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41.
Cavitation and superstructure evolution of polymers during stretching play crucial roles to influence the mechanical properties of materials. In this study, we investigated deformation-mediated superstructures and cavitation of poly (l-lactide) (PLA) as well as their dependence on stretching temperatures by in-situ small-angle X-ray (SAXS) analysis coupled with mechanical testing. It is found that the cavitation and crystalline deformation are strongly influenced by stretching stress during deformation, which significantly depends on the stretching temperature. At lower stretching temperature (70 °C), the cavitation is initiated before the yielding and then stimulates the crystallite shearing. At higher stretching temperature (90 °C), however, the crystallites shear firstly and then crystalline deformation promotes the formation of cavities orientated along the stretching direction. High stretching temperature benefits the formation of relatively perfect crystals with high orientation. The results provide the basic knowledge of how to adjust the mechanical properties of polymer materials by controlling their superstructure in the deformation process.  相似文献   
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This paper reviews the methodology applied for the identification and characterisation of enterococci and covers phenotypic, genotypic and phylogenetic techniques. Although conventional phenotypic typing schemes are useful for rapid and simple identification of enterococcal species for routine applications, other methods like standardised sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE), multilocus enzyme electrophoresis (MLEE), antimicrobial susceptibility testing, serotyping, pyrolysis mass spectrometry (pyMS) and vibrational spectroscopic methods allow a more in-depth characterisation of enterococci. Many of the recently described enterococcal species exhibit deviations from hitherto so-called classical enterococci with regard to their phenotypical properties. Therefore, genotypic methods have to be used to clarify their possible assignment to the genus Enterococcus. In this review, special emphasis is given on recently developed polymerase chain reaction (PCR)-based typing methods such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), specific and random amplification (SARA) and modifications of PCR-ribotyping as well as pulsed-field gel electrophoresis (PFGE) and partial sequence analysis. The use of PCR and probes for genus and species identification of enterococci is also considered like the application of sequence data of conserved DNA regions (e.g., ribosomal ribonucleic acid (rRNA) genes) in the case of species identification.  相似文献   
43.
We address the issue of image sequence analysis jointly in space and time. While typical approaches to such an analysis consider two image frames at a time, we propose to perform this analysis jointly over multiple frames. We concentrate on spatiotemporal segmentation of image sequences and on analysis of occlusion effects therein. The segmentation process is three-dimensional (3-D); we search for a volume carved out by each moving object in the image sequence domain, or "object tunnel," a new space-time concept. We pose the problem in variational framework by using only motion information (no intensity edges). The resulting formulation can be viewed as volume competition, a 3-D generalization of region competition. We parameterize the unknown surface to be estimated, but rather than using an active-surface approach, we embed it into a higher dimensional function and apply the level-set methodology. We first develop simple models for the detection of moving objects over static background; no motion models are needed. Then, in order to improve segmentation accuracy, we incorporate motion models for objects and background. We further extend the method by including explicit models for occluded and newly exposed areas that lead to "occlusion volumes," another new space-time concept. Since, in this case, multiple volumes are sought, we apply a multiphase variant of the level-set method. We present various experimental results for synthetic and natural image sequences.  相似文献   
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Intrauterine growth restricted (IUGR) individuals have greater predisposition to develop a metabolic syndrome in later life manifesting itself as obesity, hypertension, type 2 diabetes or cardiovascular disease. Poor fetal growth may alter the regularity mechanism of cardiac autonomic system that is involved in the development of these diseases. The malfunctioning of the cardiac autonomic system assessed by decrease in heart rate variability (HRV) is associated with negative cardiovascular outcomes. This study is aimed at investigating the risk of development of coronary heart disease in IUGR children in later life. For that purpose HRV analysis of normal and growth restricted children was performed during sleep and wake stages. The study group consisted of 9–10 years old, 32 normal, 20 low birth weight IUGR children. The standard time domain HRV metrics (mean RR, SDNN, RMSSD, NN50 and pNN50) and Poincaré indices (SD1 and SD2) were used to analyse and compare the RR-interval time series of these groups. The IUGR children showed lower HRV as compared with normal children during both sleep and wake stages. The significantly decreased HRV during sleep provide an evidence of autonomic derangement that may be associated with higher risk of lethal arrhythmias in the IUGR children in later life.  相似文献   
46.
Traditional multivariate control charts such as Hotelling’s χ 2 and T 2 control charts are designed to monitor vectors of variable quality characteristics. However, in certain situations, data are expressed in linguistic terms and, under these circumstances, variable or attribute multivariate control charts are not suitable choices for monitoring purposes. Fuzzy multivariate control charts such as fuzzy Hotelling’s T 2 could be considered as efficient tools to overcome the problems of linguistic observations. The purpose of this paper is to develop a fuzzy multivariate exponentially weighted moving average (F-MEWMA) control chart. In this paper, multivariate statistical quality control and fuzzy set theory are combined to develop the proposed method. Fuzzy sets and fuzzy logic are powerful mathematical tools for modeling uncertain systems in industry, nature, and humanity. Through a numerical example, the performance of the proposed control chart was compared to the fuzzy Hotelling’s T 2 control chart. Results indicate uniformly superior performance of the F-MEWMA control chart over Hotelling’s T 2 control chart.  相似文献   
47.
Real-time automated risk assessment in protected core networking   总被引:1,自引:0,他引:1  
Protected Core Networking (PCN) is an approach to creating a secure and flexible network and communications infrastructure that supports network enabled capability (NEC) operations. The real-time automated risk assessment (R-TARA) provides a theoretically and practically sound method for risk assessment in the Protected Core. The purpose of the R-TARA is multifold. On the one hand it provides a global metric, which could be used by the network operator to assess the overall security level of the network and its evolution over time. On the other hand, the results of R-TARA can be used in order to achieve dynamic accreditation. Finally, R-TARA local risk metrics, e.g. susceptibility to DoS attacks, can be used for dynamic routing decisions. We propose use of Bayesian networks, known from operational risk assessment, for PCN risk assessment and we provide analytical and simulative evaluation of R-TARA mechanisms.  相似文献   
48.
In highly flexible and complex handling systems the risk of collision of moving machine components is continuously increasing. When, for example, several robots share the same workspace and the environment in the workspace is changing due to the operation, the robot trajectories can no longer be planned in advance without the risk of collision. The objective of this paper is the collision-free motion planning of handling system manipulators from the start to the end position within a changing environment. For this purpose, the trajectories on the control system have to be planned during the operating time most efficiently depending on the current state of a varying environment. This paper describes a new approach for trajectory planning based on curve shortening flows combined with potential fields. The functional capability of the method will be demonstrated initially on a simple robot kinematics with two degrees of freedom.  相似文献   
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