPLC. The degradation-induced changes at 37 °C were evaluated by gravimetric/morphological analyzes and micro-computer tomography. The antibiotic potential of the CPX·HCl loaded adhesive was determined by agar diffusion tests. The degradation tests revealed a mass loss of about 78 % after 52 weeks. The adhesive reaction velocity and tensile strength were not influenced by CPX·HCl. Using a 2 mg/g CPX·HCl loaded adhesive an inhibition of all tested bacteria was observed. 相似文献
109.
Klaus-Peter Becker 《化学,工程师,技术》1979,51(6):549-559
The oxitron system: a new biological sewage treatment process for reducing BOD5 and for nitrification and denitrification . In the oxitron process, the waste water passes through a fluidized sand bed. As in the case of droplets, a stable biological slime is formed on the sand particles. The particular efficacy of the process can be explained in terms of the high specific surface, of the order of more than 3000 m2 per m3 reactor volume. This leads to a particularly effective use of available space. The high content of biomass makes the process largely insensitive to fluctuations in feed rates and toxic effects. The oxitron process is used as a biological purification stage for reducing BOD5 or as the third purification stage for nitrification and denitrification. The reactor can be operated at particularly high solids concentrations (ca. 20 g/l). The correspondingly high oxygen consumption of this process requires the use of pure oxygen in the aerobic step; over 90% of the oxygen supply is utilized. The special advantages of the oxitron process are: (1) 30% lower capital costs than for conventional processes (operating costs are comparable); (2) space requirements are some 80% lower than for the best conventional technology presently available, facilitating expansion; (3) the mode of operation is extremely simple and is not subject to interference by blockages, etc. Design data and results for large scale test plant are reported. 相似文献
110.
Jinggang Qin Klaus-Peter Weiss Yu Wu Guojun Liao Zhixiong Wu Laifeng Li Hongwei Li Erwu Niu 《Fusion Engineering and Design》2013,88(3):151-155
This paper focuses on mechanical tests on the ITER correction coils (CC) and Feeder jacket 316L stainless steel material. During manufacture, the conductor will be compacted and spooled after cable insertion. Therefore, sample jackets were prepared under compaction in order to simulate the status of conductor during manufacturing. Yield strength (0.2% offset), ultimate tensile strength, Young's modulus and elongation at failure shall be reported. The mechanical properties of materials were measured at 300 K and low temperature (<7 K). The cryogenic test results show that the present jackets have very high properties. It is concluded that the results meet the ITER requirement. 相似文献