Diversity of lactic acid bacteria from Hussuwa, a traditional African fermented sorghum food

The diversity of lactic acid bacteria associated with Hussuwa fermentation, a Sudanese fermented sorghum food, was studied using a polyphasic taxonomical approach. Predominant strains could be well characterised based on a combination of phenotypic tests and genotypic methods such as ARDRA, rep-PCR and RAPD-PCR, as well as 16S rRNA gene sequencing of representative strains. Thus, the majority (128 of 220, 58.3%) of strains exhibited phenotypic properties typical of heterofermentative lactobacilli and of these, 100 strains were characterised more closely using the genotyping methods. The majority (97/100) strains could be characterised as Lactobacillus fermentum strains. Seventy-two of 220 strains (32.7%) showed phenotypic properties that are characteristic of pediococci. Of 41 selected strains investigated by genotyping techniques, 38 (92.7%) could be characterised as Pediococcus acidilactici strains, while three (7.3%) could be characterised as Pediococcus pentosaceus strains. The Hussuwa fermentation thus appears to be dominated by L. fermentum strains and P. acidilactici strains. For this reason, we selected representative and predominant strains as potential starter cultures for Hussuwa fermentation. These strains, L. fermentum strains BFE 2442 and BFE 2282 and P. acidilactici strain BFE 2300, were shown on the basis of RAPD-PCR fingerprinting to predominate in a model fermentation when used as starter cultures inoculated at 1 × 10⁶ CFU/g and to lower the pH of the fermentation to below pH 4.0 within 48 h. These cultures should be studied for further development as starter preparations in pilot scale studies in actual field fermentations.     

An Endosymbiotic Evolutionary Algorithm for Optimization

This paper proposes a new symbiotic evolutionary algorithm to solve complex optimization problems. This algorithm imitates the natural evolution process of endosymbionts, which is called endosymbiotic evolutionary algorithm. Existing symbiotic algorithms take the strategy that the evolution of symbionts is separated from the host. In the natural world, prokaryotic cells that are originally independent organisms are combined into an eukaryotic cell. The basic idea of the proposed algorithm is the incorporation of the evolution of the eukaryotic cells into the existing symbiotic algorithms. In the proposed algorithm, the formation and evolution of the endosymbionts is based on fitness, as it can increase the adaptability of the individuals and the search efficiency. In addition, a localized coevolutionary strategy is employed to maintain the population diversity. Experimental results demonstrate that the proposed algorithm is a promising approach to solving complex problems that are composed of multiple sub- problems interrelated with each other.     

Cloning and functional characterization of a Caenorhabditis elegans muscarinic acetylcholine receptor

A cDNA clone encoding a muscarinic acetylcholine receptor (mAChR) has been isolated from the nematode Caenorhabditis elegans. The nematode mAChR, consisted of 585 amino acids, displays a high degree of amino acid sequence homology to other invertebrate and vertebrate mAChRs. Excluding a highly variable middle portion of the third intracellular loop, the C. elegans mAChR shares about 51% amino acid sequence identity with a Drosophila mAChR and 42-44% identity with human m1-m5 mAChR subtypes. Comparison of the cDNA sequence with the corresponding genomic sequence reveals that the C. elegans mAChR gene contains ten introns, eight of them in the coding region. Pharmacological profiles of the C. elegans mAChR expressed in Chinese hamster ovary (CHO) cells were shown to be similar to those of mammalian counterparts, indicating that ligand binding domains of the receptor have been conserved during evolution. When this cloned receptor was expressed in Xenopus oocytes, acetylcholine evoked a transient Cl- current. Furthermore, activation of the receptor with oxotremorine, acetylcholine or carbachol resulted in the stimulation of phosphatidylinositol metabolism in CHO cells, suggesting that the receptor is coupled to phospholipase C activation.     

Synthesis of Al2O3-WC composite powder by SHS process

Al₂O₃-WC composite powder was synthesized by self-propagating high-temperature synthesis using Al powder as a reducing agent. WC, W₂C and Al₂O₃ were concurrently formed in WO₃-Al-C system. It was found that the complete reaction was achieved with excessive addition of carbon and appropriate processing parameters such as degree of dilution, particle size of aluminum, pellet compaction pressure and carbon source. The final product which was leached by 50% 1 : 4 HNO₃ + HF diluted solution was consisted of Al₂O₃-55wt%WC having 2–3 m of mean particle size.     

Polarity correspondence: A general principle for performance of speeded binary classification tasks.

Differences in performance with various stimulus-response mappings are among the most prevalent findings for binary choice reaction tasks. The authors show that perceptual or conceptual similarity is not necessary to obtain mapping effects; a type of structural similarity is sufficient. Specifically, stimulus and response alternatives are coded as positive and negative polarity along several dimensions, and polarity correspondence is sufficient to produce mapping effects. The authors make the case for this polarity correspondence principle using the literature on word-picture verification and then provide evidence that polarity correspondence is a determinant of mapping effects in orthogonal stimulus-response compatibility, numerical judgment, and implicit association tasks. The authors conclude by discussing implications of this principle for interpretation of results from binary choice tasks and future model development. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Real Time Traffic Information Service Using Terrestrial Digital Multimedia Broadcasting System

The major contribution of this paper is the implementation of real time traffic and traveler information service through the terrestrial digital multimedia broadcasting (T-DMB) system, which are known to be suitable for the mobile data services as well as audio and video services. The implemented system converts the Korean characteristic traffic information into the transport protocol expert group (TPEG) messages, and provides them to the T-DMB system. By testing the service with the TPEG message decoder implemented in the navigation system on PDA and laptop, it has turned out that it can be used as a highly efficient traffic information service system that could be adopted in Korea as well as in European countries     

A CMOS transistor-only 8-b 4.5-Ms/s pipelined analog-to-digitalconverter using fully-differential current-mode circuit techniques

Fully-differential current-mode circuit techniques are developed for the design of a pipelined current-mode analog-to-digital converter (IADC) in the standard CMOS digital processes. In the proposed IADC, the 1-b-per-stage architecture based on the reference nonrestoring algorithm is adopted. Thus large component ratios can be avoided and the linearity errors caused by device mismatches can be minimized. As one of the key subcircuits in the IADC, an offset-canceled high speed differential current comparator (CCMP) is proposed and analyzed. In the CCMP, the subtractions of offsets are performed in the current domain without floating capacitors. Moreover, the other key subcircuit, the current sample-and-hold amplifier (CSHA), is also developed to realize the pipeline architecture. An experimental chip for the proposed IADC has been fabricated in 0.8-μm n-well CMOS technology. Using a single 5-V power supply, the fabricated IADC can be operated at 4.5-Ms/s conversion rate with a signal-to-noise-and-distortion-ratio (SNDR) of 51 db (effective 8.2-b) for the input signal at 453 kHz. For 8-b resolution, the fabricated IADC can be operated at 4.5-Ms/s conversion rate with both differential nonlinearity (DNL) and integral nonlinearity (INL) below +/-0.6 LSB. The power consumption and the active chip area are 16 mW/b and 0.73 mm²/b, respectively     

Changes in brain-derived neurotrophic factor immunoreactivity in rat dorsal root ganglia, spinal cord, and gracile nuclei following cut or crush injuries

In the present study, we evaluated changes in brain-derived neurotrophic factor (BDNF) immunoreactivity in the rat lumbar (L) 5 dorsal root ganglion (DRG) and areas where afferents from the DRG terminate, the L5 spinal cord and gracile nuclei, following unilateral sciatic nerve transection or crush. From 3 days to 4 weeks following cut or crush injury, the percentage of medium and large BDNF-immunoreactive neurons in the ipsilateral DRG increased significantly compared with those on the contralateral side. Following cut injury, there was no significant change in the percentage of small BDNF-immunoreactive neurons in the ipsilateral DRG; however, the intensity of immunoreactivity of these cells decreased. Following crush injury, however, both the percentage and intensity of small BDNF-immunoreactive neurons in the ipsilateral DRG significantly increased. Following cut injury, the expression of BDNF-immunoreactive axonal fibers decreased markedly in the ipsilateral superficial laminae of the L5 spinal cord and increased significantly in the ipsilateral deeper laminae of the spinal cord and gracile nuclei. Crush injury induced a marked increase in the expression of BDNF-immunoreactive axonal fibers in the superficial laminae of the spinal cord and gracile nuclei. These differences in BDNF response in the DRG and spinal cord after cut or crush injuries may reflect differences in trophic support to the injured DRG neurons and altered neuronal activity in the spinal cord and gracile nuclei following different types of peripheral nerve injury.     

Octree-R: an adaptive octree for efficient ray tracing

Ray tracing requires many ray-object intersection tests. A way of reducing the number of ray-object intersection tests is to subdivide the space occupied by objects into many nonoverlapping subregions, called voxels, and to construct an octree for the subdivided space. We propose the Octree-R, an octree-variant data structure for efficient ray tracing. The algorithm for constructing the Octree-R first estimates the number of ray-object intersection tests. Then, it partitions the space along the plane that minimizes the estimated number of ray-object intersection tests. We present the results of experiments for verifying the effectiveness of the Octree-R. In the experiment, the Octree-R provides a 4% to 47% performance gain over the conventional octree. The result shows the more skewed the object distribution (as is typical for real data), the more performance gain the Octree-R achieves