基于自动测试系统的故障诊断专家系统

为在武器装备自动测试平台上建立故障诊断专家系统，介绍了一种新型的数据库访问技术ADO。作为实例，在自动测试平台上利用ADO技术实现了测试结果的读取，将测试数据作为故障诊断的入口参数，结合SQL语言在规则库中实现了推理机制，最后，用Visual C 语言实现了编程。     

基于迁移策略的分布式遗传算法多用户检测器

针对简单遗传算法多用户检测器的收敛速度慢和“早熟”问题,利用解相关算法的抗多址干扰能力和分布式遗传算法的快速全局寻优优势,提出了一种基于迁移策略的分布式遗传算法解相关多用户检测器。计算机仿真结果表明:该多用户检测器的检测性能明显优于简单遗传算法多用户检测器和解相关多用户检测器,更易于实现。     

Vague集的模糊熵和距离测度的相互诱导关系

Vague集自提出以来,由于它在各个领域中的广泛应用而引起众多学者的注意,而模糊熵和距离测度是其中的关键技术。目前已有多种Vague集的模糊熵和距离测度的计算方法被提出来,但所有这些研究都没有讨论两者之间的联系。论文基于Vague集的模糊熵和距离测度的公理化定义,给出两者之间的相互诱导关系,建立了模糊熵和距离测度之间的联系。     

基于CPU和GPU异构的蛋白质分子半柔性对接算法优化

分子对接是预测蛋白质复合物的有效手段。对于分子对接算法的优化旨在加速分子对接效率,降低计算成本,以及充分发挥计算资源的利用率。本文主要采用3个方案对半柔性对接算法进行优化：（1）方案一在CPU端进行优化;（2）方案二在方案一的基础上,利用CUFFT的移植工具CUFFTW为方案一提供部分GPU并行接口;（3）方案三利用GPU并行架构,通过CPU和GPU的协同处理,利用纯并行计算接口进行优化。3种方案对PDB code分别为1PEE,1B6C,4HX3和2SNI的测试蛋白进行结合态和自由态的对接,求得的最小均方根偏差L_RMSD小于5 Å,满足了复合物结构预测竞赛要求的中等精度结构标准,验证了对接结果的正确性。最后在保证结果正确性的前提下,测试了不同蛋白在不同方案下的运行速率;在保证不同蛋白对接效率相同的前提下,以1PPE为例,比较了不同方案下的对接速率。实验结果表明在同等旋转步长并保证程序运行结果正确性的前提下,最终的优化效果可提速近10倍,有效改进了半柔性对接算法的运行速率。     

The Use of Distinctive Monoclonal Antibodies in FMD VLP- and P1-Based Blocking ELISA for the Seromonitoring of Vaccinated Swine

The serum neutralization (SN) test has been regarded as the “gold standard” for seroconversion following foot-and-mouth disease virus (FMDV) vaccination, although a high-level biosafety laboratory is necessary. ELISA is one alternative, and its format is constantly being improved. For instance, standard polyclonal antisera have been replaced by monoclonal antibodies (MAbs) for catching and detecting antibodies, and inactive viruses have been replaced by virus-like particles (VLPs). To the best of current knowledge, however, no researchers have evaluated the performances of different MAbs as tracers. In previous studies, we successfully identified site 1 and site 2 MAbs Q10E and P11A. In this study, following the established screening platform, the VLPs of putative escape mutants from sites 1 to 5 were expressed and used to demonstrate that S11B is a site 3 MAb. Additionally, the vulnerability of VLPs prompted us to assess another diagnostic antigen: unprocessed polyprotein P1. Therefore, we established and evaluated the performance of blocking ELISA (bELISA) systems based on VLPs and P1, pairing them with Q10E, P11A, S11B, and the non-neutralizing TSG MAb as tracers. The results indicated that the VLP paired with S11B demonstrated the highest correlation with the SN titers (R² = 0.8071, n = 63). Excluding weakly positive serum samples (SN = 16–32, n = 14), the sensitivity and specificity were 95.65% and 96.15% (kappa = 0.92), respectively. Additionally, the P1 pairing with Q10E also demonstrated a high correlation (R² = 0.768). We also discovered that these four antibodies had steric effects on one another to varying degrees, despite recognizing distinct antigenic sites. This finding indicated that MAbs as tracers could not accurately detect specific antibodies, possibly because MAbs are bulky compared to a protomeric unit. However, our results still provide convincing support for the application of two pairs of bELISA systems: VLP:S11B-HRP and P1:Q10E-HRP.     

电网调度侧一次调频在线监测系统的开发与应用

介绍并网运行机组一次调频功能的相关规定、控制回路、投运中存在的问题以及常规检测手段。针对常规检测手段在实时性和连续性方面的不足,提出电网调度侧一次调频在线监测系统的技术方案。该方案通过信号的接收监测、数据的分析处理等步骤,可实现电网调度侧对一次调频投运情况的在线监测及分析评价,并可以报表的形式输出,供调度人员参考。     

紫外法和皮粉法测定塔拉单宁含量的对比研究

分别采用紫外法和皮粉法对5种不同含量的塔拉单宁进行对比研究,并利用SPSS软件对单宁含量测定结果进行显著性检验,结果表明：高纯度塔拉单宁(93%)分别用紫外法和皮粉法测定单宁含量时实验结果并未显著差异(P>0.05),而4种纯度为60%左右塔拉单宁的实验结果均具有显著性差异(P<0.05),皮粉法测得的单宁含量均比用紫外法测得的结果高,差值约为1.5%～2.0%,并由此计算出紫外法测定塔拉单宁含量计算公式中的矫正常数p为1.03。紫外-可见吸收光谱与HPLC分析结果表明：塔拉单宁与五倍子单宁的最大吸收峰均为276 nm,同质量浓度下塔拉单宁的吸收峰强度高于五倍子单宁,塔拉单宁成分出峰时间主要在20～40 min,五倍子单宁主要在30～45 min。     

基于J2ME和J2EE的城市公交查询系统的设计与实现

J2ME和J2EE技术是目前Java两大主要技术,并在应用中取得了成功。本文基于J2ME和J2EE技术实现了一个移动手机版的城市公交查询系统。其中,J2ME技术拥有移动手机客户端的开发,而J2EE技术则用于后台服务器和手机客户端的交互,以及后台服务器信息的发布。本系统实现了三种查询功能：路线查询、站点查询和站站查询。通过本系统将极大地方便手机用户对公交信息的查询。     

Characterization of Inhibitory Capability on Hyperpolarization-Activated Cation Current Caused by Lutein (β,ε-Carotene-3,3′-Diol), a Dietary Xanthophyll Carotenoid

Lutein (β,ε-carotene-3,3′-diol), a xanthophyll carotenoid, is found in high concentrations in the macula of the human retina. It has been recognized to exert potential effectiveness in antioxidative and anti-inflammatory properties. However, whether and how its modifications on varying types of plasmalemmal ionic currents occur in electrically excitable cells remain incompletely answered. The current hypothesis is that lutein produces any direct adjustments on ionic currents (e.g., hyperpolarization-activated cation current, I_h [or funny current, I_f]). In the present study, GH₃-cell exposure to lutein resulted in a time-, state- and concentration-dependent reduction in I_h amplitude with an IC₅₀ value of 4.1 μM. There was a hyperpolarizing shift along the voltage axis in the steady-state activation curve of I_h in the presence of this compound, despite being void of changes in the gating charge of the curve. Under continued exposure to lutein (3 μM), further addition of oxaliplatin (10 μM) or ivabradine (3 μM) could be effective at either reversing or further decreasing lutein-induced suppression of hyperpolarization-evoked I_h, respectively. The voltage-dependent anti-clockwise hysteresis of I_h responding to long-lasting inverted isosceles-triangular ramp concentration-dependently became diminished by adding this compound. However, the addition of 10 μM lutein caused a mild but significant suppression in the amplitude of erg-mediated or A-type K⁺ currents. Under current-clamp potential recordings, the sag potential evoked by long-lasting hyperpolarizing current stimulus was reduced under cell exposure to lutein. Altogether, findings from the current observations enabled us to reflect that during cell exposure to lutein used at pharmacologically achievable concentrations, lutein-perturbed inhibition of I_h would be an ionic mechanism underlying its changes in membrane excitability.     

Therapeutic Effects of Insulin-Producing Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Type 1 Diabetes Mouse Model

In patients with type 1 diabetes (T1D), compromised pancreatic β-cell functions are compensated through daily insulin injections or the transplantation of pancreatic tissue or islet cells. However, both approaches are associated with specific challenges. The transplantation of mesenchymal stem cells (MSCs) represents a potential alternative, as MSCs have tissue-forming capacity and can be isolated from various tissues. The human umbilical cord (hUC) is a good source of freely available MSCs, which can be collected through pain-free, non-invasive methods subject to minimal ethical concerns. We sought to develop a method for the in vitro generation of insulin-producing cells (IPCs) using MSCs. We examined the potential therapeutic uses and efficacy of IPCs generated from hUC-derived MSCs (hUC-IPCs) and human adipose tissue (hAD)-derived MSCs (hAD-IPCs) through in vitro experiments and streptozotocin (STZ)-induced C57BL/6 T1D mouse models. We discovered that compared to hAD-IPCs, hUC-IPCs exhibited a superior insulin secretion capacity. Therefore, hUC-IPCs were selected as candidates for T1D cell therapy in mice. Fasting glucose and intraperitoneal glucose tolerance test levels were lower in hUC-IPC-transplanted mice than in T1D control mice and hAD-IPC-transplanted mice. Our findings support the potential use of MSCs for the treatment of T1D.