High-resolution electron microscopy of the twinning and intergrowth in Ca4Al6SO16 and Ca3SrAl6SO16

The microstructures of unhydrated calcium aluminosulphate Ca₄Al₆SO₁₆ and Ca₃SrAl₆SO₁₆ have been studied by high-resolution electron microscopy (HREM). The results showed that twinning and twinned slabs could be introduced taking the [1 1 2] direction as the twin axis so that it seems to be coincident with the law of twinning formed in body-centred cubic structures. A previously reported superlattice with a repeat period twice that of the fundamental structure along the 〈1 1 0〉 direction has also been found in both matrix and twin variants. The close intergrowth of Ca₃SrAl₆SO₁₆ and another phase, possibly Sr₃Al₂O₆ existing as an inclusion between these two twin variants, was determined and clearly revealed by electron diffraction and HREM images. The coherent interphase boundaries and orientation relationship between them can also be deduced.     

A model for preventive maintenance operations and forecasting

Equipment costs constitute the greatest majority of overall costs for semiconductor manufacturing. Therefore, maintaining high equipment availability has been regarded as one of the major goals in the industry. The ability to forecast correctly equipment preventive maintenance (PM) timing requirements not only can help optimizing equipment uptime but also minimizing negative impacts on manufacturing production efficiency. This research used grey theory and evaluation diagnosis to construct a PM forecasting model for prediction of PM timing of various machines. The results showed significant improvements of PM timing predictions compared to the existing method based on experience and an alternative method proposed by Li and Chang (Semiconductor Manufacturing Technology Workshop 2002: 10–11, pp. 275–277) for the same fab cases. Received: June 2005 / Accepted: December 2005     

Response of Vietnam coastal upwelling to the 1997-1998 ENSO event observed by multisensor data

In this paper, we examine the behavior of the Vietnam coastal upwelling during the 1997-1998 El Niño-Southern Oscillation (ENSO) event. The baseline is 4 years of National Oceanic and Atmospheric Administration (NOAA) satellite Advanced Very High Resolution Radiometer (AVHRR) sea surface temperature (SST) data taken from 1997 to 2000. Comparison of upwelling images to simultaneous ERS-2 (European Remote Sensing Satellite) wind fields indicates that the summer monsoon winds constitute a major generation forcing. During the 1997 El Niño, the monsoon winds enhanced the upwelling and induced the upwelling center to move southward. During the 1998 La Niña, the monsoon winds weakened the upwelling. In contrast with the tropical Pacific, in the study area, La Niña implies a warm event and El Niño a cold event. We use empirical orthogonal function (EOF) methods to analyze the spatial and temporal variance of the upwelling. The three principal modes account for 37%, 15%, and 8% of the total variance, respectively. The first EOF modes reveal that the SST variance in the north and south subregions underwent a positive-negative sign switch in summer 1997. The second EOF modes represent the monthly evolution in normal years. The third modes seem to be sensitive to the 1998 La Niña event. Simultaneous TOPEX/POSEIDON and ERS-2 altimeter data provide further evidence for our analysis. Comparison with California coastal upwelling and mid-Atlantic Bight (MAB) coastal upwelling indicates that the Vietnam coastal upwelling is the most intensive one.     

Mutational analysis of the three cysteines and active-site aspartic acid 103 of ketosteroid isomerase from Pseudomonas putida biotype B

In order to clarify the roles of three cysteines in ketosteroid isomerase (KSI) from Pseudomonas putida biotype B, each of the cysteine residues has been changed to a serine residue (C69S, C81S, and C97S) by site-directed mutagenesis. All cysteine mutations caused only a slight decrease in the k(cat) value, with no significant change of Km for the substrate. Even modification of the sulfhydryl group with 5,5'-dithiobis(2-nitrobenzoic acid) has almost no effect on enzyme activity. These results demonstrate that none of the cysteines in the KSI from P. putida is critical for catalytic activity, contrary to the previous identification of a cysteine in an active-site-directed photoinactivation study of KSI. Based on the three-dimensional structures of KSIs with and without dienolate intermediate analog equilenin, as determined by X-ray crystallography at high resolution, Asp-103 was found to be located within the range of the hydrogen bond to the equilenin. To assess the role of Asp-103 in catalysis, Asp-103 has been replaced with either asparagine (D103N) or alanine (D103A) by site-directed mutagenesis. For D103A mutant KSI there was a significant decrease in the k(cat) value: the k(cat) of the mutant was 85-fold lower than that of the wild-type enzyme; however, for the D103N mutant, which retained some hydrogen bonding capability, there was a minor decrease in the k(cat) value. These findings support the idea that aspartic acid 103 in the active site is an essential catalytic residue involved in catalysis by hydrogen bonding to the dienolate intermediate.     

A 1.5-V full-swing bootstrapped CMOS large capacitive-load drivercircuit suitable for low-voltage CMOS VLSI

This paper reports a 1.5-V full-swing bootstrapped CMOS large capacitive-load driver circuit using two bootstrap capacitors to enhance the switching speed for low-voltage CMOS VLSI. For a supply voltage of 1.5 V, the full-swing bootstrapped CMOS driver circuit shows a 2.2 times improvement in switching speed in driving a capacitive load of 10 pF as compared to the conventional CMOS driver circuit. Even for a supply voltage of 1 V, this full-swing bootstrapped CMOS large capacitive-load driver circuit is still advantageous     

A BiCMOS dynamic carry lookahead adder circuit for VLSIimplementation of high-speed arithmetic unit

A BiCMOS dynamic carry lookahead circuit that is free from race problems is presented. A 16 b full-adder test circuit, which has been designed based on a 2 μm BiCMOS technology, shows a more than five times improvement in speed as compared to the CMOS Manchester carry lookahead (MCLA) circuit. The speed advantage of the BiCMOS dynamic carry lookahead circuit is even greater in a 32- or 64-b adder     

Intercellular communication between dorsal root ganglion cells and colonic smooth muscle cells in vitro

This study demonstrates that the use of high field 1H NMR spectroscopy permits individual detection of phosphatidylcholine and sphingomyelin molecules at the surface of native low density lipoprotein (LDL) particles. Distinct behaviour was observed for the choline head group -N(CH3)3 resonances of these different phospholipids revealing preferential immobilisation for phosphatidylcholine. This suggests the existence of reversible and irreversible phosphatidylcholine-apolipoprotein B interactions and is consistent with microdomain formation at the surface monolayer of LDL. The novel resonance assignment and results show that 1H NMR can provide efficient and practical means for future studies on the structure and dynamics at the LDL surface.