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41.
Jinguo Huang Ruiqi Zhao Jing Mo Fang Wang Dr. Xiaocheng Weng Xiang Zhou 《Chembiochem : a European journal of chemical biology》2021,22(9):1559-1562
There is growing interest in developing intracellular RNA tools. Herein, we describe a strategy for N3-kethoxal (N3K)-based bioorthogonal intracellular RNA functionalization. With N3K labeling followed by an in vivo click reaction with DBCO derivatives, RNA can be modified with fluorescent or phenol groups. This strategy provides a new way of labeling RNA inside cells. 相似文献
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Y. H. Cheng X. L. Qiao J. G. Chen Y. P. Wu C. S. Xie Y. Q. Wang D. S. Xu S. B. Mo Y. B. Sun 《Surface & coatings technology》2002,160(2-3)
Carbon nitride films were deposited by direct current plasma assisted pulsed laser ablation of a graphite target under a nitrogen atmosphere at room temperature. The surface morphology, composition and bonding structure of the deposited films were characterized by atomic force microscopy (AFM), Fourier transform infrared (FTIR) spectroscopy, Raman, and X-ray photoelectron spectroscopy (XPS). The effect of laser fluences in the range 0.5–3 J/cm2 on the surface morphology, composition and bonding structure of the carbon nitride films were systematically studied. As laser fluence is increased, AFM results show a great decrease in the surface roughness of carbon nitride films. FTIR and XPS results indicate an increase in the N/C ratio and the content of N atoms bonded to sp3 C, as well as a decrease in the content of H atoms and the content of N atoms boned to sp2 C in the deposited films, and Raman spectra indicate an increase in the content of disordered sp2 C atoms and the sp2 cluster size. The increase in the film density and the decrease in the particle fraction contribute to the decrease of surface roughness with increasing laser fluence. 相似文献
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首先介绍了逆向工程技术与传统制造技术的区别,然后着重讨论利用逆向工程技术快速制造复杂铸造模具的过程及工艺路线,同时给出一些具体应用实例。最后得出与传统制造方法相比具有效率高、成本低、操作简单等优点的结论。 相似文献
45.
测定了庚烯-(3)的蒸气压。用Gillespie型平衡器测定了甲苯—庚烯-(3)、正庚烷—庚烯-(3)二元系及甲苯—正庚烷—庚烯-(3)三元系统的平衡数据,并分别用Spinner及White方法验证了这些数据,计算结果与实验结果是符合的。三元系统气液平衡的数据不论在工程中或是实验工作上都很重要,过去对三元系统气液平衡的研究虽然有一些,但对甲苯—正庚烷—庚烯-(3)三元系统气液平衡至今在文献上尚未有记载。 由于庚烯-(3)的蒸气压数据不见于文献,我们测定了它的蒸气压数据。正庚烷—甲苯二元系统前人已有许多结果发表,我们进行了一些校核,结果与文献记载符合。甲苯—庚烯-(3)二元系统文献上没有,我们倣效了并用Spinner方法及White方法整理。至于正庚烷—庚烯-(3)二元系统,因为此两组分的性质(如沸点、n_D~(20)等)极其接近,分析误差较大,我们将其当作理想溶液处理,误差当在允许误差以内。 三元系统的分析较为繁难,误差也较大,故希望从理论和实验材料找出其规律性,以校核实验数据和推算未知三元系统。本工作利用三个二元系统的数据,用Spinner以及White的方法推算三元系统的气液平衡,与实验数据相比较,是相互符合的。 相似文献
46.
聚铝溶液pH值及盐基度的相关性研究 总被引:6,自引:1,他引:6
为聚铝溶液进行了PH值和盐基度的分析和估算,给出了聚铝溶液的极限PH值和极限盐基度与总溶解铝浓度的关系曲线,最终计算结果表明:在平衡条件下,聚铝溶液的PH值和盐基度具有相关性,即当基中一个参数发生变化时。另一个参数必然要发生相应的改变。 相似文献
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MOLECULAR PRINCIPLE OF CORRESPONDING STATES FOR VISCOSITY AND THERMAL CONDUCTIVITY OF FLUID MIXTURES
Conformal solution theory is developed for the viscosity and thermal conductivity of fluid mixtures. The procedure involves expanding the transport coefficient for the mixture about the value for an ideal solution, using groupings of the potential parameters and molecular mass as expansion coefficients. The parameters for the ideal solution are chosen so as to annul the first-order term in this expansion, thus encouraging rapid convergence. This yields mixing rules (similar to those of the van der Waals 1 theory for thermodynamic properties) for the potential parameters and molecular mass of the reference fluid. Reference fluid properties are obtained from pure fluid corresponding states correlations
By making calculations for dilute gas mixtures and comparing with Chapman-Enskog theory, it is found that the first-order theory works well for mixtures of quite widely different energy parameters (ε) and molecular masses; it is more sensitive to the size difference of the molecular components, however. For cryogenic liquid mixtures composed of simple liquids good results are obtained using two-parameter corresponding states for the reference fluid. For polyatomic fluids it is necessary to use a three-parameter corresponding states approach for the pure fluids. A method of introducing a third parameter, while retaining the simplicity of having only two independent variables, is used for such fluids. Good results are obtained for a variety of binary mixtures. The method is of particular value for multicomponent fluids. Thus, without fitting any parameters from ternary data the theory predicts viscosities for the system carbon tetrachloride/n-hexane/benzene over the full composition range with a standard deviation of only 1.69%. 相似文献
By making calculations for dilute gas mixtures and comparing with Chapman-Enskog theory, it is found that the first-order theory works well for mixtures of quite widely different energy parameters (ε) and molecular masses; it is more sensitive to the size difference of the molecular components, however. For cryogenic liquid mixtures composed of simple liquids good results are obtained using two-parameter corresponding states for the reference fluid. For polyatomic fluids it is necessary to use a three-parameter corresponding states approach for the pure fluids. A method of introducing a third parameter, while retaining the simplicity of having only two independent variables, is used for such fluids. Good results are obtained for a variety of binary mixtures. The method is of particular value for multicomponent fluids. Thus, without fitting any parameters from ternary data the theory predicts viscosities for the system carbon tetrachloride/n-hexane/benzene over the full composition range with a standard deviation of only 1.69%. 相似文献
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通过厂房地下洞室群围岩点荷载试验,获得了洞室不同深度位置岩石点荷载强度值,试验发现各平洞点荷载强度随洞室深度变化呈现明显的波动性变化特征。研究表明,围岩岩性、断层以及岩石微观结构等是影响岩石点荷载强度的主要因素。其中,围岩中普遍发育的矿物定向排列是导致点荷载强度波动性变化的最主要因素。最后,通过与实测单轴抗压强度比较,得出当岩石单轴抗压强度大于60 MPa时,宜采用成都理工大学推荐公式换算单轴抗压强度,当单轴抗压强度小于60 MPa时,宜采用水利水电规范推荐的转换公式计算岩石单轴抗压强度。 相似文献