The E3 Ligase GmPUB21 Negatively Regulates Drought and Salinity Stress Response in Soybean

E3-ubiquitin ligases are known to confer abiotic stress responses in plants. In the present study, GmPUB21, a novel U-box E3-ubiquitin ligase-encoding gene, was isolated from soybean and functionally characterized. The expression of GmPUB21, which possesses E3-ubiquitin ligase activity, was found to be significantly up-regulated by drought, salinity, and ABA treatments. The fusion protein GmPUB21-GFP was localized in the cytoplasm, nucleus, and plasma membrane. Transgenic lines of the Nicotiana benthamiana over-expressing GmPUB21 showed more sensitive to osmotic, salinity stress and ABA in seed germination and inhibited mannitol/NaCl-mediated stomatal closure. Moreover, higher reactive oxygen species accumulation was observed in GmPUB21 overexpressing plants after drought and salinity treatment than in wild-type (WT) plants. Contrarily, silencing of GmPUB21 in soybean plants significantly enhanced the tolerance to drought and salinity stresses. Collectively, our results revealed that GmPUB21 negatively regulates the drought and salinity tolerance by increasing the stomatal density and aperture via the ABA signaling pathway. These findings improved our understanding of the role of GmPUB21 under drought and salinity stresses in soybean.     

Transient simulation and analysis of the simultaneous load rejection process in pumped storage power stations using a 1-D-3-D coupling method

The load rejection imposes a danger in the pumped storage hydropower plants (PSPs),especially when two or more pump turbines reject their loads simultaneously.I...     

Improving the precision of optical metrology by detecting fewer photons with biased weak measurement

In optical metrological protocols to measure physical quantities, it is, in principle, always beneficial to increase photon number n to improve measurement prec...     

沥青路面弯道施工技术浅议

由于受摊铺机找平原理、找平基准设置、自动找平延时特性以及人为的误操作等因素的影响,即便装有微电脑控制自动找平装置的摊铺机,摊铺后的沥青路面还是不太理想.结合实际施工工作,从道路结构特点、施工工艺、施工机械、控制系统等8个方面定性地总结了影响沥青路面弯道标高准确性与平整度的因素,并提出从优化施工组织、基线找准、控制信号调整、压路机碾压等4个方面提高弯道路面沥青的摊铺质量.     

PLC应用中的问题及解决办法

就教学及实践工作中遇到的一些典型问题做一探讨,以期对正在学习和应用PLC的人员有所帮助.     

Internal OH? induced cascade quenching of upconversion luminescence in NaYF4:Yb/Er nanocrystals

Internal hydroxyl impurity is known as one of the main detrimental factors affecting the upconversion (UC) efficiency of upconversion luminescence (UCL) nanomat...     

基于DSP的嵌入式系统的程序引导设计

作对以DSP为核心的嵌入式系统的程序引导功能进行了深入的研究及分析,并以TI公司的TMS320C33芯片为实例,归纳了其实现流程及设计方案,从硬件和软件两个方面阐述了实现该功能的关键点和值得注意的地方。该设计已被应用于一个配电网环网柜智能终端装置。运行实践表明,是一种简便、可靠的方案。     

变电站接地网大修改造探讨

变电站接地网改造设计是一项实践性很强的工作,针对兴义供电局开展的系列变电站地网大修改造工程。对地网安全指标评估、土壤结构分析、地网安全性能仿真研究、改造设计方法及施工工艺等实践经验进行了总结,旨在为今后相关单位的类似工作提供必要的参考。     

空芯螺管型超导储能磁体的优化设计方法

微、小型超导储能系统由于运行操作灵活性和制造工艺简单而在电力系统中具有广泛的应用前景。作为超导储能系统的关键部件,储能超导磁体的优化设计,不仅应从技术上保证超导储能系统运行的安全可靠性,而且应能最大限度地降低制造成本。本文针对超导储能磁体系统的基本组成单元－螺管型超导磁体,从磁体绕组的基本参数如绕组厚度、径高比的变化出发,分析了螺管型储能超导磁体的储能效率,提出了螺管型超导储能磁体的优化设计方法。     

钛酸钡添加量对埋容用环氧-钛酸钡-玻璃布板材性能的影响

采用热固化层压方法制备了环氧-钛酸钡-玻璃布(EBG)板材,并分析钛酸钡(BTO)添加量对埋容用EBG板材各项性能的影响。结果表明:随着BTO填料添加量的增大,EBG板材的实际密度先接近后偏离理论密度,孔隙率先减小后增大,耐浸焊时间、剥离强度先增大后减小,5%热分解温度和介电常数增大;当BTO的质量分数为80%左右时,埋容用EBG板材具有较好的综合性能;EBG板材为一种混联模型。