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41.
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Svetlana V. Klinova Ilzira A. Minigalieva Yuri L. Protsenko Marina P. Sutunkova Vladimir B. Gurvich Julia V. Ryabova Irene E. Valamina Oksana P. Gerzen Salavat R. Nabiev Alexander A. Balakin Oleg N. Lookin Ruslan V. Lisin Daniil A. Kuznetsov Larisa I. Privalova Vladimir G. Panov Leonid B. Katsnelson Larisa V. Nikitina Boris A. Katsnelson 《International journal of molecular sciences》2022,23(8)
Exposure to lead is associated with an increased risk of cardiovascular diseases. Outbred white male rats were injected with lead acetate intraperitoneally three times a week and/or were forced to run at a speed of 25 m/min for 10 min 5 days a week. We performed noninvasive recording of arterial pressure, electrocardiogram and breathing parameters, and assessed some biochemical characteristics. Electrophoresis in polyacrylamide gel was used to determine the ratio of myosin heavy chains. An in vitro motility assay was employed to measure the sliding velocity of regulated thin filaments on myosin. Isolated multicellular preparations of the right ventricle myocardium were used to study contractility in isometric and physiological modes of contraction. Exercise under lead intoxication normalized the level of calcium and activity of the angiotensin-converting enzyme in the blood serum, normalized the isoelectric line voltage and T-wave amplitude on the electrocardiogram, increased the level of creatine kinase-MB and reduced the inspiratory rate. Additionally, the maximum sliding velocity and the myosin heavy chain ratio were partly normalized. The effect of exercise under lead intoxication on myocardial contractility was found to be variable. In toto, muscular loading was found to attenuate the effects of lead intoxication, as judged by the indicators of the cardiovascular system. 相似文献
43.
Vladimir V. Britikov Eduard V. Bocharov Elena V. Britikova Natalia I. Dergousova Olga G. Kulikova Anastasia Y. Solovieva Nikolai S. Shipkov Larisa A. Varfolomeeva Tamara V. Tikhonova Vladimir I. Timofeev Eleonora V. Shtykova Dmitry A. Altukhov Sergey A. Usanov Alexander S. Arseniev Tatiana V. Rakitina Vladimir O. Popov 《International journal of molecular sciences》2022,23(17)
The search of a putative physiological electron acceptor for thiocyanate dehydrogenase (TcDH) newly discovered in the thiocyanate-oxidizing bacteria Thioalkalivibrio paradoxus revealed an unusually large, single-heme cytochrome c (CytC552), which was co-purified with TcDH from the periplasm. Recombinant CytC552, produced in Escherichia coli as a mature protein without a signal peptide, has spectral properties similar to the endogenous protein and serves as an in vitro electron acceptor in the TcDH-catalyzed reaction. The CytC552 structure determined by NMR spectroscopy reveals significant differences compared to those of the typical class I bacterial cytochromes c: a high solvent accessible surface area for the heme group and so-called “intrinsically disordered” nature of the histidine-rich N- and C-terminal regions. Comparison of the signal splitting in the heteronuclear NMR spectra of oxidized, reduced, and TcDH-bound CytC552 reveals the heme axial methionine fluxionality. The TcDH binding site on the CytC552 surface was mapped using NMR chemical shift perturbations. Putative TcDH-CytC552 complexes were reconstructed by the information-driven docking approach and used for the analysis of effective electron transfer pathways. The best pathway includes the electron hopping through His528 and Tyr164 of TcDH, and His83 of CytC552 to the heme group in accordance with pH-dependence of TcDH activity with CytC552. 相似文献
44.
Lena Neuper Daniel Kummer Dsire Forstner Jacqueline Guettler Nassim Ghaffari-Tabrizi-Wizsy Cornelius Fischer Herbert Juch Olivia Nonn Martin Gauster 《International journal of molecular sciences》2022,23(20)
Angiotensin II receptor 1 blockers are commonly used to treat hypertension in women of childbearing age. While the fetotoxic effects of these drugs in the second and third trimesters of pregnancy are well documented, their possible impacts on placenta development in early gestation are unknown. Candesartan, a member of this group, also acts as a peroxisome proliferator-activated receptor gamma (PPARγ) agonist, a key regulator shown to be important for placental development. We have previously shown that trophoblasts do not express the candesartan target–receptor angiotensin II type 1 receptor AGTR1. This study investigated the possible role of candesartan on trophoblastic PPARγ and its hallmark target genes in early gestation. Candesartan did not affect the PPARγ protein expression or nuclear translocation of PPARγ. To mimic extravillous trophoblasts (EVTs) and cytotrophoblast/syncytiotrophoblast (CTB/SCT) responses to candesartan, we used trophoblast cell models BeWo (for CTB/SCT) and SGHPL-4 (EVT) cells as well as placental explants. In vitro, the RT-qPCR analysis showed no effect of candesartan treatment on PPARγ target genes in BeWo or SGHPL-4 cells. Treatment with positive control rosiglitazone, another PPARγ agonist, led to decreased expressions of LEP and PPARG1 in BeWo cells and an increased expression of PPARG1 in SGHPL-4 cells. Our previous data showed early gestation–placental AGTR1 expression in fetal myofibroblasts only. In a CAM assay, AGTR1 was stimulated with angiotensin II and showed increased on-plant vessel outgrowth. These results suggest candesartan does not negatively affect PPARγ or its target genes in human trophoblasts. More likely, candesartan from maternal serum may first act on fetal-placental AGTR1 and influence angiogenesis in the placenta, warranting further research. 相似文献
45.
Irina S. Panina Nikolay A. Krylov Anton O. Chugunov Roman G. Efremov Larisa V. Kordyukova 《International journal of molecular sciences》2022,23(23)
S-acylation is a post-translational linkage of long chain fatty acids to cysteines, playing a key role in normal physiology and disease. In human cells, the reaction is catalyzed by a family of 23 membrane DHHC-acyltransferases (carrying an Asp-His-His-Cys catalytic motif) in two stages: (1) acyl-CoA-mediated autoacylation of the enzyme; and (2) further transfer of the acyl chain to a protein substrate. Despite the availability of a 3D-structure of human acyltransferase (hDHHC20), the molecular aspects of lipid selectivity of DHHC-acyltransferases remain unclear. In this paper, using molecular dynamics (MD) simulations, we studied membrane-bound hDHHC20 right before the acylation by C12-, C14-, C16-, C18-, and C20-CoA substrates. We found that: (1) regardless of the chain length, its terminal methyl group always reaches the “ceiling” of the enzyme’s cavity; (2) only for C16, an optimal “reactivity” (assessed by a simple geometric criterion) permits the autoacylation; (3) in MD, some key interactions between an acyl-CoA and a protein differ from those in the reference crystal structure of the C16-CoA-hDHHS20 mutant complex (probably, because this structure corresponds to a non-native dimer). These features of specific recognition of full-size acyl-CoA substrates support our previous hypothesis of “geometric and physicochemical selectivity” derived for simplified acyl-CoA analogues. 相似文献
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47.
B. L. Boyce S. L. B. Kramer T. R. Bosiljevac E. Corona J. A. Moore K. Elkhodary C. H. M. Simha B. W. Williams A. R. Cerrone A. Nonn J. D. Hochhalter G. F. Bomarito J. E. Warner B. J. Carter D. H. Warner A. R. Ingraffea T. Zhang X. Fang J. Lua V. Chiaruttini M. Mazière S. Feld-Payet V. A. Yastrebov J. Besson J.-L. Chaboche J. Lian Y. Di B. Wu D. Novokshanov N. Vajragupta P. Kucharczyk V. Brinnel B. Döbereiner S. Münstermann M. K. Neilsen K. Dion K. N. Karlson J. W. Foulk A. A. Brown M. G. Veilleux J. L. Bignell S. E. Sanborn C. A. Jones P. D. Mattie K. Pack T. Wierzbicki S.-W. Chi S.-P. Lin A. Mahdavi J. Predan J. Zadravec A. J. Gross K. Ravi-Chandar L. Xue 《International Journal of Fracture》2016,198(1-2):5-100
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Larisa Dobircau Nicolas Delpouve Romuald Herbinet Sandra Domenek Loïc Le Pluart Laurent Delbreilh Violette Ducruet Eric Dargent 《Polymer Engineering and Science》2015,55(4):858-865
Molecular mobility and physical ageing of amorphous plasticized polylactide (PLA) with two different contents of mesolactide are studied with the help of thermal analysis. Used plasticizers are acetyl tributyl citrate (ATBC) and triacetin (TA), two molecules with established miscibility and plasticizing efficiency. Lower plasticizer permanence of TA compared with ATBC is found. The plasticizer molecules decreased the size of the cooperativity domains at the glass transition temperature Tg and likely in the glassy state by decreasing intermacromolecular interactions and notwithstanding the mesolactide content of PLA and the chemical identity of the plasticizer. The recovery function is given and shows a significant effect of the plasticizer on the physical ageing. The supplementary free volume brought by the plasticizer enhances molecular mobility in the glassy state and increases structural relaxation at one order of magnitude. The comparison between different plasticizers reveals that the structural relaxation is however independent from the type of plasticizer and the percentage of mesolactide in PLA. POLYM. ENG. SCI., 55:858–865, 2015. © 2014 Society of Plastics Engineers 相似文献