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991.
Silvio Tundo Giulia Mandal Luca Sella Francesco Favaron Renesh Bedre Raviraj M. Kalunke 《International journal of molecular sciences》2022,23(23)
Xylanase inhibitors (XIs) are plant cell wall proteins largely distributed in monocots that inhibit the hemicellulose degrading activity of microbial xylanases. XIs have been classified into three classes with different structures and inhibition specificities, namely Triticum aestivum xylanase inhibitors (TAXI), xylanase inhibitor proteins (XIP), and thaumatin-like xylanase inhibitors (TLXI). Their involvement in plant defense has been established by several reports. Additionally, these inhibitors have considerable economic relevance because they interfere with the activity of xylanases applied in several agro-industrial processes. Previous reviews highlighted the structural and biochemical properties of XIs and hypothesized their role in plant defense. Here, we aimed to update the information on the genomic organization of XI encoding genes, the inhibition properties of XIs against microbial xylanases, and the structural properties of xylanase-XI interaction. We also deepened the knowledge of XI regulation mechanisms in planta and their involvement in plant defense. Finally, we reported the recently studied strategies to reduce the negative impact of XIs in agro-industrial processes and mentioned their allergenicity potential. 相似文献
992.
Gianni Paulis Giovanni De Giorgio Luca Paulis 《International journal of molecular sciences》2022,23(24)
Background: Peyronie’s disease (PD) is a chronic inflammatory condition affecting adult males, involving the tunica albuginea of the corpora cavernosa of the penis. PD is frequently associated with penile pain, erectile dysfunction, and a secondary anxious–depressive state. The etiology of PD has not yet been completely elucidated, but local injury is generally recognized to be a triggering factor. It has also been widely proven that oxidative stress is an essential, decisive component in all inflammatory processes, whether acute or chronic. Current conservative medical treatment comprises oral substances, penile injections, and physical therapy. Aim: This article intends to show how antioxidant therapy is able to interfere with the pathogenetic mechanisms of the disease. Method: This article consists of a synthetic narrative review of the current scientific literature on antioxidant therapy for this disease. Results: The good results of the antioxidant treatment described above also prove that the doses used were adequate and the concentrations of the substances employed did not exceed the threshold at which they might have interacted negatively with the mechanisms of the redox regulation of tissue. Conclusions: We believe new, randomized, controlled studies are needed to confirm the efficacy of treatment with antioxidants. However, we consider the experiences of antioxidant treatment which can already be found in the literature useful for the clinical practice of urologists in the treatment of this chronic inflammatory disease. 相似文献
993.
Valeria Consoli Valeria Sorrenti Valeria Pittal Khaled Greish Agata Grazia D&#x;Amico Giuseppe Romeo Sebastiano Intagliata Loredana Salerno Luca Vanella 《International journal of molecular sciences》2022,23(10)
The term ferroptosis refers to a peculiar type of programmed cell death (PCD) mainly characterized by extensive iron-dependent lipid peroxidation. Recently, ferroptosis has been suggested as a potential new strategy for the treatment of several cancers, including breast cancer (BC). In particular, among the BC subtypes, triple negative breast cancer (TNBC) is considered the most aggressive, and conventional drugs fail to provide long-term efficacy. In this context, our study’s purpose was to investigate the mechanism of ferroptosis in breast cancer cell lines and reveal the significance of heme oxygenase (HO) modulation in the process, providing new biochemical approaches. HO’s effect on BC was evaluated by MTT tests, gene silencing, Western blot analysis, and measurement of reactive oxygen species (ROS), glutathione (GSH) and lipid hydroperoxide (LOOH) levels. In order to assess HO’s implication, different approaches were exploited, using two distinct HO-1 inducers (hemin and curcumin), a well-known HO inhibitor (SnMP) and a selective HO-2 inhibitor. The data obtained showed HO’s contribution to the onset of ferroptosis; in particular, HO-1 induction seemed to accelerate the process. Moreover, our results suggest a potential role of HO-2 in erastin-induced ferroptosis. In view of the above, HO modulation in ferroptosis can offer a novel approach for breast cancer treatment. 相似文献
994.
Cristina Catt Laura Corte Luca Roscini Gianluigi Cardinali Federica Villa Francesca Cappitelli 《International journal of molecular sciences》2022,23(22)
Zosteric acid (ZA) is a secondary metabolite of the seagrass Zostera marina, with antibiofilm activity against fungi. Information concerning its mechanisms of action is lacking and this limits the development of more potent derivatives based on the same target and activity structure. The aim of this work was to investigate the ZA mode of action by analyzing the metabolic status of Candida albicans biofilm and its protein expression profile upon ZA treatment. Fourier-Transform Infrared Spectroscopy confirmed that ZA modified the metabolomic response of treated cells, showing changes in the spectral regions, mainly related to the protein compartment. Nano Liquid Chromatography–High-Resolution Mass Spectrometry highlighted that 10 proteins were differentially expressed in the C. albicans proteome upon ZA treatment. Proteins involved in the biogenesis, structure and integrity of cell walls as well as adhesion and stable attachment of hyphae were found downregulated, whereas some proteins involved in the stress response were found overexpressed. Additionally, ZA was involved in the modulation of non-DNA-based epigenetic regulatory mechanisms triggered by reactive oxygen species. These results partially clarified the ZA mechanism of action against fungi and provided insight into the major C. albicans pathways responsible for biofilm formation. 相似文献
995.
Luca Grispoldi Musafiri Karama Saeed El-Ashram Cristina Saraiva Juan García-Díez Athanasios Chalias Matteo De Gennis Andrea Vannuccini Giusi Poerio Paolo Torlai Giuseppina Chianese Anna G. Fermani Salvatore Barbera Beniamino T. Cenci-Goga 《Journal of Food Processing and Preservation》2022,46(3):e16351
996.
Luca Magistrelli Elena Contaldi Francesca Vignaroli Silvia Gallo Federico Colombatto Roberto Cantello Cristoforo Comi 《International journal of molecular sciences》2022,23(7)
Parkinson’s disease (PD) is a common neurodegenerative disease characterized by loss of dopaminergic neurons in the pars compacta of the midbrain substantia nigra. PD pathophysiology is complex, multifactorial, and not fully understood yet. Nonetheless, recent data show that immune system hyperactivation with concomitant production of pro-inflammatory cytokines, both in the central nervous system (CNS) and the periphery, is a signature of idiopathic PD. About 5% of PD patients present an early onset with a determined genetic cause, with either autosomal dominant or recessive inheritance. The involvement of immunity in the genetic forms of PD has been a matter of interest in several recent studies. In this review, we will summarize the main findings of this new and promising field of research 相似文献
997.
Luca Hegedüs Elisabeth Livingstone gnes Bnkfalvi Jan Viehof gnes Enyedi gnes Bilecz Balzs Gy&#x;rffy Marcell Baranyi Anna-Mria T&#x;ks Jeovanis Gil Gyrgy Marko-Varga Klaus G. Griewank Lisa Zimmer Renta Vraljai Antje Sucker Anne Zaremba Dirk Schadendorf Clemens Aigner Balzs Hegedüs 《International journal of molecular sciences》2022,23(6)
998.
Pasquale Pisapia Antonino Iaccarino Caterina De Luca Gennaro Acanfora Claudio Bellevicine Roberto Bianco Bruno Daniele Luisa Ciampi Marco De Felice Teresa Fabozzi Luigi Formisano Pasqualina Giordano Cesare Gridelli Giovanni Pietro Ianniello Annamaria Libroia Paolo Maione Mariantonia Nacchio Fabio Pagni Giovanna Palmieri Francesco Pepe Gianluca Russo Maria Salatiello Antonio Santaniello Rachele Scamarcio Davide Seminati Michele Troia Giancarlo Troncone Elena Vigliar Umberto Malapelle 《International journal of molecular sciences》2022,23(15)
Background: Immune-checkpoint inhibitors (ICIs) have increased and improved the treatment options for patients with non-oncogene-addicted advanced stage non-small cell lung cancer (NSCLC). However, the role of ICIs in oncogene-addicted advanced stage NSCLC patients is still debated. In this study, in an attempt to fill in the informational gap on the effect of ICIs on other driver mutations, we set out to provide a molecular landscape of clinically relevant oncogenic drivers in programmed death-ligand 1 (PD-L1) positive NSCLC patients. Methods: We retrospectively reviewed data on 167 advanced stage NSCLC PD-L1 positive patients (≥1%) who were referred to our clinic for molecular evaluation of five driver oncogenes, namely, EGFR, KRAS, BRAF, ALK and ROS1. Results: Interestingly, n = 93 (55.7%) patients showed at least one genomic alteration within the tested genes. Furthermore, analyzing a subset of patients with PD-L1 tumor proportion score (TPS) ≥ 50% and concomitant gene alterations (n = 8), we found that n = 3 (37.5%) of these patients feature clinical benefit with ICIs administration, despite the presence of a concomitant KRAS gene alteration. Conclusions: In this study, we provide a molecular landscape of clinically relevant biomarkers in NSCLC PD-L1 positive patients, along with data evidencing the clinical benefit of ICIs in patient NSCLC PD-L1 positive alterations. 相似文献
999.
Fiorella Calabrese Francesca Lunardi Veronica Tauro Federica Pezzuto Francesco Fortarezza Luca Vedovelli Eleonora Faccioli Elisabetta Balestro Marco Schiavon Giovanni Esposito Stefania Edith Vuljan Chiara Giraudo Dario Gregori Federico Rea Paolo Spagnolo 《International journal of molecular sciences》2022,23(6)
1000.