Monocyte diapedesis through an in vitro vessel wall construct: inhibition with monoclonal antibodies to thrombospondin

Human peripheral blood monocytes were examined for migration across an endothelial cell monolayer in an in vitro vessel wall construct. Few monocytes invaded in the absence of a chemotactic gradient, despite significant adhesion to the endothelial monolayer. However, the addition of zymosan-activated human plasma to the lower compartment, to create a chemotactic gradient across the vessel wall, resulted in significantly enhanced monocyte migration. Pretreatment of the monocytes with monoclonal antibodies to thrombospondin (TSP) dramatically inhibited monocyte diapedesis into the vessel wall. The same treatment inhibited monocyte adhesion to endothelial cells in two-dimensional monolayer cultures as well as in vessel wall constructs (no chemotactic gradient). Of interest, however, the monoclonal antibodies had no inhibitory effect on monocyte migration into collagen gels devoid of endothelial cells in response to the same chemotactic gradient, suggesting the importance of TSP in monocyte-endothelial cell interactions. Monoclonal antibodies to fibronectin and normal mouse immunoglobulin G did not inhibit migration in this model of a vessel wall. Furthermore, monoclonal antibodies to TSP showed no inhibition of human peripheral blood neutrophil migration. Previous studies have shown that monocytes synthesize TSP and express this moiety on their surface. The present data suggest that monocytes may utilize TSP to interact with endothelial cells lining the vessel wall during diapedesis.     

Numerische Simulation der Faserbewegung beim Strangpressen kurzfaserverstärkter Glasschmelzen

Numerical Simulation of the Fibre-Motion during the Extrusion of Short-Fibre-Reinforced Glass-Melts Fibre-reinforced materials are characterized by an anisotropic behaviour of the mechanical properties, which is caused by the alignment of the embedded fibres. In the case of short-fibre-compounds this behaviour is strongly influenced by the mechanism of flow during the manufacturing process. Numerical simulation Methods are preferentially used to get informations about the orientation of the reinforced fibres at the end of the molding-process and to improve the properties of the compound. For that, a model is developed, which calculates the motion of the short-fibres in the area of flow, basing on a three-dimensional finite-element-computation. Thereby the interaction between the Particular fibres during the orientation process is considered by using an interaction coefficient. Examplified at the extrusion of short fibre reinforced glass-melts, the fibre orientation is determined at models with different geometries of the pressing tool and variable boundary conditions. This procedure allows to determine the influence of the process-parameters On the expected quality of the composite. The represented simulation-model can also be used for other molding- and extrusion-processes of fibre reinforced materials.     

Highly purified omega-3-polyunsaturated fatty acids for topical treatment of psoriasis. Results of a double-blind, placebo-controlled multicentre study

We report the results of a multicentre, double-blind, placebo-controlled study of topical therapy with omega-3-polyunsaturated fatty acids (omega-3-PUFA) in 52 patients suffering from moderate plaque-type psoriasis. In each patient, two similar stable psoriatic plaques served as indicator lesions for the study. One indicator lesion was randomly assigned to treatment with topical preparations of highly purified omega-3-PUFA in one of two concentrations (1 or 10%), and the other was treated with placebo. Efficacy assessment was based on changes in local psoriasis severity index, area involved, erythema, desquamation, induration and pruritus. After 8 weeks of treatment, all indicator lesions had improved significantly, compared with baseline. However, no statistically or clinically relevant differences between the omega-3-PUFA-treated and the placebo-treated lesions were found. Therapy was well tolerated and, apart from one patient who developed perilesional eczema, no clinically relevant adverse events occurred. In conclusion, topical omega-3-PUFA were not effective in a randomized, placebo-controlled, double-blind setting. Results of non-blind trials should be (re-)considered with caution.     

Effects of task difficulty, goal setting, and strategy on performance of a heuristic task.

Investigated the relative contribution of goal setting and task difficulty to performance on a heuristic computer task with 128 undergraduates who attempted to solve either easy or difficult maze puzzles. Each S was assigned either an easy, moderate, or difficult goal or told to do his/her best. One month prior to the experiment, Ss responded to the Neuroticism scale of the Eysenck Personality Inventory to collect data on arousal. Data were also collected on acceptance, commitment, task complexity, and performance. Results show that both goals and task difficulty affected task performance, arousal, and perceptions of task complexity. A linear, rather than curvilinear, relationship was found between task arousal and performance. Contrary to prior research by G. A. Bassett (see record 1980-33518-001), results also show that, when the task was difficult, the setting of a difficult goal led to significantly lower performance. The decrease in performance in the difficult goal condition was attributed to the variation in performance strategy employed by these Ss as opposed to other Ss. It is argued that the setting of difficult goals may not be an effective motivational strategy when a heuristic, rather than algorithmic, solution is needed. (40 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Combined use of laparoscopic cholecystectomy and endoscopic retrograde cholangiography (ERC) and papillotomy (ERCP) in management of cholecysto-choledocholithiasis

In a review of 328 cases the combination of laparoscopic cholecystectomy with specific use of endoscopic retrograde cholangiography (pre- and postoperatively) proved to be a riskless, secure and time-saving procedure. In our opinion this is a minimal invasive method of treatment for patients with cholecysto-/choledocholithiasis.     

The distribution of SMN protein complex in human fetal tissues and its alteration in spinal muscular atrophy

Spinal muscular atrophy (SMA) is a common autosomal recessive neuromuscular disorder characterized by degeneration of motor neurons of the spinal cord and muscular atrophy. SMA is caused by alterations to the survival of motor neuron (SMN) gene, the function of which has hitherto been unclear. Here, we present immunoblot analyses showing that normal SMN protein expression undergoes a marked decay in the postnatal period compared with fetal development. Morphological and immunohistochemical analyses of the SMN protein in human fetal tissues showed a general distribution in the cytoplasm, except in muscle cells, where SMN protein was immunolocalized to large cytoplasmic dot-like structures and was tightly associated with membrane-free heavy sedimenting complexes. These cytoplasmic structures were similar in size to gem. The SMN protein was markedly deficient in tissues derived from type I SMA fetuses, including skeletal muscles and, as previously shown, spinal cord. While our data do not help decide whether SMA results from impaired SMN expression in spinal cord, skeletal muscle or both, they suggest a requirement for SMN protein during embryo-fetal development.