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71.
Polymerase Synthesis and Restriction Enzyme Cleavage of DNA Containing 7‐Substituted 7‐Deazaguanine Nucleobases 下载免费PDF全文
Michaela Mačková Soňa Boháčová Dr. Pavla Perlíková Dr. Lenka Poštová Slavětínská Prof. Dr. Michal Hocek 《Chembiochem : a European journal of chemical biology》2015,16(15):2225-2236
Previous studies of polymerase synthesis of base‐modified DNAs and their cleavage by restriction enzymes have mostly related only to 5‐substituted pyrimidine and 7‐substituted 7‐deazaadenine nucleotides. Here we report the synthesis of a series of 7‐substituted 7‐deazaguanine 2′‐deoxyribonucleoside 5′‐O‐triphosphates (dGRTPs), their use as substrates for polymerase synthesis of modified DNA and the influence of the modification on their cleavage by type II restriction endonucleases (REs). The dGRTPs were generally good substrates for polymerases but the PCR products could not be visualised on agarose gels by intercalator staining, due to fluorescence quenching. The presence of 7‐substituted 7‐deazaguanine residues in recognition sequences of REs in most cases completely blocked the cleavage. 相似文献
72.
Anton Trník Lenka Scheinherrová Tereza Kulovaná Pavel Reiterman Eva Vejmelková Robert Černý 《火与材料》2017,41(1):54-64
Clay shale is a specific type of material that contains a large amount of kaolinite. Burnt clay shale belongs to a large group of pozzolans, and its pozzolanic properties are activated after burning at temperatures similar to those when kaolinite is transformed into metakaolin. In this study, fine powder of burnt clay shale was used for the design of a high‐performance mortar as a partial replacement for portland cement up to 60 wt.%. The prepared specimens were subjected to a thermal analysis by using differential scanning calorimetry, thermogravimetry, and thermodilatometry. The investigation was performed in the temperature range 25–1000 °C. The basic physical and mechanical properties were studied as well. It was demonstrated that it is possible to design and produce a high‐performance mortar containing fine burnt clay shale powder and that an appropriate amount of this replacement is up to 20 wt.%. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
73.
Václav Čeřovský Dr. Miloš Buděšínský Dr. Oldřich Hovorka Dr. Josef Cvačka Dr. Zdeněk Voburka Jiřina Slaninová Dr. Lenka Borovičková Vladimír Fučík Dr. Lucie Bednárová Dr. Ivan Votruba Dr. Jakub Straka 《Chembiochem : a European journal of chemical biology》2009,10(12):2089-2099
Three novel structurally related pentadecapeptides, named lasioglossins, were isolated from the venom of the eusocial bee Lasioglossum laticeps. Their primary sequences were established as H‐Val‐Asn‐Trp‐Lys‐Lys‐Val‐Leu‐Gly‐Lys‐Ile‐Ile‐Lys‐Val‐Ala‐Lys‐NH2 (LL‐I), H‐Val‐Asn‐Trp‐Lys‐Lys‐Ile‐Leu‐Gly‐Lys‐Ile‐Ile‐Lys‐Val‐Ala‐Lys‐NH2 (LL‐II) and H‐Val‐Asn‐Trp‐Lys‐Lys‐Ile‐Leu‐Gly‐Lys‐Ile‐Ile‐Lys‐Val‐Val‐Lys‐NH2 (LL‐III). These lasioglossins exhibited potent antimicrobial activity against both Gram‐positive and Gram‐negative bacteria, low haemolytic and mast cell degranulation activity, and a potency to kill various cancer cells in vitro. The lasioglossin CD spectra were measured in the presence of trifluoroethanol and sodium dodecyl sulfate solution and indicated a high degree of α‐helical conformation. NMR spectroscopy, which was carried out in trifluoroethanol/water confirmed a curved α‐helical conformation with a concave hydrophobic and convex hydrophilic side. To understand the role of this bend on biological activity, we studied lasioglossin analogues in which the Gly in the centre of the molecule was replaced by other amino acid residues (Ala, Lys, Pro). The importance of the N‐terminal part of the molecule to the antimicrobial activity was revealed through truncation of five residues from both the N and C termini of the LL‐III peptide. C‐terminal deamidation of LL‐III resulted in a drop in antimicrobial activity, but esterification of the C terminus had no effect. Molecular modelling of LL‐III and the observed NOE contacts indicated the possible formation of a bifurcated H‐bond between hydrogen from the Lys15 CONH peptide bond and one H of the C‐terminal CONH2 to the Ile11 oxygen atom. Such interactions cannot form with C‐terminal esterification. 相似文献
74.
Zdeněk Chlup Hynek Hadraba Lenka Slabáková Daniel Drdlík Ivo Dlouhý 《Journal of the European Ceramic Society》2012,32(9):2057-2061
Laminates with strong bonds between thin layers were examined in this work to explore the influence of developed internal stresses on the fracture behaviour. A set of laminates having different level of internal stresses were prepared. Alumina and zirconia were the model materials for evenly alternating layers. The electrophoretic deposition technique was used for manufacturing of the laminates. The basic mechanical properties as elastic modulus and flexural strength were determined for all prepared materials. The crack propagation changes due to effect of internal stresses, elastic mismatch and surface effects were investigated using modified single edge notched beam technique. An extensive fractographical analysis of fracture surfaces was undertaken using laser confocal microscopy. The changes of the crack direction when crack propagates through alternating layers under different angels were described. Further, the effect of the internal stresses level within individual layers was reported. 相似文献
75.
Barancik M Bohacova V Gibalova L Sedlak J Sulova Z Breier A 《International journal of molecular sciences》2012,13(1):369-382
The drug efflux activity of P-glycoprotein (P-gp, a product of the mdr1 gene, ABCB1 member of ABC transporter family) represents a mechanism by which tumor cells escape death induced by chemotherapeutics. In this study, we investigated the mechanisms involved in the effects of pentoxifylline (PTX) on P-gp-mediated multidrug resistance (MDR) in mouse leukemia L1210/VCR cells. Parental sensitive mouse leukemia cells L1210, and multidrug-resistant cells, L1210/VCR, which are characterized by the overexpression of P-gp, were used as experimental models. The cells were exposed to 100 μmol/L PTX in the presence or absence of 1.2 μmol/L vincristine (VCR). Western blot analysis indicated a downregulation of P-gp protein expression when multidrug-resistant L1210/VCR cells were exposed to PTX. The effects of PTX on the sensitization of L1210/VCR cells to VCR correlate with the stimulation of apoptosis detected by Annexin V/propidium iodide apoptosis necrosis kit and proteolytic activation of both caspase-3 and caspase-9 monitored by Western blot analysis. Higher release of matrix metalloproteinases (MMPs), especially MMP-2, which could be attenuated by PTX, was found in L1210/VCR than in L1210 cells by gelatin zymography in electrophoretic gel. Exposure of resistant cells to PTX increased the content of phosphorylated Akt kinase. In contrast, the presence of VCR eliminated the effects of PTX on Akt kinase phosphorylation. Taken together, we conclude that PTX induces the sensitization of multidrug-resistant cells to VCR via downregulation of P-gp, stimulation of apoptosis and reduction of MMPs released from drug-resistant L1210/VCR cells. These facts bring new insights into the mechanisms of PTX action on cancer cells. 相似文献
76.
Critical evaluation of sequential extraction and sink-float methods used for the determination of Ga and Ge affinity in lignite 总被引:1,自引:0,他引:1
The affinities of Ga and Ge in lignite were determined using sequential extraction (SE) and element affinity calculation (EAC) based on sink-float data. For this study a bulk lignite sample was fractioned into two sets. The first set of samples (A) consisted of the different grain sizes fractions; the second one set (B) was prepared by density fractionation. Sequential extractions [1] were performed on both sets of fractions with very good agreement between determined organic elements affinities (OEA of Ga evaluated from A data is 32%, from B data 35%; OEA of Ge evaluated from A data is 31% and from B data 26%). The data of B lignite fractions were evaluated using two element affinity calculations: (a) EAC (I) of Klika and Kolomazník [2] and b) newly prepared subroutine EAC (II) based on quantitative contents of lignite macerals and minerals. There was also good agreement between both methods obtained (OEA of Ga calculated by EAC (I) is 83% and by EAC (II) 77%; OEA of Ge calculated by EAC (I) is 89% and by EAC (II) 97%). The significant differences of organic elements affinities of Ga and Ge evaluated by sequential extraction and by element affinity calculation based on sink-float data are discussed. 相似文献
77.
Ivana Fridrichova Lenka Kalinkova Sona Ciernikova 《International journal of molecular sciences》2022,23(20)
Metastatic breast cancer (MBC) is typically an incurable disease with high mortality rates; thus, early identification of metastatic features and disease recurrence through precise biomarkers is crucial. Circulating tumor cells (CTCs) consisting of heterogeneous subpopulations with different morphology and genetic, epigenetic, and gene expression profiles represent promising candidate biomarkers for metastatic potential. The experimentally verified role of epithelial-to-mesenchymal transition in cancer dissemination has not been clearly described in BC patients, but the stemness features of CTCs strongly contributes to metastatic potency. Single CTCs have been shown to be protected in the bloodstream against recognition by the immune system through impaired interactions with T lymphocytes and NK cells, while associations of heterotypic CTC clusters with platelets, leucocytes, neutrophils, tumor-associated macrophages, and fibroblasts improve their tumorigenic behavior. In addition to single CTC and CTC cluster characteristics, we reviewed CTC evaluation methods and clinical studies in early and metastatic BCs. The variable CTC tests were developed based on specific principles and strategies. However, CTC count and the presence of CTC clusters were shown to be most clinically relevant in existing clinical trials. Despite the known progress in CTC research and sampling of BC patients, implementation of CTCs and CTC clusters in routine diagnostic and treatment strategies still requires improvement in detection sensitivity and precise molecular characterizations, focused predominantly on the role of CTC clusters for their higher metastatic potency. 相似文献
78.
A number of resins were prepared by condensing m-aminoacetophenone with substituted aromatic compounds and formaldehyde in the presence of acids and bases as catalyst. The resins were characterized by infrared spectra. The solubility parameters were calculated from Small's group contribution which agreed well with the experimental value. The bacteriocidal properties of the resins have been studied. 相似文献
79.
80.
Rampichová M Martinová L Koštáková E Filová E Míčková A Buzgo M Michálek J Přádný M Nečas A Lukáš D Amler E 《Journal of materials science. Materials in medicine》2012,23(2):555-563
The structural properties of microfiber meshes made from poly(2-hydroxyethyl methacrylate) (PHEMA) were found to significantly
depend on the chemical composition and subsequent cross-linking and nebulization processes. PHEMA microfibres showed promise
as scaffolds for chondrocyte seeding and proliferation. Moreover, the peak liposome adhesion to PHEMA microfiber scaffolds
observed in our study resulted in the development of a simple drug anchoring system. Attached foetal bovine serum-loaded liposomes
significantly improved both chondrocyte adhesion and proliferation. In conclusion, fibrous scaffolds from PHEMA are promising
materials for tissue engineering and, in combination with liposomes, can serve as a simple drug delivery tool. 相似文献