全文获取类型
收费全文 | 451篇 |
免费 | 39篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 2篇 |
化学工业 | 178篇 |
金属工艺 | 3篇 |
机械仪表 | 2篇 |
建筑科学 | 8篇 |
矿业工程 | 2篇 |
能源动力 | 7篇 |
轻工业 | 131篇 |
水利工程 | 1篇 |
石油天然气 | 2篇 |
无线电 | 20篇 |
一般工业技术 | 57篇 |
冶金工业 | 18篇 |
自动化技术 | 60篇 |
出版年
2024年 | 2篇 |
2023年 | 6篇 |
2022年 | 41篇 |
2021年 | 45篇 |
2020年 | 22篇 |
2019年 | 30篇 |
2018年 | 31篇 |
2017年 | 23篇 |
2016年 | 22篇 |
2015年 | 10篇 |
2014年 | 22篇 |
2013年 | 33篇 |
2012年 | 32篇 |
2011年 | 34篇 |
2010年 | 23篇 |
2009年 | 10篇 |
2008年 | 18篇 |
2007年 | 20篇 |
2006年 | 10篇 |
2005年 | 8篇 |
2004年 | 12篇 |
2003年 | 8篇 |
2002年 | 4篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 6篇 |
1997年 | 8篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1991年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
排序方式: 共有491条查询结果,搜索用时 15 毫秒
31.
32.
Cristian A. Acevedo María J. Cornejo Yusser A. Olguín Ronny Vallejos Donald I. Brown 《Food and Bioprocess Technology》2013,6(7):1696-1702
After the death of an animal, cell metabolism is controlled locally. The post-mortem oxygen depletion increases the glycolytic activity and lactate production. However, many mechanisms of post-mortem metabolic regulation have not been fully investigated in beef carcasses. In this work, we studied the post-mortem glycolytic behavior (including lactate dehydrogenase) and three dehydrogenase associated to glycolysis (glycerophosphate dehydrogenase, glucose 6-phosphate dehydrogenase, and glycerol dehydrogenase) by using cytochemistry techniques in three fast-twitch muscles (M. longissimus dorsi, M. semimembranosus, and M. cutaneus trunci) of carcasses stored at 0 °C. Our results indicate that glycolysis depends on the type of muscle. The post-mortem glycolytic flux and lactate dehydrogenase activity of M. cutaneus trunci was the lowest of the three muscles studied. Of the other dehydrogenases analyzed, only glycerophosphate and glycerol dehydrogenase showed clear cytochemical reaction. Glucose 6-phosphate dehydrogenase was not used by muscles very much. The glycerophosphate dehydrogenase was the strongest enzymatic activity correlated to the post-mortem glycolytic flux. In addition, a relationship between glycerophosphate dehydrogenase and glycerol dehydrogenase was detected by using a multiple regression model. This phenomenon was studied by using bioinformatics tools, suggesting that glycerophosphate dehydrogenase could oxidize the glycerol in bovine fast-twitch muscles. 相似文献
33.
Xianfeng Chen Holly J. Corbett Sally R. Yukiko Anthony P. Raphael Emily J. Fairmaid Tarl W. Prow Lorena E. Brown Germain J. P. Fernando Mark A. F. Kendall 《Advanced functional materials》2011,21(3):464-473
Densely packed dry‐coated microprojections are shown to deliver vaccines to targeted locations within the skin that are rich in immune cells, thus inducing protective immune responses against a lethal virus challenge. Selectively limiting the antigen coating to the tips of the projections, which penetrate the skin, would significantly reduce the amount of vaccine required in immunization. In this paper a simple technique, dip‐coating the microprojections, is introduced to meet this goal. By increasing the coating solution viscosity, an otherwise strong capillary action is mitigated and the desired controlled coating length on projections is achieved. Following application to the skin, most of the coated vaccine material is rapidly released from the projections (82.6% in mass within 2 min) to the target locations within the skin strata and a potent immune response is induced when a conventional influenza vaccine (Fluvax) is tested in a mouse model. The utility of this coating approach to a variety of molecules representative of vaccines (e.g., chicken egg ovalbumin (OVA) protein, DNA, and fluorescent dyes) is demonstrated. These collective attributes, together with the simplicity of the approach, position the dip‐coating method for practical utility in large vaccination campaigns. 相似文献
34.
Lorena Perrone Dr. Emmanuelle Mothes Maeva Vignes Anaïs Mockel Cindy Figueroa Marie‐Christine Miquel Dr. Marie‐Lise Maddelein Dr. Peter Faller Prof. Dr. 《Chembiochem : a European journal of chemical biology》2010,11(1):110-118
Amyloid‐β peptides (Aβ) and the protein human serum albumin (HSA) interact in vivo. They are both localised in the blood plasma and in the cerebrospinal fluid. Among other functions, HSA is involved in the transport of the essential metal copper. Complexes between Aβ and copper ions have been proposed to be an aberrant interaction implicated in the development of Alzheimer's disease, where Cu is involved in Aβ aggregation and production of reactive oxygen species (ROS). In the present work, we studied copper‐exchange reaction between Aβ and HSA or the tetrapeptide DAHK (N‐terminal Cu‐binding domain of HSA) and the consequence of this exchange on Aβ‐induced ROS production and cell toxicity. The following results were obtained: 1) HSA and DAHK removed CuII from Aβ rapidly and stoichiometrically, 2) HSA and DAHK were able to decrease Cu‐induced aggregation of Aβ, 3) HSA and DAHK suppressed the catalytic HO. production in vitro and ROS production in neuroblastoma cells generated by Cu–Aβ and ascorbate, 4) HSA and DAHK were able to rescue these cells from the toxicity of Cu–Aβ with ascorbate, 5) DAHK was more potent in ROS suppression and restoration of neuroblastoma cell viability than HSA, in correlation with an easier reduction of CuII–HSA than Cu–DAHK by ascorbate, in vitro. Our data suggest that HSA is able to decrease aberrant CuII–Aβ interaction. The repercussion of the competition between HSA and Aβ to bind Cu in the blood and brain and its relation to Alzheimer's disease are discussed. 相似文献
35.
A.M.P. Sakita E.C. Passamani H. Kumar D.R. Cornejo C.S. Fugivara R.D. Noce A.V. Benedetti 《Materials Chemistry and Physics》2013
The influence of current density, at the interval 5–100 mA cm−2, on the structural and magnetic properties of electrodeposited (Co100−xNix)100−yWy alloys (x = 23–33.5 at. % Ni, y = 1.7–7.3 at. % W) was studied from a glycine-containing bath. W-content decreases with the increase of the current density magnitude. X-ray data have shown stabilization of hexagonal close packed, face centered cubic or a mixture of these structures by modulating the applied cathodic current density, for values lower than 50 mA cm−2. Two structural phase transitions were observed: one from hexagonal close packed to face centered cubic structural transition occurring for a current density of 20 mA cm−2, and another one, from cubic crystalline phase to amorphous state, which happens for values higher than 50 mA cm−2. These structural phase transitions seem to be associated with the W-content as well as average crystalline grain sizes that reduce with increasing the current density value. The grain size effect may explain the face centered cubic stabilization in Co-rich CoNiW alloys, which was initially assumed to be basically due to H-adsorption/incorporation. Magnetic properties of Co-rich CoNiW alloys are strongly modified by the current density value; as a result of the changes on the W-content and their structural properties. 相似文献
36.
In this paper a methodological approach is proposed to validate mechanistic modeling for proton binding onto active sites of mineral and soil samples by reducing the uncertainty and arbitrariness of model schematization. This approach is based on the quantitative formulation (X-ray calibration method) of a simulating mineral mixture (SMM) accounting for the main mineral phases in the soil (quartz, goethite, hematite, muscovite, clinochlore). Mineral and organic contributions were separated by comparing titration curves of river sediment and SMM. Specific mineral contributions to the acid properties of SMM were separated by comparing titration models of SMM and single minerals. Different nonelectrostatic models were used for titrations of SMM and single minerals: two-site/three-KH models (one amphoteric plus one monoprotic site) for clay minerals and SMM; one-site/two-KH models (one amphoteric site) for goethite and hematite; and a one-site/one-KH model (one monoprotic site) for quartz. Crossed-comparisons of titration models allow for identifying and quantifying the specific contributions of the distinct edge hydroxyl groups of iron oxides, clay minerals, and quartz in the different pH ranges. In particularthe amphoteric sites of aluminosilicates mainly contribute in the acid-neutral pH range, the amphoteric sites of iron oxides take part in the neutral-basic range, and finally the monoprotic edge hydroxyl groups of quartz react in the upper basic region of pH. The good simulation of the acid-base properties of SMM (according to single mineral titration models and quantitative composition by X-ray) confirms both model schematization and SMM formulation. Speciation diagrams of the active sites of the different mineral components (aluminosilicates, iron oxides, and quartz) were obtained by implementing the database of a dedicated software with the apparent equilibrium constants regressed by titration modeling of single minerals. 相似文献
37.
Electrocatalytic Detection: Magnetic Bead/Gold Nanoparticle Double‐Labeled Primers for Electrochemical Detection of Isothermal Amplified Leishmania DNA (Small 2/2016)
下载免费PDF全文
![点击此处可从《Small (Weinheim an der Bergstrasse, Germany)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
38.
Gerardo Navarro-García Juan C. Ramírez-Suárez Elizabeth Cota-Quiñones Fernando Márquez-Farías Lorena Bringas-Alvarado 《Food chemistry》2010
The effect of storage time on the quality of liver oil from two commercial rajiform species (Rhinoptera bonasus and Aetobatus narinari) captured in the Gulf of Mexico (State of Campeche coast line) was evaluated. Oil characterisation was conducted by physical (specific gravity, saponification index and water content) and chemical analyses (fatty acid content, carotenes and tocopherols) whilst storage stability (peroxide value, free fatty acids, conjugated dienes, anisidine value and changes in docosahexaenoic acid, DHA and content) was studied for 87 days at 25 °C. Increases (p < 0.05) in free fatty acids, conjugated dienes, peroxide value and anisidine value, and a decrease (p < 0.05) in DHA were observed during the storage time for both oil species as oxidation proceeded. It was concluded that R. bonasus and A. narinari oils lasted for 52 and 66 days under these storage conditions, respectively. 相似文献
39.
The synthesis of nickel aluminium hydroxypicrate, [Ni3Al(OH)8] (C6O7N3H2)·nH
2O, and lithium aluminium hydroxypicrate, [Al2Li(OH)6] (C6O7N3H2)·nH
2O by anion exchange is described. Picric acid and the corresponding hydroxycarbonates were used as starting materials. The new compounds were characterized by chemical analyses, electron microscopy, infrared spectroscopy and X-ray diffraction. The results obtained indicate that both are hydrotalcite-like compounds where the picrate anion lies between the basic layers. The thermal decomposition of the compounds was studied by differential thermal and thermogravimetric analysis. 相似文献
40.
The surface hydrophobicity and functional properties of actomyosin from the mantle of frozen squid caught either by jigging machines (AME1) or by trawl (AME2) were investigated. Two components of 155 and 55 kDa were present in the gels at zero time of storage. Degradation of the myosin heavy chain and increase in the 155 kDa component occur earlier in AME2. Irrespective of the catch method used, no significant (P>0.05) changes in protein solubility were observed. The reduced viscosity of both AME1 and AME2 decreased up to months 3 and 5 of frozen storage, respectively. At the beginning of storage, the superficial hydrophobicity of AME2 was 30% higher than that of AME1. So 1-anilino-8-naphthalene sulfonic acid of AME2 significantly increased during 3-5 months of storage period and that of AME1 at the end of storage. The emulsion activity index (EAI) of AME2 significantly (P<0.05) increased during the first month and decreased after 3 months of storage. EAI of AME1 decreased at month 3 and remained unchanged thereafter. Emulsion stability (ES) of AME2 showed a behavior that was similar to its IAE and that of AME1 remained unchanged. 相似文献