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71.
Matsui J Akamatsu K Nishiguchi S Miyoshi D Nawafune H Tamaki K Sugimoto N 《Analytical chemistry》2004,76(5):1310-1315
A molecularly imprinted polymer with immobilized Au nanoparticles (Au-MIP) is reported as a novel type of sensing material. The sensing mechanism is based upon the variable proximity of the Au nanoparticles immobilized in the imprinted polymer, which exhibits selective binding of a given analyte accompanied by swelling that causes a blue-shift in the plasmon absorption band of the immobilized Au nanoparticles. Using adrenaline as the model analyte, it was shown that molecular imprinting effectively enhanced the sensitivity and selectivity, and accordingly, Au-MIP selectively detects the analyte at 5 microM. The combination of molecular imprinting and the Au nanoparticle-based sensing system was shown to be a general strategy for constructing sensing materials in a tailor-made fashion due to wide applicability of the imprinting technique and the independence of the sensing mechanism from the analyte recognition system. 相似文献
72.
In this paper, we present a new learning method using prior information for three-layered neural networks. Usually when neural networks are used for identification of systems, all of their weights are trained independently, without considering their interrelation of weight values. Thus the training results are not usually good. The reason for this is that each parameter has its influence on others during the learning. To overcome this problem, first, we give an exact mathematical equation that describes the relation between weight values given by a set of data conveying prior information. Then we present a new learning method that trains a part of the weights and calculates the others by using these exact mathematical equations. In almost all cases, this method keeps prior information given by a mathematical structure exactly during the learning. In addition, a learning method using prior information expressed by inequality is also presented. In any case, the degree of freedom of networks (the number of 相似文献
73.
Germanium-oxide glass optical fibre doped with antimony for transmission of the 2 to 3 ?m band has been prepared by the vapour-phase axial deposition (VAD) method. An attenuation loss of 13 dB/km at 2.05 ?m and 70 dB/km at 2.4 ?m has been achieved. 相似文献
74.
The optical characteristics of a single-element hollow cube-corner retroreflector for a geosynchronous satellite were numerically evaluated for laser ranging and laser long-path absorption measurements of atmospheric species. An optical design with spherical surfaces and tuned dihedral angles was considered to compensate for velocity aberrations. The parameters for the retroreflector were optimized with genetic algorithms for different retroreflector sizes and wavelengths (500 nm and 1, 3, and 10 mum). We found that 20-cm retroreflectors are sufficient for realistic measurements when the laser wavelength is 500 nm or 1 mum. However, a larger retroreflector is necessary to overcome the detector noise level at 3 and 10 mum. 相似文献
75.
A. Idesaki M. Narisawa K. Okamura M. Sugimoto Y. Morita T. Seguchi M. Itoh 《Journal of Materials Science》2001,36(2):357-362
A fine SiC fiber is synthesized from a polymer blend of polycarbosilane (PCS) and polyvinylsilane (PVS) with electron beam curing under vacuum. The obtained SiC fiber from the PCS-PVS blend polymer has smaller average diameter of 8.5 m than that of 11.8 m from PCS, and shows higher average tensile strength of 3.2 GPa than that of 2.8 GPa from PCS after heat treatment at 1673 K in Ar gas atmosphere. However, the SiC fiber from the polymer blend decreases in tensile strength after heat treatment above 1773 K due to -SiC crystal growth near the fiber surface, because of a small amount of oxygen incorporated in the fiber. 相似文献
76.
S Maruta Y Uyehara K Homma Y Sugimoto K Wakabayashi 《Canadian Metallurgical Quarterly》1999,125(1):177-185
The products of the SOS-regulated umuDC operon are required for most UV and chemical mutagenesis in Escherichia coli, a process that results from a translesion synthesis mechanism. The UmuD protein is activated for its role in mutagenesis by a RecA-facilitated autodigestion that removes the N-terminal 24 amino acids. A previous genetic screen for nonmutable umuD mutants had resulted in the isolation of a set of missense mutants that produced UmuD proteins that were deficient in RecA-mediated cleavage (J. R. Battista, T. Ohta, T. Nohmi, W. Sun, and G. C. Walker, Proc. Natl. Acad. Sci. USA 87:7190-7194, 1990). To identify elements of the UmuD' protein necessary for its role in translesion synthesis, we began with umuD', a modified form of the umuD gene that directly encodes the UmuD' protein, and obtained missense umuD' mutants deficient in UV and methyl methanesulfonate mutagenesis. The D39G, L40R, and T51I mutations affect residues located at the UmuD'2 homodimer interface and interfere with homodimer formation in vivo. The D75A mutation affects a highly conserved residue located at one end of the central strand in a three-stranded beta-sheet and appears to interfere with UmuD'2 homodimer formation indirectly by affecting the structure of the UmuD' monomer. When expressed from a multicopy plasmid, the L40R umuD' mutant gene exhibited a dominant negative effect on a chromosomal umuD+ gene with respect to UV mutagenesis, suggesting that the mutation has an effect on UmuD' function that goes beyond its impairment of homodimer formation. The G129D mutation affects a highly conserved residue that lies at the end of the long C-terminal beta-strand and results in a mutant UmuD' protein that exhibits a strongly dominant negative effect on UV mutagenesis in a umuD+ strain. The A30V and E35K mutations alter residues in the N-terminal arms of the UmuD'2 homodimer, which are mobile in solution. 相似文献
77.
Ken’ichiro Kita Masaki Narisawa Atsushi Nakahira Hiroshi Mabuchi Masayoshi Itoh Masaki Sugimoto Masahito Yoshikawa 《Journal of Materials Science》2010,45(1):139-145
The polymer blends of PCS (polycarbosilane) and PMHS-h (polymethylohydrosiloxane with high molecular weight) were prepared
by freeze-drying process of mixed benzene solution. Melt viscosity, mass loss, and gas evolution from prepared polymer blends
were analyzed. A polymer blend of HSah15 (15 mass% PMHS-h to PCS) was melt-spun to fiber form, curing by thermal oxidation
and pyrolyzed at various temperatures up to 1773 K. The obtained fibers were investigated by tensile tests, FE-SEM (field
emission scanning electron microscope) observation, and XRD (X-ray diffraction) analysis. After pyrolysis at 1273 K, there
were no pores in the cross section of the fiber derived from pure PCS; however, there were amounts of pores in the cross sections
of the fiber derived from HSah15. After pyrolysis at 1773 K, the coarse β-SiC (silicon carbide) crystals were formed on the
outside surface of the fiber derived from pure PCS; however, no remarkable β-SiC crystal were formed on the outside surface
of the fiber derived from HSah15. 相似文献
78.
Hongo Takero Arakawa Hideo Sugimoto Gunji Tange Koichi Yamamoto Yuzo 《Industrial Electronics, IEEE Transactions on》1987,(1):5-10
An automatically guided vehicle, traveling without fixed guide ways, has been developed. In this paper, the construction of the vehicle, the control algorithm, and its general performance are described. 相似文献
79.
Shigeki Tasaka Yoshihiro Sato Nobuhito Tateyama Hisahiko Sugimoto Fumihito Fumuro Kaoru Hoshino 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》1985,236(2):400-409
A new multiple scanning method is developed and applied to detect cosmic heavy nuclei. The plastic track detectors CR-39 exposed to cosmic heavy nuclei are over-etched in 8.0N NaOH for 94 h at 80°C. We found that the etched cones can easily be scanned on the plastic plates without using an optical microscope. The trajectories of heavy nuclei can be reconstructed with the naked eyes by superposing four or more over-etched plates. The charge detection threshold is obtained by this method, and appears to be 10. The charge resolution is estimated to be 1.0 charge units for iron nuclei. By adopting this method, the scanning time is much shortened and CR-39 plastic chamber size can easily be scaled up. The method is very useful for detecting particles with low flux intensity, like for searching magnetic monopoles or for measuring the high energy spectra of cosmic heavy nuclei. 相似文献
80.