The Divergent Immunomodulatory Effects of Short Chain Fatty Acids and Medium Chain Fatty Acids

Fatty acids are derived from diet and fermentative processes by the intestinal flora. Two to five carbon chain fatty acids, termed short chain fatty acids (SCFA) are increasingly recognized to play a role in intestinal homeostasis. However, the characteristics of slightly longer 6 to 10 carbon, medium chain fatty acids (MCFA), derived primarily from diet, are less understood. Here, we demonstrated that SCFA and MCFA have divergent immunomodulatory propensities. SCFA down-attenuated host pro-inflammatory IL-1β, IL-6, and TNFα response predominantly through the TLR4 pathway, whereas MCFA augmented inflammation through TLR2. Butyric (C4) and decanoic (C10) acid displayed most potent modulatory effects within the SCFA and MCFA, respectively. Reduction in TRAF3, IRF3 and TRAF6 expression were observed with butyric acid. Decanoic acid induced up-regulation of GPR84 and PPARγ and altered HIF-1α/HIF-2α ratio. These variant immune characteristics of the fatty acids which differ by just several carbon atoms may be attributable to their origins, with SCFA being primarily endogenous and playing a physiological role, and MCFA exogenously from the diet.     

Association of IgG1 Antibody Clearance with FcγRIIA Polymorphism and Platelet Count in Infliximab-Treated Patients

The FcγRIIA/CD32A is mainly expressed on platelets, myeloid and several endothelial cells. Its affinity is considered insufficient for allowing significant binding of monomeric IgG, while its H131R polymorphism (histidine > arginine at position 131) influences affinity for multimeric IgG2. Platelet FcγRIIA has been reported to contribute to IgG-containing immune-complexe clearance. Given our finding that platelet FcγRIIA actually binds monomeric IgG, we investigated the role of platelets and FcγRIIA in IgG antibody elimination. We used pharmacokinetics analysis of infliximab (IgG1) in individuals with controlled Crohn’s disease. The influence of platelet count and FcγRIIA polymorphism was quantified by multivariate linear modelling. The infliximab half-life increased with R allele number (13.2, 14.4 and 15.6 days for HH, HR and RR patients, respectively). It decreased with increasing platelet count in R carriers: from ≈20 days (RR) and ≈17 days (HR) at 150 × 10⁹/L, respectively, to ≈13 days (both HR and RR) at 350 × 10⁹/L. Moreover, a flow cytometry assay showed that infliximab and monomeric IgG1 bound efficiently to platelet FcγRIIA H and R allotypes, whereas panitumumab and IgG2 bound poorly to the latter. We propose that infliximab (and presumably any IgG1 antibody) elimination is partly due to an unappreciated mechanism dependent on binding to platelet FcγRIIA, which is probably tuned by its affinity for IgG2.     

Comparison of three techniques used to measure diffusive gas exchange from sheltered aquatic surfaces

Three approaches commonly used to quantify diffusive gas exchange across aquatic surfaces were compared in a densely treed, low-wind environment Diffusive surface fluxes of carbon dioxide (CO2) and methane (CH4) from a small boreal reservoir were estimated using (i) surface water concentrations, the thin boundary layer (TBL) equation, and gas transfer velocities (k) calculated using sulfur hexafluoride (SF6); (ii) surface water concentrations, the TBL equation, and k estimated from wind speed; and (iii) static floating chambers (FCs). Comparisons were made during three different approximately 10-day intervals (August 2000, June and September 2001). CO2 and CH4 fluxes estimated from SF6-derived k were on average 1-3 times greater than those determined from wind-estimated k Overall agreement between FC CO2 and CH4 flux estimates and those based on SF6 and wind speed derived kvalues was much weaker, with FC CO2 and CH4 flux estimates ranging from -9 to 23 times those based on SF6 and wind-estimated k values. Chamber deployment likely enhanced gas transfer through disturbance of the surface boundary layer, and results of this study suggest that caution must be exercised concerning the use of FCs on very still water surfaces. Furthermore, findings of this study contradict the common belief that use of wind speed to approximate k is inappropriate for small bodies of water characterized by low winds and surface obstructions.     

Power assisted fuel cell

A hybrid fuel cell demonstrated pulse power capability at pulse power load simulations synonymous with electronics and communications equipment. The hybrid consisted of a 25.0 W Proton Exchange Membrane Fuel Cell (PEMFC) stack in parallel with a two-cell lead-acid battery. Performance of the hybrid PEMFC was superior to either the battery or fuel cell stack alone at the 18.0 W load. The hybrid delivered a flat discharge voltage profile of about 4.0 V over a 5 h radio continuous transmit mode of 18.0 W.     

Social media and political partisanship – A subaltern public sphere’s role in democracy

Social media, as a subaltern public sphere (Fraser, 1990), have a democratic function in providing an alternative platform for minorities and marginalized to defy mainstream discourses in the public sphere. However, social media have been found to have an echo chamber effect, which may be detrimental to democracy. They may help to accelerate the ascendancy of a “post-truth” era in which objective facts are less influential in shaping public opinion than appeals to emotion and personal belief. A study on political polarization, however, showed that selective exposure and avoidance in social media are weak indicators of polarization (Johnson et al., 2017). This study examines the role of social media in democracy and partisan politics. The authors considered that despite the echo chamber effect, social media have a limited part to play in the formation of polarized stances compared with other factors, such as demographics, political orientation, and mass media use. The study tested two main hypotheses: H1: Social media use is associated with political stance that is marginalized in the mainstream media; H2: Political orientation has a stronger relationship than social media use with the stance toward political values and social issues.The results supported both hypotheses. Social media are associated with political stance that is marginalized in the mainstream media. However, when compared with other factors, the relationship between social media and stance becomes less obvious. Although the echo chamber effect may reinforce the original stance, social media do not exhibit a strong relationship with the stance toward political values and social issues. Partisan orientation and use of partisan mass media are found to have stronger links with variations in stance. Social media, however, provide a subaltern public sphere for those excluded from the dominant public sphere, thus extending the public sphere to accommodate multiple opinions and perspectives.     

Analysis of reactions during sintering of CuO-doped 3Y-TZP nano-powder composites

3Y-TZP (yttria-doped tetragonal zirconia) and CuO nano powders were prepared by co-precipitation and copper oxalate complexation–precipitation techniques, respectively. During sintering of powder compacts (8 mol% CuO-doped 3Y-TZP) of this two-phase system several solid-state reactions clearly influence densification behaviour. These reactions were analysed by several techniques like XPS, DSC/TGA and high-temperature XRD. A strong dissolution of CuO in the 3Y-TZP matrix occurs below 600 °C, resulting in significant enrichment of CuO in a 3Y-TZP grain-boundary layer with a thickness of several nanometres. This “transient” liquid phase strongly enhances densification. Around 860 °C a solid-state reaction between CuO and yttria as segregated to the 3Y-TZP grain boundaries occurs, forming Y₂Cu₂O₅. This solid-state reaction induces the formation of the thermodynamic stable monoclinic zirconia phase. The formation of this solid phase also retards densification. Using this knowledge of microstructural development during sintering it was possible to obtain a dense nano–nano composite with a grain size of only 120 nm after sintering at 960 °C.     

Disponibilité des liaisons hertziennes numériques utilisant des polarisations croisées a la même fréquence

A study is made of the availability of links operating at the same frequencies with cross polarization. A simple method of computation is presented showing how the availability is reduced due to multipath propagation in the case of frequency reutilization. After having pointed out the effect of the characteristics of the antenna with crosspolarization, the reduction is availability of calculated from results of polarization decoupling measurements carried out on various test links.     

A fast algorithm for the hourly simulations of ground-source heat pumps using arbitrary response factors

Hourly energy simulations are an important part of the design and analysis of ground-source heat pump systems. In order to evaluate the fluid temperature in the borehole of a geothermal heat pump system, most of the current models express the heat transfer rate as a sum of step changes in heat transfer rate. The borehole temperature is then computed as a superposition of the different contributions of each time step. The main difference between the different models lies in the way the step response is computed. Since all these methods are based on a convolution scheme, long time simulations are very time consuming. Many load aggregation algorithms have been proposed in order to reduce this computational time. In a previous paper we proposed a new algorithm to evaluate the overall response which was much faster than the classical aggregation schemes. However this new algorithm was based on the cylindrical source step response for a single borehole. In this paper, we present a generalization of this scheme for any kind of step response making it a very powerful tool for hourly simulations.