Genetic mapping of the spinocerebellar ataxia type 2 gene on human chromosome 12

The dominant spinocerebellar ataxias are a genetically heterogeneous group of diseases leading to premature death of neurons in the cerebellum and other parts of the nervous system. The mutation causing SCA1 is on human chromosome (CHR) 6p and SCA3 is on CHR 14q. To refine the location of the SCA2 gene on CHR 12q, we performed genetic linkage analysis between the SCA2 locus and nine Ioci (D12S58, D12S78, D12S317, D12S330, D12S353, D12S84, D12S105, D12S79, and PLA2) in three SCA2 families. The highest pairwise lod scores were obtained between SCA2 and D12S84/D12S105 and D12S79. We determined the best order and genetic distances among these loci in ten multigenerational families by multipoint linkage analysis and established the following order: D12S101-D12S58/IGF1- D12S78-D12S317-D12S330/D12S353-D12S84/D 12S105-D12S79-PLA2. Using this genetic map, multipoint linkage analysis placed SCA2 between D12S84/D12S105 and D12S79.     

Impaired endothelin-1 release in tilt-induced syncope

BACKGROUND: Nitric oxide (NO) is considered to be one of the endogenous inhibitory factors of ischemic reperfusion injury. In this study, the NO-producing ability of the preserved lung, flushed at various pulmonary artery pressures (flushing pressure), was studied during reperfusion using an ex vivo rabbit lung perfusion model. METHODS: The lungs were flushed with 200 ml of preservation solution with flushing pressures adjusted to 15, 15, 20, and 25 mmHg for groups 1, 2, 3, and 4, respectively (n=5 in each group). In the control group (group 1), the heart-lung block was harvested after flushing and the lungs were assessed without preservation. In the other groups, the harvested blocks were preserved at 8 degrees C for 24 hr and reperfused with homologous blood for pulmonary functional assessment. Pulmonary function was assessed by measuring mean airway pressure, mean pulmonary arterial pressure, partial oxygen tension of pulmonary venous effluent blood, and pulmonary wet-dry weight ratio. The sequential changes in the concentration of NO-related substances (NO-RS) in the serum of reperfused blood were also measured by chemiluminescence. RESULTS: During reperfusion, biphasic increases in NO-RS were observed in all groups. In groups 3 and 4, the increases in NO-RS were significantly lower than those of groups 1 and 2, and pulmonary function deteriorated. CONCLUSION: These data suggest that in order to maintain the endogenous NO-producing ability of preserved lung, the flushing pressure must be less than 20 mmHg.     

Chemical modulation of in situ intrinsic cardiac neurones influences myocardial blood flow in the anaesthetised dog

OBJECTIVE: The aim was to determine whether modulation of intrinsic cardiac neurones influences the distribution of myocardial blood flow in canine anaesthetised open chest experimental preparations. METHODS: Intrinsic cardiac neurones were modified by locally applied nicotine (100 micrograms) or bradykinin (50 micrograms) while changes were recorded in cardiac haemodynamics and myocardial blood flow (radiolabelled microspheres). Right and left ventricular intramyocardial tissue pressures were measured with high fidelity microtip transducers. RESULTS: Control injections of saline (vehicle; 0.1 ml) into active loci did not produce cardiovascular responses. Nicotine modulation of intrinsic cardiac neurones did not change coronary artery conductance, but total myocardial blood flow [116(SEM 17) v 532(97) ml.min-1.100 g-1; p = 0.001 v baseline] and oxygen consumption [7.92(1.10) v 20.14(1.86) ml.min-1.100 g-1; p = 0.001] increased in direct relation to heart rate-blood pressure product changes. Locally administered bradykinin increased coronary artery conductance [2.62(0.39) v 4.71(1.07) ml.min-1.100 g-1.mm Hg-1], total myocardial blood flow, to 263(72) ml.min-1.100 g-1, and oxygen consumption, to 14.9(4.4) ml.min-1.100 g-1; however, heart rate-blood pressure product did not change. CONCLUSIONS: These results support earlier findings that intrinsic neurones are involved in cardiac regulation. Furthermore, modification of intrinsic cardiac neurones by nicotine or bradykinin significantly alters the distribution of myocardial blood flow, possibly because of increased myocardial metabolism.     

Kinetics of the reaction between carbon dioxide and hydrogen over a ruthenium catalyst in a continuous stirred tank reactor

The catalytic reaction between carbon dioxide and hydrogen over a surface coated ruthenium catalyst was investigated in a stirred tank reactor, under isothermal conditions from 200 to 300°C. The kinetic data obtained were analysed by the Hougen-Watson method. Non-linear least squares analysis was used to discriminate among 28 rate models. The following Hougen-Watson type rate equation was selected.     

Speciation and Electronic Structure of La<Subscript>1−x</Subscript>Sr<Subscript>x</Subscript>CoO<Subscript>3−δ</Subscript> During Oxygen Electrolysis

Cobalt-containing perovskite oxides are promising electrocatalysts for the oxygen evolution reaction (OER) in alkaline electrolyzers. However, a lack of fundamental understanding of oxide surfaces impedes rational catalyst design for improved activity and stability. We couple electrochemical studies of epitaxial La_1?xSr_xCoO_3?δ films with in situ and operando ambient pressure X-ray photoelectron spectroscopy to investigate the surface stoichiometry, adsorbates, and electronic structure. In situ investigations spanning electrode compositions in a humid environment indicate that hydroxyl and carbonate affinity increase with Sr content, leading to an increase in binding energy of metal core levels and the valence band edge from the formation of a surface dipole. The maximum in hydroxylation at 40% Sr is commensurate with the highest OER activity, where activity scales with greater hole carrier concentration and mobility. Operando measurements of the 20% Sr-doped oxide in alkaline electrolyte indicate that the surface stoichiometry remains constant during OER, supporting the idea that the oxide electrocatalyst is stable and behaves as a metal, with the voltage drop confined to the electrolyte. Furthermore, hydroxyl and carbonate species are present on the electrode surface even under oxidizing conditions, and may impact the availability of active sites or the binding strength of adsorbed intermediates via adsorbate–adsorbate interactions. For covalent oxides with facile charge transfer kinetics, the accumulation of hydroxyl species with oxidative potentials suggests the rate of reaction could be limited by proton transfer kinetics. This operando insight will help guide modeling of self-consistent oxide electrocatalysts, and highlights the potential importance of carbonates in oxygen electrocatalysis.     

A comparative study of Spark Plasma Sintering (SPS), Hot Isostatic Pressing (HIP) and microwaves sintering techniques on p-type Bi2Te3 thermoelectric properties

The sintering of a synthesized p-type Bi₂Te₃ nano-powder has been investigated by three different techniques. Sintering techniques such as Hot Isostatic Pressing (HIP), microwave sintering and Spark Plasma Sintering (SPS) also known as electric field-assisted sintering technique (FAST) have been compared in terms of sintering parameters i.e. temperature, pressure, and power, microstructure and thermoelectric properties of the prepared ceramics. This study demonstrates that the highest figure of merit ZT has been obtained using microwaves or SPS techniques. Ceramics observations reveal differences in microstructure as well as the presence of intra-granular precipitates in the pellets sintered by the three techniques. We finally conclude about the relationship between properties and microstructure to get optimum thermoelectric materials.     

Biocompatible nanofilm coating by magneto-luminous polymerization of methane

Coating is uniquely feasible means to impart biocompatibility to artificial materials to be used in biology and medicine, because a totally different surface-state could be created. However, it is necessary to understand factors that control “biocompatibility” first. The examination of molecular level factors involved in interfacial interaction revealed that the best surface-state for biocompatibility should contain no chemical functional group. Such a surface-state cannot be created by conventional chemical means, and application of conventional coating could be used in rather limited cases only. Magneto-luminous polymerization of methane yields nanofilm of amorphous carbon, which seems to be the closest to the ideal non-interacting surface. Fundamental factors involved in interactions of polymer surface with surrounding medium, and mechanisms how magneto-luminous polymerization coating yields amorphous carbon nanofilm, which has the least interfacial interaction capability, are described in this paper.