Maria Bellumori  Lorenzo Cecchi  Annalisa Romani  Nadia Mulinacci  Marzia Innocenti 《Journal of the science of food and agriculture》2018,98(7):2761-2769

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Mauro Arcorace;Andrea Libertino;Lorenzo Alfieri;Simone Gabellani;Alessia Matanò;Alessandro Masoero;Valerio Basso;Giorgio Boni; 《Journal of Flood Risk Management》2024,17(4):e13034

Reliable flood hazard mapping is crucial for enhancing flood preparedness, especially in poorly gauged and vulnerable areas. Yet, current flood models are either highly detailed and computationally intensive or oversimplified, failing to capture important flow dynamics. Here, we present the Rapid Estimation of FLood EXtent (REFLEX), a hydro-geomorphological model for flood hazard mapping at multiple scales that improves upon the existing Height Above the Nearest Drainage model by constraining flood extent using physically-coherent flood volumes. Input volume is distributed from channel to hillslope cells by using an iterative optimization based on the Manning's equation, bypassing the need for accurate rating curves. The model improves the accuracy of inundation extent in flat areas by accounting for backwater effects, and in coastal zones by extending the calculation boundaries beyond the direct watershed area. REFLEX was tested over five areas with different climatic conditions, including flash-flood prone catchments in Italy and large floodplains in the Zambezi river basin. Results denote skillful representation of flooded areas in the Italian catchments, with critical success index (CSI) up to 0.62. In the African cases, REFLEX produced the best estimates of the flood extent and CSI comparable with two state of the art global inundation models. REFLEX is a competitive flood modelling tool for large scale and high resolution applications thanks to its limited input requirements and computing times two orders of magnitude shorter than equivalent hydraulic simulations.  相似文献   

    

Gianni Gerlini  Paola Di Gennaro  Nicola Pimpinelli  Serena Sestini  Lorenzo Borgognoni 《International journal of molecular sciences》2022,23(7)

Langerhans cells (LCs) are crucial regulators of anti-cancer immune responses. Cancer, however, can alter DCs functions leading to tolerance. The enzyme indoleamine 2,3-dioxygenase (IDO1) plays a crucial role in this process. In sentinel lymph nodes (SLNs) of patients with melanoma, LCs show phenotypical and functional alterations favoring tolerance. Herein we aimed to investigate IDO1 expression in SLN LCs from patients with melanoma. We showed by immunofluorescence analysis that a portion of Langerin⁺ LCs, located in the SLN T cell-rich area, displayed the typical dendritic morphology and expressed IDO1. There was no significant difference in the expression of IDO between SLN with or without metastases. Double IDO1/CD83 staining identified four LCs subsets: real mature IDO1⁻CD83⁺ LCs; real immature IDO1⁻CD83⁻ LCs; tolerogenic mature IDO1⁺CD83⁺ LCs; tolerogenic immature IDO1⁺CD83⁻ LCs. The latter subset was significantly increased in metastatic SLNs as compared to negative ones (p < 0.05), and in SLN LCs of patients with mitotic rate (MR) > 1 in primary melanoma, as compared to MR ≤ 1 (p < 0.05). Finally, immature SLN LCs, after in vitro stimulation by inflammatory cytokines, acquired a maturation profile by CD83 up-regulation. These results provide new input for immunotherapeutic approaches targeting in vivo LC of patients with melanoma.  相似文献   

    

Marta Lima  Luciana C. Gomes  Rita Teixeira-Santos  Maria J. Romeu  Jesus Valcarcel  Jos Antonio Vzquez  Miguel A. Cerqueira  Lorenzo Pastrana  Ana I. Bourbon  Ed D. de Jong  Jelmer Sjollema  Filipe J. Mergulho 《International journal of molecular sciences》2022,23(23)

Marine biofouling is a natural process often associated with biofilm formation on submerged surfaces, creating a massive economic and ecological burden. Although several antifouling paints have been used to prevent biofouling, growing ecological concerns emphasize the need to develop new and environmentally friendly antifouling approaches such as bio-based coatings. Chitosan (CS) is a natural polymer that has been widely used due to its outstanding biological properties, including non-toxicity and antimicrobial activity. This work aims to produce and characterize poly (lactic acid) (PLA)-CS surfaces with CS of different molecular weight (Mw) at different concentrations for application in marine paints. Loligo opalescens pens, a waste from the fishery industry, were used as a CS source. The antimicrobial activity of the CS and CS-functionalized surfaces was assessed against Cobetia marina, a model proteobacterium for marine biofouling. Results demonstrate that CS targets the bacterial cell membrane, and PLA-CS surfaces were able to reduce the number of culturable cells up to 68% compared to control, with this activity dependent on CS Mw. The antifouling performance was corroborated by Optical Coherence Tomography since PLA-CS surfaces reduced the biofilm thickness by up to 36%, as well as the percentage and size of biofilm empty spaces. Overall, CS coatings showed to be a promising approach to reducing biofouling in marine environments mimicked in this work, contributing to the valorization of fishing waste and encouraging further research on this topic.  相似文献   

    

Jamal Elhasnaoui  Giulio Ferrero  Valentina Miano  Lorenzo Franchitti  Isabella Tarulli  Lucia Coscujuela Tarrero  Santina Cutrupi  Michele De Bortoli 《International journal of molecular sciences》2022,23(14)

Epithelial splicing regulatory proteins 1 and 2 (ESRP1/2) control the splicing pattern during epithelial to mesenchymal transition (EMT) in a physiological context and in cancer, including breast cancer (BC). Here, we report that ESRP1, but not ESRP2, is overexpressed in luminal BCs of patients with poor prognosis and correlates with estrogen receptor α (ERα) levels. Analysis of ERα genome-binding profiles in cell lines and primary breast tumors showed its binding in the proximity of ESRP1 and ESRP2 genes, whose expression is strongly decreased by ERα silencing in hormone-deprived conditions. The combined knock-down of ESRP1/2 in MCF-7 cells followed by RNA-Seq, revealed the dysregulation of 754 genes, with a widespread alteration of alternative splicing events (ASEs) of genes involved in cell signaling, metabolism, cell growth, and EMT. Functional network analysis of ASEs correlated with ESRP1/2 expression in ERα+ BCs showed RAC1 as the hub node in the protein–protein interactions altered by ESRP1/2 silencing. The comparison of ERα- and ESRP-modulated ASEs revealed 63 commonly regulated events, including 27 detected in primary BCs and endocrine-resistant cell lines. Our data support a functional implication of the ERα-ESRP1/2 axis in the onset and progression of BC by controlling the splicing patterns of related genes.  相似文献   

    

Erika Fiorino  Alessandra Merlini  Lorenzo D&#x;Ambrosio  Ilaria Cerviere  Enrico Berrino  Caterina Marchi  Lidia Giraudo  Marco Basiric  Annamaria Massa  Chiara Donini  Valeria Leuci  Ramona Rotolo  Federica Galvagno  Letizia Vitali  Alessia Proment  Soldano Ferrone  Alberto Pisacane  Ymera Pignochino  Massimo Aglietta  Giovanni Grignani  Giulia Mesiano  Dario Sangiolo 《International journal of molecular sciences》2022,23(18)

Gastrointestinal stromal tumors (GISTs) are rare, mesenchymal tumors of the gastrointestinal tract, characterized by either KIT or PDGFRA mutation in about 85% of cases. KIT/PDGFRA wild type gastrointestinal stromal tumors (wtGIST) account for the remaining 15% of GIST and represent an unmet medical need: their prevalence and potential medical vulnerabilities are not completely defined, and effective therapeutic strategies are still lacking. In this study we set a patient-derived preclinical model of wtGIST to investigate their phenotypic features, along with their susceptibility to cellular immunotherapy with cytokine-induced killer lymphocytes (CIK) and interferons (IFN). We generated 11 wtGIST primary cell lines (wtGISTc). The main CIK ligands (MIC A/B; ULBPs), along with PD-L1/2, were expressed by wtGISTc and the expression of HLA-I molecules was preserved. Patient-derived CIK were capable of intense killing in vitro against wtGISTc resistant to both imatinib and sunitinib. We found that CIK produce a high level of granzyme B, IFNα and IFNγ. CIK-conditioned supernatant was responsible for part of the observed tumoricidal effect, along with positive bystander modulatory activities enhancing the expression of PD-L1/2 and HLA-I molecules. IFNα, but not In, had direct antitumor effects on 50% (4/8) of TKI-resistant wtGISTc, positively correlated with the tumor expression of IFN receptors. wtGIST cells that survived IFNα were still sensitive to CIK immunotherapy. Our data support the exploration of CIK immunotherapy in clinical studies for TKI-resistant wtGIST, proposing reevaluation for IFNα within this challenging setting.  相似文献   

    

Ricard Bou  Paola Navarro-Vozmediano  Rubén Domínguez  Miguel López-Gómez  Montserrat Pinent  Albert Ribas-Agustí  José J. Benedito  José M. Lorenzo  Ximena Terra  José V. García-Pérez  Mirian Pateiro  José A. Herrera-Cervera  Rosa Jorba-Martín 《Comprehensive Reviews in Food Science and Food Safety》2022,21(3):2200-2232

Current demand of consumers for healthy and sustainable food products has led the industry to search for different sources of plant protein isolates and concentrates. Legumes represent an excellent nonanimal protein source with high-protein content. Legume species are distributed in a wide range of ecological conditions, including regions with drought conditions, making them a sustainable crop in a context of global warming. However, their use as human food is limited by the presence of antinutritional factors, such as protease inhibitors, lectins, phytates, and alkaloids, which have adverse nutritional effects. Antitechnological factors, such as fiber, tannins, and lipids, can affect the purity and protein extraction yield. Although most are removed or reduced during alkaline solubilization and isoelectric precipitation processes, some remain in the resulting protein isolates. Selection of appropriate legume genotypes and different emerging and sustainable facilitating technologies, such as high-power ultrasound, pulsed electric fields, high hydrostatic pressure, microwave, and supercritical fluids, can be applied to increase the removal of unwanted compounds. Some technologies can be used to increase protein yield. The technologies can also modify protein structure to improve digestibility, reduce allergenicity, and tune technological properties. This review summarizes recent findings regarding the use of emerging technologies to obtain high-purity protein isolates and the effects on techno-functional properties and health.  相似文献   

    

Francesca Nardecchia  Anna Caciotti  Teresa Giovanniello  Sabrina De Leo  Lorenzo Ferri  Serena Galosi  Silvia Santagata  Barbara Torres  Laura Bernardini  Claudia Carducci  Amelia Morrone  Vincenzo Leuzzi 《International journal of molecular sciences》2022,23(8)

3-Methylglutaconic aciduria type I (MGCA1) is an inborn error of the leucine degradation pathway caused by pathogenic variants in the AUH gene, which encodes 3-methylglutaconyl-coenzyme A hydratase (MGH). To date, MGCA1 has been diagnosed in 19 subjects and has been associated with a variable clinical picture, ranging from no symptoms to severe encephalopathy with basal ganglia involvement. We report the case of a 31-month-old female child referred to our center after the detection of increased 3-hydroxyisovalerylcarnitine levels at newborn screening, which were associated with increased urinary excretion of 3-methylglutaconic acid, 3-hydroxyisovaleric acid, and 3-methylglutaric acid. A next-generation sequencing (NGS) panel for 3-methylglutaconic aciduria failed to establish a definitive diagnosis. To further investigate the strong biochemical indication, we measured MGH activity, which was markedly decreased. Finally, single nucleotide polymorphism array analysis disclosed the presence of two microdeletions in compound heterozygosity encompassing the AUH gene, which confirmed the diagnosis. The patient was then supplemented with levocarnitine and protein intake was slowly decreased. At the last examination, the patient showed mild clumsiness and an expressive language disorder. This case exemplifies the importance of the biochemical phenotype in the differential diagnosis of metabolic diseases and the importance of collaboration between clinicians, biochemists, and geneticists for an accurate diagnosis.  相似文献   

    

Jessica Iorio  Lorenzo Antonuzzo  Emanuela Scarpi  Massimo D&#x;Amico  Claudia Duranti  Luca Messerini  Clotilde Sparano  Damiano Caputo  Daniele Lavacchi  Domenico Borzomati  Alice Antonelli  Lorenzo Nibid  Giuseppe Perrone  Alessandro Coppola  Roberto Coppola  Francesco di Costanzo  Elena Lastraioli  Annarosa Arcangeli 《International journal of molecular sciences》2022,23(18)

hERG1 potassium channels are widely expressed in human cancers of different origins, where they affect several key aspects of cellular behaviour. The present study was designed to evaluate the expression and clinical relevance of hERG1 protein in cancer tissues from patients suffering from neuroendocrine tumours (NETs) of ileal (iNETs) and pancreatic (pNETs) origin, with available clinicopathological history and follow-up. The study was carried out by immunohistochemistry with an anti-hERG1 monoclonal antibody. In a subset of samples, a different antibody directed against the hERG1/β1 integrin complex was also used. The analysis showed for the first time that hERG1 is expressed in human NETs originating from either the ileum or the pancreas. hERG1 turned out to have a prognostic value in NETs, showing (i) a statistically significant positive impact on OS of patients affected by ileal NETs, regardless the TNM stage; (ii) a statistically significant positive impact on OS of patients affected by aggressive (TNM stage IV) disease, either ileal or pancreatic; (iii) a trend to a negative impact on OS of patients affected by less aggressive (TNM stage I-III) disease, either ileal or pancreatic. Moreover, in order to evaluate whether ERG1 was functionally expressed in a cellular model of pNET, the INS1E rat insulinoma cell line was used, and it emerged that blocking ERG1 with a specific inhibitor of the channel (E4031) turned out in a significant reduction in cell proliferation.  相似文献   

    

Enrico Celestino Nista  Sara Sofia De Lucia  Vittoria Manilla  Tommaso Schepis  Antonio Pellegrino  Veronica Ojetti  Giulia Pignataro  Lorenzo Zileri dal Verme  Francesco Franceschi  Antonio Gasbarrini  Marcello Candelli 《International journal of molecular sciences》2022,23(20)

Autoimmune pancreatitis (AIP) is a rare disease. The diagnosis of AIP is difficult and should be made by a comprehensive evaluation of clinical, radiological, serological, and pathological findings. Two different types of AIP have been identified: autoimmune pancreatitis type 1 (AIP-1), which is considered a pancreatic manifestation of multiorgan disease related to IgG4, and autoimmune pancreatitis type 2 (AIP-2), which is considered a pancreas-specific disease not related to IgG4. Although the pathophysiological conditions seem to differ between type 1 and type 2 pancreatitis, both respond well to steroid medications. In this review, we focused on the pathogenesis of the disease to develop a tool that could facilitate diagnosis and lead to the discovery of new therapeutic strategies to combat autoimmune pancreatitis and its relapses. The standard therapy for AIP is oral administration of corticosteroids. Rituximab (RTX) has also been proposed for induction of remission and maintenance therapy in relapsing AIP-1. In selected patients, immunomodulators such as azathioprine are used to maintain remission. The strength of this review, compared with previous studies, is that it focuses on the clear difference between the two types of autoimmune pancreatitis with a clearly delineated and separate pathogenesis. In addition, the review also considers various therapeutic options, including biologic drugs, such as anti-tumor necrosis factor (TNF) therapy, a well-tolerated and effective second-line therapy for AIP type 2 relapses or steroid dependence. Other biologic therapies are also being explored that could provide a useful therapeutic alternative to corticosteroids and immunosuppressants, which are poorly tolerated due to significant side effects.  相似文献