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21.
Toshiyuki Shibata Ken Fujimoto Kohki Nagayama Kuniko Yamaguchi & Takashi Nakamura 《International Journal of Food Science & Technology》2002,37(6):703-709
The inhibitory effect of brown algal phlorotannins on hyaluronidase was evaluated by an in vitro assay. Crude phlorotannins from the brown algae Eisenia bicyclis and Ecklonia kurome had a stronger inhibitory effect than well-known inhibitors such as catechins and sodium cromoglycate. IC50 values of the following six phlorotannins: phloroglucinol, an unknown tetramer, eckol (a trimer), phlorofucofuroeckol A (a pentamer), dieckol and 8,8'-bieckol (hexamers), were 280, 650, >800, 140, 120 and 40 μ M , respectively. The IC50 of catechin, epigallocatechin gallate and sodium cromoglycate was 620, 190 and 270 μ M , respectively. 8,8'-Bieckol, the strongest HAase inhibitor in this study, acted as a competitive inhibitor with an inhibition constant ( K i ) of 35 μ M . Acetylation of the phlorotannins markedly decreased their inhibitory potency. 相似文献
22.
A high-field x-ray diffraction (HF-XRD) camera was developed to observe structural changes of magnetic materials in magnetic fields up to 10 T. The instrument mainly consists of a Debye-Scherrer-type camera with a diameter of 80.1 mm, a 10-T cryocooled superconducting magnet with a 100-mm room-temperature bore, an x-ray source, a power supply, and a chiller for the x-ray source. An x-ray detector (image plate) in the HF-XRD camera can be taken out and inserted into the magnet without changing the sample position. The performance of the instrument was tested by measuring the HF-XRD for silicon and ferromagnetic MnBi powders. A change of x-ray diffraction pattern was observed due to the magnetic orientation of MnBi, showing that the instrument is useful for studying field-induced orientation processes and structural properties of field-controlled materials. 相似文献
23.
Endoplasmic Reticulum (ER)‐Targeted,Galectin‐Mediated Retrograde Transport by Using a HaloTag Carrier Protein
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Dr. Sang‐Hyun Son Dr. Akira Seko Dr. Shusaku Daikoku Dr. Kohki Fujikawa Assoc. Prof. Dr. Katsuhiko Suzuki Chief Scientist Dr. Yukishige Ito Prof. Dr. Osamu Kanie 《Chembiochem : a European journal of chemical biology》2016,17(7):630-639
Investigations into metabolic processes within the cell have often relied on genetic methods such as forced expression and knockout or knockdown techniques. An alternative approach would be introducing a molecule into the desired location inside the cell. To translocate compounds from outside cells into the endoplasmic reticulum (ER), we constructed a delivery carrier protein. This comprised N‐terminal galectin‐1 for cell‐surface binding (G1), a protease cleavable sequence (ps), a HaloTag domain for attaching exogenous compounds (Halo), and a C‐terminal KDEL sequence for ER retention. Fluorescently labeled G1‐ps‐Halo‐KDEL passed through the Golgi apparatus and reached the ER. By using Man9GlcNAc2‐BODIPY as a cargo compound, the carrier protein was also delivered into the ER with concomitant processing of mannose to Man5,6, by the ER‐resident α1,2‐mannosidase. G1‐ps‐Halo‐KDEL might serve as a new type of delivery carrier protein to direct compounds into the ER. 相似文献
24.
Ray Hasegawa Hisao Fujiwara Hiroyuki Nagata Yujiro Hara Tatsuo Saishu Rieko Fukushima Masahiko Akiyama Haruhiko Okumura Kohki Takatoh 《Journal of the Society for Information Display》2001,9(2):107-113
Abstract— A 15‐in. TFT‐LCD with XGA resolution using thresholdless antiferroelectric liquid crystal (TLAF) has been developed. TLAF materials show V‐shaped switching and enable display of analog gray scale, wide viewing angle, and fast response. However, in the case that high‐resolution TFT‐LCDs using materials with large spontaneous polarization such as TLAF were driven by the conventional method, alternating current (ac) driving, the obtained contrast ratio was limited because of a sharp decline of holding voltage due to the growth of a depolarization field. In order to enhance the contrast ratio, a novel driving method referred to as quasi‐dc driving was proposed. In the quasi‐dc driving, the polarity of the applied voltage to liquid crystals inverts at certain intervals of several seconds. Moreover, the applied voltage and the charging time at the time of polarity inversion are increased more than the intended signals. By this method, the 15‐in. TFT‐LCD using TLAF with high contrast ratio (more than 100:1) and wide viewing angle was realized. 相似文献
25.
In situ FTIR studies showed the generation of a peroxide species by the contact of a H2-O2 gas mixture or of N2O with Fe-Al-P-O catalysts at 573 K. This oxygen species oxidized methane into methanol at 473 K, through the formation of methoxide species on the catalysts. 相似文献
26.
Takamasa Nonaka Yasuko Uemura Katsuto Ohse Kohki Jyono Seiji Kurihara 《应用聚合物科学杂志》1997,66(8):1621-1630
Copolymer beads (RCCS-4G) with many chloromethyl groups were prepared by treating macroreticular chloromethylstyrene-tetraethyleneglycol dimethacrylate (4G) copolymer beads with chloromethylether. Copolymer beads (RAAS-4G) with benzylamino groups were prepared by treating RCCS-4G with potassium phthalimide. Then the copolymer beads containing phenol derivatives were prepared by treating RAAS-4G with p-hydroxybenzoic acid (pHBA), 2,4-dihydroxybenzoic acid (DHBA), and 3,4,5-trihydroxybenzoic acid (gallic acid, GA) in N,N-dimethylformamide. The antibacterial activity of the obtained resins was examined against Escherichia coli and Staphylococcus aureus. Resins containing phenolic hydroxy groups of 2.3–7.7 mequiv/g were obtained. Antibacterial activity of the resins containing various phenol derivatives against E. coli or S. aureus increased in the order of RAAS-4G-GA > RAAS-4G-DHBA > RAAS-4G-pHBA. The resins containing phenol derivatives exhibited higher antibacterial activity against E. coli than against S. aureus and high activity even against bacteria in NaCl solution. Scanning electron micrographs showed that high antibacterial activity was brought about by the phenolic hydroxyl groups in the resin. © 1997 John Wiley & Sons, Inc. J Appl Polym Sci 66: 1621–1630, 1997 相似文献
27.
Masafumi Yamato Shuhei Obayashi Takashi Nishiyama Hideo Horibe Kohki Takahashi Kazuo Watanabe 《Polymer》2014
The effect of the high order structure of an isotactic polypropylene (PP) composite on the resistivity of composites containing magnetically aligned Ni particles was studied. Only a small amount of particles needed to be added for the composite material to become conducting after heating while in a magnetic field. The Ni columns formed on applying the field were distorted by the formation of large PP spherulites. Changes to the crystallization process due to the addition of a nucleating agent gave rise to changes in the columnar structure, resulting in large changes in the resistivity of the composite material. Controlling the high order structure of the polymer matrix including its morphology is very important in order to be able to control the magnetically aligned Ni structure. 相似文献
28.
Hiroki Eisawa Shun Ogawa Nobuhiro Yamazaki Kohki Maekawa Takahiro Yamaguchi Shota Sato Kazuma Shiota Takashi Yoshida 《Journal of Applied Glycoscience》2017,64(1):9
α-1,6-Glucosidase (isomaltase) belongs to glycoside hydrolase (GH) families 13 and 31. Genes encoding 3 isomaltases belonging to GH family 13 were cloned from filamentous fungi, Aspergillus oryzae (agl1), A. niger (agdC),and Fusarium oxysporum (foagl1), and expressed in Escherichia coli. The enzymes hydrolyzed isomaltose and α-glucosides preferentially at a neutral pH, but did not recognize maltose, trehalose, and dextran. The activity of AgdC and Agl1 was inhibited in the presence of 1 % glucose, while Foagl1 was more tolerant to glucose than the other two enzymes were. The three fungal isomaltases did not show transglycosylation when isomaltose was used as the substrate and a similar result was observed for AgdC and Agl1 when p-nitrophenyl-α-glucoside was used as the substrate. 相似文献
29.
We investigate the decomposition characteristics of phenol in an aqueous solution under exposure to pulsed discharge plasma. The investigation is carried out with different electrode configurations, applied voltages, and humidity levels and compositions of the background gas. It is possible that in the case of all gases, OH radicals are responsible for the decomposition of phenol in the solution. In pure O2, the decomposition rate of phenol increases due to the generation of O and O3. In pure N2, OH radicals produced by N2, which is excited in the metastable state, contribute to phenol decomposition. In N2‐O2, the decomposition rate of phenol remains low, and the NOx produced by the pulsed plasma in a N2‐O2 mixture destroys O3, and the production of the NOx inhibits O3 production. In Ar‐O2, the decomposition rate of phenol increases with an increase in the concentration of Ar in the mixture. It is possible that excited Ar atoms are responsible for the decomposition of phenol in the solution at higher concentrations of Ar in Ar‐O2. Further, it is found that the decomposition rate of phenol in aqueous solution exposed to a pulsed discharge is almost the same as that of phenol in aqueous solution exposed to a DC corona discharge. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 174(2): 1–8, 2011; Published online in Wiley Online Library ( wileyonlinelibrary.com ). DOI 10.1002/eej.20937 相似文献
30.
Naofumi Naga Gaku Ishikawa Keiichi Noguchi Kohki Takahashi Kazuo Watanabe Masafumi Yamato 《Polymer》2013,54(2):784-790
Magnetic-field induced alignment of polyethylene is firstly reported. Isothermal crystallization of low molecular weight polyethylene samples, high density polyethylene (HDPE) and linear low density polyethylene (LLDPE), from melting state was conducted under the magnetic-field of 2 or 10 T. Isothermal crystallization at the temperatures which induced effective self-seeding achieved alignment of polymer chains. The b axis of polyethylene unit cell was aligned parallel to the magnetic-field. Low viscosity and low crystallization rate of the polyethylene samples were favorable for high degree of the magnetic-field alignment. 相似文献