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61.
Regio‐ and Enantioselective Sequential Dehalogenation of rac‐1,3‐Dibromobutane by Haloalkane Dehalogenase LinB 下载免费PDF全文
Johannes Gross Prof. Zbyněk Prokop Prof. Dick Janssen Prof. Kurt Faber Dr. Mélanie Hall 《Chembiochem : a European journal of chemical biology》2016,17(15):1437-1441
The hydrolytic dehalogenation of rac‐1,3‐dibromobutane catalyzed by the haloalkane dehalogenase LinB from Sphingobium japonicum UT26 proceeds in a sequential fashion: initial formation of intermediate haloalcohols followed by a second hydrolytic step to produce the final diol. Detailed investigation of the course of the reaction revealed favored nucleophilic displacement of the sec‐halogen in the first hydrolytic event with pronounced R enantioselectivity. The second hydrolysis step proceeded with a regioselectivity switch at the primary position, with preference for the S enantiomer. Because of complex competition between all eight possible reactions, intermediate haloalcohols formed with moderate to good ee ((S)‐4‐bromobutan‐2‐ol: up to 87 %). Similarly, (S)‐butane‐1,3‐diol was formed at a maximum ee of 35 % before full hydrolysis furnished the racemic diol product. 相似文献
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The entertainment industry, primarily the video games industry, continues to dictate the development and performance requirements
of graphics hardware and computer graphics algorithms. However, despite the enormous progress in the last few years, it is
still not possible to achieve some of industry’s demands, in particular high-fidelity rendering of complex scenes in real-time,
on a single desktop machine. A realisation that sound/music and other senses are important to entertainment led to an investigation
of alternative methods, such as cross-modal interaction in order to try and achieve the goal of “realism in real-time”. In
this paper we investigate the cross-modal interaction between vision and audition for reducing the amount of computation required
to compute visuals by introducing movement related sound effects. Additionally, we look at the effect of camera movement speed
on temporal visual perception. Our results indicate that slow animations are perceived as smoother than fast animations. Furthermore,
introducing the sound effect of footsteps to walking animations further increased the animation smoothness perception. This
has the consequence that for certain conditions, the number of frames that need to be rendered each second can be reduced,
saving valuable computation time, without the viewer being aware of this reduction. The results presented are another step
towards the full understanding of the auditory-visual cross-modal interaction and its importance for helping achieve “realism
int real-time”. 相似文献
66.
Interface structure of diffusion bonded duplex stainless steel and medium carbon steel couple 总被引:2,自引:0,他引:2
Diffusion bonding of duplex stainless steel to medium carbon steel was carried out with different temperatures for sound bonds. In the bonding process, relatively intermediate temperatures such as 750, 800, 850 and 900 °C were used with a bonding time of 30 min. In this study, microstructural changes and mechanical properties in the interface region of duplex stainless steel and medium carbon steel couples were determined. The results showed that, in interface region, Cr23C6 was formed on the stainless steel side, while ferrite formation was observed on the carbon steel side as a result of mutual diffusion of C and Cr. 相似文献
67.
Gawronski Bertram; LeBel Etienne P.; Peters Kurt R.; Banse Rainer 《Canadian Metallurgical Quarterly》2009,135(3):369
J. De Houwer, S. Teige-Mocigemba, A. Spruyt, and A. Moors’s (see record 2009-05290-001) normative analysis of implicit measures provides an excellent clarification of several conceptual ambiguities surrounding the validation and use of implicit measures. The current comment discusses an important, yet unacknowledged, implication of J. De Houwer et al.’s analysis, namely, that investigations addressing the proposed implicitness criterion (i.e., does the relevant psychological attribute influence measurement outcomes in an automatic fashion?) will be susceptible to fundamental misinterpretations if they are conducted independently of the proposed what criterion (i.e., is the measurement outcome causally produced by the psychological attribute the measurement procedure was designed to assess?). As a solution, it is proposed that experimental validation studies should be combined with a correlational approach in order to determine whether a given manipulation influenced measurement scores via variations in the relevant psychological attribute or via secondary sources of systematic variance. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
68.
Robert L. Wolff Olivier Lavialle Frédérique Pédrono Elodie Pasquier Laurent G. Deluc Anne M. Marpeau Kurt Aitzetmüller 《Lipids》2001,36(5):439-451
Following our previous review on Pinus spp. seed fatty acid (FA) compositions, we recapitulate here the seed FA compositions of Larix (larch), Picea (spruce), and Pseudotsuga (Douglas fir) spp. Numerous seed FA compositions not described earlier are included. Approximately 40% of all Picea taxa and one-third of Larix taxa have been analyzed so far for their seed FA compositions. Qualitatively, the seed FA compositions in the three genera
studied here are the same as in Pinus spp., including in particular the same Δ5-olefinic acids. However, they display a considerably lower variability in Larix and Picea spp. than in Pinus spp. An assessment of geographical variations in the seed FA composition of P. abies was made, and intraspecific dissimilarities in this species were found to be of considerably smaller amplitude than interspecific
dissimilarities among other Picea species. This observation supports the use of seed FA compositions as chemotaxonomic markers, as they practically do not
depend on edaphic or climatic conditions. This also shows that Picea spp. are coherently united as a group by their seed FA compositions. This also holds for Larix spp. Despite a close resemblance between Picea and Larix spp. seed FA compositions, principal component analysis indicates that the minor differences in seed FA compositions between
the two genera are sufficient to allow a clear-cut individualization of the two genera. In both cases, the main FA is linoleic
acid (slightly less than one-half of total FA), followed by pinolenic (5,9,12-18:3) and oleic acids. A maximum of 34% of total
Δ5-olefinic acids is reached in L. sibirica seeds, which appears to be the highest value found in Pinaceae seed FA. This apparent limit is discussed in terms of regio-
and stereospecific distribution of Δ5-olefinic acids in seed triacylglycerols. Regarding the single species of Pseudotsuga analyzed so far (P. menziesii), its seed FA composition is quite distinct from that of the other two genera, and in particular, it contains 1.2% of 14-methylhexadecanoic
(anteiso-17:0) acid. In the three genera studied here, as well as in most Pinus spp., the C18Δ5-olefinic acids (5,9-18:2 and 5,9,12-18:3 acids) are present in considerably higher amounts than the C20Δ5-olefinic acids (5,11-20:2 and 5,11,14-20:3 acids). 相似文献
69.
Efficient real-time trajectory tracking 总被引:1,自引:0,他引:1
Ralph Lange Frank Dürr Kurt Rothermel 《The VLDB Journal The International Journal on Very Large Data Bases》2011,20(5):671-694
Moving objects databases (MOD) manage trajectory information of vehicles, animals, and other mobile objects. A crucial problem
is how to efficiently track an object’s trajectory in real-time, in particular if the trajectory data is sensed at the mobile
object and thus has to be communicated over a wireless network. We propose a family of tracking protocols that allow trading
the communication cost and the amount of trajectory data stored at a MOD off against the spatial accuracy. With each of these
protocols, the MOD manages a simplified trajectory that does not deviate by more than a certain accuracy bound from the actual
movement. Moreover, the different protocols enable several trade-offs between computational costs, communication cost, and
the reduction in the trajectory data: Connection-Preserving Dead Reckoning minimizes the communication cost using dead reckoning, a technique originally designed for tracking an object’s current position.
Generic Remote Trajectory Simplification (GRTS) further separates between tracking of the current position and simplification of the past trajectory and can be realized
with different line simplification algorithms. For both protocols, we discuss how to bound the space consumption and computing
time at the moving object and thereby present an effective compression technique to optimize the reduction performance of
real-time line simplification in general. Our evaluations with hundreds of real GPS traces show that a realization of GRTS
with a simple simplification heuristic reaches 85–90% of the best possible reduction rate, given by retrospective offline
simplification. A realization with the optimal line simplification algorithm by Imai and Iri even reaches more than 97% of
the best possible reduction rate. 相似文献
70.
Improving classroom learning by collaboratively observing human tutoring videos while problem solving. 总被引:1,自引:0,他引:1
Craig Scotty D.; Chi Michelene T. H.; VanLehn Kurt 《Canadian Metallurgical Quarterly》2009,101(4):779
Collaboratively observing tutoring is a promising method for observational learning (also referred to as vicarious learning). This method was tested in the Pittsburgh Science of Learning Center’s Physics LearnLab, where students were introduced to physics topics by observing videos while problem solving in Andes, a physics tutoring system. Students were randomly assigned to three groups: (a) pairs collaboratively observing videos of an expert human tutoring session, (b) pairs observing videos of expert problem solving, or (c) individuals observing expert problem solving. Immediate learning measures did not display group differences; however, long-term retention and transfer measures showed consistent differences favoring collaboratively observing tutoring. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献