Vibrio parahaemolyticus is an important food-borne enteropathogen that encounters various adverse conditions in its native environment or during infection. Effects of mild acid treatment on survival under stress conditions, enteropathogenicity, and protein production in this pathogen were investigated. Logarithmically grown cells, at pH 7.5 shifted to pH 5.0 for 30 min, were more resistant to subsequent acid challenge at pH 4.4. A two-phase adaptive procedure (pH 5.8 for 30 min; pH 5.0 for 30 min) was better than a single-phase procedure for enhancing the acid tolerance of this pathogen. The acid-adapted cells were cross-protected against the challenges of low salinity and thermal inactivation. One-dimensional polyacrylamide gel electrophoresis revealed that proteins with molecular masses of 6.4, 9.0, 13.6, 16.3, 18.9, 22.9, 24.4, 28.3, 33. 9, 36.9, 41.2, 47.6, 58.1, 65.6, 80.5, 88.2, and 96.9 kDa were induced or significantly enhanced, while proteins of 25.3, 30.1, 30. 7, and 91.7 kDa were significantly inhibited. Two-dimensional polyacrylamide gel electrophoresis revealed that 20 species of proteins were induced or significantly enhanced, while 26 species were inhibited. In assays conducted using the suckling mouse model, enteropathogenicity of the acid-adapted cells was significantly enhanced in terms of intestine/body weight ratio and in vivo recovery of infected cells. 相似文献
The three-dimensional wedge-shaped underwater acoustic propagation model exists analytical solution, which provides verification for models like FOR3D propagation model under certain situation. However, the solving process of a three-dimensional complex underwater sound field problem is hindered by intensive computing and long calculation times. In this paper, we exploit a hybrid parallel programing model, such as MPI and OpenMP, to accelerate the computation, design various optimization methods to improve the overall performance, and then carry out the performance and optimization analysis on the Tianhe-2 platform. Experiments show that the optimized implementation of the three-dimensional wedge-shaped underwater acoustic propagation model achieves a 46.5 speedup compared to the original serial program, thereby illustrating a substantial performance improvement. We also carried out scalability tests and parallel optimization experiments for large-scale practical examples.
The present work proposes a solution to the challenging problem of registering two partial point sets of the same object with very limited overlap. We leverage the fact that most objects found in man-made environments contain a plane of symmetry. By reflecting the points of each set with respect to the plane of symmetry, we can largely increase the overlap between the sets and therefore boost the registration process. However, prior knowledge about the plane of symmetry is generally unavailable or at least very hard to find, especially with limited partial views. Finding this plane could strongly benefit from a prior alignment of the partial point sets. We solve this chicken-and-egg problem by jointly optimizing the relative pose and symmetry plane parameters. We present a globally optimal solver by employing the branch-and-bound paradigm and thereby demonstrate that joint symmetry plane fitting leads to a great improvement over the current state of the art in globally optimal point set registration for common objects. We conclude with an interesting application of our method to dense 3D reconstruction of scenes with repetitive objects.
This paper proposes a framework for business process design, which is based on process comparison and integration. This framework
supports process stakeholders to model business processes collaboratively, which can greatly enhance the efficiency and effectiveness
of business process design. By applying this framework, each process stakeholder can create his or her own model of the business
process. The different models of the same business process can be automatically compared to find the conflicts between them.
Based on the conflicts, process stakeholders can collaborate to improve the models. Then the models can be integrated to form
a more complete model. 相似文献