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排序方式: 共有167条查询结果,搜索用时 625 毫秒
81.
82.
Daniela Guarnieri Annarita Falanga Ornella Muscetti Rossella Tarallo Sabato Fusco Massimiliano Galdiero Stefania Galdiero Paolo A. Netti 《Small (Weinheim an der Bergstrasse, Germany)》2013,9(6):853-862
Many therapeutic drugs are excluded from entering the brain due to their lack of transport through the blood–brain barrier (BBB). The development of new strategies for enhancing drug delivery to the brain is of great importance in diagnostics and therapeutics of central nervous diseases. To overcome this problem, a viral fusion peptide (gH625) derived from the glycoprotein gH of Herpes simplex virus type 1 is developed, which possesses several advantages including high cell translocation potency, absence of toxicity of the peptide itself, and the feasibility as an efficient carrier for delivering therapeutics. Therefore, it is hypothesized that brain delivery of nanoparticles conjugated with gH625 should be efficiently enhanced. The surface of fluorescent aminated polystyrene nanoparticles (NPs) is functionalized with gH625 via a covalent binding procedure, and the NP uptake mechanism and permeation across in vitro BBB models are studied. At early incubation times, the uptake of NPs with gH625 by brain endothelial cells is greater than that of the NPs without the peptide, and their intracellular motion is mainly characterized by a random walk behavior. Most importantly, gH625 peptide decreases NP intracellular accumulation as large aggregates and enhances the NP BBB crossing. In summary, these results establish that surface functionalization with gH625 may change NP fate by providing a good strategy for the design of promising carriers to deliver drugs across the BBB for the treatment of brain diseases. 相似文献
83.
Manzoni L Belvisi L Arosio D Bartolomeo MP Bianchi A Brioschi C Buonsanti F Cabella C Casagrande C Civera M De Matteo M Fugazza L Lattuada L Maisano F Miragoli L Neira C Pilkington-Miksa M Scolastico C 《ChemMedChem》2012,7(6):1084-1093
We report the synthesis of novel chelates of Gd and (68)Ga with DPTA, DOTA, HP-DOA3, as well as with AAZTA, a novel chelating agent developed by our research group. These chelating agents were appropriately conjugated, prior to metal complexation, with DB58, an RGD peptidomimetic, conformationally constrained on an azabicycloalkane scaffold and endowed with high affinity for integrin α(ν)β(3) . Because α(ν)β(3) is involved in neo-angiogenesis in solid tumors and is also directly expressed in cancer cells (e.g. glioblastomas, melanomas) and ovarian, breast, and prostate cancers, these constructs could prove useful as molecular imaging probes in cancer diagnosis by MRI or PET techniques. Molecular modeling, integrin binding assays, and relaxivity assessments allowed the selection of compounds suitable for multiple expression on dendrimeric or nanoparticulate structures. These results also led us to an exploratory investigation of (68)Ga complexation for the promising (68)Ga-PET technique; the AAZTA complex 15((68)Ga) exhibited uptake in a xenograft model of glioblastoma, suggesting potentially useful developments with new probes with improved affinity. 相似文献
84.
The paper presents a formulation for two-dimensional anisotropic eddy current problems developed both in the time and the frequency domains. It is based on the cell method, that makes use of integral electromagnetic quantities related to each other through algebraic constitutive and structure equations. This formulation directly assembles both the so-called stiffness and mass matrices by means of an inspection approach with no need to use topological incidence matrices, thus providing an agile construction of the fundamental system and requiring to compute only the primal cell complex, not the dual one. The formulation is compared with an equivalent finite element method method based on the Galerkin and Crank-Nicolson schemes, both being used for simulating an experimental induction-heating apparatus. 相似文献
85.
Nicoletta Villa Serena Redaelli Elena Sala Donatella Conconi Lorenza Romitti Emanuela Manfredini Francesca Crosti Gaia Roversi Marialuisa Lavitrano Ornella Rodeschini Maria Paola Recalcati Rocco Piazza Leda Dalpr Paola Riva Angela Bentivegna 《International journal of molecular sciences》2021,22(11)
The presence of thousands of repetitive sequences makes the centromere a fragile region subject to breakage. In this study we collected 31 cases of rearrangements of chromosome 18, of which 16 involved an acrocentric chromosome, during genetic screening done in three centers. We noticed a significant enrichment of reciprocal translocations between the centromere of chromosome 18 and the centromeric or pericentromeric regions of the acrocentrics. We describe five cases with translocation between chromosome 18 and an acrocentric chromosome, and one case involving the common telomere regions of chromosomes 18p and 22p. In addition, we bring evidence to support the hypothesis that chromosome 18 preferentially recombines with acrocentrics: (i) the presence on 18p11.21 of segmental duplications highly homologous to acrocentrics, that can justify a NAHR mechanism; (ii) the observation by 2D-FISH of the behavior of the centromeric regions of 18 respect to the centromeric regions of acrocentrics in the nuclei of normal subjects; (iii) the contact analysis among these regions on published Hi-C data from the human lymphoblastoid cell line (GM12878). 相似文献
86.
Lorenza Petrini Silvia Necchi Silvio Taschieri Francesco Migliavacca 《Journal of Materials Engineering and Performance》2009,18(5-6):631-637
Ni-Ti rotary endodontic instruments (files) are used in dentistry during the endodontic treatment to shape the root canal of the tooth while removing the pulp when infected. Up to now, the studies for evaluating their performances and drawbacks were mainly limited to experimental tests on product flexural and torsional resistance. This work exploits computational analyses for investigating the effects of materials with different mechanical properties on the behavior of rotary endodontic instruments. The aim is to understand the appropriate material choice to reduce the criticality of the treatment in particular clinical conditions. In particular, the interaction between an accurately modeled rotating file and differently shaped root canals during the clinical procedure was studied performing finite element analyses. Strains induced by the treatment on a file made of a “standard” Ni-Ti alloy (characterized by average properties of the pseudoelastic behavior), a “long” Ni-Ti alloy (characterized by wide transformation region), a “super” Ni-Ti alloy (characterized by an extended Hookian behavior without transformation region), and stainless steel were compared. The results accurately show the advantages of the use of Ni-Ti alloy with respect to stainless steel and the better performance of the “long” alloy in all the tested case. 相似文献
87.
A Boundary Integral Formulation on Unstructured Dual Grids for Eddy-Current Analysis in Thin Shields
A 3-D boundary integral method (3-D BIM) capable of analyzing eddy currents in thin shields is presented. This novel approach is formulated in terms of loop currents in order to implicitly fulfill the div-free condition for quasi-magnetostatic problems. Using nonorthogonal dual grids unknowns are defined on nodes, resulting in a very efficient formulation with limited memory requirements. The proposed method is tested on an axisymmetric model showing a good agreement with the analytical solution. 3-D BIM is then applied to analyze a real case of low-frequency magnetic shielding 相似文献
88.
Enxhi Shaba Claudia Landi Alfonso Carleo Lorenza Vantaggiato Eugenio Paccagnini Mariangela Gentile Laura Bianchi Pietro Lupetti Elena Bargagli Antje Prasse Luca Bini 《International journal of molecular sciences》2021,22(11)
In the longtime challenge of identifying specific, easily detectable and reliable biomarkers of IPF, BALF proteomics is providing interesting new insights into its pathogenesis. To the best of our knowledge, the present study is the first shotgun proteomic investigation of EVs isolated from BALF of IPF patients. Our main aim was to characterize the proteome of the vesicular component of BALF and to explore its individual impact on the pathogenesis of IPF. To this purpose, ultracentrifugation was chosen as the EVs isolation technique, and their purification was assessed by TEM, 2DE and LC-MS/MS. Our 2DE data and scatter plots showed considerable differences between the proteome of EVs and that of whole BALF and of its fluid component. Analysis of protein content and protein functions evidenced that EV proteins are predominantly involved in cytoskeleton remodeling, adenosine signaling, adrenergic signaling, C-peptide signaling and lipid metabolism. Our findings may suggest a wider system involvement in the disease pathogenesis and support the importance of pre-fractioning of complex samples, such as BALF, in order to let low-abundant proteins-mediated pathways emerge. 相似文献
89.
Francesca Diomede Luigia Fonticoli Simone Guarnieri Ylenia Della Rocca Thangavelu Soundara Rajan Antonella Fontana Oriana Trubiani Guya Diletta Marconi Jacopo Pizzicannella 《International journal of molecular sciences》2021,22(14)
Curcumin, a yellow polyphenol extracted from the turmeric root is used as a diet supplement. It exhibits anti-inflammatory, antioxidant, and antitumor properties by modulating different intracellular mechanisms. Due to their low solubility in water, the curcumin molecules must be encapsulated into liposomes to improve the bioavailability and biomedical potential. For the periodontal tissue and systemic health, it is essential to regulate the local inflammatory response. In this study, the possible beneficial effect of liposomes loaded with curcumin (CurLIP) in neural crest-derived human periodontal ligament stem cells (hPDLSCs) and in endothelial-differentiated hPDLSCs (e-hPDLSCs) induced with an inflammatory stimulus (lipopolysaccharide obtained from Porphyromonas gingivalis, LPS-G) was evaluated. The CurLIP formulation exhibited a significant anti-inflammatory effect by the downregulation of Toll-like receptor-4 (TLR4)/Myeloid differentiation primary response 88 (MyD88)/nuclear factor kappa light chain enhancer of activated B cells (NFkB)/NLR Family Pyrin Domain Containing 3 (NLRP3)/Caspase-1/Interleukin (IL)-1β inflammation cascade and reactive oxygen species (ROS) formation. Moreover, the exposure to LPS-G caused significant alterations in the expression of epigenetic modifiers, such as DNA Methyltransferase 1 (DNMT1) and P300, while the CurLIP treatment showed physiological expression. Overall, our in vitro study provides novel mechanistic insights into the intracellular pathway exert by CurLIP in the regulation of inflammation and epigenetic modifications. 相似文献
90.