Characterization,Expression Profiling,and Biochemical Analyses of the Cinnamoyl-CoA Reductase Gene Family for Lignin Synthesis in Alfalfa Plants

Cinnamoyl-CoA reductase (CCR) is a pivotal enzyme in plant lignin synthesis, which has a role in plant secondary cell wall development and environmental stress defense. Alfalfa is a predominant legume forage with excellent quality, but the lignin content negatively affects fodder digestibility. Currently, there is limited information on CCR characteristics, gene expression, and its role in lignin metabolism in alfalfa. In this study, we identified 30 members in the CCR gene family of Medicago sativa. In addition, gene structure, conserved motif, and evolution analysis suggested MsCCR1–7 presumably functioned as CCR, while the 23 MsCCR-likes fell into three categories. The expression patterns of MsCCRs/MsCCR-likes suggested their role in plant development, response to environmental stresses, and phytohormone treatment. These results were consistent with the cis-elements in their promoters. Histochemical staining showed that lignin accumulation gradually deepened with the development, which was consistent with gene expression results. Furthermore, recombinant MsCCR1 and MsCCR-like1 were purified and the kinetic parameters were tested under four substrates. In addition, three-dimensional structure models of MsCCR1 and MsCCR-like1 proteins showed the difference in the substrate-binding motif H212(X)2K215R263. These results will be useful for further application for legume forage quality modification and biofuels industry engineering in the future.     

Extraction of salient textual patterns: synergy between lexical cohesion and contextual coherence

Most current information retrieval systems rely solely on lexical item repetition, which is notorious for its vulnerability. In this research, we propose a novel method for the extraction of salient textual patterns. One of our major objectives is to move away from keywords and their associated limitations in textual information retrieval. How individual sentences in text fit together to be perceived as a salient pattern is identified. A text network that exhibits textual continuity, arising from a connectionist model, is described. The network facilitates a dynamic extraction of salient textual segments by capturing semantics from two different categories of natural language, namely lexical cohesion and contextual coherence. We also present the results of an empirical study designed to compare our model with the performance of human judges in the identification of salient textual patterns. The preliminary results show that our model has the potential for automatic salient patterns discovery in text.     

消费电子技术发展对广播影视新媒体的影响

本文从终端显示技术、多媒体技术节能环保材料与技术等方面较全面的介绍了有关广电的消费类电子产品,最后提出了广播影视新媒体应对消费电子行业发展对策及建议.     

电力用户侧能源优化碳普惠体系设计及应用

碳普惠是一种引导广大公众践行节能减碳行为的新型激励机制。针对电力客户能源优化节碳潜力大但缺少常态化激励的问题,以碳普惠底层运行逻辑为指导开展电能用户能源优化碳普惠体系设计。首先面向碳减排方法学开发,从电力用户能源优化的减排机理分析出发,梳理了4类10种低碳行为,接着设计了政府监管下电网企业主导且多方参与下的碳普惠机制,并进行碳普惠服务平台技术架构设计;然后针对电动汽车能源替代场景构建了碳减排和碳积分计算模型;最后以江苏电动汽车碳普惠实践为例进行碳普惠应用效果分析,碳普惠实施后充电量增长2 123 kW·h,合计减碳量37 068 kg,站均碳积分激励系数为7.36%。算例表明碳普惠体系设计合理且对电动汽车移峰填谷起到较好的激励效果。     

Processing,Quality,Safety,and Acceptance of Meat Analogue Products

Rapid global population growth has caused an increasing need for products containing protein.Meat products are the most common high-protein food source,but impa...     

Factors Associated with White Fat Browning: New Regulators of Lipid Metabolism

Mammalian adipose tissue can be divided into white and brown adipose tissue based on its colour, location, and cellular structure. Certain conditions, such as sympathetic nerve excitement, can induce the white adipose adipocytes into a new type of adipocytes, known as beige adipocytes. The process, leading to the conversion of white adipocytes into beige adipocytes, is called white fat browning. The dynamic balance between white and beige adipocytes is closely related to the body’s metabolic homeostasis. Studying the signal transduction pathways of the white fat browning might provide novel ideas for the treatment of obesity and alleviation of obesity-related glucose and lipid metabolism disorders. This article aimed to provide an overview of recent advances in understanding white fat browning and the role of BAT in lipid metabolism.     

Cryogenic Exfoliation of 2D Stanene Nanosheets for Cancer Theranostics

Stanene(Sn)-based materials have been extensively applied in industrial production and daily life,but their potential biomedi-cal application remains largely un...     

多功能油层保护剂JQD的研制与应用

针对新疆小拐油田裂缝-孔隙型储层特点,开发研制了一种新型的兼具防漏堵漏及油层保护功能的JQD处理剂。该处理剂具有较好的油溶率、酸溶率,与现有钻井完井液体系配伍性良好,加工粒径可调,对1—4mm的裂缝堵漏效果良好。动态损害实验表明,JQD体系的岩心渗透率恢复值不小于80％。现场试验三口表明,JQD与钻井液配伍性良好,防漏堵漏效果显著,性能调整及维护方便,起下钻及电测顺利。三口井投产后,两口井产量分别为30和28t/d,一口井产气量为1.1×10~4m~3/d,说明储层得到了有效的保护,实现了“堵得住、解得开”的目标。