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991.
This analysis uses data from a survey of Toronto commuter cyclists that collected information regarding accident history as well as regular commute route to work or school. By relating the route information of the 1196 respondents to facility attributes in a Geographic Information System (GIS), defensible estimates of travel exposure on roads, off-road paths and sidewalks were developed. The rate of collision on off-road paths and sidewalks was lower than for roads. The relative rates for falls and injuries suggest these events are least common on-road followed by off-road paths, and finally most common on sidewalks. The rate of major injuries, an injury that required medical attention, was greatest on sidewalks and the difference between paths and sidewalks was negligible. These rates suggest a need for detailed analysis of sidewalk and off-road path bicycle safety. The absolute event rates per bicycle kilometer were found to be between 26 and 68 times higher than similar rates for automobile travel, re-confirming the urgent bicycle safety crisis. Examination of rates for sub-groups of cyclists suggest that experience is an important factor in bicycle safety. The same survey conducted in Ottawa, Canada found event rates much lower than Toronto. This result may confirm urban form, traffic levels and attitude do affect bicycle safety. The analysis also demonstrates a successful method to quantify bicycle travel exposure information and should be considered for further use as complement to other existing techniques. 相似文献
992.
Rosemary Westcott Natalie Chung Arnab Ghosh Lisa Ferguson William Bingaman Imad M. Najm Chaitali Ghosh 《International journal of molecular sciences》2022,23(9)
The glucocorticoid receptor (GR) at the blood–brain barrier (BBB) is involved in the pathogenesis of drug-resistant epilepsy with focal cortical dysplasia (FCD); however, the roles of GR isoforms GRα and GRβ in the dysplastic brain have not been revealed. We utilized dysplastic/epileptic and non-dysplastic brain tissue from patients who underwent resective epilepsy surgery to identify the GRα and GRβ levels, subcellular localization, and cellular specificity. BBB endothelial cells isolated from the dysplastic brain tissue (EPI-ECs) were used to decipher the key BBB proteins related to drug regulation and BBB integrity compared to control and transfected GRβ-overexpressed BBB endothelial cells. GRβ was upregulated in dysplastic compared to non-dysplastic tissues, and an imbalance of the GRα/GRβ ratio was significant in females vs. males and in patients > 45 years old. In EPI-ECs, the subcellular localization and expression patterns of GRβ, Hsp90, CYP3A4, and CYP2C9 were consistent with GRβ+ brain endothelial cells. Active matrix metalloproteinase levels and activity increased, whereas claudin-5 levels decreased in both EPI-ECs and GRβ+ endothelial cells. In conclusion, the GRβ has a major effect on dysplastic BBB functional proteins and is age and gender-dependent, suggesting a critical role of brain GRβ in dysplasia as a potential biomarker and therapeutic target in epilepsy. 相似文献
993.
VLSI design has caused a revolution in microminiaturization. The design process requires construction of stick diagrams for the digital circuit to be designed. These diagrams are prepared using different colored layers. The color graphics and the color monitors make the overall system quite expensive. The object of this article is to show how an inexpensive personal computer like the Apple MacIntosh can be used for preparing VLSI stick diagrams. The Apple MacIntosh's capabilities include translation, mirroring, detail work and hatching; which are indispensible in the construction of stick diagrams. The stick diagrams for a few examples such as serial adder, half adder, adder/subtractor and generalized pipeline array cell have been worked out. It is hoped that this paper will aid in making VLSI design easier on less expensive and easily obtainable personal computers. 相似文献
994.
Alba N. Mininni Caterina Pellizzari Barbara Cardazzo Lisa Carraro Stefania Balzan Enrico Novelli 《International Dairy Journal》2009,19(10):617-623
Many typical Italian cheeses made from ovine milk are certified as Protected Designation of Origin (PDO). Because caprine and ovine milk production is limited, the fraudulent addition of cows' milk is widespread. In addition, some compounds in bovine milk have high allergenic potential; therefore, such fraud also has implications for consumer health. In this study, a real-time polymerase chain reaction (real-time PCR) test was developed to detect and quantify cow's milk in caprine and ovine cheeses, based on two target genes. The mitochondrial Cytochrome b gene (Cytb) of Bos taurus was used to detect and quantify bovine DNA. The nuclear gene myostatin (Myo), nuclear ribosomal gene 18S, or mitochondrial gene 16S were used alternatively as universal reference markers. Caprine (n = 30) and ovine (n = 51) cheese samples were purchased and analyzed and most were shown to be contaminated by bovine milk. Pairwise analysis of quantification data using a Spearmann Rank Correlation test demonstrated a highly significant correlation between data obtained with the different reference assays. 相似文献
995.
Listeria monocytogenes is a widespread foodborne pathogen that represents a major concern with respect to food safety. Rapid identification of this bacterium at a subspecies level is important to trace back an outbreak and improve risk-based inspection programs. A method for subtyping L. monocytogenes at the serotype and haplotype levels was developed using Fourier transform infrared (FT-IR) reflectance microscopy. Thirty strains of L. monocytogenes belonging to four different PCR serotypes (1/2a, 1/2b, 4b, and 4c) that had previously been characterized by Multilocus genotyping (MLGT) and Pulsed field gel electrophoresis (PFGE) assays were used in this study. The FT-IR based identification and classification was compared to the known MLGT and PFGE subtyping of the L. monocytogenes. Canonical variate analysis (CVA) of the spectra resulted in 96.6% correct identification of L. monocytogenes at the serotype level. Hierarchical cluster analysis (HCA) and CVA of the spectra showed 91.7% correct identification of strains at the haplotype level consistent with their MLGT groupings. FT-IR spectra of strains were also differentiated correctly in accordance with their PFGE haplotyping. Additionally, by using HCA of FT-IR spectra, each bacterium was differentiated at the strain level. Starting from a pure culture, this method enabled classification of L. monocytogenes at the serotype, haplotype, and/or strain level within 18 h, which is faster and potentially less expensive than the molecular methods and previous FT-IR methods. This is the first report of the identification of L. monocytogenes at the haplotype level using FT-IR. 相似文献
996.
Lisa Cangioli Marco Mancini Marco Napoli Camilla Fagorzi Simone Orlandini Francesca Vaccaro Alessio Mengoni 《International journal of molecular sciences》2022,23(7)
The taxonomic assemblage and functions of the plant bacterial community are strongly influenced by soil and host plant genotype. Crop breeding, especially after the massive use of nitrogen fertilizers which led to varieties responding better to nitrogen fertilization, has implicitly modified the ability of the plant root to recruit an effective bacterial community. Among the priorities for harnessing the plant bacterial community, plant genotype-by-microbiome interactions are stirring attention. Here, we analyzed the effect of plant variety and fertilization on the rhizosphere bacterial community. In particular, we clarified the presence in the bacterial community of a varietal effect of N and P fertilization treatment. 16S rRNA gene amplicon sequence analysis of rhizospheric soil, collected from four wheat varieties grown under four N-P fertilization regimes, and quantification of functional bacterial genes involved in the nitrogen cycle (nifH; amoA; nirK and nosZ) were performed. Results showed that variety played the most important role and that treatments did not affect either bacterial community diversity or bacterial phyla abundance. Variety-specific response of rhizosphere bacterial community was detected, both in relation to taxa (Nitrospira) and metabolic functions. In particular, the changes related to amino acid and aerobic metabolism and abundance of genes involved in the nitrogen cycle (amoA and nosZ), suggested that plant variety may lead to functional changes in the cycling of the plant-assimilable nitrogen. 相似文献
997.
Sara R. Jaeger Jayson L. Lusk Lisa O. House Carlotta Valli Melissa Moore Bert Morrow W. Bruce Traill 《Food quality and preference》2004,15(7-8):701-714
The findings from a study measuring consumer acceptance of genetically modified (GM) foods are presented. The empirical data were collected in an experimental market, an approach used extensively in experimental economics for measuring the monetary value of goods. The approach has several advantages over standard approaches used in sensory and marketing research (e.g., surveys and focus groups) because of its non-hypothetical nature and the realism introduced by using real goods, real money, and market discipline. In each of three US locations, we elicited the monetary compensation consumers required to consume a GM food. Providing positive information about the benefits of GM food production, in some cases, reduced the level of monetary compensation demanded to consume the GM food. 相似文献
998.
Mather ML Crowe JA Morgan SP White LJ Kalashnikov AN Ivchenko VG Howdle SM Shakesheff KM 《Journal of materials science. Materials in medicine》2008,19(9):3071-3080
Polymeric tissue scaffolds are central to many regenerative medicine therapies offering a new approach to medicine. As the number of these regenerative therapies increases there is a pressing need for an improved understanding of the methods of scaffold fabrication. Of the many approaches to processing scaffolds, supercritical fluid fabrication methods have a distinct advantage over other techniques as they do not require the use of organic solvents, elevated processing temperatures or leaching processes. The work presented here is centred on the development of a new approach to monitoring supercritical scaffold fabrication based on determination of the scaffold acoustic impedance to inform protocols for scaffold fabrication. The approach taken uses an ultrasonic pulse-echo reflectometer enabling non-invasive monitoring of the supercritical environment on-line. The feasibility of this approach was investigated for two scaffolds of different molecular weight. Acoustic results demonstrate that differences in the physical properties of the two scaffolds could be resolved, particularly during the foaming process which correlated with findings from time-lapsed imaging and micro X-ray computed tomography (micro X-ray CT) images. Thus, this work demonstrates the feasibility of ultrasonic pulse-echo reflectometry to non-invasively study supercritical scaffold fabrication on-line providing a greater understanding of the scaffold fabrication process. 相似文献
999.
Lisa M. Vizer Lina Zhou Andrew Sears 《International journal of human-computer studies》2009,67(10):870-886
Monitoring of cognitive and physical function is central to the care of people with or at risk for various health conditions, but existing solutions rely on intrusive methods that are inadequate for continuous tracking. Less intrusive techniques that facilitate more accurate and frequent monitoring of the status of cognitive or physical function become increasingly desirable as the population ages and lifespan increases. Since the number of seniors using computers continues to grow dramatically, a method that exploits normal daily computer interactions is attractive. This research explores the possibility of detecting cognitive and physical stress by monitoring keyboard interactions with the eventual goal of detecting acute or gradual changes in cognitive and physical function. Researchers have already attributed a certain amount of variability and “drift” in an individual's typing pattern to situational factors as well as stress, but this phenomenon has not been explored adequately. In an attempt to detect changes in typing associated with stress, this research analyzes keystroke and linguistic features of spontaneously generated text. Results show that it is possible to classify cognitive and physical stress conditions relative to non-stress conditions based on keystroke and linguistic features with accuracy rates comparable to those currently obtained using affective computing methods. The proposed approach is attractive because it requires no additional hardware, is unobtrusive, is adaptable to individual users, and is of very low cost. This research demonstrates the potential of exploiting continuous monitoring of keyboard interactions to support the early detection of changes in cognitive and physical function. 相似文献
1000.
Designers of distributed algorithms typically assume strong memory consistency guarantees, but system implementations provide weaker guarantees for better performance and scalability. This motivates the study of how to implement programs designed for sequential consistency on platforms with weaker consistency models. Typically, such implementations are impossible using only read and write operations to shared variables. One variant of processor consistency originally proposed by Goodman and called here PC-G is an exception because it provides just enough consistency to implement mutual exclusion using only reads and writes. This paper investigates the existence of compilers to convert arbitrary programs that use shared read/write variables with sequentially consistent memory semantics, to programs that use read/write variables with PC-G consistency semantics. We first provide a simple program transformation, and prove that it correctly compiles any 2-process program to a PC-G memory system, while preserving wait-freedom. We next prove that even a substantial generalization of this transformation cannot be a compiler for even a very restricted class of 3-process programs. Even though our program transformation is not a general compiler for three or more processes, it does correctly transform some specific n-process programs. In particular, for the special case of the (necessarily randomized) Test&Set algorithm of Tromp and Vitanyi, our transformation extends to any number of processes, thus providing the first algorithm for expected wait-free Test&Set on any weak memory system, using only read/write variables. 相似文献